Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003004

Physical Location & Seq

Genomic Coordinates Reverse (-)

36265111 .. 36279093

Gene Structure 13983 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1307400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1044 bp

ATGTCGAAAGCTGGAGCATTGGATCTCGCATCGGGTCTCGGTGGAAAAATCGACAAAACCGACGTTCTCTCTGCTGTTGAAAAGTATGAGAAGTATCATGTTTGTTATGGAGGGGAAGAGGAAGCAAGGAAGTCCAACTACACTGACATGGTTAATAAATACTATGATCTTGTTACAAGCTTTTATGAGTATGGTTGGGGAGAGTCTTTCCACTTTGCACCCAGGTGGAATGGGGAGTCTCTTCGAGAGAGTATCAAGCGACATGAACACTTCCTTGCTTTGCAACTAGGACTCAAACCTGGGCAGAAAGTTTTGGATGTAGGTTGTGGAATTGGTGGACCACTGAGAGAAATATCTCGCTTCAGCTCAACATCAATTACTGGGTTGAACAACAACGAGTATCAGATCACAAGAGGAAAGGAACTAAACCGTATTGTAGGAGTGGACAAGACCTGCAACTTTGTTAAGGCTGACTTCATGAAACTACCTTTCCCTGAAAATTCATTTGATGCGGTATATGCAATTGAAGCGACCTGCCATGCACCAGATGCATATGGGTGCTACAAGGAGATTTACAGAGTATTAAAACCCGGTCACAGTTTTGCTGCATATGAGTGGTGCATGACTGATGCATTTGATCCCAATAACCAAGAACATCAAAAAATCAAGGCAGAAATTGAGATTGGTGATGGCCTTCCGGACATCAGATTGACAGGAAAGTGTCTTGAAGCTTTGAAACAAGCAGGTTTTGAGGTCATATGGGAGAAAGATCTTGCGGTGGACTCACCTCTTCCATGGTACCTGCCTTTGGACAAAAGTCGGATTTCACTTAGTAGCTTTCGTCTAACTGCTGCTGGGCGATTTGTTACCAGAAACTTGGTCAAGGTTTTAGAATATATTGGACTTGCACCAGCAGGAAGCCAAAGAGTTCAAGATTTTCTGGAGAAAGCAGCTGTAGGGCTAGTCGAAGGCGGAAAGAAAGAGATTTTCACACCGATGTATTTCTTCCTGGCCCGAAAGCCGCTTACGGAGGAGAGTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003838 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005911 GO:0006629 GO:0006694 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008169 GO:0008202 GO:0008610 GO:0008757 GO:0009058 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0012505 GO:0016125 GO:0016126 GO:0016740 GO:0016741 GO:0022414 GO:0030054 GO:0032259 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0061458 GO:0071704 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

CMAS Methyltransf_11 Sterol_MT_C

Protein Analysis

348

Amino Acids

38.98

Weight (kDa)

6.05

Isoelectric Point (pI)

34.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CMAS	PF02353	45 - 255	2.4e-09	Mycolic acid cyclopropane synthetase
Ubie_methyltran	PF01209	91 - 206	2.5e-10	ubiE/COQ5 methyltransferase family
Methyltransf_23	PF13489	92 - 205	2e-08	Methyltransferase domain
Methyltransf_31	PF13847	99 - 223	5e-15	Methyltransferase domain
Methyltransf_25	PF13649	104 - 198	1.5e-17	Methyltransferase domain
Methyltransf_11	PF08241	105 - 200	5e-21	Methyltransferase domain
Methyltransf_12	PF08242	105 - 198	2.4e-08	Methyltransferase domain
Sterol_MT_C	PF08498	278 - 341	2.8e-29	Sterol methyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003004)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13710 AT5G13710
fragaria_vesca	FvH4_7g01390 FvH4_7g01390 FvH4_7g01390
malus_domestica	MD02G1307400.v1.1 MD07G1018200.v1.1
prunus_persica	Prupe.2G019200_v2.0.a1 Prupe.2G019200_v2.0.a1 Prupe.2G019200_v2.0.a1 Prupe.2G019200_v2.0.a1
pyrus_communis	pycom02g25870 pycom07g01400
rosa_chinensis	RchiOBHm_Chr1g0317111 RchiOBHm_Chr3g0482141
rosa_laevigata	RLG00000023373 RLG00000030626
rosa_multiflora	Rmu_sc0000192.1_g000025 Rmu_sc0003689.1_g000012
rosa_roxburghii	Rroxscaffold_4G00330650 Rroxscaffold_6G00399690 Rroxscaffold_6G00400060
rosa_rugosa	Rorug01G0014800 Rorug01G0014900 Rorug01G0014900 Rorug01G0014900 Rorug01G0014900 Rorug03G0194900 Rorug03G0195000
rosa_samantha	Rh1BG022300 Rh1CG024000 Rh1DG047600 Rh3AG246200 Rh3BG281200 Rh3CG277300 Rh3DG273600
rosa_wichuraiana	Rw1G001790 Rw3G022010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	4 cut(s)	461, 542, 734, 810
Acc65I	GGTACC	1 cut(s)	798
AccB1I	GGYRCC	1 cut(s)	798
AccIII	TCCGGA	1 cut(s)	697
AciI	CCGC	4 cut(s)	512, 776, 972, 1022
AclWI	GGATC	2 cut(s)	30, 632
AcsI	RAATTY	1 cut(s)	499
AcuI	CTGAAG	1 cut(s)	346
AfaI	GTAC	1 cut(s)	800
AfiI	CCNNNNNNNGG	1 cut(s)	808
AgsI	TTSAA	6 cut(s)	80, 388, 527, 728, 736, 932
AjnI	CCWGG	3 cut(s)	221, 298, 1008
AjuI	GAANNNNNNNTTGG	2 cut(s)	666, 698
AleI	CACNNNNGTG	1 cut(s)	223
AluBI	AGCT	6 cut(s)	11, 180, 366, 731, 837, 953
AluI	AGCT	6 cut(s)	11, 180, 366, 731, 837, 953
Alw26I	GTCTC	2 cut(s)	41, 243
AlwI	GGATC	2 cut(s)	30, 632
Aor13HI	TCCGGA	1 cut(s)	697
AoxI	GGCC	2 cut(s)	691, 1011
ApeKI	GCWGC	3 cut(s)	605, 851, 950
ApoI	RAATTY	1 cut(s)	499
Asp718I	GGTACC	1 cut(s)	798
AspS9I	GGNCC	2 cut(s)	338, 1012
AsuC2I	CCSGG	1 cut(s)	591
AsuHPI	GGTGA	2 cut(s)	698, 777
AvaII	GGWCC	1 cut(s)	338
BanI	GGYRCC	1 cut(s)	798
BarI	GAAGNNNNNNTAC	2 cut(s)	122, 154
BbvI	GCAGC	3 cut(s)	592, 838, 962
BccI	CCATC	1 cut(s)	683
BciT130I	CCWGG	3 cut(s)	223, 300, 1010
BcnI	CCSGG	1 cut(s)	591
BcoDI	GTCTC	2 cut(s)	41, 243
BfaI	CTAG	2 cut(s)	287, 962
BfmI	CTRYAG	1 cut(s)	954
BfuAI	ACCTGC	4 cut(s)	461, 542, 734, 810
BglII	AGATCT	1 cut(s)	769
BisI	GCNGC	4 cut(s)	606, 852, 951, 1022
BlsI	GCNGC	4 cut(s)	607, 853, 952, 1023
Bme1390I	CCNGG	4 cut(s)	223, 300, 591, 1010
Bme18I	GGWCC	1 cut(s)	338
BmgT120I	GGNCC	2 cut(s)	338, 1012
BmiI	GGNNCC	1 cut(s)	800
BmrFI	CCNGG	4 cut(s)	223, 300, 591, 1010
BmrI	ACTGGG	1 cut(s)	390
BmsI	GCATC	4 cut(s)	38, 499, 538, 619
BmuI	ACTGGG	1 cut(s)	390
BoxI	GACNNNNGTC	1 cut(s)	816
BpmI	CTGGAG	2 cut(s)	33, 962
BpuMI	CCSGG	1 cut(s)	591
BsaI	GGTCTC	1 cut(s)	41
BsaJI	CCNNGG	3 cut(s)	221, 299, 794
BsaWI	WCCGGW	1 cut(s)	697
BsaXI	ACNNNNNCTCC	1 cut(s)	1025
Bsc4I	CCNNNNNNNGG	1 cut(s)	808
Bse1I	ACTGG	1 cut(s)	385
BseAI	TCCGGA	1 cut(s)	697
BseBI	CCWGG	3 cut(s)	223, 300, 1010
BseDI	CCNNGG	3 cut(s)	221, 299, 794
BseGI	GGATG	1 cut(s)	322
BseLI	CCNNNNNNNGG	1 cut(s)	808
BseMII	CTCAG	1 cut(s)	335
BseNI	ACTGG	1 cut(s)	385
BseXI	GCAGC	3 cut(s)	592, 838, 962
BseYI	CCCAGC	1 cut(s)	854
BshFI	GGCC	2 cut(s)	693, 1013
BshNI	GGYRCC	1 cut(s)	798
BsiSI	CCGG	2 cut(s)	591, 698
BslI	CCNNNNNNNGG	1 cut(s)	808
BsmAI	GTCTC	2 cut(s)	41, 243
BsnI	GGCC	2 cut(s)	693, 1013
Bso31I	GGTCTC	1 cut(s)	41
Bsp13I	TCCGGA	1 cut(s)	697
Bsp143I	GATC	5 cut(s)	22, 166, 405, 637, 769
Bsp19I	CCATGG	1 cut(s)	794
BspACI	CCGC	4 cut(s)	512, 776, 972, 1022
BspANI	GGCC	2 cut(s)	693, 1013
BspCNI	CTCAG	1 cut(s)	336
BspEI	TCCGGA	1 cut(s)	697
BspHI	TCATGA	1 cut(s)	477
BspLI	GGNNCC	1 cut(s)	800
BspMI	ACCTGC	4 cut(s)	461, 542, 734, 810
BspPI	GGATC	2 cut(s)	30, 632
BspT107I	GGYRCC	1 cut(s)	798
BspTNI	GGTCTC	1 cut(s)	41
BsrI	ACTGG	1 cut(s)	385
BssECI	CCNNGG	3 cut(s)	221, 299, 794
BssMI	GATC	5 cut(s)	22, 166, 405, 637, 769
BssT1I	CCWWGG	1 cut(s)	794
Bst2UI	CCWGG	3 cut(s)	223, 300, 1010
Bst4CI	ACNGT	2 cut(s)	431, 599
Bst6I	CTCTTC	3 cut(s)	111, 246, 795
BstAPI	GCANNNNNTGC	1 cut(s)	548
BstDEI	CTNAG	2 cut(s)	344, 830
BstDSI	CCRYGG	1 cut(s)	794
BstF5I	GGATG	1 cut(s)	322
BstKTI	GATC	5 cut(s)	25, 169, 408, 640, 772
BstMAI	GTCTC	2 cut(s)	41, 243
BstMBI	GATC	5 cut(s)	22, 166, 405, 637, 769
BstMWI	GCNNNNNNNGC	3 cut(s)	518, 527, 548
BstNI	CCWGG	3 cut(s)	223, 300, 1010
BstPAI	GACNNNNGTC	1 cut(s)	816
BstSCI	CCNGG	4 cut(s)	221, 298, 589, 1008
BstSFI	CTRYAG	1 cut(s)	954
BstV1I	GCAGC	3 cut(s)	592, 838, 962
BstX2I	RGATCY	2 cut(s)	22, 769
BstXI	CCANNNNNNTGG	1 cut(s)	877
BstYI	RGATCY	2 cut(s)	22, 769
BsuRI	GGCC	2 cut(s)	693, 1013
BtgI	CCRYGG	1 cut(s)	794
BtsCI	GGATG	1 cut(s)	322
BtsIMutI	CAGTG	2 cut(s)	141, 341
BveI	ACCTGC	4 cut(s)	461, 542, 734, 810
CciI	TCATGA	1 cut(s)	477
Cfr13I	GGNCC	2 cut(s)	338, 1012
Csp6I	GTAC	1 cut(s)	799
CviAII	CATG	7 cut(s)	98, 148, 263, 478, 539, 622, 795
CviQI	GTAC	1 cut(s)	799
DdeI	CTNAG	2 cut(s)	344, 830
DpnI	GATC	5 cut(s)	24, 168, 407, 639, 771
DpnII	GATC	5 cut(s)	22, 166, 405, 637, 769
Eam1104I	CTCTTC	3 cut(s)	111, 246, 795
EarI	CTCTTC	3 cut(s)	111, 246, 795
EciI	GGCGGA	1 cut(s)	987
Eco130I	CCWWGG	1 cut(s)	794
Eco31I	GGTCTC	1 cut(s)	41
Eco47I	GGWCC	1 cut(s)	338
Eco57I	CTGAAG	1 cut(s)	346
EcoRII	CCWGG	3 cut(s)	221, 298, 1008
EcoT14I	CCWWGG	1 cut(s)	794
EcoT22I	ATGCAT	2 cut(s)	553, 634
ErhI	CCWWGG	1 cut(s)	794
FaeI	CATG	7 cut(s)	101, 151, 266, 481, 542, 625, 798
FalI	AAGNNNNNCTT	2 cut(s)	458, 490
FatI	CATG	7 cut(s)	97, 147, 262, 477, 538, 621, 794
FauNDI	CATATG	3 cut(s)	553, 610, 758
Fnu4HI	GCNGC	4 cut(s)	606, 852, 951, 1022
FokI	GGATG	1 cut(s)	329
Fsp4HI	GCNGC	4 cut(s)	606, 852, 951, 1022
FspBI	CTAG	2 cut(s)	287, 962
GluI	GCNGC	4 cut(s)	606, 852, 951, 1022
GsaI	CCCAGC	1 cut(s)	858
GsuI	CTGGAG	2 cut(s)	33, 962
HaeIII	GGCC	2 cut(s)	693, 1013
HapII	CCGG	2 cut(s)	591, 698
Hin1II	CATG	7 cut(s)	101, 151, 266, 481, 542, 625, 798
HindIII	AAGCTT	2 cut(s)	178, 729
HinfI	GANTC	5 cut(s)	203, 236, 291, 782, 1036
HpaII	CCGG	2 cut(s)	591, 698
HphI	GGTGA	2 cut(s)	698, 777
Hpy166II	GTNNAC	3 cut(s)	338, 445, 781
Hpy188I	TCNGA	3 cut(s)	405, 707, 822
Hpy188III	TCNNGA	6 cut(s)	245, 478, 698, 725, 932, 941
Hpy8I	GTNNAC	3 cut(s)	338, 445, 781
Hpy99I	CGWCG	1 cut(s)	65
HpyAV	CCTTC	2 cut(s)	704, 962
HpyCH4III	ACNGT	2 cut(s)	431, 599
HpyCH4IV	ACGT	1 cut(s)	63
HpyF10VI	GCNNNNNNNGC	3 cut(s)	518, 527, 548
HpyF3I	CTNAG	2 cut(s)	344, 830
HpySE526I	ACGT	1 cut(s)	63
Hsp92II	CATG	7 cut(s)	101, 151, 266, 481, 542, 625, 798
Kpn2I	TCCGGA	1 cut(s)	697
KpnI	GGTACC	1 cut(s)	802
Kzo9I	GATC	5 cut(s)	22, 166, 405, 637, 769
LmnI	GCTCC	1 cut(s)	14
Lsp1109I	GCAGC	3 cut(s)	592, 838, 962
LweI	GCATC	4 cut(s)	38, 499, 538, 619
MaeI	CTAG	2 cut(s)	287, 962
MaeII	ACGT	1 cut(s)	63
MaeIII	GTNAC	3 cut(s)	172, 593, 865
MalI	GATC	5 cut(s)	24, 168, 407, 639, 771
MboI	GATC	5 cut(s)	22, 166, 405, 637, 769
MboII	GAAGA	4 cut(s)	128, 233, 782, 997
MfeI	CAATTG	1 cut(s)	522
MflI	RGATCY	2 cut(s)	22, 769
MluCI	AATT	5 cut(s)	330, 375, 499, 522, 675
MlyI	GAGTC	4 cut(s)	212, 245, 285, 776
MmeI	TCCRAC	2 cut(s)	159, 800
MnlI	CCTC	6 cut(s)	104, 112, 407, 745, 798, 1024
Mph1103I	ATGCAT	2 cut(s)	553, 634
MroI	TCCGGA	1 cut(s)	697
MseI	TTAA	3 cut(s)	153, 465, 584
MslI	CAYNNNNRTG	5 cut(s)	146, 223, 556, 613, 995
MspA1I	CMGCKG	1 cut(s)	953
MspI	CCGG	2 cut(s)	591, 698
MspR9I	CCNGG	4 cut(s)	223, 300, 591, 1010
MunI	CAATTG	1 cut(s)	522
MvaI	CCWGG	3 cut(s)	223, 300, 1010
MwoI	GCNNNNNNNGC	3 cut(s)	518, 527, 548
NciI	CCSGG	1 cut(s)	591
NcoI	CCATGG	1 cut(s)	794
NdeI	CATATG	3 cut(s)	553, 610, 758
NdeII	GATC	5 cut(s)	22, 166, 405, 637, 769
NlaIII	CATG	7 cut(s)	101, 151, 266, 481, 542, 625, 798
NlaIV	GGNNCC	1 cut(s)	800
NmuCI	GTSAC	1 cut(s)	593
NsiI	ATGCAT	2 cut(s)	553, 634
OliI	CACNNNNGTG	1 cut(s)	223
PagI	TCATGA	1 cut(s)	477
PcsI	WCGNNNNNNNCGW	1 cut(s)	57
PkrI	GCNGC	4 cut(s)	607, 853, 952, 1023
PleI	GAGTC	4 cut(s)	211, 244, 285, 776
PpsI	GAGTC	4 cut(s)	211, 244, 285, 776
PshAI	GACNNNNGTC	1 cut(s)	816
Psp6I	CCWGG	3 cut(s)	221, 298, 1008
PspFI	CCCAGC	1 cut(s)	854
PspGI	CCWGG	3 cut(s)	221, 298, 1008
PspN4I	GGNNCC	1 cut(s)	800
PspPI	GGNCC	2 cut(s)	338, 1012
PsuI	RGATCY	2 cut(s)	22, 769
PvuII	CAGCTG	1 cut(s)	953
RsaI	GTAC	1 cut(s)	800
RsaNI	GTAC	1 cut(s)	799
RseI	CAYNNNNRTG	5 cut(s)	146, 223, 556, 613, 995
SaqAI	TTAA	3 cut(s)	153, 465, 584
SatI	GCNGC	4 cut(s)	606, 852, 951, 1022
Sau3AI	GATC	5 cut(s)	22, 166, 405, 637, 769
Sau96I	GGNCC	2 cut(s)	338, 1012
SchI	GAGTC	4 cut(s)	212, 245, 285, 776
ScrFI	CCNGG	4 cut(s)	223, 300, 591, 1010
SfaNI	GCATC	4 cut(s)	38, 499, 538, 619
SfcI	CTRYAG	1 cut(s)	954
SinI	GGWCC	1 cut(s)	338
SmiMI	CAYNNNNRTG	5 cut(s)	146, 223, 556, 613, 995
Sse9I	AATT	5 cut(s)	330, 375, 499, 522, 675
SsiI	CCGC	4 cut(s)	512, 776, 972, 1022
SspMI	CTAG	2 cut(s)	287, 962
StyD4I	CCNGG	4 cut(s)	221, 298, 589, 1008
StyI	CCWWGG	1 cut(s)	794
TaaI	ACNGT	2 cut(s)	431, 599
TaiI	ACGT	1 cut(s)	66
TaqI	TCGA	4 cut(s)	5, 51, 244, 966
TasI	AATT	5 cut(s)	330, 375, 499, 522, 675
TauI	GCSGC	1 cut(s)	1024
Tru1I	TTAA	3 cut(s)	153, 465, 584
Tru9I	TTAA	3 cut(s)	153, 465, 584
TscAI	CASTG	2 cut(s)	148, 348
TseFI	GTSAC	1 cut(s)	593
TseI	GCWGC	3 cut(s)	605, 851, 950
Tsp45I	GTSAC	1 cut(s)	593
TspDTI	ATGAA	4 cut(s)	279, 466, 492, 494
TspGWI	ACGGA	1 cut(s)	1043
TspRI	CASTG	2 cut(s)	148, 348
VpaK11BI	GGWCC	1 cut(s)	338
XapI	RAATTY	1 cut(s)	499
XspI	CTAG	2 cut(s)	287, 962
Zsp2I	ATGCAT	2 cut(s)	553, 634

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.