MD02G1303200.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016408
Physical Location & Seq
Forward (+)
35704126 .. 35704744
619 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1303200.v1.1.491

Sequence Viewer

Length: 537 bp
ATGCAGTACATGATGATGACTACAAACCCATACGAACTCCCTTCTTCATTTGTTCAGCAATGGTATCGTCTCTCAAAAACCTTAGCTGAGCCGGCCGCTTGGAAATTTGCCAAAGGAAATGGGATTGACTTGGTTACAATACATCCAGGGTTTGGGATTGGTCCACTATTACGGCCAACTCTAAATCTCTCCATCAAGTTTTTTCAGAATCTCATCAGTGGTATTGAAACACCGTTTATAAACTACCCATTGGTAGACGTTAGAGATATTGCCTCTGCTCATATTCAAGCATTTGAAGTTCCTTCAGCTAAAGGTAGATATTGTTTAGTTGCCCAAGTCGCTGACGGACCTGATACTCACAAGATTTTGCAACAACTTTACCCCACATTGTGCCTTCGGAGTTCGGCAATCTTTGTGACTCGAAGTTTCAAGTGTCCACAGAGAAAGCAAGAGGTTTGGGAATCACTTTCCTCCCTCTGGAAACGAGTCTCAGACACCGTTGAAAGCCTCAAGGAGAAGGGCTTCCTCAACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.18

Weight (kDa)

9.17

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 239
AccB7I CCANNNNNTGG 1 cut(s) 152
AccI GTMKAC 1 cut(s) 255
AciI CCGC 1 cut(s) 96
AcoI YGGCCR 2 cut(s) 93, 173
AcsI RAATTY 1 cut(s) 104
AcuI CTGAAG 1 cut(s) 288
AdeI CACNNNGTG 1 cut(s) 390
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 152, 477
AgsI TTSAA 5 cut(s) 227, 287, 296, 430, 503
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 2 cut(s) 86, 308
AluI AGCT 2 cut(s) 86, 308
Alw26I GTCTC 2 cut(s) 74, 493
AoxI GGCC 2 cut(s) 93, 173
ApoI RAATTY 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 521
AspS9I GGNCC 2 cut(s) 161, 347
AvaII GGWCC 2 cut(s) 161, 347
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 47, 81
BciT130I CCWGG 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 74, 493
BisI GCNGC 1 cut(s) 96
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 2 cut(s) 161, 347
BmgT120I GGNCC 2 cut(s) 161, 347
BmrFI CCNGG 1 cut(s) 147
Bpu10I CCTNAGC 1 cut(s) 82
Bpu1102I GCTNAGC 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 494
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 477
Bse118I RCCGGY 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 65
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 1 cut(s) 142
BseLI CCNNNNNNNGG 2 cut(s) 152, 477
BseMI GCAATG 1 cut(s) 65
BseMII CTCAG 2 cut(s) 78, 504
BseX3I CGGCCG 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 95, 175
BsiEI CGRYCG 1 cut(s) 96
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 152, 477
BsmAI GTCTC 2 cut(s) 74, 493
BsmBI CGTCTC 1 cut(s) 74
BsnI GGCC 2 cut(s) 95, 175
Bsp1720I GCTNAGC 1 cut(s) 87
BspACI CCGC 1 cut(s) 96
BspANI GGCC 2 cut(s) 95, 175
BspCNI CTCAG 2 cut(s) 79, 503
BsrDI GCAATG 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 234, 499
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 82, 87, 490
BstF5I GGATG 1 cut(s) 142
BstMAI GTCTC 2 cut(s) 74, 493
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 2 cut(s) 92, 338
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BstZI CGGCCG 1 cut(s) 93
BsuRI GGCC 2 cut(s) 95, 175
BtsCI GGATG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 161, 347
Csp6I GTAC 1 cut(s) 7
CviAII CATG 1 cut(s) 10
CviJI RGCY 7 cut(s) 86, 91, 95, 175, 308, 507, 522
CviKI_1 RGCY 7 cut(s) 86, 91, 95, 175, 308, 507, 522
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 3 cut(s) 82, 87, 490
DraIII CACNNNGTG 1 cut(s) 390
EaeI YGGCCR 2 cut(s) 93, 173
EagI CGGCCG 1 cut(s) 93
EclXI CGGCCG 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 161, 347
Eco52I CGGCCG 1 cut(s) 93
Eco57I CTGAAG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 145
Esp3I CGTCTC 1 cut(s) 74
FaeI CATG 1 cut(s) 13
FaiI YATR 4 cut(s) 11, 31, 239, 282
FatI CATG 1 cut(s) 9
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 1 cut(s) 96
FokI GGATG 1 cut(s) 129
Fsp4HI GCNGC 1 cut(s) 96
GluI GCNGC 1 cut(s) 96
HaeIII GGCC 2 cut(s) 95, 175
HapII CCGG 1 cut(s) 92
Hin1II CATG 1 cut(s) 13
HinfI GANTC 4 cut(s) 208, 418, 461, 486
HpaII CCGG 1 cut(s) 92
Hpy166II GTNNAC 3 cut(s) 164, 256, 437
Hpy188I TCNGA 3 cut(s) 207, 399, 493
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 3 cut(s) 164, 256, 437
HpyAV CCTTC 4 cut(s) 51, 312, 404, 511
HpyCH4III ACNGT 2 cut(s) 234, 499
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 4, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 338
HpyF3I CTNAG 3 cut(s) 82, 87, 490
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 1 cut(s) 13
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
LpnPI CCDG 5 cut(s) 105, 132, 159, 363, 463
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 133, 415
MboII GAAGA 1 cut(s) 36
MluCI AATT 1 cut(s) 104
MlyI GAGTC 2 cut(s) 412, 495
MnlI CCTC 6 cut(s) 283, 445, 481, 485, 518, 536
MroNI GCCGGC 1 cut(s) 91
MroXI GAANNNNTTC 1 cut(s) 521
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 92, 338
NaeI GCCGGC 1 cut(s) 93
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 415
PdiI GCCGGC 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 2 cut(s) 208, 461
PflMI CCANNNNNTGG 1 cut(s) 152
PkrI GCNGC 1 cut(s) 97
PleI GAGTC 2 cut(s) 412, 494
PpsI GAGTC 2 cut(s) 412, 494
PsiI TTATAA 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspPI GGNCC 2 cut(s) 161, 347
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 1 cut(s) 14
SatI GCNGC 1 cut(s) 96
Sau96I GGNCC 2 cut(s) 161, 347
SchI GAGTC 2 cut(s) 412, 495
ScrFI CCNGG 1 cut(s) 147
SetI ASST 7 cut(s) 83, 88, 261, 310, 316, 352, 456
SinI GGWCC 2 cut(s) 161, 347
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 1 cut(s) 104
SsiI CCGC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 2 cut(s) 234, 499
TaiI ACGT 1 cut(s) 261
TaqI TCGA 1 cut(s) 421
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 2 cut(s) 208, 461
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 1 cut(s) 36
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 152
VpaK11BI GGWCC 2 cut(s) 161, 347
XapI RAATTY 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.