MD02G1305100.v1.1

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016408
Physical Location & Seq
Forward (+)
35826294 .. 35827455
1162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1305100.v1.1.491

Sequence Viewer

Length: 420 bp
ATGCATCCAGCATATGTGATAGGTCCACTCTTGCAGCCAACACTGAATCTCACCGTGGAGATGATTTGGAATTTCATAACCGGTACAGGTACCGAAACACTACCCCTTTCAAATTACTGGTCCGTTGATGTTAGGGATGTTGCCACTGCTCATATTCTGGCATTTGAAGTTCCTTCAGCAAGTGGAAGATATTGTTTAGTTGCAAATGTTACACATGCTTATGAGGTTCTGAAGATTATACAAGAACCTTATCCCACTTTGCACCTTCCTGAAAAATGTGAGGTTGGCAGTACTTTAGAACCGAAATATCACGTATCCACAGAGAAAGCAAAAAGTTTGGCAAGTAATCTCCTTCCTCTGGAAGTAACTCTCAGGGACACAATTGAAAGCCTGAAGGAGAAGGGCTTCCTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

140

Amino Acids

15.48

Weight (kDa)

5.69

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 89
AcsI RAATTY 1 cut(s) 70
AcuI CTGAAG 3 cut(s) 159, 251, 413
AfaI GTAC 3 cut(s) 85, 91, 292
AfiI CCNNNNNNNGG 1 cut(s) 358
AgeI ACCGGT 1 cut(s) 80
AgsI TTSAA 3 cut(s) 111, 167, 386
ApeKI GCWGC 1 cut(s) 34
ApoI RAATTY 1 cut(s) 70
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AsiGI ACCGGT 1 cut(s) 80
Asp700I GAANNNNTTC 1 cut(s) 404
Asp718I GGTACC 1 cut(s) 89
AspS9I GGNCC 2 cut(s) 23, 120
AsuHPI GGTGA 1 cut(s) 43
AvaII GGWCC 2 cut(s) 23, 120
BanI GGYRCC 1 cut(s) 89
BbvI GCAGC 1 cut(s) 46
BciVI GTATCC 1 cut(s) 325
BfuI GTATCC 1 cut(s) 325
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
BmcAI AGTACT 1 cut(s) 292
Bme18I GGWCC 2 cut(s) 23, 120
BmgT120I GGNCC 2 cut(s) 23, 120
BmiI GGNNCC 1 cut(s) 91
BmsI GCATC 1 cut(s) 13
BpuEI CTTGAG 1 cut(s) 395
BsaAI YACGTR 1 cut(s) 313
BsaJI CCNNGG 1 cut(s) 54
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 1 cut(s) 358
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 2 cut(s) 4, 142
BseLI CCNNNNNNNGG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 385
BseNI ACTGG 1 cut(s) 122
BseXI GCAGC 1 cut(s) 46
BshNI GGYRCC 1 cut(s) 89
BshTI ACCGGT 1 cut(s) 80
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 1 cut(s) 358
BsmFI GGGAC 1 cut(s) 389
BspCNI CTCAG 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 91
BspT107I GGYRCC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 55
BstBAI YACGTR 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 371
BstDSI CCRYGG 1 cut(s) 54
BstF5I GGATG 2 cut(s) 4, 142
BstNSI RCATGY 1 cut(s) 218
BstV1I GCAGC 1 cut(s) 46
BsuI GTATCC 1 cut(s) 325
BtgI CCRYGG 1 cut(s) 54
BtsCI GGATG 2 cut(s) 4, 142
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 2 cut(s) 41, 144
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 23, 120
Csp6I GTAC 3 cut(s) 84, 90, 291
CspAI ACCGGT 1 cut(s) 80
CviAII CATG 1 cut(s) 215
CviJI RGCY 3 cut(s) 37, 390, 405
CviKI_1 RGCY 3 cut(s) 37, 390, 405
CviQI GTAC 3 cut(s) 84, 90, 291
DdeI CTNAG 1 cut(s) 371
Eco47I GGWCC 2 cut(s) 23, 120
Eco57I CTGAAG 3 cut(s) 159, 251, 413
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 1 cut(s) 218
FaiI YATR 7 cut(s) 13, 15, 77, 153, 216, 222, 239
FaqI GGGAC 1 cut(s) 389
FatI CATG 1 cut(s) 214
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 35
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 1 cut(s) 35
GluI GCNGC 1 cut(s) 35
HapII CCGG 1 cut(s) 81
Hin1II CATG 1 cut(s) 218
HinfI GANTC 1 cut(s) 46
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 1 cut(s) 26
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 3 cut(s) 269, 359, 412
Hpy8I GTNNAC 1 cut(s) 26
HpyAV CCTTC 5 cut(s) 183, 275, 362, 388, 394
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 4 cut(s) 4, 34, 203, 262
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 1 cut(s) 218
KpnI GGTACC 1 cut(s) 93
LpnPI CCDG 9 cut(s) 21, 72, 94, 103, 143, 282, 344, 358, 404
Lsp1109I GCAGC 1 cut(s) 46
LweI GCATC 1 cut(s) 13
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 208, 364
MboII GAAGA 2 cut(s) 198, 244
MfeI CAATTG 1 cut(s) 381
MluCI AATT 3 cut(s) 70, 112, 381
MnlI CCTC 4 cut(s) 217, 274, 366, 419
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 404
MslI CAYNNNNRTG 1 cut(s) 219
MspI CCGG 1 cut(s) 81
MunI CAATTG 1 cut(s) 381
NdeI CATATG 1 cut(s) 13
NlaIII CATG 1 cut(s) 218
NlaIV GGNNCC 1 cut(s) 91
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 218
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 1 cut(s) 46
PinAI ACCGGT 1 cut(s) 80
PkrI GCNGC 1 cut(s) 36
Ppu21I YACGTR 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 2 cut(s) 23, 120
RsaI GTAC 3 cut(s) 85, 91, 292
RsaNI GTAC 3 cut(s) 84, 90, 291
RseI CAYNNNNRTG 1 cut(s) 219
SatI GCNGC 1 cut(s) 35
Sau96I GGNCC 2 cut(s) 23, 120
ScaI AGTACT 1 cut(s) 292
SetI ASST 7 cut(s) 25, 91, 228, 250, 267, 285, 315
SfaNI GCATC 1 cut(s) 13
SinI GGWCC 2 cut(s) 23, 120
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 3 cut(s) 70, 112, 381
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 1 cut(s) 315
TasI AATT 3 cut(s) 70, 112, 381
TatI WGTACW 1 cut(s) 290
TfiI GAWTC 1 cut(s) 46
TscAI CASTG 2 cut(s) 48, 151
TseI GCWGC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 64
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 2 cut(s) 48, 151
VpaK11BI GGWCC 2 cut(s) 23, 120
XapI RAATTY 1 cut(s) 70
XceI RCATGY 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 404
ZrmI AGTACT 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.