Rroxscaffold_6G00400060
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6 SMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0003004
Physical Location & Seq
Reverse (-)
22151175 .. 22155194
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00400060.1

Sequence Viewer

Length: 1023 bp
ATGTCAAAATCTGGAGCACTGGATCTGGCTTCTGGGGTTGGCGGGAAGCTTGAGAAGCACCAAGTTCTTTCAGCTGTCCACAAATATGAGAAGTATCACTTTGGGGATGAGGAAGACAGAAAAGCCAACTATACTGACATGGTAAACAAATACTATGATCTCGTCACAAGCTTTTATGAATATGGATGGGGTGAGTCCTTCCATTTTGCACCAAGATGGAAGCAAGAGTCCCTAAAAGAGAGCATCAAGCGGCATGAGCATTTTCTCGCTCTGCAACTGGGTCTAAAGCAAGGGCATAAGGTTTTGGATGTAGGATGTGGGATTGGTGGACCTATGAGAGAAATTACTAGATTCAGTGGGGCATCAGTTACAGGGATAAACAACAACGAATATCAAATAACTAGAGCAAAGGTGAGTGACAGTTTCTTTGGAATTTACTTTACGTTAATGATCATGCAGGCAGACTTTATGAACATGCCATTTCCTGATAACACTTTTGATGCAATATTTGCAATAGAAGCCACATGCCATGCACCGAATGTGTTTGATTGCTACAAAGAAATATATCGAGTGTTGAAGCCAGGACAGTGTTTTGCTGCTTATGAGTGGTGCATTACTGACTCCTTTGATCGAACAAACCATGAACACCAGAGAATTAAGGGGGAAGTTGAGCTTGGCAATGGACTTCCTGATATCAGATCAAAAGAACAATGTATTCACGCTCTAAATGTTGCAGGTTTTGAGGTTATATGGGAAAATGATGTTTCTGTGGGTTCACCGCTTCCTTGGTATATGCCACTAGATAAAAGTCACATTTCATTGAGTAGCTTCCGGCTTACAGCTTTGGGGCGTTTCATTACTAGAACAATGGTCAAAGCCTTGGAGTTTTCACGCCTTGCCCCTGAGGGTAGCCAAAGAGTTCAGTCTTTCCTAGAGACAACAGCAGAAGCTCTTGTTGAAGGTGGAAGGAAAGGGATCTTCACACCAATGTACTTCTTCTTGGTTCGAAAGCCTGATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130
Pfam Domains
Protein Families
Methyltransf_11 Sterol_MT_C

Protein Analysis

340

Amino Acids

38.67

Weight (kDa)

6.32

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 42 - 230 1.4e-10 Mycolic acid cyclopropane synthetase
Ubie_methyltran PF01209 88 - 202 2e-08 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 89 - 250 1.2e-08 Methyltransferase domain
Methyltransf_31 PF13847 96 - 217 3.4e-13 Methyltransferase domain
Methyltransf_25 PF13649 101 - 195 2.4e-16 Methyltransferase domain
Methyltransf_11 PF08241 102 - 197 6e-20 Methyltransferase domain
Methyltransf_12 PF08242 102 - 195 1.4e-07 Methyltransferase domain
Sterol_MT_C PF08498 275 - 338 1.9e-27 Sterol methyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 725
AciI CCGC 3 cut(s) 42, 250, 779
AclWI GGATC 2 cut(s) 30, 983
AcsI RAATTY 1 cut(s) 432
AfaI GTAC 1 cut(s) 992
AgsI TTSAA 2 cut(s) 577, 959
AjnI CCWGG 1 cut(s) 580
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AluBI AGCT 7 cut(s) 49, 74, 171, 673, 828, 842, 950
AluI AGCT 7 cut(s) 49, 74, 171, 673, 828, 842, 950
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 1 cut(s) 929
AlwI GGATC 2 cut(s) 30, 983
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 1 cut(s) 432
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 3 cut(s) 203, 424, 768
AsuII TTCGAA 1 cut(s) 1006
AvaII GGWCC 1 cut(s) 329
AxyI CCTNAGG 1 cut(s) 903
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 583
BccI CCATC 2 cut(s) 180, 210
BciT130I CCWGG 1 cut(s) 582
BclI TGATCA 1 cut(s) 450
BcoDI GTCTC 1 cut(s) 929
BfaI CTAG 5 cut(s) 348, 402, 800, 861, 932
BfuAI ACCTGC 1 cut(s) 725
BisI GCNGC 2 cut(s) 251, 597
BlsI GCNGC 2 cut(s) 252, 598
Bme1390I CCNGG 1 cut(s) 582
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmrFI CCNGG 1 cut(s) 582
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 4 cut(s) 252, 371, 490, 1006
BmuI ACTGGG 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 33
Bpu14I TTCGAA 1 cut(s) 1006
BpuEI CTTGAG 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 785, 879
Bse1I ACTGG 2 cut(s) 24, 282
Bse21I CCTNAGG 1 cut(s) 903
Bse3DI GCAATG 1 cut(s) 685
BseBI CCWGG 1 cut(s) 582
BseDI CCNNGG 2 cut(s) 785, 879
BseGI GGATG 4 cut(s) 112, 191, 313, 320
BseMI GCAATG 1 cut(s) 685
BseMII CTCAG 1 cut(s) 894
BseNI ACTGG 2 cut(s) 24, 282
BseXI GCAGC 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 1 cut(s) 832
BslFI GGGAC 1 cut(s) 214
BsmAI GTCTC 1 cut(s) 929
BsmFI GGGAC 1 cut(s) 214
Bsp119I TTCGAA 1 cut(s) 1006
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 6 cut(s) 22, 157, 450, 628, 698, 975
BspACI CCGC 3 cut(s) 42, 250, 779
BspCNI CTCAG 1 cut(s) 895
BspMI ACCTGC 1 cut(s) 725
BspPI GGATC 2 cut(s) 30, 983
BspT104I TTCGAA 1 cut(s) 1006
BsrDI GCAATG 1 cut(s) 685
BsrI ACTGG 2 cut(s) 24, 282
BssECI CCNNGG 2 cut(s) 785, 879
BssMI GATC 6 cut(s) 22, 157, 450, 628, 698, 975
BssT1I CCWWGG 2 cut(s) 785, 879
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 2 cut(s) 422, 588
BstAPI GCANNNNNTGC 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 1006
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 1 cut(s) 903
BstF5I GGATG 4 cut(s) 112, 191, 313, 320
BstKTI GATC 6 cut(s) 25, 160, 453, 631, 701, 978
BstMAI GTCTC 1 cut(s) 929
BstMBI GATC 6 cut(s) 22, 157, 450, 628, 698, 975
BstMWI GCNNNNNNNGC 4 cut(s) 55, 256, 509, 518
BstNI CCWGG 1 cut(s) 582
BstNSI RCATGY 2 cut(s) 478, 528
BstSCI CCNGG 1 cut(s) 580
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 2 cut(s) 22, 975
BstYI RGATCY 2 cut(s) 22, 975
Bsu36I CCTNAGG 1 cut(s) 903
BtsCI GGATG 4 cut(s) 112, 191, 313, 320
BtsIMutI CAGTG 3 cut(s) 17, 361, 593
BveI ACCTGC 1 cut(s) 725
Cac8I GCNNGC 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 991
CviAII CATG 7 cut(s) 139, 254, 454, 475, 525, 530, 641
CviQI GTAC 1 cut(s) 991
DdeI CTNAG 1 cut(s) 903
DpnI GATC 6 cut(s) 24, 159, 452, 630, 700, 977
DpnII GATC 6 cut(s) 22, 157, 450, 628, 698, 975
Eco130I CCWWGG 2 cut(s) 785, 879
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 903
EcoRII CCWGG 1 cut(s) 580
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 2 cut(s) 785, 879
EcoT22I ATGCAT 1 cut(s) 1021
ErhI CCWWGG 2 cut(s) 785, 879
FaeI CATG 7 cut(s) 142, 257, 457, 478, 528, 533, 644
FalI AAGNNNNNCTT 4 cut(s) 83, 115, 657, 689
FaqI GGGAC 1 cut(s) 214
FatI CATG 7 cut(s) 138, 253, 453, 474, 524, 529, 640
FauI CCCGC 1 cut(s) 35
FbaI TGATCA 1 cut(s) 450
Fnu4HI GCNGC 2 cut(s) 251, 597
FokI GGATG 4 cut(s) 119, 198, 320, 327
Fsp4HI GCNGC 2 cut(s) 251, 597
FspBI CTAG 5 cut(s) 348, 402, 800, 861, 932
GluI GCNGC 2 cut(s) 251, 597
GsuI CTGGAG 1 cut(s) 33
HapII CCGG 1 cut(s) 832
Hin1II CATG 7 cut(s) 142, 257, 457, 478, 528, 533, 644
HindIII AAGCTT 2 cut(s) 47, 169
HinfI GANTC 4 cut(s) 194, 227, 351, 620
HpaII CCGG 1 cut(s) 832
HphI GGTGA 3 cut(s) 203, 424, 768
Hpy166II GTNNAC 4 cut(s) 79, 145, 329, 776
Hpy188I TCNGA 1 cut(s) 698
Hpy188III TCNNGA 3 cut(s) 12, 485, 689
Hpy8I GTNNAC 4 cut(s) 79, 145, 329, 776
HpyAV CCTTC 3 cut(s) 208, 953, 960
HpyCH4III ACNGT 2 cut(s) 422, 588
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 9 cut(s) 209, 274, 457, 503, 512, 533, 612, 734, 1019
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 256, 509, 518
HpyF3I CTNAG 1 cut(s) 903
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 7 cut(s) 142, 257, 457, 478, 528, 533, 644
Ksp22I TGATCA 1 cut(s) 450
Kzo9I GATC 6 cut(s) 22, 157, 450, 628, 698, 975
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 4 cut(s) 252, 371, 490, 1006
MaeI CTAG 5 cut(s) 348, 402, 800, 861, 932
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 4 cut(s) 163, 367, 416, 809
MalI GATC 6 cut(s) 24, 159, 452, 630, 700, 977
MboI GATC 6 cut(s) 22, 157, 450, 628, 698, 975
MboII GAAGA 3 cut(s) 125, 970, 988
MflI RGATCY 2 cut(s) 22, 975
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 3 cut(s) 342, 432, 654
MlyI GAGTC 3 cut(s) 203, 236, 614
MnlI CCTC 3 cut(s) 103, 736, 898
Mph1103I ATGCAT 1 cut(s) 1021
MseI TTAA 2 cut(s) 446, 657
MslI CAYNNNNRTG 3 cut(s) 84, 214, 986
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 1 cut(s) 832
MspR9I CCNGG 1 cut(s) 582
MvaI CCWGG 1 cut(s) 582
MwoI GCNNNNNNNGC 4 cut(s) 55, 256, 509, 518
NdeII GATC 6 cut(s) 22, 157, 450, 628, 698, 975
NlaIII CATG 7 cut(s) 142, 257, 457, 478, 528, 533, 644
NmuCI GTSAC 3 cut(s) 163, 416, 809
NsiI ATGCAT 1 cut(s) 1021
NspI RCATGY 2 cut(s) 478, 528
NspV TTCGAA 1 cut(s) 1006
PfeI GAWTC 1 cut(s) 351
PkrI GCNGC 2 cut(s) 252, 598
PleI GAGTC 3 cut(s) 202, 235, 614
PpsI GAGTC 3 cut(s) 202, 235, 614
Psp6I CCWGG 1 cut(s) 580
PspGI CCWGG 1 cut(s) 580
PspPI GGNCC 1 cut(s) 329
PsuI RGATCY 2 cut(s) 22, 975
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 992
RsaNI GTAC 1 cut(s) 991
RseI CAYNNNNRTG 3 cut(s) 84, 214, 986
SaqAI TTAA 2 cut(s) 446, 657
SatI GCNGC 2 cut(s) 251, 597
Sau3AI GATC 6 cut(s) 22, 157, 450, 628, 698, 975
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 3 cut(s) 203, 236, 614
ScrFI CCNGG 1 cut(s) 582
SduI GDGCHC 1 cut(s) 19
SfaNI GCATC 4 cut(s) 252, 371, 490, 1006
SfuI TTCGAA 1 cut(s) 1006
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 3 cut(s) 84, 214, 986
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 3 cut(s) 342, 432, 654
SsiI CCGC 3 cut(s) 42, 250, 779
SspI AATATT 1 cut(s) 507
SspMI CTAG 5 cut(s) 348, 402, 800, 861, 932
StyD4I CCNGG 1 cut(s) 580
StyI CCWWGG 2 cut(s) 785, 879
TaaI ACNGT 2 cut(s) 422, 588
TaiI ACGT 1 cut(s) 446
TaqI TCGA 3 cut(s) 568, 631, 1006
TasI AATT 3 cut(s) 342, 432, 654
TatI WGTACW 1 cut(s) 990
TauI GCSGC 1 cut(s) 253
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 2 cut(s) 446, 657
Tru9I TTAA 2 cut(s) 446, 657
TscAI CASTG 3 cut(s) 24, 361, 593
TseFI GTSAC 3 cut(s) 163, 416, 809
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 3 cut(s) 163, 416, 809
TspDTI ATGAA 5 cut(s) 192, 485, 657, 807, 844
TspRI CASTG 3 cut(s) 24, 361, 593
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 1 cut(s) 432
XceI RCATGY 2 cut(s) 478, 528
XspI CTAG 5 cut(s) 348, 402, 800, 861, 932
Zsp2I ATGCAT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.