pycom07g01580

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0016408
Physical Location & Seq
Reverse (-)
1255633 .. 1256352
720 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01580.2

Sequence Viewer

Length: 522 bp
ATGGTACTCGGATCCACTTTTTTGCAAGGATCGATCAAGCAATGGTATCCTCTCTCAAAAACTTTAGCTGAGGAGGCCGCCTGGAAATTTGCCAAAGGAAATGGGATTGACTTGGTAACAATACATCCAGGGTTTGTGATTGGTCCACTCTTACAGCCAAGTCTTAATCTCTCCGTCGAGTTTCTTCTGAATTTAATGAGTGGTATCGAAACACCTTTTATAAACTATGCATTTGTGGATGTTAGAGATGTTGCCTTTGCTCATATTCAAGCATTTGAAGTTCCTTCAGCTAGTGGCAGATATTGTTTAGTTGCACAAGTTGCTGATGGTCCCGATACTCTAAAGATTTTACGAGAGCTTTACCCCACATTGAGCCTTTGTGAGCCTGGCAATCCTTCTGGCTCCAAGTTTCAAGTGTCCAGGGAGAAAGCAAAATGTTTGGGAATCACTTTCCTTCCTCTGGAAACGAGCCTCAAGGACACTGTTGAAAGCCTCATGGAGAAGGGCTTCCTCAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

18.99

Weight (kDa)

5.69

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 221
AciI CCGC 1 cut(s) 78
AclWI GGATC 3 cut(s) 6, 19, 37
AcsI RAATTY 2 cut(s) 86, 190
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 460
AgsI TTSAA 4 cut(s) 269, 278, 413, 488
AjnI CCWGG 4 cut(s) 80, 127, 385, 419
AluBI AGCT 3 cut(s) 68, 290, 358
AluI AGCT 3 cut(s) 68, 290, 358
AlwI GGATC 3 cut(s) 6, 19, 37
AoxI GGCC 1 cut(s) 75
ApoI RAATTY 2 cut(s) 86, 190
Asp700I GAANNNNTTC 1 cut(s) 506
AspS9I GGNCC 2 cut(s) 143, 329
AvaII GGWCC 2 cut(s) 143, 329
BamHI GGATCC 1 cut(s) 11
BbvCI CCTCAGC 1 cut(s) 69
BccI CCATC 1 cut(s) 320
BciT130I CCWGG 4 cut(s) 82, 129, 387, 421
BciVI GTATCC 1 cut(s) 57
BfaI CTAG 1 cut(s) 291
BfuI GTATCC 1 cut(s) 57
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme1390I CCNGG 4 cut(s) 82, 129, 387, 421
Bme18I GGWCC 2 cut(s) 143, 329
BmgT120I GGNCC 2 cut(s) 143, 329
BmiI GGNNCC 3 cut(s) 13, 331, 403
BmrFI CCNGG 4 cut(s) 82, 129, 387, 421
Bpu10I CCTNAGC 1 cut(s) 69
BpuEI CTTGAG 2 cut(s) 458, 497
Bsa29I ATCGAT 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 128, 420
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse3DI GCAATG 1 cut(s) 47
BseBI CCWGG 4 cut(s) 82, 129, 387, 421
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 128, 420
BseGI GGATG 2 cut(s) 124, 244
BseLI CCNNNNNNNGG 1 cut(s) 460
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 1 cut(s) 60
BseRI GAGGAG 1 cut(s) 86
BshFI GGCC 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 32
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 1 cut(s) 460
BsmFI GGGAC 1 cut(s) 315
BsnI GGCC 1 cut(s) 77
Bsp143I GATC 3 cut(s) 11, 29, 33
BspACI CCGC 1 cut(s) 78
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 1 cut(s) 61
BspDI ATCGAT 1 cut(s) 32
BspLI GGNNCC 3 cut(s) 13, 331, 403
BspPI GGATC 3 cut(s) 6, 19, 37
BsrDI GCAATG 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 128, 420
BssMI GATC 3 cut(s) 11, 29, 33
Bst2UI CCWGG 4 cut(s) 82, 129, 387, 421
Bst4CI ACNGT 1 cut(s) 484
BstAPI GCANNNNNTGC 1 cut(s) 320
BstDEI CTNAG 1 cut(s) 69
BstF5I GGATG 2 cut(s) 124, 244
BstKTI GATC 3 cut(s) 14, 32, 36
BstMBI GATC 3 cut(s) 11, 29, 33
BstMWI GCNNNNNNNGC 2 cut(s) 74, 320
BstNI CCWGG 4 cut(s) 82, 129, 387, 421
BstSCI CCNGG 4 cut(s) 80, 127, 385, 419
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 57
BsuRI GGCC 1 cut(s) 77
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 2 cut(s) 124, 244
BtsIMutI CAGTG 1 cut(s) 480
Cfr13I GGNCC 2 cut(s) 143, 329
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 1 cut(s) 5
CviAII CATG 1 cut(s) 496
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 69
DpnI GATC 3 cut(s) 13, 31, 35
DpnII GATC 3 cut(s) 11, 29, 33
Eco47I GGWCC 2 cut(s) 143, 329
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 4 cut(s) 80, 127, 385, 419
EcoT22I ATGCAT 1 cut(s) 232
FaeI CATG 1 cut(s) 499
FaiI YATR 4 cut(s) 221, 228, 264, 497
FaqI GGGAC 1 cut(s) 315
FatI CATG 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 2 cut(s) 111, 251
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 1 cut(s) 291
GluI GCNGC 1 cut(s) 78
HaeIII GGCC 1 cut(s) 77
Hin1II CATG 1 cut(s) 499
HinfI GANTC 1 cut(s) 444
Hpy166II GTNNAC 1 cut(s) 146
Hpy188I TCNGA 2 cut(s) 11, 189
Hpy188III TCNNGA 2 cut(s) 332, 461
Hpy8I GTNNAC 1 cut(s) 146
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 4 cut(s) 294, 405, 464, 496
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4V TGCA 3 cut(s) 25, 230, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 320
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 1 cut(s) 499
Kzo9I GATC 3 cut(s) 11, 29, 33
LmnI GCTCC 1 cut(s) 407
MaeI CTAG 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 115
MalI GATC 3 cut(s) 13, 31, 35
MboI GATC 3 cut(s) 11, 29, 33
MboII GAAGA 1 cut(s) 176
MflI RGATCY 1 cut(s) 11
MluCI AATT 2 cut(s) 86, 190
MnlI CCTC 7 cut(s) 60, 64, 67, 468, 482, 503, 521
Mph1103I ATGCAT 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 2 cut(s) 165, 194
MspR9I CCNGG 4 cut(s) 82, 129, 387, 421
MvaI CCWGG 4 cut(s) 82, 129, 387, 421
MwoI GCNNNNNNNGC 2 cut(s) 74, 320
NdeII GATC 3 cut(s) 11, 29, 33
NlaIII CATG 1 cut(s) 499
NlaIV GGNNCC 3 cut(s) 13, 331, 403
NsiI ATGCAT 1 cut(s) 232
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 444
PkrI GCNGC 1 cut(s) 79
PsiI TTATAA 1 cut(s) 221
Psp6I CCWGG 4 cut(s) 80, 127, 385, 419
PspGI CCWGG 4 cut(s) 80, 127, 385, 419
PspN4I GGNNCC 3 cut(s) 13, 331, 403
PspPI GGNCC 2 cut(s) 143, 329
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SaqAI TTAA 2 cut(s) 165, 194
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 3 cut(s) 11, 29, 33
Sau96I GGNCC 2 cut(s) 143, 329
ScrFI CCNGG 4 cut(s) 82, 129, 387, 421
SetI ASST 4 cut(s) 70, 217, 292, 360
SinI GGWCC 2 cut(s) 143, 329
SmlI CTYRAG 2 cut(s) 473, 512
SmoI CTYRAG 2 cut(s) 473, 512
Sse9I AATT 2 cut(s) 86, 190
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 4 cut(s) 80, 127, 385, 419
TaaI ACNGT 1 cut(s) 484
TaqI TCGA 3 cut(s) 32, 177, 207
TasI AATT 2 cut(s) 86, 190
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 444
Tru1I TTAA 2 cut(s) 165, 194
Tru9I TTAA 2 cut(s) 165, 194
TscAI CASTG 1 cut(s) 487
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 1 cut(s) 487
VpaK11BI GGWCC 2 cut(s) 143, 329
XapI RAATTY 2 cut(s) 86, 190
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 1 cut(s) 291
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.