RchiOBHm_Chr3g0482141
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6 SMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003004
Physical Location & Seq
Forward (+)
28211537 .. 28214474
2938 bp
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UTR
Exon/CDS
Intron
PRQ44704

Sequence Viewer

Length: 627 bp
ATGATACTGAAAATAGAACTCAATCACCTTGCAGGAGTGGATGAGACCTGCAACTTTGTCAAGGCAGACTTTATGAACATGCCATTTCCTGATAACACTTTTGATGCAATATTTGCAATAGAAGCCACATGCCATGCACCAAATGTGTTTGATTGCTACAAAGAAATATATAGAGTGTTGAAGCCAGGACAGTGTTTTGCTGCTTACGAGTGGTGCATTACTGACTCCTTTGATCGAACAAACCATGAACACCAGAGAATTAAGGGGGAAGTTGAGCTTGGCAATGGACTTCCTGATATCAGATCAAAAGAACAACGTATTCACGCTCTAAATGTTGCAGGTTTTGAGGTTATATGGGAAAATGATGTTTCTGTGGGTTCACCGCTTCCTTGGTATATGCCACTAGATAAAAGTCACATTTCATTGAGTAGCTTCCGGCTTACAGCTTTGGGGCGTTTCATTACTAGAACAATGGTCAAAGCCTTAGAGTTTTTACGCCTTGCCCCTGAGGGTAGCCAAAGATTTCAGTCTTTCCTAGAGACAACAGCAGAAGCTCTTGTTGAAGGTGGAAGGAAAGGGATCTTCACACCAATGTACTTCTTCTTGGTTCGAAAGCCTGATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130
Pfam Domains
Protein Families
Methyltransf_11 Sterol_MT_C

Protein Analysis

208

Amino Acids

23.75

Weight (kDa)

5.7

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_25 PF13649 13 - 63 3.6e-09 Methyltransferase domain
Methyltransf_11 PF08241 15 - 65 2.3e-13 Methyltransferase domain
Methyltransf_31 PF13847 16 - 86 2.6e-07 Methyltransferase domain
Sterol_MT_C PF08498 143 - 206 6.4e-28 Sterol methyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 56, 329
AciI CCGC 1 cut(s) 383
AclWI GGATC 1 cut(s) 587
AfaI GTAC 1 cut(s) 596
AgsI TTSAA 2 cut(s) 181, 563
AjnI CCWGG 1 cut(s) 184
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
AluBI AGCT 4 cut(s) 277, 432, 446, 554
AluI AGCT 4 cut(s) 277, 432, 446, 554
Alw26I GTCTC 2 cut(s) 38, 533
AlwI GGATC 1 cut(s) 587
ApeKI GCWGC 1 cut(s) 200
AsuHPI GGTGA 2 cut(s) 17, 372
AsuII TTCGAA 1 cut(s) 610
AxyI CCTNAGG 1 cut(s) 507
BbvI GCAGC 1 cut(s) 187
BciT130I CCWGG 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 38, 533
BfaI CTAG 3 cut(s) 404, 465, 536
BfuAI ACCTGC 2 cut(s) 56, 329
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 1 cut(s) 186
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 2 cut(s) 94, 610
Bpu14I TTCGAA 1 cut(s) 610
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 389
Bse21I CCTNAGG 1 cut(s) 507
Bse3DI GCAATG 1 cut(s) 289
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 389
BseGI GGATG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 498
BseXI GCAGC 1 cut(s) 187
BsiSI CCGG 1 cut(s) 436
BsmAI GTCTC 2 cut(s) 38, 533
Bso31I GGTCTC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 610
Bsp143I GATC 3 cut(s) 232, 302, 579
BspACI CCGC 1 cut(s) 383
BspCNI CTCAG 1 cut(s) 499
BspMI ACCTGC 2 cut(s) 56, 329
BspPI GGATC 1 cut(s) 587
BspT104I TTCGAA 1 cut(s) 610
BspTNI GGTCTC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 389
BssMI GATC 3 cut(s) 232, 302, 579
BssT1I CCWWGG 1 cut(s) 389
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 192
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 610
BstDEI CTNAG 2 cut(s) 484, 507
BstF5I GGATG 1 cut(s) 46
BstKTI GATC 3 cut(s) 235, 305, 582
BstMAI GTCTC 2 cut(s) 38, 533
BstMBI GATC 3 cut(s) 232, 302, 579
BstMWI GCNNNNNNNGC 2 cut(s) 113, 122
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 2 cut(s) 82, 132
BstSCI CCNGG 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
Bsu36I CCTNAGG 1 cut(s) 507
BtsCI GGATG 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 197
BveI ACCTGC 2 cut(s) 56, 329
Csp6I GTAC 1 cut(s) 595
CviAII CATG 4 cut(s) 79, 129, 134, 245
CviQI GTAC 1 cut(s) 595
DdeI CTNAG 2 cut(s) 484, 507
DpnI GATC 3 cut(s) 234, 304, 581
DpnII GATC 3 cut(s) 232, 302, 579
Eco130I CCWWGG 1 cut(s) 389
Eco31I GGTCTC 1 cut(s) 38
Eco32I GATATC 1 cut(s) 298
Eco81I CCTNAGG 1 cut(s) 507
EcoRII CCWGG 1 cut(s) 184
EcoRV GATATC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 389
EcoT22I ATGCAT 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 389
FaeI CATG 4 cut(s) 82, 132, 137, 248
FalI AAGNNNNNCTT 4 cut(s) 53, 85, 261, 293
FatI CATG 4 cut(s) 78, 128, 133, 244
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 3 cut(s) 404, 465, 536
GluI GCNGC 1 cut(s) 201
HapII CCGG 1 cut(s) 436
Hin1II CATG 4 cut(s) 82, 132, 137, 248
HinfI GANTC 1 cut(s) 224
HpaII CCGG 1 cut(s) 436
HphI GGTGA 2 cut(s) 17, 372
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 1 cut(s) 302
Hpy188III TCNNGA 2 cut(s) 89, 293
Hpy8I GTNNAC 1 cut(s) 380
HpyAV CCTTC 2 cut(s) 557, 564
HpyCH4III ACNGT 1 cut(s) 192
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 8 cut(s) 32, 51, 107, 116, 137, 216, 338, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 122
HpyF3I CTNAG 2 cut(s) 484, 507
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 4 cut(s) 82, 132, 137, 248
Kzo9I GATC 3 cut(s) 232, 302, 579
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 2 cut(s) 94, 610
MaeI CTAG 3 cut(s) 404, 465, 536
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 1 cut(s) 413
MalI GATC 3 cut(s) 234, 304, 581
MboI GATC 3 cut(s) 232, 302, 579
MboII GAAGA 2 cut(s) 574, 592
MflI RGATCY 1 cut(s) 579
MluCI AATT 1 cut(s) 258
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 2 cut(s) 340, 502
Mph1103I ATGCAT 1 cut(s) 625
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 1 cut(s) 590
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 113, 122
NdeII GATC 3 cut(s) 232, 302, 579
NlaIII CATG 4 cut(s) 82, 132, 137, 248
NmuCI GTSAC 1 cut(s) 413
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 2 cut(s) 82, 132
NspV TTCGAA 1 cut(s) 610
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 579
RsaI GTAC 1 cut(s) 596
RsaNI GTAC 1 cut(s) 595
RseI CAYNNNNRTG 1 cut(s) 590
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 3 cut(s) 232, 302, 579
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 186
SfaNI GCATC 2 cut(s) 94, 610
SfuI TTCGAA 1 cut(s) 610
SmiMI CAYNNNNRTG 1 cut(s) 590
Sse9I AATT 1 cut(s) 258
SsiI CCGC 1 cut(s) 383
SspI AATATT 1 cut(s) 111
SspMI CTAG 3 cut(s) 404, 465, 536
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 389
TaaI ACNGT 1 cut(s) 192
TaiI ACGT 1 cut(s) 319
TaqI TCGA 2 cut(s) 235, 610
TasI AATT 1 cut(s) 258
TatI WGTACW 1 cut(s) 594
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 1 cut(s) 197
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 4 cut(s) 89, 261, 411, 448
TspRI CASTG 1 cut(s) 197
XceI RCATGY 2 cut(s) 82, 132
XspI CTAG 3 cut(s) 404, 465, 536
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.