Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007907

Physical Location & Seq

Genomic Coordinates Forward (+)

36880773 .. 36890372

Gene Structure 9600 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1313300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3819 bp

ATGGCAGCCATGGTGGCGGAAGAAAACCCAAAGCAGAAGCAGGAACTTGCAATGGGTAAAGAAGACGATGACGACGAGCATGGCGCCAAGGAGGCAGTGCTCCAAAAGTACTTCCTTCAAGAATGGAAGCTCGTCAAGTCCATCTTAAACGACATCGTCTCAAATGGGCGGGTGTCCGATCCCTCCGCTCCCCACAAGATCCGATCCATTATGGACAAGTATCAAGAACAGGGTCAACTAGTTGAACCTTACCTGGAAAGCATCGTTACCCCGCTCATGTTTATTGTTCGCTCTAAAACAGTAGAATTAGGTGTAGCTTCAGATGAAATTCTTGAAGTAATTAAACCCATATGCCTTATTATTTATTCTCTCGTCACCGTTTGTGGATACAAGGCGGTTGTCAGGTTCTTTCCGCATCAAGTTTCGGACTTGGAACTTGCTGTGTCTCTTTTGGAGAAGTGCCATCACACAAATTCAGTGTCATCACTGCGGCAGGAAAGTACAGGAGAGATGGAAGCTAAATGTGTGATGCTTTTGTGGCTGTCTATACTCGTGTTGGTTCCGTTTGATATCTCCACTGTTGATACTAGCATTGCAAACAACAGTAATCTTGGAAAACTTGAGCCAGCTCCACTGGTATTGAGGATAGTAGGGCTCTCCGAAGATTACCTTTCAAATTCAGGCCCTATGCGCCCTATTGCTGCTTTGCTGCTCTCGAAGCTTCTAACACGTCCAGACATGCCAAGGGCCTTTTCCAGCTTTGTTCAATGGTCACACGAAGTCCTATCTTCTCTGAAAAATGATGCCATCAATCATTTCCGGTTACTTGGAGCTACAGAAGCACTGGCTGCTATTTTCAAGGTTGGTGGGCGGAAACTCTTGCTTGATGTGGTTACTATTGTTTGGGTTGACACCTTATTGTTGATCAAGTCTAGCATTGCCGCTAGGAGCCCATTACTGCGCAAGCATCTGATGAAATTAACACAGAGGATTGGGCTTATTTGCCTCCCTCATCGTACACCTTCATGGTGCTATGTGGGGAAAACAAGCTCTTTGGGAGAGAACGTAACTTTGTCTGCATCTGAAAAAACTGGTCGATACAATTATGCTTTAGATGATGAGGTTTCCAACTCCGAACCAAGTTCAAGCTGCCAGCAAGATGAAGAAATGGATGTTCCAGAAGTCGTAGAAGAGATTATTGAGATGCTACTTACTGGGTTGAGAGACACGGACACGGTTGTGCGTTGGTCTGCTGCAAAAGGCATAGGCCGCATTACATCATGTCTTACATCTGCCCTTTCAGAGGAGGTCCTGTCATCTGTGTTGGAGCTGTTTTCACCAGGCGAGGGTGATGGGTCATGGCATGGTGGTTGCTTAGCACTGGCCGAATTGGCACGTAAAGGGTTGCTCTTACCTATCAGCCTTCCAAAAGTTGTACCCGTTGTTTTGAAGGCACTACATTATGATATTCGAAGAGGTCCACATAGTGTTGGATCTCATGTACGGGATGCTGCAGCATATGTTTGTTGGGCTTTTGGCCGTGCATATTATCACACGGATATGAGAAATATACTGGACCAGCTTGCGCCACACCTTCTGACAGTGGCCTGCTACGACCGTGAGGTTAATTGTAGAAGAGCAGCCGCTGCGGCTTTTCAAGAGAATGTTGGAAGACAGGGTAGTTACCCTCATGGAATTGACATAGTGAACACTGCGGACTATTTTTCACTTTCTTCACGAATAAACTCTTATGTTCATGTTGCTGTCTCTATTGCTCAATATGAGGATTATCTTTATCCATTTGTGGATGAGCTGCTGTATAACAAAATTTGTCACTGGGATAAAGGATTGAGAGAACTTGCGGCTGAGGCTCTTTCTGCCCTTGTTAAATATGATCCTGAATATCTTATAAACTATGCTGTGGAGAAAATAATTCCTTGCACTCTCTCATCTGATTTGTGCATGCGCCATGGAGCAACACTGGCAGCTGGGGAACTTGTTTTAGCACTGCACAAGTGTGGTTATGCTCTTTCTGCAGATAAGCAAAAACGTGTTGCTGGTGTTGTGCCTGCTATTGAGAAAGCACGCCTTTATCGTGGAAAAGGCGGAGAAATAATGCGTGCAGCTGTTTCCCGCTTCATTGAATGTGTATCTATATCATCTGTGTCACTGCCCGAAAAGATAAAATGTAGTCTGCTTGATGGTCTTAATGAGAATTTGAGACACCCTAATTCGCAAATTCAGGATGCTGCAGCTGAAGCTCTGAAGCATTTTGTGCCAACCTATCTTGTTGTTTCATCTGTTGGAGGTACTGGTGATATAACATCAAAGTACCTCGAACTCTTGAGTGACCCAAATGTGGCTATAAGAAGAGGGTCAGCACTAGCGGTAGGTGTTTTGCCTTGTGAACTTTTCGCCCATAGGTGGAAGGATGTGCTTCTGAAGCTTTGCAACTCTTGTTTAATTGAGGATAATCCTGATGATAGAGATGCTGAAGCACGAGTAAATGCTGTCAAAGGACTTGTGTCAGTGTGTGAAGCATTAACTCGGGAAAAAGAACAATCTGGTATTGATGCTGTGGAGGGTGACATGTCCTTGTTTCTTCTCATCAAGGATGAAATAATGATGACTTTATTAAAAGCTCTTGATGACTATTCTGTTGATAACCGAGGCGATGTGGGTTCTTGGGTGCGTGAGGCTGCTATGAATGGCCTTGAGAGATGTGCATATATCCTATGTAAGAGTGATTCTGTTGGTCTCACTGGAATATCAGGTCGAGTCGATTCTGCACTGGAGTTGCAGAACTGTGATGACAATAACCAGCTGCAGTTATTGTATGATGCCAATCTTGCTACCAATATAGTTGCTGGCATTAGTAAGCAAGCTGTAGAGAAAATGGACAAACTGAGAGAAGCAGCTGCAAAGGTTCTACAGAGGCTTTTGTACAACGAGATGGCCTATGTCCCACATATACCGCACAGGAAAAAATTGGAGGAAATTGTTCCTAATAAAGCTGATTTAAAGTGGGGGGTGCCAACTTTTTCATATCCTCGTTTTGTACAACTGCTTCAATTTGGCTGTTTTAGCAGATCAGTGCTATCTGGGCTAGTCATCTCTATTGGTGGGTTGCAAGATTCTTTGAGAAGGGCATCACTCACAGCATTGATAGAGTATCTTCAAGTTGTAGAAAGTGAAGACCAGAATGCAAGGTCCAGAGAGTATATGCTATCTACTGACATGCTTTGGGTTCTCCAACAGTATCGGAGATGTGACAGAGTCATTGTACCTGCATTGAAGACCATCGAGATTCTTTTCAGCAAACAGATATTTCTGAGTATGGAGGCTCATACACTTGTTTTTTGTTCTGGTGTTTTGGACTCTCTGGAAGTTGAATTAAAAGGGTCAAAGGACTTCTCCAAGCTATATGCAGGCATTGCAATACTTGGATATATTGCTTCAGTTTCGGAATCCATCAACACTCGGGCCTTCTCTCATCTTCTCAGTTTTCTTGGCCATCGCTACCCTAAGATACGGAAAGCTTCTGCTGAACAAGTTTACCTTGTCCTCTTGCAAAATGGGGGTCTTGTGGTTGAGAAAAAGATGGAGAAAGCGCTTGAAATTATTTCAGAAACCTGCTGGGAAGGTGACATGGAAGCTGCAAAAGTCCAAAGGTTGGAGTTGTATGATATAGCTGGTCTTGACACAGATATACTTCGAAAGGCTAGTAGTCGAGTATCAAATAAGGATGGTAACAGAAAGCCAACAACTACAGATGAAAATGCATCGTATTCCTCGTTAGTTGAGTCATCTGGGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005911 GO:0006457 GO:0006996 GO:0007010 GO:0007017 GO:0007021 GO:0007275 GO:0008047 GO:0008092 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0015630 GO:0015631 GO:0016043 GO:0022414 GO:0022607 GO:0030054 GO:0030234 GO:0030695 GO:0032501 GO:0032502 GO:0034622 GO:0043085 GO:0043087 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048487 GO:0048608 GO:0048731 GO:0048856 GO:0050790 GO:0051336 GO:0051345 GO:0055044 GO:0060589 GO:0061458 GO:0065003 GO:0065007 GO:0065009 GO:0071840 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TFCD_C

Protein Analysis

1273

Amino Acids

140.71

Weight (kDa)

5.79

Isoelectric Point (pI)

40.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ARM_TBCD	PF23579	71 - 261	5.4e-52	Tubulin-specific chaperone D-like, ARM repeat
ARM_TBCD_2nd	PF25767	319 - 566	7.3e-106	Tubulin-specific chaperone D-like, ARM repeats
TFCD_C	PF12612	953 - 1140	5.7e-57	Tubulin folding cofactor D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007907)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60740 AT3G60740 AT3G60740
fragaria_vesca	FvH4_7g07690
malus_domestica	MD02G1313300.v1.1 MD07G1008800.v1.1
prunus_persica	Prupe.2G141700_v2.0.a1 Prupe.2G141700_v2.0.a1 Prupe.2G141700_v2.0.a1 Prupe.2G141700_v2.0.a1
pyrus_communis	pycom07g00760
rosa_chinensis	RchiOBHm_Chr1g0342581
rosa_laevigata	RLG00000029165 RLG00000029175
rosa_multiflora	Rmu_sc0000173.1_g000001 Rmu_ssc0000177.1_g000008
rosa_roxburghii	Rroxscaffold_4G00313120
rosa_rugosa	Rorug01G0147200.1 Rorug01G0148800.1
rosa_samantha	Rh1AG165400 Rh1BG131400 Rh1CG154100 Rh1DG166500
rosa_wichuraiana	Rw1G013760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1910
Acc16I	TGCGCA	1 cut(s)	962
Acc36I	ACCTGC	2 cut(s)	3295, 3641
AccB1I	GGYRCC	2 cut(s)	83, 3031
AccB7I	CCANNNNNTGG	1 cut(s)	3673
AccBSI	CCGCTC	2 cut(s)	188, 274
AclWI	GGATC	5 cut(s)	173, 193, 198, 1501, 1889
AcoI	YGGCCR	3 cut(s)	1383, 1537, 3511
AcsI	RAATTY	6 cut(s)	327, 472, 676, 1827, 2215, 2238
AcuI	CTGAAG	6 cut(s)	303, 2277, 2285, 2462, 2514, 3441
AcyI	GRCGYC	1 cut(s)	84
AdeI	CACNNNGTG	1 cut(s)	1487
AfeI	AGCGCT	1 cut(s)	3612
AfiI	CCNNNNNNNGG	5 cut(s)	1303, 1346, 2359, 2424, 3673
AflIII	ACRYGT	3 cut(s)	728, 2050, 2589
AhlI	ACTAGT	1 cut(s)	238
AjiI	CACGTC	1 cut(s)	731
AjnI	CCWGG	2 cut(s)	252, 1339
AjuI	GAANNNNNNNTTGG	2 cut(s)	736, 768
AleI	CACNNNNGTG	2 cut(s)	1238, 2016
Alw21I	GWGCWC	1 cut(s)	102
Alw26I	GTCTC	6 cut(s)	163, 450, 1218, 1771, 2215, 2762
AlwI	GGATC	5 cut(s)	173, 193, 198, 1501, 1889
AlwNI	CAGNNNCTG	1 cut(s)	1646
Ama87I	CYCGRG	2 cut(s)	2547, 3480
Aor51HI	AGCGCT	1 cut(s)	3612
ApoI	RAATTY	6 cut(s)	327, 472, 676, 1827, 2215, 2238
ArsI	GACNNNNNNTTYG	4 cut(s)	464, 496, 3565, 3597
Asp700I	GAANNNNTTC	1 cut(s)	3000
AspLEI	GCGC	6 cut(s)	86, 693, 963, 1588, 1968, 3613
AspS9I	GGNCC	7 cut(s)	683, 747, 1309, 1478, 1576, 3210, 3483
AsuHPI	GGTGA	6 cut(s)	367, 1329, 1361, 2327, 2597, 3656
AsuII	TTCGAA	2 cut(s)	1471, 3715
AvaI	CYCGRG	2 cut(s)	2547, 3480
AvaII	GGWCC	4 cut(s)	1309, 1478, 1576, 3210
BalI	TGGCCA	1 cut(s)	3513
BanI	GGYRCC	2 cut(s)	83, 3031
BanII	GRGCYC	2 cut(s)	657, 953
BarI	GAAGNNNNNNTAC	2 cut(s)	3051, 3083
BauI	CACGAG	2 cut(s)	551, 2499
BbsI	GAAGAC	4 cut(s)	69, 1678, 3201, 3302
Bbv12I	GWGCWC	1 cut(s)	102
BbvCI	CCTCAGC	1 cut(s)	1866
BceAI	ACGGC	1 cut(s)	1524
BciT130I	CCWGG	2 cut(s)	254, 1341
BciVI	GTATCC	1 cut(s)	380
BclI	TGATCA	1 cut(s)	924
BcoDI	GTCTC	6 cut(s)	163, 450, 1218, 1771, 2215, 2762
BcuI	ACTAGT	1 cut(s)	238
BfaI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 3107, 3723
BfmI	CTRYAG	8 cut(s)	834, 1512, 2034, 2250, 2825, 2886, 2930, 3768
BfoI	RGCGCY	2 cut(s)	87, 3614
BfuAI	ACCTGC	2 cut(s)	3295, 3641
BfuI	GTATCC	1 cut(s)	380
BglI	GCCNNNNNGGC	4 cut(s)	14, 92, 1391, 1649
BlpI	GCTNAGC	1 cut(s)	1375
BmcAI	AGTACT	1 cut(s)	110
Bme1390I	CCNGG	2 cut(s)	254, 1341
Bme18I	GGWCC	4 cut(s)	1309, 1478, 1576, 3210
BmeT110I	CYCGRG	2 cut(s)	2547, 3480
BmgBI	CACGTC	1 cut(s)	731
BmgT120I	GGNCC	7 cut(s)	683, 747, 1309, 1478, 1576, 3210, 3483
BmiI	GGNNCC	4 cut(s)	85, 561, 950, 3033
BmrFI	CCNGG	2 cut(s)	254, 1341
BmrI	ACTGGG	2 cut(s)	1224, 1846
BmuI	ACTGGG	2 cut(s)	1224, 1846
BoxI	GACNNNNGTC	1 cut(s)	2524
BpiI	GAAGAC	4 cut(s)	69, 1678, 3201, 3302
BpmI	CTGGAG	1 cut(s)	2813
Bpu10I	CCTNAGC	1 cut(s)	1866
Bpu1102I	GCTNAGC	1 cut(s)	1375
Bpu14I	TTCGAA	2 cut(s)	1471, 3715
BpuEI	CTTGAG	3 cut(s)	641, 2365, 2735
BsaAI	YACGTR	1 cut(s)	1397
BsaBI	GATNNNNATC	1 cut(s)	2844
BsaHI	GRCGYC	1 cut(s)	84
BsaI	GGTCTC	1 cut(s)	2762
BsaJI	CCNNGG	5 cut(s)	9, 87, 743, 1969, 2668
BsaWI	WCCGGW	1 cut(s)	819
BsaXI	ACNNNNNCTCC	4 cut(s)	1050, 1080, 1298, 1328
Bsc4I	CCNNNNNNNGG	5 cut(s)	1303, 1346, 2359, 2424, 3673
Bse3DI	GCAATG	4 cut(s)	57, 591, 936, 3432
Bse8I	GATNNNNATC	1 cut(s)	2844
BseBI	CCWGG	2 cut(s)	254, 1341
BseDI	CCNNGG	5 cut(s)	9, 87, 743, 1969, 2668
BseGI	GGATG	7 cut(s)	1177, 1513, 1813, 2251, 2437, 2620, 3751
BseJI	GATNNNNATC	1 cut(s)	2844
BseLI	CCNNNNNNNGG	5 cut(s)	1303, 1346, 2359, 2424, 3673
BseMI	GCAATG	4 cut(s)	57, 591, 936, 3432
BseMII	CTCAG	4 cut(s)	1857, 2897, 3323, 3514
BseRI	GAGGAG	1 cut(s)	1319
BseYI	CCCAGC	2 cut(s)	1988, 3636
BsgI	GTGCAG	3 cut(s)	1994, 2142, 2772
Bsh1285I	CGRYCG	1 cut(s)	1618
BshNI	GGYRCC	2 cut(s)	83, 3031
BsiEI	CGRYCG	1 cut(s)	1618
BsiHKAI	GWGCWC	1 cut(s)	102
BsiHKCI	CYCGRG	2 cut(s)	2547, 3480
BsiSI	CCGG	1 cut(s)	820
BslFI	GGGAC	1 cut(s)	2948
BslI	CCNNNNNNNGG	5 cut(s)	1303, 1346, 2359, 2424, 3673
BsmAI	GTCTC	6 cut(s)	163, 450, 1218, 1771, 2215, 2762
BsmBI	CGTCTC	1 cut(s)	163
BsmFI	GGGAC	1 cut(s)	2948
BsmI	GAATGC	1 cut(s)	3208
Bso31I	GGTCTC	1 cut(s)	2762
BsoBI	CYCGRG	2 cut(s)	2547, 3480
Bsp119I	TTCGAA	2 cut(s)	1471, 3715
Bsp1286I	GDGCHC	3 cut(s)	102, 657, 953
Bsp1407I	TGTACA	2 cut(s)	2943, 3058
Bsp143I	GATC	7 cut(s)	178, 198, 203, 924, 1493, 1894, 3089
Bsp1720I	GCTNAGC	1 cut(s)	1375
Bsp19I	CCATGG	2 cut(s)	9, 1969
BspCNI	CTCAG	4 cut(s)	1858, 2898, 3324, 3513
BspLI	GGNNCC	4 cut(s)	85, 561, 950, 3033
BspMAI	CTGCAG	4 cut(s)	1516, 2038, 2254, 2829
BspMI	ACCTGC	2 cut(s)	3295, 3641
BspPI	GGATC	5 cut(s)	173, 193, 198, 1501, 1889
BspQI	GCTCTTC	1 cut(s)	1630
BspT104I	TTCGAA	2 cut(s)	1471, 3715
BspT107I	GGYRCC	2 cut(s)	83, 3031
BspTNI	GGTCTC	1 cut(s)	2762
BsrBI	CCGCTC	2 cut(s)	188, 274
BsrDI	GCAATG	4 cut(s)	57, 591, 936, 3432
BsrGI	TGTACA	2 cut(s)	2943, 3058
BssECI	CCNNGG	5 cut(s)	9, 87, 743, 1969, 2668
BssMI	GATC	7 cut(s)	178, 198, 203, 924, 1493, 1894, 3089
BssNI	GRCGYC	1 cut(s)	84
BssSI	CACGAG	2 cut(s)	551, 2499
BssT1I	CCWWGG	4 cut(s)	9, 87, 743, 1969
Bst2BI	CACGAG	2 cut(s)	551, 2499
Bst2UI	CCWGG	2 cut(s)	254, 1341
Bst4CI	ACNGT	9 cut(s)	301, 379, 580, 605, 1237, 1603, 1619, 2807, 3258
Bst6I	CTCTTC	4 cut(s)	1185, 1468, 1630, 2365
BstACI	GRCGYC	1 cut(s)	84
BstAPI	GCANNNNNTGC	4 cut(s)	848, 1646, 2275, 3434
BstAUI	TGTACA	2 cut(s)	2943, 3058
BstBAI	YACGTR	1 cut(s)	1397
BstBI	TTCGAA	2 cut(s)	1471, 3715
BstDEI	CTNAG	6 cut(s)	1375, 1866, 2906, 3332, 3500, 3525
BstDSI	CCRYGG	2 cut(s)	9, 1969
BstENI	CCTNNNNNAGG	1 cut(s)	1301
BstF5I	GGATG	7 cut(s)	1177, 1513, 1813, 2251, 2437, 2620, 3751
BstH2I	RGCGCY	2 cut(s)	87, 3614
BstHHI	GCGC	6 cut(s)	86, 693, 963, 1588, 1968, 3613
BstKTI	GATC	7 cut(s)	181, 201, 206, 927, 1496, 1897, 3092
BstMAI	GTCTC	6 cut(s)	163, 450, 1218, 1771, 2215, 2762
BstMBI	GATC	7 cut(s)	178, 198, 203, 924, 1493, 1894, 3089
BstMCI	CGRYCG	1 cut(s)	1618
BstNI	CCWGG	2 cut(s)	254, 1341
BstNSI	RCATGY	4 cut(s)	742, 1966, 2593, 3241
BstPAI	GACNNNNGTC	1 cut(s)	2524
BstSCI	CCNGG	2 cut(s)	252, 1339
BstSFI	CTRYAG	8 cut(s)	834, 1512, 2034, 2250, 2825, 2886, 2930, 3768
BstV2I	GAAGAC	4 cut(s)	69, 1678, 3201, 3302
BstX2I	RGATCY	2 cut(s)	198, 1493
BstYI	RGATCY	2 cut(s)	198, 1493
BsuI	GTATCC	1 cut(s)	380
BtgI	CCRYGG	2 cut(s)	9, 1969
BtgZI	GCGATG	2 cut(s)	2688, 3500
BtrI	CACGTC	1 cut(s)	731
BtsCI	GGATG	7 cut(s)	1177, 1513, 1813, 2251, 2437, 2620, 3751
BtsI	GCAGTG	5 cut(s)	102, 485, 1710, 2006, 2168
BveI	ACCTGC	2 cut(s)	3295, 3641
CaiI	CAGNNNCTG	1 cut(s)	1646
CfoI	GCGC	6 cut(s)	86, 693, 963, 1588, 1968, 3613
Cfr13I	GGNCC	7 cut(s)	683, 747, 1309, 1478, 1576, 3210, 3483
CspCI	CAANNNNNGTGG	4 cut(s)	621, 656, 847, 882
DdeI	CTNAG	6 cut(s)	1375, 1866, 2906, 3332, 3500, 3525
DinI	GGCGCC	1 cut(s)	85
DpnI	GATC	7 cut(s)	180, 200, 205, 926, 1495, 1896, 3091
DpnII	GATC	7 cut(s)	178, 198, 203, 924, 1493, 1894, 3089
DraI	TTTAAA	1 cut(s)	3021
DraIII	CACNNNGTG	1 cut(s)	1487
EaeI	YGGCCR	3 cut(s)	1383, 1537, 3511
Eam1104I	CTCTTC	4 cut(s)	1185, 1468, 1630, 2365
EarI	CTCTTC	4 cut(s)	1185, 1468, 1630, 2365
EciI	GGCGGA	3 cut(s)	32, 886, 2121
Eco130I	CCWWGG	4 cut(s)	9, 87, 743, 1969
Eco24I	GRGCYC	2 cut(s)	657, 953
Eco31I	GGTCTC	1 cut(s)	2762
Eco32I	GATATC	1 cut(s)	571
Eco47I	GGWCC	4 cut(s)	1309, 1478, 1576, 3210
Eco47III	AGCGCT	1 cut(s)	3612
Eco57I	CTGAAG	6 cut(s)	303, 2277, 2285, 2462, 2514, 3441
Eco88I	CYCGRG	2 cut(s)	2547, 3480
EcoNI	CCTNNNNNAGG	1 cut(s)	1301
EcoO109I	RGGNCCY	3 cut(s)	683, 747, 1309
EcoRII	CCWGG	2 cut(s)	252, 1339
EcoRV	GATATC	1 cut(s)	571
EcoT14I	CCWWGG	4 cut(s)	9, 87, 743, 1969
EcoT22I	ATGCAT	1 cut(s)	3784
EcoT38I	GRGCYC	2 cut(s)	657, 953
EgeI	GGCGCC	1 cut(s)	85
EheI	GGCGCC	1 cut(s)	85
ErhI	CCWWGG	4 cut(s)	9, 87, 743, 1969
Esp3I	CGTCTC	1 cut(s)	163
FalI	AAGNNNNNCTT	8 cut(s)	128, 160, 654, 686, 2073, 2105, 3543, 3575
FaqI	GGGAC	1 cut(s)	2948
FauI	CCCGC	3 cut(s)	162, 279, 2141
FauNDI	CATATG	2 cut(s)	350, 1519
FbaI	TGATCA	1 cut(s)	924
FokI	GGATG	7 cut(s)	1184, 1520, 1820, 2258, 2444, 2627, 3758
FriOI	GRGCYC	2 cut(s)	657, 953
FspBI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 3107, 3723
FspI	TGCGCA	1 cut(s)	962
GlaI	GCGC	6 cut(s)	85, 692, 962, 1587, 1967, 3612
GsaI	CCCAGC	2 cut(s)	1992, 3640
GsuI	CTGGAG	1 cut(s)	2813
HaeII	RGCGCY	2 cut(s)	87, 3614
HapII	CCGG	1 cut(s)	820
HhaI	GCGC	6 cut(s)	86, 693, 963, 1588, 1968, 3613
Hin1I	GRCGYC	1 cut(s)	84
Hin6I	GCGC	6 cut(s)	84, 691, 961, 1586, 1966, 3611
HinP1I	GCGC	6 cut(s)	84, 691, 961, 1586, 1966, 3611
HincII	GTYRAC	2 cut(s)	236, 910
HindII	GTYRAC	2 cut(s)	236, 910
HindIII	AAGCTT	3 cut(s)	719, 2444, 3537
HinfI	GANTC	9 cut(s)	2747, 2778, 2783, 3134, 3276, 3307, 3377, 3467, 3803
HpaII	CCGG	1 cut(s)	820
HphI	GGTGA	6 cut(s)	367, 1329, 1361, 2327, 2597, 3656
Hpy166II	GTNNAC	7 cut(s)	236, 910, 1019, 1481, 1708, 2408, 3556
Hpy8I	GTNNAC	7 cut(s)	236, 910, 1019, 1481, 1708, 2408, 3556
Hpy99I	CGWCG	1 cut(s)	77
HpyAV	CCTTC	9 cut(s)	125, 1032, 1433, 1444, 1604, 2422, 3138, 3496, 3635
HpyCH4III	ACNGT	9 cut(s)	301, 379, 580, 605, 1237, 1603, 1619, 2807, 3258
HpyCH4IV	ACGT	4 cut(s)	730, 1065, 1396, 2050
HpyF3I	CTNAG	6 cut(s)	1375, 1866, 2906, 3332, 3500, 3525
HpySE526I	ACGT	4 cut(s)	730, 1065, 1396, 2050
Hsp92I	GRCGYC	1 cut(s)	84
HspAI	GCGC	6 cut(s)	84, 691, 961, 1586, 1966, 3611
KasI	GGCGCC	1 cut(s)	83
Ksp22I	TGATCA	1 cut(s)	924
Kzo9I	GATC	7 cut(s)	178, 198, 203, 924, 1493, 1894, 3089
LguI	GCTCTTC	1 cut(s)	1630
LmnI	GCTCC	7 cut(s)	105, 193, 634, 830, 948, 1327, 1973
MaeI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 3107, 3723
MaeII	ACGT	4 cut(s)	730, 1065, 1396, 2050
MalI	GATC	7 cut(s)	180, 200, 205, 926, 1495, 1896, 3091
MbiI	CCGCTC	2 cut(s)	188, 274
MboI	GATC	7 cut(s)	178, 198, 203, 924, 1493, 1894, 3089
MflI	RGATCY	2 cut(s)	198, 1493
MhlI	GDGCHC	3 cut(s)	102, 657, 953
MlsI	TGGCCA	1 cut(s)	3513
MluNI	TGGCCA	1 cut(s)	3513
Mly113I	GGCGCC	1 cut(s)	84
MlyI	GAGTC	4 cut(s)	2787, 3285, 3371, 3812
MmeI	TCCRAC	7 cut(s)	1152, 1305, 1471, 1648, 2284, 3277, 3654
Mox20I	TGGCCA	1 cut(s)	3513
Mph1103I	ATGCAT	1 cut(s)	3784
MroXI	GAANNNNTTC	1 cut(s)	3000
MscI	TGGCCA	1 cut(s)	3513
MslI	CAYNNNNRTG	4 cut(s)	1024, 1238, 1559, 2016
Msp20I	TGGCCA	1 cut(s)	3513
MspA1I	CMGCKG	6 cut(s)	1646, 1988, 2126, 2255, 2824, 2918
MspI	CCGG	1 cut(s)	820
MspR9I	CCNGG	2 cut(s)	254, 1341
Mva1269I	GAATGC	1 cut(s)	3208
MvaI	CCWGG	2 cut(s)	254, 1341
NarI	GGCGCC	1 cut(s)	84
NcoI	CCATGG	2 cut(s)	9, 1969
NdeI	CATATG	2 cut(s)	350, 1519
NdeII	GATC	7 cut(s)	178, 198, 203, 924, 1493, 1894, 3089
NlaIV	GGNNCC	4 cut(s)	85, 561, 950, 3033
NmuCI	GTSAC	8 cut(s)	373, 771, 1832, 2166, 2348, 2585, 3269, 3644
NsbI	TGCGCA	1 cut(s)	962
NsiI	ATGCAT	1 cut(s)	3784
NspI	RCATGY	4 cut(s)	742, 1966, 2593, 3241
NspV	TTCGAA	2 cut(s)	1471, 3715
OliI	CACNNNNGTG	2 cut(s)	1238, 2016
PaeI	GCATGC	1 cut(s)	1966
PciI	ACATGT	1 cut(s)	2589
PciSI	GCTCTTC	1 cut(s)	1630
PcsI	WCGNNNNNNNCGW	3 cut(s)	72, 2092, 3791
PctI	GAATGC	1 cut(s)	3208
PdmI	GAANNNNTTC	1 cut(s)	3000
PfeI	GAWTC	5 cut(s)	2747, 2783, 3134, 3307, 3467
PflFI	GACNNNGTC	2 cut(s)	155, 3275
PflMI	CCANNNNNTGG	1 cut(s)	3673
PleI	GAGTC	4 cut(s)	2786, 3284, 3371, 3811
PluTI	GGCGCC	1 cut(s)	87
PpsI	GAGTC	4 cut(s)	2786, 3284, 3371, 3811
Ppu21I	YACGTR	1 cut(s)	1397
PpuMI	RGGWCCY	1 cut(s)	1309
PscI	ACATGT	1 cut(s)	2589
PshAI	GACNNNNGTC	1 cut(s)	2524
PsiI	TTATAA	1 cut(s)	1910
Psp5II	RGGWCCY	1 cut(s)	1309
Psp6I	CCWGG	2 cut(s)	252, 1339
PspFI	CCCAGC	2 cut(s)	1988, 3636
PspGI	CCWGG	2 cut(s)	252, 1339
PspN4I	GGNNCC	4 cut(s)	85, 561, 950, 3033
PspPI	GGNCC	7 cut(s)	683, 747, 1309, 1478, 1576, 3210, 3483
PspPPI	RGGWCCY	1 cut(s)	1309
PstI	CTGCAG	4 cut(s)	1516, 2038, 2254, 2829
PstNI	CAGNNNCTG	1 cut(s)	1646
PsuI	RGATCY	2 cut(s)	198, 1493
PsyI	GACNNNGTC	2 cut(s)	155, 3275
PvuII	CAGCTG	5 cut(s)	1988, 2126, 2255, 2824, 2918
RseI	CAYNNNNRTG	4 cut(s)	1024, 1238, 1559, 2016
SapI	GCTCTTC	1 cut(s)	1630
Sau3AI	GATC	7 cut(s)	178, 198, 203, 924, 1493, 1894, 3089
Sau96I	GGNCC	7 cut(s)	683, 747, 1309, 1478, 1576, 3210, 3483
ScaI	AGTACT	1 cut(s)	110
SchI	GAGTC	4 cut(s)	2787, 3285, 3371, 3812
ScrFI	CCNGG	2 cut(s)	254, 1341
SduI	GDGCHC	3 cut(s)	102, 657, 953
SfcI	CTRYAG	8 cut(s)	834, 1512, 2034, 2250, 2825, 2886, 2930, 3768
SfoI	GGCGCC	1 cut(s)	85
SfuI	TTCGAA	2 cut(s)	1471, 3715
SinI	GGWCC	4 cut(s)	1309, 1478, 1576, 3210
SmiMI	CAYNNNNRTG	4 cut(s)	1024, 1238, 1559, 2016
SmlI	CTYRAG	3 cut(s)	620, 2344, 2714
SmoI	CTYRAG	3 cut(s)	620, 2344, 2714
SpeI	ACTAGT	1 cut(s)	238
SphI	GCATGC	1 cut(s)	1966
SspDI	GGCGCC	1 cut(s)	83
SspMI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 3107, 3723
StyD4I	CCNGG	2 cut(s)	252, 1339
StyI	CCWWGG	4 cut(s)	9, 87, 743, 1969
TaaI	ACNGT	9 cut(s)	301, 379, 580, 605, 1237, 1603, 1619, 2807, 3258
TaiI	ACGT	4 cut(s)	733, 1068, 1399, 2053
TaqI	TCGA	9 cut(s)	716, 1096, 1471, 2337, 2776, 2781, 3303, 3715, 3730
TatI	WGTACW	4 cut(s)	108, 500, 2943, 3058
TauI	GCSGC	6 cut(s)	493, 944, 1272, 1646, 1652, 1865
TfiI	GAWTC	5 cut(s)	2747, 2783, 3134, 3307, 3467
TseFI	GTSAC	8 cut(s)	373, 771, 1832, 2166, 2348, 2585, 3269, 3644
Tsp45I	GTSAC	8 cut(s)	373, 771, 1832, 2166, 2348, 2585, 3269, 3644
TspGWI	ACGGA	4 cut(s)	552, 1244, 1571, 3547
Tth111I	GACNNNGTC	2 cut(s)	155, 3275
Van91I	CCANNNNNTGG	1 cut(s)	3673
VpaK11BI	GGWCC	4 cut(s)	1309, 1478, 1576, 3210
XagI	CCTNNNNNAGG	1 cut(s)	1301
XapI	RAATTY	6 cut(s)	327, 472, 676, 1827, 2215, 2238
XceI	RCATGY	4 cut(s)	742, 1966, 2593, 3241
XmnI	GAANNNNTTC	1 cut(s)	3000
XspI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 3107, 3723
ZrmI	AGTACT	1 cut(s)	110
Zsp2I	ATGCAT	1 cut(s)	3784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.