Rh3DG273600
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6 SMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0003004
Physical Location & Seq
Forward (+)
27541146 .. 27546221
5076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG273600.1

Sequence Viewer

Length: 972 bp
ATGTCAAAATCTGGAGCACTGGATCTGGCTTCTGGGGTTGGCGGGAAGCTTGAGAAGCACCAAGTTCTTTCAGCTGTCCACAAATATGAGAAGTATCACTTTGGGGATGAGGAAGACAGAAAAGCCAACTATACTGACATGGTTAACAAATACTATGATCTCGTCACAAGCTTTTATGAATATGGCTGGGGTGAGTCCTTCCATTTTGCACCAAGATGGAAGCAAGAGTCCCTAAAAGAGAGCATCAAGCGGCATGAGCATTTTATCGCTCTGCAACTGGGTCTAAAGCAAGGGCATATGGTTTTGGATGTAGGATGTGGGATTGGTGGACCTATGAGAGAAATTACTAGATTCAGTGGGGCATCAGTTGCAGGGATAAACAACAACGAATATCAAATAACTAGAGCAAAGGAACTCAATCACCTTGCAGGAGTGGATGAGACCTGCAACTTTGTCAAGGCAGACTTTATGAACATGCCATTTCCTGATAACACTTTTGATGCAATATTTGCAATAGAAGCCACATGCCATGCACCAAATGTGTTTGATTGCTACAAAGAAATATATAGAGTGTTGAAGCCAGGACAGTGTTTTGCTGCTTACGAGTGGTGCATTACTGATTCCTTTGATCGAACAAACTATGAACACCAGCGAATTAAGGGGGAAGTTGAGCTTGGCAATGGACTTCCTGATATCAGATCAAAAGAACAATGTATTCACGCTCTAAATGTTGCAGGTTTTGAGGTTATATGGGAAAATGATGTTTCTGTGGGTTCACCGCTTCCTTGGTATATGCCACTAGATAAAAGTCACATTTCATTGAGTAGCTTCCGGCTTACAGCTTTGGGGCGTTTCATTACTAGAACAATGGTCAAAGCCTTAGAGTTTTTACGCCTTGCCCCTGAGGGTAGCCAAAGAGTTCAGTCTTTCCTAGAGACAACAGCAGAAGCTCTTGTTGAAGGTGGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130
Pfam Domains
Protein Families
Methyltransf_11 Sterol_MT_C

Protein Analysis

323

Amino Acids

36.57

Weight (kDa)

5.86

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 42 - 212 1e-11 Mycolic acid cyclopropane synthetase
Ubie_methyltran PF01209 87 - 202 8.3e-09 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 91 - 249 1.5e-08 Methyltransferase domain
Methyltransf_31 PF13847 96 - 214 5.8e-16 Methyltransferase domain
Methyltransf_25 PF13649 101 - 195 1.4e-18 Methyltransferase domain
Methyltransf_11 PF08241 102 - 197 5.7e-22 Methyltransferase domain
Methyltransf_12 PF08242 102 - 195 9.1e-09 Methyltransferase domain
Sterol_MT_C PF08498 275 - 323 8.9e-16 Sterol methyltransferase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 452, 725
AciI CCGC 3 cut(s) 42, 250, 779
AclWI GGATC 1 cut(s) 30
AgsI TTSAA 2 cut(s) 577, 959
AjnI CCWGG 1 cut(s) 580
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AluBI AGCT 7 cut(s) 49, 74, 171, 673, 828, 842, 950
AluI AGCT 7 cut(s) 49, 74, 171, 673, 828, 842, 950
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 434, 929
AlwI GGATC 1 cut(s) 30
ApeKI GCWGC 1 cut(s) 596
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 3 cut(s) 203, 413, 768
AvaII GGWCC 1 cut(s) 329
AxyI CCTNAGG 1 cut(s) 903
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 583
BccI CCATC 1 cut(s) 210
BciT130I CCWGG 1 cut(s) 582
BcoDI GTCTC 2 cut(s) 434, 929
BfaI CTAG 5 cut(s) 348, 402, 800, 861, 932
BfuAI ACCTGC 2 cut(s) 452, 725
BisI GCNGC 2 cut(s) 251, 597
BlsI GCNGC 2 cut(s) 252, 598
Bme1390I CCNGG 1 cut(s) 582
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmrFI CCNGG 1 cut(s) 582
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 3 cut(s) 252, 371, 490
BmuI ACTGGG 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 1 cut(s) 785
Bse1I ACTGG 2 cut(s) 24, 282
Bse21I CCTNAGG 1 cut(s) 903
Bse3DI GCAATG 1 cut(s) 685
BseBI CCWGG 1 cut(s) 582
BseDI CCNNGG 1 cut(s) 785
BseGI GGATG 4 cut(s) 112, 313, 320, 442
BseMI GCAATG 1 cut(s) 685
BseMII CTCAG 1 cut(s) 894
BseNI ACTGG 2 cut(s) 24, 282
BseXI GCAGC 1 cut(s) 583
BseYI CCCAGC 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 1 cut(s) 832
BslFI GGGAC 1 cut(s) 214
BsmAI GTCTC 2 cut(s) 434, 929
BsmFI GGGAC 1 cut(s) 214
Bso31I GGTCTC 1 cut(s) 434
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 4 cut(s) 22, 157, 628, 698
BspACI CCGC 3 cut(s) 42, 250, 779
BspCNI CTCAG 1 cut(s) 895
BspMI ACCTGC 2 cut(s) 452, 725
BspPI GGATC 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 685
BsrI ACTGG 2 cut(s) 24, 282
BssECI CCNNGG 1 cut(s) 785
BssMI GATC 4 cut(s) 22, 157, 628, 698
BssT1I CCWWGG 1 cut(s) 785
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 1 cut(s) 588
BstAPI GCANNNNNTGC 2 cut(s) 368, 509
BstDEI CTNAG 2 cut(s) 880, 903
BstF5I GGATG 4 cut(s) 112, 313, 320, 442
BstKTI GATC 4 cut(s) 25, 160, 631, 701
BstMAI GTCTC 2 cut(s) 434, 929
BstMBI GATC 4 cut(s) 22, 157, 628, 698
BstMWI GCNNNNNNNGC 5 cut(s) 55, 256, 368, 509, 518
BstNI CCWGG 1 cut(s) 582
BstNSI RCATGY 2 cut(s) 478, 528
BstSCI CCNGG 1 cut(s) 580
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
Bsu36I CCTNAGG 1 cut(s) 903
BtsCI GGATG 4 cut(s) 112, 313, 320, 442
BtsIMutI CAGTG 3 cut(s) 17, 361, 593
BveI ACCTGC 2 cut(s) 452, 725
Cfr13I GGNCC 1 cut(s) 329
CviAII CATG 5 cut(s) 139, 254, 475, 525, 530
DdeI CTNAG 2 cut(s) 880, 903
DpnI GATC 4 cut(s) 24, 159, 630, 700
DpnII GATC 4 cut(s) 22, 157, 628, 698
Eco130I CCWWGG 1 cut(s) 785
Eco31I GGTCTC 1 cut(s) 434
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 903
EcoRII CCWGG 1 cut(s) 580
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 1 cut(s) 785
ErhI CCWWGG 1 cut(s) 785
FaeI CATG 5 cut(s) 142, 257, 478, 528, 533
FalI AAGNNNNNCTT 6 cut(s) 83, 115, 449, 481, 657, 689
FaqI GGGAC 1 cut(s) 214
FatI CATG 5 cut(s) 138, 253, 474, 524, 529
FauI CCCGC 1 cut(s) 35
FauNDI CATATG 1 cut(s) 297
Fnu4HI GCNGC 2 cut(s) 251, 597
FokI GGATG 4 cut(s) 119, 320, 327, 449
Fsp4HI GCNGC 2 cut(s) 251, 597
FspBI CTAG 5 cut(s) 348, 402, 800, 861, 932
GluI GCNGC 2 cut(s) 251, 597
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 33
HapII CCGG 1 cut(s) 832
Hin1II CATG 5 cut(s) 142, 257, 478, 528, 533
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 2 cut(s) 47, 169
HinfI GANTC 4 cut(s) 194, 227, 351, 620
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 1 cut(s) 832
HphI GGTGA 3 cut(s) 203, 413, 768
Hpy166II GTNNAC 4 cut(s) 79, 145, 329, 776
Hpy188I TCNGA 1 cut(s) 698
Hpy188III TCNNGA 3 cut(s) 12, 485, 689
Hpy8I GTNNAC 4 cut(s) 79, 145, 329, 776
HpyAV CCTTC 3 cut(s) 208, 953, 960
HpyCH4III ACNGT 1 cut(s) 588
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 256, 368, 509, 518
HpyF3I CTNAG 2 cut(s) 880, 903
Hsp92II CATG 5 cut(s) 142, 257, 478, 528, 533
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 4 cut(s) 22, 157, 628, 698
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 3 cut(s) 252, 371, 490
MaeI CTAG 5 cut(s) 348, 402, 800, 861, 932
MaeIII GTNAC 2 cut(s) 163, 809
MalI GATC 4 cut(s) 24, 159, 630, 700
MboI GATC 4 cut(s) 22, 157, 628, 698
MboII GAAGA 1 cut(s) 125
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 2 cut(s) 342, 654
MlyI GAGTC 2 cut(s) 203, 236
MnlI CCTC 3 cut(s) 103, 736, 898
MseI TTAA 2 cut(s) 144, 657
MslI CAYNNNNRTG 2 cut(s) 84, 214
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 1 cut(s) 832
MspR9I CCNGG 1 cut(s) 582
MvaI CCWGG 1 cut(s) 582
MwoI GCNNNNNNNGC 5 cut(s) 55, 256, 368, 509, 518
NdeI CATATG 1 cut(s) 297
NdeII GATC 4 cut(s) 22, 157, 628, 698
NlaIII CATG 5 cut(s) 142, 257, 478, 528, 533
NmuCI GTSAC 2 cut(s) 163, 809
NspI RCATGY 2 cut(s) 478, 528
PfeI GAWTC 2 cut(s) 351, 620
PkrI GCNGC 2 cut(s) 252, 598
PleI GAGTC 2 cut(s) 202, 235
PpsI GAGTC 2 cut(s) 202, 235
Psp6I CCWGG 1 cut(s) 580
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 580
PspPI GGNCC 1 cut(s) 329
PsuI RGATCY 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 74
RseI CAYNNNNRTG 2 cut(s) 84, 214
SaqAI TTAA 2 cut(s) 144, 657
SatI GCNGC 2 cut(s) 251, 597
Sau3AI GATC 4 cut(s) 22, 157, 628, 698
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 2 cut(s) 203, 236
ScrFI CCNGG 1 cut(s) 582
SduI GDGCHC 1 cut(s) 19
SfaNI GCATC 3 cut(s) 252, 371, 490
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 84, 214
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 2 cut(s) 342, 654
SsiI CCGC 3 cut(s) 42, 250, 779
SspI AATATT 1 cut(s) 507
SspMI CTAG 5 cut(s) 348, 402, 800, 861, 932
StyD4I CCNGG 1 cut(s) 580
StyI CCWWGG 1 cut(s) 785
TaaI ACNGT 1 cut(s) 588
TaqI TCGA 1 cut(s) 631
TasI AATT 2 cut(s) 342, 654
TauI GCSGC 1 cut(s) 253
TfiI GAWTC 2 cut(s) 351, 620
Tru1I TTAA 2 cut(s) 144, 657
Tru9I TTAA 2 cut(s) 144, 657
TscAI CASTG 3 cut(s) 24, 361, 593
TseFI GTSAC 2 cut(s) 163, 809
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 2 cut(s) 163, 809
TspDTI ATGAA 5 cut(s) 192, 485, 657, 807, 844
TspRI CASTG 3 cut(s) 24, 361, 593
VpaK11BI GGWCC 1 cut(s) 329
XceI RCATGY 2 cut(s) 478, 528
XspI CTAG 5 cut(s) 348, 402, 800, 861, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.