Rh1CG024000
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6 SMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003004
Physical Location & Seq
Forward (+)
4476445 .. 4482386
5942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG024000.1

Sequence Viewer

Length: 909 bp
ATGGTTAATAAATATTATGATCTGGTTACCAGCTTTTATGAGTATGGCTGGGGAGAGTCCTTCCATTTTGCACCCAGATGGAAAGGAGAGTCTCTTCGAGAGAGCATCAAGAGGCATGAACACTTCCTTGCCTTACAACTAGGACTGAAACCTGGACAGAAGGTTTTGGATGTAGGTTGTGGAATTGGAGGACCACTTAGAGAAATAGCACGCTTCAGCTCGACGTCAATTACTGGGTTGAACAACAATGAATATCAGATAACAAGAGGAAAGGAACTGAACCGCATTGTTGGAATTGACAAGACCTGTGACTTTGTTAAGGCCGACTTCATGAAAATACCATTTCCTGAAAATACGTTTGATGCAGTATATGCAATCGAAGCTACCTGCCATGCACCAGATGCATATGGATGTTACAAAGAGATTTATAGAGTACTAAAACCTGGTCAATGTTTTGCTGCATATGAGTGGTGCATGACCGATTCGTTTGATCCCAATAACGCAGAACATCAAAAAATTAAGGCAGAGATTGAGATTGGTGATGGCCTCCCGGACATCAGATTGACAGTAAAGTGCCTTGAAGCTTTGAAACAAGCAGGTTTTGAGGTCATATGGGAGAAAGATCTTGCAAAGGACGTAAAGGAGTCACCTCTTCCTTGGTACTTGCCTTTGGACACAAGTCGCATTTCACTTAGTAGTTTCCGTCTAACAGCTGTTGGCCGCTTTTTTACTAGAAACATGGTCAAAGCTTTAGAATATGTTGGACTTGCTCCACAAGGAAGCCAAAGAGTTCAAGATTTTCTAGAGAAAGCTGCTGAAGGGCTAGTCGAAGGTGGAAAGAAAGAGATCTTCACACCATTGTATTTCTTTGTGGCCCGGAAGCCACTTTCAGCTGCAGATAGTCAGTAA

Protein Analysis

302

Amino Acids

34.18

Weight (kDa)

6.26

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 2 - 220 4.6e-10 Mycolic acid cyclopropane synthetase
Ubie_methyltran PF01209 42 - 157 9.2e-10 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 43 - 150 2.9e-08 Methyltransferase domain
Methyltransf_31 PF13847 50 - 170 5e-15 Methyltransferase domain
Methyltransf_25 PF13649 55 - 149 1.6e-17 Methyltransferase domain
Methyltransf_11 PF08241 56 - 151 9.5e-21 Methyltransferase domain
Methyltransf_12 PF08242 56 - 149 2.4e-08 Methyltransferase domain
Sterol_MT_C PF08498 232 - 295 3.6e-29 Sterol methyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 227
Acc36I ACCTGC 2 cut(s) 395, 587
AciI CCGC 2 cut(s) 283, 721
AclWI GGATC 1 cut(s) 485
AcoI YGGCCR 1 cut(s) 718
AcuI CTGAAG 2 cut(s) 199, 837
AcyI GRCGYC 1 cut(s) 224
AfaI GTAC 2 cut(s) 435, 662
AgsI TTSAA 4 cut(s) 241, 581, 589, 794
AjnI CCWGG 2 cut(s) 151, 442
AluBI AGCT 8 cut(s) 33, 219, 383, 584, 713, 749, 812, 893
AluI AGCT 8 cut(s) 33, 219, 383, 584, 713, 749, 812, 893
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 1 cut(s) 485
AoxI GGCC 4 cut(s) 321, 544, 718, 873
ApeKI GCWGC 3 cut(s) 458, 812, 893
AspS9I GGNCC 2 cut(s) 191, 874
AsuC2I CCSGG 2 cut(s) 551, 877
AsuHPI GGTGA 2 cut(s) 551, 639
AvaII GGWCC 1 cut(s) 191
BbvI GCAGC 3 cut(s) 445, 799, 880
BccI CCATC 2 cut(s) 72, 536
BciT130I CCWGG 2 cut(s) 153, 444
BcnI CCSGG 2 cut(s) 551, 877
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 4 cut(s) 140, 732, 803, 824
BfmI CTRYAG 1 cut(s) 894
BfuAI ACCTGC 2 cut(s) 395, 587
BglII AGATCT 2 cut(s) 622, 846
BisI GCNGC 4 cut(s) 459, 721, 813, 894
BlsI GCNGC 4 cut(s) 460, 722, 814, 895
BmcAI AGTACT 1 cut(s) 435
Bme1390I CCNGG 4 cut(s) 153, 444, 551, 877
Bme18I GGWCC 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 191, 874
BmrFI CCNGG 4 cut(s) 153, 444, 551, 877
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 3 cut(s) 114, 352, 391
BmuI ACTGGG 1 cut(s) 243
BoxI GACNNNNGTC 1 cut(s) 678
BpuMI CCSGG 2 cut(s) 551, 877
BsaHI GRCGYC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 656
Bse1I ACTGG 1 cut(s) 238
BseBI CCWGG 2 cut(s) 153, 444
BseDI CCNNGG 1 cut(s) 656
BseGI GGATG 2 cut(s) 175, 416
BseNI ACTGG 1 cut(s) 238
BseXI GCAGC 3 cut(s) 445, 799, 880
BseYI CCCAGC 1 cut(s) 48
BshFI GGCC 4 cut(s) 323, 546, 720, 875
BsiSI CCGG 2 cut(s) 551, 877
BsmAI GTCTC 1 cut(s) 96
BsnI GGCC 4 cut(s) 323, 546, 720, 875
Bsp143I GATC 4 cut(s) 19, 490, 622, 846
BspACI CCGC 2 cut(s) 283, 721
BspANI GGCC 4 cut(s) 323, 546, 720, 875
BspHI TCATGA 1 cut(s) 330
BspMAI CTGCAG 1 cut(s) 898
BspMI ACCTGC 2 cut(s) 395, 587
BspPI GGATC 1 cut(s) 485
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 656
BssMI GATC 4 cut(s) 19, 490, 622, 846
BssNI GRCGYC 1 cut(s) 224
BssT1I CCWWGG 1 cut(s) 656
Bst2UI CCWGG 2 cut(s) 153, 444
Bst4CI ACNGT 1 cut(s) 568
Bst6I CTCTTC 2 cut(s) 99, 657
BstACI GRCGYC 1 cut(s) 224
BstAPI GCANNNNNTGC 2 cut(s) 371, 401
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 197, 692
BstEII GGTNACC 1 cut(s) 25
BstF5I GGATG 2 cut(s) 175, 416
BstKTI GATC 4 cut(s) 22, 493, 625, 849
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 4 cut(s) 19, 490, 622, 846
BstMWI GCNNNNNNNGC 3 cut(s) 371, 380, 401
BstNI CCWGG 2 cut(s) 153, 444
BstPAI GACNNNNGTC 1 cut(s) 678
BstPI GGTNACC 1 cut(s) 25
BstSCI CCNGG 4 cut(s) 151, 442, 549, 875
BstSFI CTRYAG 1 cut(s) 894
BstV1I GCAGC 3 cut(s) 445, 799, 880
BstX2I RGATCY 2 cut(s) 622, 846
BstYI RGATCY 2 cut(s) 622, 846
BsuRI GGCC 4 cut(s) 323, 546, 720, 875
BtsCI GGATG 2 cut(s) 175, 416
BveI ACCTGC 2 cut(s) 395, 587
Cac8I GCNNGC 1 cut(s) 211
CciI TCATGA 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 191, 874
CsiI ACCWGGT 1 cut(s) 442
Csp6I GTAC 2 cut(s) 434, 661
CviAII CATG 5 cut(s) 116, 331, 392, 475, 739
CviQI GTAC 2 cut(s) 434, 661
DdeI CTNAG 2 cut(s) 197, 692
DpnI GATC 4 cut(s) 21, 492, 624, 848
DpnII GATC 4 cut(s) 19, 490, 622, 846
EaeI YGGCCR 1 cut(s) 718
Eam1104I CTCTTC 2 cut(s) 99, 657
EarI CTCTTC 2 cut(s) 99, 657
Eco130I CCWWGG 1 cut(s) 656
Eco47I GGWCC 1 cut(s) 191
Eco57I CTGAAG 2 cut(s) 199, 837
Eco91I GGTNACC 1 cut(s) 25
EcoO65I GGTNACC 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 151, 442
EcoT14I CCWWGG 1 cut(s) 656
EcoT22I ATGCAT 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 656
FaeI CATG 5 cut(s) 119, 334, 395, 478, 742
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FatI CATG 5 cut(s) 115, 330, 391, 474, 738
FauNDI CATATG 3 cut(s) 406, 463, 611
Fnu4HI GCNGC 4 cut(s) 459, 721, 813, 894
FokI GGATG 2 cut(s) 182, 423
Fsp4HI GCNGC 4 cut(s) 459, 721, 813, 894
FspBI CTAG 4 cut(s) 140, 732, 803, 824
GluI GCNGC 4 cut(s) 459, 721, 813, 894
GsaI CCCAGC 1 cut(s) 52
HaeIII GGCC 4 cut(s) 323, 546, 720, 875
HapII CCGG 2 cut(s) 551, 877
Hin1I GRCGYC 1 cut(s) 224
Hin1II CATG 5 cut(s) 119, 334, 395, 478, 742
HindIII AAGCTT 2 cut(s) 582, 747
HinfI GANTC 4 cut(s) 56, 89, 482, 644
HpaII CCGG 2 cut(s) 551, 877
HphI GGTGA 2 cut(s) 551, 639
Hpy188I TCNGA 2 cut(s) 258, 560
Hpy188III TCNNGA 6 cut(s) 98, 109, 331, 347, 794, 803
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 4 cut(s) 70, 154, 812, 824
HpyCH4III ACNGT 1 cut(s) 568
HpyCH4IV ACGT 3 cut(s) 224, 356, 636
HpyCH4V TGCA 9 cut(s) 71, 365, 374, 395, 404, 461, 474, 629, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 380, 401
HpyF3I CTNAG 2 cut(s) 197, 692
HpySE526I ACGT 3 cut(s) 224, 356, 636
Hsp92I GRCGYC 1 cut(s) 224
Hsp92II CATG 5 cut(s) 119, 334, 395, 478, 742
Kzo9I GATC 4 cut(s) 19, 490, 622, 846
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 3 cut(s) 445, 799, 880
LweI GCATC 3 cut(s) 114, 352, 391
MabI ACCWGGT 1 cut(s) 442
MaeI CTAG 4 cut(s) 140, 732, 803, 824
MaeII ACGT 3 cut(s) 224, 356, 636
MaeIII GTNAC 4 cut(s) 25, 308, 413, 645
MalI GATC 4 cut(s) 21, 492, 624, 848
MboI GATC 4 cut(s) 19, 490, 622, 846
MboII GAAGA 3 cut(s) 86, 644, 841
MflI RGATCY 2 cut(s) 622, 846
MluCI AATT 4 cut(s) 183, 228, 294, 516
MlyI GAGTC 3 cut(s) 65, 98, 653
MmeI TCCRAC 2 cut(s) 271, 742
MnlI CCTC 6 cut(s) 105, 182, 260, 557, 598, 660
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 3 cut(s) 6, 318, 519
MslI CAYNNNNRTG 3 cut(s) 76, 409, 466
MspA1I CMGCKG 2 cut(s) 713, 893
MspI CCGG 2 cut(s) 551, 877
MspR9I CCNGG 4 cut(s) 153, 444, 551, 877
MvaI CCWGG 2 cut(s) 153, 444
MwoI GCNNNNNNNGC 3 cut(s) 371, 380, 401
NciI CCSGG 2 cut(s) 551, 877
NdeI CATATG 3 cut(s) 406, 463, 611
NdeII GATC 4 cut(s) 19, 490, 622, 846
NlaIII CATG 5 cut(s) 119, 334, 395, 478, 742
NmuCI GTSAC 2 cut(s) 308, 645
NsiI ATGCAT 1 cut(s) 406
PagI TCATGA 1 cut(s) 330
PfeI GAWTC 1 cut(s) 482
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 4 cut(s) 460, 722, 814, 895
PleI GAGTC 3 cut(s) 64, 97, 652
PpsI GAGTC 3 cut(s) 64, 97, 652
PshAI GACNNNNGTC 1 cut(s) 678
Psp6I CCWGG 2 cut(s) 151, 442
PspEI GGTNACC 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 48
PspGI CCWGG 2 cut(s) 151, 442
PspPI GGNCC 2 cut(s) 191, 874
PstI CTGCAG 1 cut(s) 898
PsuI RGATCY 2 cut(s) 622, 846
PvuII CAGCTG 2 cut(s) 713, 893
RsaI GTAC 2 cut(s) 435, 662
RsaNI GTAC 2 cut(s) 434, 661
RseI CAYNNNNRTG 3 cut(s) 76, 409, 466
SaqAI TTAA 3 cut(s) 6, 318, 519
SatI GCNGC 4 cut(s) 459, 721, 813, 894
Sau3AI GATC 4 cut(s) 19, 490, 622, 846
Sau96I GGNCC 2 cut(s) 191, 874
ScaI AGTACT 1 cut(s) 435
SchI GAGTC 3 cut(s) 65, 98, 653
ScrFI CCNGG 4 cut(s) 153, 444, 551, 877
SexAI ACCWGGT 1 cut(s) 442
SfaNI GCATC 3 cut(s) 114, 352, 391
SfcI CTRYAG 1 cut(s) 894
SinI GGWCC 1 cut(s) 191
SmiMI CAYNNNNRTG 3 cut(s) 76, 409, 466
Sse9I AATT 4 cut(s) 183, 228, 294, 516
SsiI CCGC 2 cut(s) 283, 721
SspI AATATT 1 cut(s) 14
SspMI CTAG 4 cut(s) 140, 732, 803, 824
StyD4I CCNGG 4 cut(s) 151, 442, 549, 875
StyI CCWWGG 1 cut(s) 656
TaaI ACNGT 1 cut(s) 568
TaiI ACGT 3 cut(s) 227, 359, 639
TaqI TCGA 4 cut(s) 97, 221, 378, 828
TaqII GACCGA 1 cut(s) 494
TasI AATT 4 cut(s) 183, 228, 294, 516
TatI WGTACW 1 cut(s) 433
TauI GCSGC 1 cut(s) 723
TfiI GAWTC 1 cut(s) 482
Tru1I TTAA 3 cut(s) 6, 318, 519
Tru9I TTAA 3 cut(s) 6, 318, 519
TseFI GTSAC 2 cut(s) 308, 645
TseI GCWGC 3 cut(s) 458, 812, 893
Tsp45I GTSAC 2 cut(s) 308, 645
TspDTI ATGAA 4 cut(s) 132, 264, 319, 347
TspGWI ACGGA 1 cut(s) 692
VpaK11BI GGWCC 1 cut(s) 191
XbaI TCTAGA 1 cut(s) 802
XspI CTAG 4 cut(s) 140, 732, 803, 824
ZraI GACGTC 1 cut(s) 225
ZrmI AGTACT 1 cut(s) 435
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.