Rorug03G0195000
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6 SMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003004
Physical Location & Seq
Reverse (-)
17167367 .. 17173506
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0195000.1

Sequence Viewer

Length: 1041 bp
ATGGAGGGTCTCAAGGAGATTCAGAAGCTGGAGGGCCACACTGATAGGGTGTGGAGCCTCGCGTGGAACCCACTCTCCGGTCCCGACGGAGCCCCCCTTAAGCTCGCTTCATGCAGCGGCGATAAGACCGTTCGTATTTGGGAACACAAACCCTCCAACTCCTGGGAATGCCAGGCAGTTCTGGATGATCCGCACACCAGGACCGTCCGATCGTGCGCTTGGTCGCCGTCCGGGAAGCTACTGGCCACTGCCAGCTTCGATGGCACCACCGCAATTTTTCAGGACGTTGGGGATGATTATGAGTGCGTTTCGACCCTAGAGGGTCATGAAAATGAAGTGAAGAGTGTGTCTTGGAATGCGTCCGGGTCTTTGCTTGCGACTTGTGGGAGAGATAAGACTGTGTGGATATGGGAGGTGTTACCCGGCAATGAGTTTGAGTGCGTTGCGGTGTTGCAAGGCCATACGCAAGATGTGAAAATGGTTCAGTGGCATCCGACTATGGAGCTTTTGTTTTCGTGTAGCTATGACAACACTGTCAAGATATGGGCAGATGATGGTGAGGATGATAATTGGGTGTGTGTGCAAACGTTAGCTCAATCTAACAGTGGTCACTCTTCTACTGTATGGGCTCTATCGTTTAATGCTACTGGAGACAAAATGGTTACCTGCAGTGATGATCTTACCCTAAAGATATGGGGAACAGACAACGGGAAGATGCTATCTTCTGATGGCTTTGCACCTTGGAGTCATCTTTGCACTGTTGCGGGTTATCATGATAGGACAATATTTTCAGTTCATTGGTCAAGGGACAATATCATTGCCAGTGGAGCTGCTGATGATACCATAAGATTCTTTGTGGAGAATAATGAGTGTGATAGTTTGGTTGATGGACCTTCCTATAATTTGCTTCTGAAGAAGGAGAAGGCCCATGACATGGATATAAATTCGGTGCAGTGGAGCCCTGGGGAGAGTAGGCTTCTTGCTTCTGCAGCTGATGACGGGACGATTAAAATATGGGAGCTGACATCTGTAAGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130
Pfam Domains
Protein Families
Methyltransf_11 Sterol_MT_C

Protein Analysis

346

Amino Acids

38.19

Weight (kDa)

4.66

Isoelectric Point (pI)

28.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 4 - 93 1.9e-12 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 5 - 85 1.9e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 5 - 47 3.3e-07 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 5 - 139 8.5e-13 WDHD1 first WD40 domain
WD40_Prp19 PF24814 6 - 139 1.7e-17 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 6 - 234 1.4e-25 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 6 - 52 5.9e-07 THOC3 beta-propeller domain
WD40_Gbeta PF25391 9 - 233 2.6e-11 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 18 - 183 5.7e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 29 - 114 8.2e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 56 - 92 1.2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 58 - 232 1.1e-27 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 64 - 241 8.3e-36 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 65 - 118 8.6e-09 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 65 - 242 4.4e-19 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 66 - 183 2.7e-06 WDR36/Utp21 first beta-propeller
Beta-prop_EIPR1 PF23609 66 - 182 3.1e-06 EIPR1 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 67 - 239 7.1e-11 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 97 - 234 6e-12 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 100 - 183 1.4e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 101 - 137 1.3e-10 WD domain, G-beta repeat
EIF3I PF24805 105 - 183 2.9e-08 EIF3I
Beta-prop_Aladin PF25460 106 - 213 6.2e-08 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 109 - 183 2.7e-19 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 125 - 240 2e-09 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 144 - 206 7.9e-06 WDHD1 first WD40 domain
WD40 PF00400 146 - 182 1.1e-09 WD domain, G-beta repeat
WD40_Prp19 PF24814 146 - 241 4.8e-15 Prp19 WD40 domain
EIF3I PF24805 150 - 340 8.3e-10 EIF3I
WD40 PF00400 193 - 232 5.9e-07 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 200 - 344 4.8e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 208 - 342 3.3e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 235 - 343 2.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 258 - 345 2.7e-09 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 258 - 344 3.8e-11 THOC3 beta-propeller domain
WD40 PF00400 307 - 339 1.1e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 263
AccB7I CCANNNNNTGG 2 cut(s) 162, 934
AccII CGCG 1 cut(s) 62
AciI CCGC 5 cut(s) 117, 191, 270, 446, 764
AclI AACGTT 1 cut(s) 587
AclWI GGATC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 943
AcuI CTGAAG 1 cut(s) 932
AfiI CCNNNNNNNGG 3 cut(s) 77, 162, 934
AflII CTTAAG 1 cut(s) 98
AjnI CCWGG 4 cut(s) 161, 171, 197, 961
AloI GAACNNNNNNTCC 2 cut(s) 59, 91
Alw26I GTCTC 2 cut(s) 14, 645
AlwI GGATC 1 cut(s) 182
AlwNI CAGNNNCTG 1 cut(s) 28
AoxI GGCC 4 cut(s) 34, 243, 457, 924
ApeKI GCWGC 4 cut(s) 114, 830, 989, 1035
ApoI RAATTY 1 cut(s) 943
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 5 cut(s) 34, 80, 201, 890, 925
AsuC2I CCSGG 3 cut(s) 232, 364, 423
AsuHPI GGTGA 1 cut(s) 569
AvaII GGWCC 3 cut(s) 80, 201, 890
BalI TGGCCA 1 cut(s) 245
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 3 cut(s) 94, 631, 962
BbvI GCAGC 4 cut(s) 126, 817, 1001, 1022
BccI CCATC 4 cut(s) 254, 548, 722, 881
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 4 cut(s) 163, 173, 199, 963
BcnI CCSGG 3 cut(s) 232, 364, 423
BcoDI GTCTC 2 cut(s) 14, 645
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 2 cut(s) 667, 987
BfrI CTTAAG 1 cut(s) 98
BfuAI ACCTGC 1 cut(s) 674
BisI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
BlsI GCNGC 5 cut(s) 116, 119, 832, 991, 1037
Bme1390I CCNGG 7 cut(s) 163, 173, 199, 232, 364, 423, 963
Bme18I GGWCC 3 cut(s) 80, 201, 890
BmgT120I GGNCC 5 cut(s) 34, 80, 201, 890, 925
BmiI GGNNCC 6 cut(s) 56, 68, 82, 91, 265, 959
BmrFI CCNGG 7 cut(s) 163, 173, 199, 232, 364, 423, 963
BmsI GCATC 2 cut(s) 499, 705
BpmI CTGGAG 2 cut(s) 50, 669
BpuMI CCSGG 3 cut(s) 232, 364, 423
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 4 cut(s) 162, 740, 961, 962
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 137, 167, 494, 524
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 162, 934
Bse1I ACTGG 3 cut(s) 246, 652, 822
Bse3DI GCAATG 2 cut(s) 433, 816
BseBI CCWGG 4 cut(s) 163, 173, 199, 963
BseDI CCNNGG 4 cut(s) 162, 740, 961, 962
BseGI GGATG 4 cut(s) 190, 298, 490, 568
BseLI CCNNNNNNNGG 3 cut(s) 77, 162, 934
BseMI GCAATG 2 cut(s) 433, 816
BseNI ACTGG 3 cut(s) 246, 652, 822
BseXI GCAGC 4 cut(s) 126, 817, 1001, 1022
BsgI GTGCAG 1 cut(s) 971
Bsh1236I CGCG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 4 cut(s) 36, 245, 459, 926
BshNI GGYRCC 1 cut(s) 263
BsiEI CGRYCG 1 cut(s) 212
BsiSI CCGG 4 cut(s) 78, 231, 363, 423
BslFI GGGAC 3 cut(s) 66, 821, 1015
BslI CCNNNNNNNGG 3 cut(s) 77, 162, 934
BsmAI GTCTC 2 cut(s) 14, 645
BsmFI GGGAC 3 cut(s) 66, 821, 1015
BsmI GAATGC 2 cut(s) 173, 361
BsnI GGCC 4 cut(s) 36, 245, 459, 926
Bso31I GGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 3 cut(s) 94, 631, 962
Bsp143I GATC 3 cut(s) 187, 209, 676
BspACI CCGC 5 cut(s) 117, 191, 270, 446, 764
BspANI GGCC 4 cut(s) 36, 245, 459, 926
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 2 cut(s) 325, 772
BspLI GGNNCC 6 cut(s) 56, 68, 82, 91, 265, 959
BspMAI CTGCAG 2 cut(s) 671, 991
BspMI ACCTGC 1 cut(s) 674
BspPI GGATC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 263
BspTI CTTAAG 1 cut(s) 98
BspTNI GGTCTC 1 cut(s) 14
BsrDI GCAATG 2 cut(s) 433, 816
BsrI ACTGG 3 cut(s) 246, 652, 822
BssECI CCNNGG 4 cut(s) 162, 740, 961, 962
BssMI GATC 3 cut(s) 187, 209, 676
BssT1I CCWWGG 1 cut(s) 740
Bst2UI CCWGG 4 cut(s) 163, 173, 199, 963
Bst4CI ACNGT 7 cut(s) 130, 205, 400, 535, 605, 622, 760
Bst6I CTCTTC 2 cut(s) 335, 619
BstAFI CTTAAG 1 cut(s) 98
BstC8I GCNNGC 3 cut(s) 105, 253, 375
BstEII GGTNACC 1 cut(s) 661
BstF5I GGATG 4 cut(s) 190, 298, 490, 568
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 3 cut(s) 190, 212, 679
BstMAI GTCTC 2 cut(s) 14, 645
BstMBI GATC 3 cut(s) 187, 209, 676
BstMCI CGRYCG 1 cut(s) 212
BstMWI GCNNNNNNNGC 3 cut(s) 261, 827, 989
BstNI CCWGG 4 cut(s) 163, 173, 199, 963
BstPI GGTNACC 1 cut(s) 661
BstSCI CCNGG 7 cut(s) 161, 171, 197, 230, 362, 421, 961
BstSFI CTRYAG 2 cut(s) 667, 987
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 4 cut(s) 126, 817, 1001, 1022
BsuRI GGCC 4 cut(s) 36, 245, 459, 926
BtsCI GGATG 4 cut(s) 190, 298, 490, 568
BtsI GCAGTG 3 cut(s) 246, 676, 959
BtsIMutI CAGTG 9 cut(s) 39, 246, 491, 531, 610, 676, 756, 829, 959
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 3 cut(s) 105, 253, 375
CaiI CAGNNNCTG 1 cut(s) 28
CciI TCATGA 2 cut(s) 325, 772
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 5 cut(s) 34, 80, 201, 890, 925
CseI GACGC 1 cut(s) 348
CviAII CATG 5 cut(s) 111, 326, 773, 929, 934
DpnI GATC 3 cut(s) 189, 211, 678
DpnII GATC 3 cut(s) 187, 209, 676
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 335, 619
EarI CTCTTC 2 cut(s) 335, 619
Eco130I CCWWGG 1 cut(s) 740
Eco24I GRGCYC 3 cut(s) 94, 631, 962
Eco31I GGTCTC 1 cut(s) 14
Eco47I GGWCC 3 cut(s) 80, 201, 890
Eco57I CTGAAG 1 cut(s) 932
Eco91I GGTNACC 1 cut(s) 661
EcoO65I GGTNACC 1 cut(s) 661
EcoRII CCWGG 4 cut(s) 161, 171, 197, 961
EcoT14I CCWWGG 1 cut(s) 740
EcoT38I GRGCYC 3 cut(s) 94, 631, 962
ErhI CCWWGG 1 cut(s) 740
FaeI CATG 5 cut(s) 114, 329, 776, 932, 937
FaqI GGGAC 3 cut(s) 66, 821, 1015
FatI CATG 5 cut(s) 110, 325, 772, 928, 933
FauI CCCGC 1 cut(s) 757
Fnu4HI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
FokI GGATG 4 cut(s) 197, 305, 477, 575
FriOI GRGCYC 3 cut(s) 94, 631, 962
Fsp4HI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
FspBI CTAG 1 cut(s) 317
GlaI GCGC 1 cut(s) 217
GluI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
GsuI CTGGAG 2 cut(s) 50, 669
HaeIII GGCC 4 cut(s) 36, 245, 459, 926
HapII CCGG 4 cut(s) 78, 231, 363, 423
HgaI GACGC 1 cut(s) 348
HhaI GCGC 1 cut(s) 218
Hin1II CATG 5 cut(s) 114, 329, 776, 932, 937
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HinfI GANTC 3 cut(s) 19, 745, 849
HpaII CCGG 4 cut(s) 78, 231, 363, 423
HphI GGTGA 1 cut(s) 569
Hpy188I TCNGA 5 cut(s) 24, 209, 495, 727, 912
Hpy188III TCNNGA 6 cut(s) 83, 182, 281, 326, 538, 773
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 3 cut(s) 903, 910, 916
HpyCH4III ACNGT 7 cut(s) 130, 205, 400, 535, 605, 622, 760
HpyCH4IV ACGT 2 cut(s) 285, 587
HpyCH4V TGCA 8 cut(s) 114, 454, 583, 669, 737, 756, 952, 989
HpyF10VI GCNNNNNNNGC 3 cut(s) 261, 827, 989
HpySE526I ACGT 2 cut(s) 285, 587
Hsp92II CATG 5 cut(s) 114, 329, 776, 932, 937
HspAI GCGC 1 cut(s) 216
Kzo9I GATC 3 cut(s) 187, 209, 676
LmnI GCTCC 6 cut(s) 54, 89, 502, 827, 957, 1018
Lsp1109I GCAGC 4 cut(s) 126, 817, 1001, 1022
LweI GCATC 2 cut(s) 499, 705
MaeI CTAG 1 cut(s) 317
MaeII ACGT 2 cut(s) 285, 587
MaeIII GTNAC 3 cut(s) 417, 608, 661
MalI GATC 3 cut(s) 189, 211, 678
MboI GATC 3 cut(s) 187, 209, 676
MboII GAAGA 5 cut(s) 352, 606, 714, 724, 925
MhlI GDGCHC 3 cut(s) 94, 631, 962
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 4 cut(s) 273, 568, 901, 943
MluNI TGGCCA 1 cut(s) 245
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 2 cut(s) 180, 518
MnlI CCTC 6 cut(s) 25, 68, 163, 313, 406, 553
Mox20I TGGCCA 1 cut(s) 245
MscI TGGCCA 1 cut(s) 245
MseI TTAA 3 cut(s) 99, 639, 1008
MslI CAYNNNNRTG 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 245
MspA1I CMGCKG 2 cut(s) 117, 992
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 4 cut(s) 78, 231, 363, 423
MspR9I CCNGG 7 cut(s) 163, 173, 199, 232, 364, 423, 963
Mva1269I GAATGC 2 cut(s) 173, 361
MvaI CCWGG 4 cut(s) 163, 173, 199, 963
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 3 cut(s) 261, 827, 989
NciI CCSGG 3 cut(s) 232, 364, 423
NdeII GATC 3 cut(s) 187, 209, 676
NlaIII CATG 5 cut(s) 114, 329, 776, 932, 937
NlaIV GGNNCC 6 cut(s) 56, 68, 82, 91, 265, 959
NmuCI GTSAC 1 cut(s) 608
PagI TCATGA 2 cut(s) 325, 772
PasI CCCWGGG 1 cut(s) 962
PctI GAATGC 2 cut(s) 173, 361
PfeI GAWTC 2 cut(s) 19, 849
PflMI CCANNNNNTGG 2 cut(s) 162, 934
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 5 cut(s) 116, 119, 832, 991, 1037
Ple19I CGATCG 1 cut(s) 212
PleI GAGTC 1 cut(s) 753
PpsI GAGTC 1 cut(s) 753
Psp1406I AACGTT 1 cut(s) 587
Psp6I CCWGG 4 cut(s) 161, 171, 197, 961
PspEI GGTNACC 1 cut(s) 661
PspGI CCWGG 4 cut(s) 161, 171, 197, 961
PspN4I GGNNCC 6 cut(s) 56, 68, 82, 91, 265, 959
PspPI GGNCC 5 cut(s) 34, 80, 201, 890, 925
PstI CTGCAG 2 cut(s) 671, 991
PstNI CAGNNNCTG 1 cut(s) 28
PvuI CGATCG 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 992
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 3 cut(s) 99, 639, 1008
SatI GCNGC 5 cut(s) 115, 118, 831, 990, 1036
Sau3AI GATC 3 cut(s) 187, 209, 676
Sau96I GGNCC 5 cut(s) 34, 80, 201, 890, 925
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 7 cut(s) 163, 173, 199, 232, 364, 423, 963
SduI GDGCHC 3 cut(s) 94, 631, 962
SfaNI GCATC 2 cut(s) 499, 705
SfcI CTRYAG 2 cut(s) 667, 987
SinI GGWCC 3 cut(s) 80, 201, 890
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 2 cut(s) 11, 98
SmoI CTYRAG 2 cut(s) 11, 98
Sse9I AATT 4 cut(s) 273, 568, 901, 943
SsiI CCGC 5 cut(s) 117, 191, 270, 446, 764
SspI AATATT 1 cut(s) 786
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 7 cut(s) 161, 171, 197, 230, 362, 421, 961
StyI CCWWGG 1 cut(s) 740
TaaI ACNGT 7 cut(s) 130, 205, 400, 535, 605, 622, 760
TaiI ACGT 2 cut(s) 288, 590
TaqI TCGA 2 cut(s) 258, 311
TasI AATT 4 cut(s) 273, 568, 901, 943
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 2 cut(s) 19, 849
Tru1I TTAA 3 cut(s) 99, 639, 1008
Tru9I TTAA 3 cut(s) 99, 639, 1008
TscAI CASTG 9 cut(s) 46, 253, 491, 538, 610, 676, 763, 829, 959
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 4 cut(s) 114, 830, 989, 1035
Tsp45I GTSAC 1 cut(s) 608
TspDTI ATGAA 4 cut(s) 99, 342, 348, 785
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 9 cut(s) 46, 253, 491, 538, 610, 676, 763, 829, 959
Van91I CCANNNNNTGG 2 cut(s) 162, 934
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 3 cut(s) 80, 201, 890
XapI RAATTY 1 cut(s) 943
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.