Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007907

Physical Location & Seq

Genomic Coordinates Reverse (-)

821387 .. 831928

Gene Structure 10542 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1008800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3819 bp

ATGGCAACCATGGCGGCGGAAGAAAACCCACAGCAGAAACAGGAACTTGCAATGGGTGAGGAAGAAGACGACGACGAGCATGGTGCCAAGGAGGCAGTACTCCAAAAGTACTTCCTTCAAGAATGGAAGCTCGTCAAGTCCATCTTAGACGACATTGTTTCCAATGGGCGGGTCTCCGATCCCTCCGCTCCCCACAAGATCCGATCCATTATGGACAAGTATCAAGAACAGGGCCAGCTAGTTGAACCGTACCTGGAGAGCATTGTTACCCCACTTATGTTTATTGTCCGCTCTAAAACAAGCGAATTAGGTGTAGCTTCGGATGAAATTCTTGAAGTAATTAAACCTATATGCATCATTCTTTATTCTCTGGTCACAGTTTGTGGATACAAGGCGGTTGTCAGGTTCTTTCCGCATCAAGTCTCGGACTTGGAACTTGCTGTGTCTCTCTTGGAGAAGTGCCATCACACAAAGTCAGTGTCATCACTGCGTCAGGAAAGTACAGGAGAGATGGAAGCTAAATGTGTGATGCTTTTGTGGCTGTCTATACTCGTGTTGGTTCCGTTTGATATCTCCACTGTTGATACTAGCATTGCAAACAACAGTAATCTTGGAAAACTTGAGCCAGCTCCTCTGGTATTGAGGATAGTAGGGTTCTCCAAAGATTACCTTTCAAATTCAGGCCCTATGCGTCCTATTGCTGCTTTGCTGCTCTCGAAGCTTCTAACACGCCCAGACATGCCAAAGGCTTTTTCCAGCTTTGTTGAATGGACACACGAAGTACTATCTTCTCTTACAGATGATGCCATCAATCATATCCGGTTACTTGGAGCTACAGAAGCACTGGCTGCTATTTTCAAGGTTGGTGGGCGGAAACTCTTGCTTGATGTGGTTCCTATTGTTTGGGTTGACACCTTATTGTTGATCAAGTCTAGCAATGCAGCTAGGAGCCCATTGCTGCGCAAGTATCTGATGAAATTAACCCAGAGAATTGGGCTTACTTGCCTTCCTCATCGTACACCTTCATGGCGCTATGTGGGGAAAACAAGCTCTTTGGGAGAGAACATAACTTTGTCTGCATCTGAAAAAACTGGTCGATGCAATTATGCTTTAAATTCTGAGGATTCCAACTCAGAACCAAGTTCAAGCTGCCAGCAAGATGAAGAAATGGATGTTCCAGAAGTTGTAGAAGAGATTATTGAGATGCTACTTACTGGTTTGAGAGATACGGACACTGTTGTGCGTTGGTCTGCTGCAAAAGGTATTGGCCGCATTACTTCATGTCTTACATCTGCCCTTTCAGAGGAGGTCCTGTCATCTGTGTTGGAGCTGTTTTCTCCAGGCGAGGGTGATGGGTCATGGCATGGTGGTTGCTTAGCACTGGCTGAATTGGCGCGTAGAGGGTTGCTCTTACCTATCAGCCTTCCGAAAGTTGTACCTGTTGTTGTGAAGGCACTGCATTATGATATTCGAAGAGGTCCACATAGTATTGGATCTCATGTACGGGATGCTGCAGCTTATGTTTGTTGGGCTTTTGGCCGTGCATATTATCACACGGATATGAGAAATATACTGGACCAGCTTGCACCACACCTTCTGACAGTGGCCTGCTACGACCGTGAGGTTAATTGTAGAAGAGCAGCTGCTGCAGCTTTTCAAGAGAATGTTGGAAGGCAGGGTAGTTACCCTCATGGAATTGACATAGTGAACACTGCAGACTATTTCTCACTTTCTTCACGAGTAAACTCTTATGTTCATGTTGCTGTCTCTATTGCTCAATATGAGGGTTATCTTTATCCATTTGTGGATGAGCTGCTGTATAACAAAATTTGTCACTGGGATAAAGGTTTGAGAGAACTTGCGGCTGAGGCTCTTTCTTCCCTGGTTAAATATGATCATGACTATCTTGCAAACTATGCTGTGGAGAAAATAATTCCTTGCACTCTCTCATCTGATTTGTGCATGCGCCATGGAGCAACACTGGCAGCTGGGGAACTTGTTTTGGCACTACACAAGTGTGGTTATGCTCTTTCTGCAGATAAGCAAAAACGTGTTGCTGGTGTTGTGCCTGCTATTGAGAAAGCACGCCTTTATCGTGGAAAAGGCGGGGAAATAATGCGTGCAGCTGTTTCCCGCTTCATTGAATGTATATCTATATCATCTGTGTCACTGCCTGAAAAGATAAAACGTAGTCTGCTTGATACTCTTAATGAGAATTTGAGACACCCTAATTCGCAAATTCAGGATGCTGCAACTAAAGCTCTGAAGCATTTTGTGCAAGCATATCTGGTTGCTGGGTCTGTTGGAGGTACTGGTGATATAACATCAAAGTACCTGGACCTCTTGAGTGACCCAAATGTGGCTATAAGAAGAGGGTCAGCACTAGCGCTAGGTGTTTTGCCATGTGAACTTTTCGCCCATAGGTGGAAGGATGTGCTTCTGAAGCTTTGTAACTCTTGTTTAATTGAGGATAATCCCGATGATAGAGATGCTGAAGCACGAGTCAATGCTGTCAAAGGACTTGTGTCAGTGTGTGAAGCATTAACTCGGGAAAAAGAACAATCTGGAATTGATGCCGTGGAGGGTGACATGTCTTTGTTTATTCTCATCAAGGACGAAATAATGATGACTTTACTTAAGGCTCTTGATGACTATTCTGTTGATAACAGAGGTGATGTGGGTTCTTGGGTGCGTGAGGCTGCTATGAATGGCCTTGAGAGATGTACATATATCCTCTGTAAGAGAGATTCTGTTGGTCTTACTGGAAGATCAGGTCGAGTCGATTCTGCACTGGAGTTGCAGAATAGTGATGACATTAACCAGCTGCAATCATTGTATGATGCCAATCTTGCTACAAGTATAGTTGCTGGAATTAGTAAGCAAGCTGTAGAGAAAATGGACAAACTGAGAGAAGCAGCTGCAAAGGTTCTACAGAGGATTTTGTACAACGAGATAGCCTATGTCCCGCATATACCGCACAGAAAAAAGTTGGAAAAAATTGTTCCTAACGGAGCTGATTTAAAGTGGGGGGTGCCAACTTTTTCATATCCTCGTTTTGTACAACTGCTTCAATTTGGCTGTTATAGCAGATCAGTGCTATCTGGGTTAGTCATCTCTATTGGTGGGTTGCAAGATTTTTTGAGGAAGGCATCACTCACAGCATTGTTAGAGTATCTTCAAGTTGTAGAAAGTGAAGACCAGAACGAAAGGTCCAGAGAGTATATGTTATCTACTGACATGCTTTGGGTTCTCCAACAGTACAGGAGATGTGACAGAGTCATTGTACCTGCATTGAAGACGATTGAGATTCTTTTCAGCAAACAGATATTGTTGAGTATGGAGGCTCATACACTTTTTTTTTGTACTGGTGTTTTGGACTCTCTGGAAGTTGAATTAAAAGGGTCAAGGGACTTCTCCAAGCTATATGCAGGCATTGCAATACTTGGATATATTGCTTCAGTTTCGGAGTCCATCAACACTCGGGCCTTCTCACATCTTCTCAGTTTTCTTGGCCATCGCTACCCTAAGATACGGAAAGCTTCTGCTGAACAAGTTTACCTTGTCCTCCTGCAAAATGAGGGTCTTGTGGCCGAGACCAAGGTGGAAAAAGCGCTTGAAATTATTTCAGAAACCTGCTGGGAAGGTGACATGGAAGCAGCTAAAATCCGAAGGTTGGAGTTGTATGATATAGCCGGTCTCGACACAGATATACTTCGGAAGGCTAGTAGCCGAGAATCGAATAAGGATGGTAACAGAAAGCCAACAACTACAGATGAGAATGCATCATATTCCTCGTTAGTTGAGTCATCTGGGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005911 GO:0006457 GO:0006996 GO:0007010 GO:0007017 GO:0007021 GO:0007275 GO:0008047 GO:0008092 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0015630 GO:0015631 GO:0016043 GO:0022414 GO:0022607 GO:0030054 GO:0030234 GO:0030695 GO:0032501 GO:0032502 GO:0034622 GO:0043085 GO:0043087 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048487 GO:0048608 GO:0048731 GO:0048856 GO:0050790 GO:0051336 GO:0051345 GO:0055044 GO:0060589 GO:0061458 GO:0065003 GO:0065007 GO:0065009 GO:0071840 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TFCD_C

Protein Analysis

1273

Amino Acids

140.94

Weight (kDa)

5.87

Isoelectric Point (pI)

43.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ARM_TBCD	PF23579	71 - 261	2.1e-53	Tubulin-specific chaperone D-like, ARM repeat
ARM_TBCD_2nd	PF25767	319 - 566	1.7e-108	Tubulin-specific chaperone D-like, ARM repeats
TFCD_C	PF12612	953 - 1140	1.4e-54	Tubulin folding cofactor D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007907)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60740 AT3G60740 AT3G60740
fragaria_vesca	FvH4_7g07690
malus_domestica	MD02G1313300.v1.1 MD07G1008800.v1.1
prunus_persica	Prupe.2G141700_v2.0.a1 Prupe.2G141700_v2.0.a1 Prupe.2G141700_v2.0.a1 Prupe.2G141700_v2.0.a1
pyrus_communis	pycom07g00760
rosa_chinensis	RchiOBHm_Chr1g0342581
rosa_laevigata	RLG00000029165 RLG00000029175
rosa_multiflora	Rmu_sc0000173.1_g000001 Rmu_ssc0000177.1_g000008
rosa_roxburghii	Rroxscaffold_4G00313120
rosa_rugosa	Rorug01G0147200.1 Rorug01G0148800.1
rosa_samantha	Rh1AG165400 Rh1BG131400 Rh1CG154100 Rh1DG166500
rosa_wichuraiana	Rw1G013760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	962
Acc36I	ACCTGC	2 cut(s)	3295, 3641
AccB1I	GGYRCC	2 cut(s)	83, 3031
AccBSI	CCGCTC	2 cut(s)	188, 291
AccII	CGCG	1 cut(s)	1396
AclWI	GGATC	4 cut(s)	173, 193, 198, 1501
AcoI	YGGCCR	4 cut(s)	1267, 1537, 3511, 3588
AcsI	RAATTY	6 cut(s)	327, 676, 1114, 1827, 2215, 2238
AcuI	CTGAAG	4 cut(s)	2285, 2462, 2514, 3441
AfeI	AGCGCT	2 cut(s)	2388, 3612
AfiI	CCNNNNNNNGG	6 cut(s)	168, 1303, 1346, 2359, 2424, 3673
AflII	CTTAAG	1 cut(s)	2636
AflIII	ACRYGT	2 cut(s)	2050, 2589
AjnI	CCWGG	4 cut(s)	252, 1339, 1881, 2334
AjuI	GAANNNNNNNTTGG	2 cut(s)	736, 768
AleI	CACNNNNGTG	2 cut(s)	1238, 2016
AloI	GAACNNNNNNTCC	2 cut(s)	3194, 3226
Alw26I	GTCTC	7 cut(s)	178, 427, 450, 1771, 2215, 3587, 3701
AlwI	GGATC	4 cut(s)	173, 193, 198, 1501
AlwNI	CAGNNNCTG	1 cut(s)	1646
Ama87I	CYCGRG	2 cut(s)	2547, 3480
Aor51HI	AGCGCT	2 cut(s)	2388, 3612
AoxI	GGCC	9 cut(s)	232, 682, 1267, 1537, 1605, 2710, 3483, 3511, 3588
ApoI	RAATTY	6 cut(s)	327, 676, 1114, 1827, 2215, 2238
ArsI	GACNNNNNNTTYG	4 cut(s)	464, 496, 3565, 3597
AspLEI	GCGC	6 cut(s)	963, 1032, 1396, 1968, 2389, 3613
AspS9I	GGNCC	8 cut(s)	232, 683, 1309, 1478, 1576, 2338, 3210, 3483
AsuHPI	GGTGA	6 cut(s)	68, 1361, 2327, 2597, 2684, 3656
AsuII	TTCGAA	1 cut(s)	1471
AvaI	CYCGRG	2 cut(s)	2547, 3480
AvaII	GGWCC	5 cut(s)	1309, 1478, 1576, 2338, 3210
BalI	TGGCCA	1 cut(s)	3513
BanI	GGYRCC	2 cut(s)	83, 3031
BanII	GRGCYC	1 cut(s)	953
BarI	GAAGNNNNNNTAC	2 cut(s)	3051, 3083
BauI	CACGAG	3 cut(s)	551, 1737, 2499
BbsI	GAAGAC	3 cut(s)	72, 3201, 3302
BbvCI	CCTCAGC	1 cut(s)	1866
BccI	CCATC	8 cut(s)	149, 471, 505, 815, 1346, 3479, 3522, 3740
BceAI	ACGGC	2 cut(s)	1524, 2561
BcgI	CGANNNNNNTGC	2 cut(s)	65, 99
BciT130I	CCWGG	4 cut(s)	254, 1341, 1883, 2336
BciVI	GTATCC	1 cut(s)	380
BclI	TGATCA	2 cut(s)	924, 1894
BcoDI	GTCTC	7 cut(s)	178, 427, 450, 1771, 2215, 3587, 3701
BfaI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 2390, 3723
BfmI	CTRYAG	8 cut(s)	834, 1512, 1647, 1713, 2034, 2886, 2930, 3768
BfoI	RGCGCY	3 cut(s)	1033, 2390, 3614
BfrI	CTTAAG	1 cut(s)	2636
BfuAI	ACCTGC	2 cut(s)	3295, 3641
BfuI	GTATCC	1 cut(s)	380
BglI	GCCNNNNNGGC	1 cut(s)	92
BlpI	GCTNAGC	1 cut(s)	1375
BmcAI	AGTACT	3 cut(s)	99, 110, 783
Bme1390I	CCNGG	4 cut(s)	254, 1341, 1883, 2336
Bme18I	GGWCC	5 cut(s)	1309, 1478, 1576, 2338, 3210
BmeT110I	CYCGRG	2 cut(s)	2547, 3480
BmgT120I	GGNCC	8 cut(s)	232, 683, 1309, 1478, 1576, 2338, 3210, 3483
BmiI	GGNNCC	5 cut(s)	85, 561, 894, 950, 3033
BmrFI	CCNGG	4 cut(s)	254, 1341, 1883, 2336
BmrI	ACTGGG	1 cut(s)	1846
BmuI	ACTGGG	1 cut(s)	1846
BoxI	GACNNNNGTC	1 cut(s)	2524
BpiI	GAAGAC	3 cut(s)	72, 3201, 3302
BplI	GAGNNNNNCTC	2 cut(s)	1392, 1424
BpmI	CTGGAG	3 cut(s)	275, 1323, 2813
Bpu10I	CCTNAGC	1 cut(s)	1866
Bpu1102I	GCTNAGC	1 cut(s)	1375
Bpu14I	TTCGAA	1 cut(s)	1471
BpuEI	CTTGAG	3 cut(s)	641, 2365, 2735
BsaBI	GATNNNNATC	1 cut(s)	2844
BsaI	GGTCTC	3 cut(s)	178, 3587, 3701
BsaJI	CCNNGG	6 cut(s)	9, 87, 1881, 1969, 2577, 3597
BsaWI	WCCGGW	1 cut(s)	819
BsaXI	ACNNNNNCTCC	2 cut(s)	1298, 1328
Bsc4I	CCNNNNNNNGG	6 cut(s)	168, 1303, 1346, 2359, 2424, 3673
Bse118I	RCCGGY	1 cut(s)	3692
Bse3DI	GCAATG	5 cut(s)	57, 591, 943, 953, 3432
Bse8I	GATNNNNATC	1 cut(s)	2844
BseBI	CCWGG	4 cut(s)	254, 1341, 1883, 2336
BseDI	CCNNGG	6 cut(s)	9, 87, 1881, 1969, 2577, 3597
BseGI	GGATG	7 cut(s)	328, 1177, 1513, 1813, 2251, 2437, 3751
BseJI	GATNNNNATC	1 cut(s)	2844
BseLI	CCNNNNNNNGG	6 cut(s)	168, 1303, 1346, 2359, 2424, 3673
BseMI	GCAATG	5 cut(s)	57, 591, 943, 953, 3432
BseMII	CTCAG	5 cut(s)	1110, 1146, 1857, 2897, 3514
BseRI	GAGGAG	2 cut(s)	621, 1319
BseYI	CCCAGC	3 cut(s)	1988, 2294, 3636
BsgI	GTGCAG	2 cut(s)	2142, 2772
Bsh1236I	CGCG	1 cut(s)	1396
Bsh1285I	CGRYCG	1 cut(s)	1618
BshFI	GGCC	9 cut(s)	234, 684, 1269, 1539, 1607, 2712, 3485, 3513, 3590
BshNI	GGYRCC	2 cut(s)	83, 3031
BsiEI	CGRYCG	1 cut(s)	1618
BsiHKCI	CYCGRG	2 cut(s)	2547, 3480
BsiSI	CCGG	2 cut(s)	820, 3693
BslFI	GGGAC	2 cut(s)	2948, 3422
BslI	CCNNNNNNNGG	6 cut(s)	168, 1303, 1346, 2359, 2424, 3673
BsmAI	GTCTC	7 cut(s)	178, 427, 450, 1771, 2215, 3587, 3701
BsmFI	GGGAC	2 cut(s)	2948, 3422
BsmI	GAATGC	1 cut(s)	3784
BsnI	GGCC	9 cut(s)	234, 684, 1269, 1539, 1607, 2712, 3485, 3513, 3590
Bso31I	GGTCTC	3 cut(s)	178, 3587, 3701
BsoBI	CYCGRG	2 cut(s)	2547, 3480
Bsp119I	TTCGAA	1 cut(s)	1471
Bsp1286I	GDGCHC	1 cut(s)	953
Bsp1407I	TGTACA	3 cut(s)	2723, 2943, 3058
Bsp143I	GATC	8 cut(s)	178, 198, 203, 924, 1493, 1894, 2768, 3089
Bsp1720I	GCTNAGC	1 cut(s)	1375
Bsp19I	CCATGG	2 cut(s)	9, 1969
BspANI	GGCC	9 cut(s)	234, 684, 1269, 1539, 1607, 2712, 3485, 3513, 3590
BspCNI	CTCAG	5 cut(s)	1111, 1145, 1858, 2898, 3513
BspFNI	CGCG	1 cut(s)	1396
BspHI	TCATGA	1 cut(s)	1897
BspLI	GGNNCC	5 cut(s)	85, 561, 894, 950, 3033
BspMAI	CTGCAG	4 cut(s)	1516, 1651, 1717, 2038
BspMI	ACCTGC	2 cut(s)	3295, 3641
BspPI	GGATC	4 cut(s)	173, 193, 198, 1501
BspQI	GCTCTTC	1 cut(s)	1630
BspT104I	TTCGAA	1 cut(s)	1471
BspT107I	GGYRCC	2 cut(s)	83, 3031
BspTI	CTTAAG	1 cut(s)	2636
BspTNI	GGTCTC	3 cut(s)	178, 3587, 3701
BsrBI	CCGCTC	2 cut(s)	188, 291
BsrDI	GCAATG	5 cut(s)	57, 591, 943, 953, 3432
BsrFI	RCCGGY	1 cut(s)	3692
BsrGI	TGTACA	3 cut(s)	2723, 2943, 3058
BssAI	RCCGGY	1 cut(s)	3692
BssECI	CCNNGG	6 cut(s)	9, 87, 1881, 1969, 2577, 3597
BssMI	GATC	8 cut(s)	178, 198, 203, 924, 1493, 1894, 2768, 3089
BssSI	CACGAG	3 cut(s)	551, 1737, 2499
BssT1I	CCWWGG	4 cut(s)	9, 87, 1969, 3597
Bst2BI	CACGAG	3 cut(s)	551, 1737, 2499
Bst2UI	CCWGG	4 cut(s)	254, 1341, 1883, 2336
Bst4CI	ACNGT	8 cut(s)	249, 379, 580, 605, 1237, 1603, 1619, 3258
Bst6I	CTCTTC	4 cut(s)	1185, 1468, 1630, 2365
BstAFI	CTTAAG	1 cut(s)	2636
BstAPI	GCANNNNNTGC	5 cut(s)	848, 1646, 1916, 2275, 3434
BstAUI	TGTACA	3 cut(s)	2723, 2943, 3058
BstBI	TTCGAA	1 cut(s)	1471
BstDEI	CTNAG	8 cut(s)	145, 1119, 1132, 1375, 1866, 2906, 3500, 3525
BstDSI	CCRYGG	3 cut(s)	9, 1969, 2577
BstENI	CCTNNNNNAGG	1 cut(s)	1301
BstF5I	GGATG	7 cut(s)	328, 1177, 1513, 1813, 2251, 2437, 3751
BstFNI	CGCG	1 cut(s)	1396
BstH2I	RGCGCY	3 cut(s)	1033, 2390, 3614
BstHHI	GCGC	6 cut(s)	963, 1032, 1396, 1968, 2389, 3613
BstKTI	GATC	8 cut(s)	181, 201, 206, 927, 1496, 1897, 2771, 3092
BstMAI	GTCTC	7 cut(s)	178, 427, 450, 1771, 2215, 3587, 3701
BstMBI	GATC	8 cut(s)	178, 198, 203, 924, 1493, 1894, 2768, 3089
BstMCI	CGRYCG	1 cut(s)	1618
BstNI	CCWGG	4 cut(s)	254, 1341, 1883, 2336
BstNSI	RCATGY	4 cut(s)	742, 1966, 2593, 3241
BstPAI	GACNNNNGTC	1 cut(s)	2524
BstSCI	CCNGG	4 cut(s)	252, 1339, 1881, 2334
BstSFI	CTRYAG	8 cut(s)	834, 1512, 1647, 1713, 2034, 2886, 2930, 3768
BstUI	CGCG	1 cut(s)	1396
BstV2I	GAAGAC	3 cut(s)	72, 3201, 3302
BstX2I	RGATCY	2 cut(s)	198, 1493
BstXI	CCANNNNNNTGG	1 cut(s)	992
BstYI	RGATCY	2 cut(s)	198, 1493
BsuI	GTATCC	1 cut(s)	380
BsuRI	GGCC	9 cut(s)	234, 684, 1269, 1539, 1607, 2712, 3485, 3513, 3590
BtgI	CCRYGG	3 cut(s)	9, 1969, 2577
BtgZI	GCGATG	1 cut(s)	3500
BtsCI	GGATG	7 cut(s)	328, 1177, 1513, 1813, 2251, 2437, 3751
BtsI	GCAGTG	4 cut(s)	485, 1454, 1710, 2168
BveI	ACCTGC	2 cut(s)	3295, 3641
CaiI	CAGNNNCTG	1 cut(s)	1646
CciI	TCATGA	1 cut(s)	1897
CfoI	GCGC	6 cut(s)	963, 1032, 1396, 1968, 2389, 3613
Cfr10I	RCCGGY	1 cut(s)	3692
Cfr13I	GGNCC	8 cut(s)	232, 683, 1309, 1478, 1576, 2338, 3210, 3483
CseI	GACGC	2 cut(s)	479, 680
CspCI	CAANNNNNGTGG	2 cut(s)	847, 882
DdeI	CTNAG	8 cut(s)	145, 1119, 1132, 1375, 1866, 2906, 3500, 3525
DpnI	GATC	8 cut(s)	180, 200, 205, 926, 1495, 1896, 2770, 3091
DpnII	GATC	8 cut(s)	178, 198, 203, 924, 1493, 1894, 2768, 3089
DraI	TTTAAA	2 cut(s)	1113, 3021
EaeI	YGGCCR	4 cut(s)	1267, 1537, 3511, 3588
Eam1104I	CTCTTC	4 cut(s)	1185, 1468, 1630, 2365
EarI	CTCTTC	4 cut(s)	1185, 1468, 1630, 2365
EciI	GGCGGA	2 cut(s)	32, 886
Eco130I	CCWWGG	4 cut(s)	9, 87, 1969, 3597
Eco24I	GRGCYC	1 cut(s)	953
Eco31I	GGTCTC	3 cut(s)	178, 3587, 3701
Eco32I	GATATC	1 cut(s)	571
Eco47I	GGWCC	5 cut(s)	1309, 1478, 1576, 2338, 3210
Eco47III	AGCGCT	2 cut(s)	2388, 3612
Eco57I	CTGAAG	4 cut(s)	2285, 2462, 2514, 3441
Eco88I	CYCGRG	2 cut(s)	2547, 3480
EcoNI	CCTNNNNNAGG	1 cut(s)	1301
EcoO109I	RGGNCCY	2 cut(s)	683, 1309
EcoRII	CCWGG	4 cut(s)	252, 1339, 1881, 2334
EcoRV	GATATC	1 cut(s)	571
EcoT14I	CCWWGG	4 cut(s)	9, 87, 1969, 3597
EcoT22I	ATGCAT	2 cut(s)	356, 3784
EcoT38I	GRGCYC	1 cut(s)	953
ErhI	CCWWGG	4 cut(s)	9, 87, 1969, 3597
FalI	AAGNNNNNCTT	8 cut(s)	128, 160, 654, 686, 2073, 2105, 3543, 3575
FaqI	GGGAC	2 cut(s)	2948, 3422
FauI	CCCGC	4 cut(s)	162, 2099, 2141, 2973
FbaI	TGATCA	2 cut(s)	924, 1894
FokI	GGATG	7 cut(s)	335, 1184, 1520, 1820, 2258, 2444, 3758
FriOI	GRGCYC	1 cut(s)	953
FspBI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 2390, 3723
FspI	TGCGCA	1 cut(s)	962
GlaI	GCGC	6 cut(s)	962, 1031, 1395, 1967, 2388, 3612
GsaI	CCCAGC	3 cut(s)	1992, 2298, 3640
GsuI	CTGGAG	3 cut(s)	275, 1323, 2813
HaeII	RGCGCY	3 cut(s)	1033, 2390, 3614
HaeIII	GGCC	9 cut(s)	234, 684, 1269, 1539, 1607, 2712, 3485, 3513, 3590
HapII	CCGG	2 cut(s)	820, 3693
HgaI	GACGC	2 cut(s)	479, 680
HhaI	GCGC	6 cut(s)	963, 1032, 1396, 1968, 2389, 3613
Hin6I	GCGC	6 cut(s)	961, 1030, 1394, 1966, 2387, 3611
HinP1I	GCGC	6 cut(s)	961, 1030, 1394, 1966, 2387, 3611
HincII	GTYRAC	1 cut(s)	910
HindII	GTYRAC	1 cut(s)	910
HindIII	AAGCTT	3 cut(s)	719, 2444, 3537
HpaII	CCGG	2 cut(s)	820, 3693
HphI	GGTGA	6 cut(s)	68, 1361, 2327, 2597, 2684, 3656
Hpy166II	GTNNAC	7 cut(s)	910, 1019, 1481, 1708, 1744, 2408, 3556
Hpy8I	GTNNAC	7 cut(s)	910, 1019, 1481, 1708, 1744, 2408, 3556
Hpy99I	CGWCG	2 cut(s)	74, 77
HpyCH4III	ACNGT	8 cut(s)	249, 379, 580, 605, 1237, 1603, 1619, 3258
HpyCH4IV	ACGT	2 cut(s)	2050, 2188
HpyF3I	CTNAG	8 cut(s)	145, 1119, 1132, 1375, 1866, 2906, 3500, 3525
HpySE526I	ACGT	2 cut(s)	2050, 2188
HspAI	GCGC	6 cut(s)	961, 1030, 1394, 1966, 2387, 3611
Ksp22I	TGATCA	2 cut(s)	924, 1894
Kzo9I	GATC	8 cut(s)	178, 198, 203, 924, 1493, 1894, 2768, 3089
LguI	GCTCTTC	1 cut(s)	1630
LmnI	GCTCC	7 cut(s)	193, 634, 830, 948, 1327, 1973, 3011
MaeI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 2390, 3723
MaeII	ACGT	2 cut(s)	2050, 2188
MalI	GATC	8 cut(s)	180, 200, 205, 926, 1495, 1896, 2770, 3091
MbiI	CCGCTC	2 cut(s)	188, 291
MboI	GATC	8 cut(s)	178, 198, 203, 924, 1493, 1894, 2768, 3089
MflI	RGATCY	2 cut(s)	198, 1493
MhlI	GDGCHC	1 cut(s)	953
MlsI	TGGCCA	1 cut(s)	3513
MluNI	TGGCCA	1 cut(s)	3513
MlyI	GAGTC	6 cut(s)	2511, 2787, 3285, 3371, 3476, 3812
MmeI	TCCRAC	7 cut(s)	1152, 1305, 1648, 2284, 2970, 3277, 3654
Mox20I	TGGCCA	1 cut(s)	3513
Mph1103I	ATGCAT	2 cut(s)	356, 3784
MscI	TGGCCA	1 cut(s)	3513
MslI	CAYNNNNRTG	4 cut(s)	1024, 1238, 1559, 2016
Msp20I	TGGCCA	1 cut(s)	3513
MspA1I	CMGCKG	5 cut(s)	1643, 1988, 2126, 2824, 2918
MspCI	CTTAAG	1 cut(s)	2636
MspI	CCGG	2 cut(s)	820, 3693
MspR9I	CCNGG	4 cut(s)	254, 1341, 1883, 2336
Mva1269I	GAATGC	1 cut(s)	3784
MvaI	CCWGG	4 cut(s)	254, 1341, 1883, 2336
MvnI	CGCG	1 cut(s)	1396
NcoI	CCATGG	2 cut(s)	9, 1969
NdeII	GATC	8 cut(s)	178, 198, 203, 924, 1493, 1894, 2768, 3089
NlaIV	GGNNCC	5 cut(s)	85, 561, 894, 950, 3033
NmeAIII	GCCGAG	2 cut(s)	3616, 3755
NmuCI	GTSAC	7 cut(s)	373, 1832, 2166, 2348, 2585, 3269, 3644
NsbI	TGCGCA	1 cut(s)	962
NsiI	ATGCAT	2 cut(s)	356, 3784
NspI	RCATGY	4 cut(s)	742, 1966, 2593, 3241
NspV	TTCGAA	1 cut(s)	1471
OliI	CACNNNNGTG	2 cut(s)	1238, 2016
PaeI	GCATGC	1 cut(s)	1966
PagI	TCATGA	1 cut(s)	1897
PciI	ACATGT	1 cut(s)	2589
PciSI	GCTCTTC	1 cut(s)	1630
PcsI	WCGNNNNNNNCGW	1 cut(s)	2092
PctI	GAATGC	1 cut(s)	3784
PfeI	GAWTC	5 cut(s)	1124, 2747, 2783, 3307, 3734
PflFI	GACNNNGTC	1 cut(s)	3275
PleI	GAGTC	6 cut(s)	2510, 2786, 3284, 3371, 3475, 3811
PpsI	GAGTC	6 cut(s)	2510, 2786, 3284, 3371, 3475, 3811
PpuMI	RGGWCCY	1 cut(s)	1309
PscI	ACATGT	1 cut(s)	2589
PshAI	GACNNNNGTC	1 cut(s)	2524
Psp5II	RGGWCCY	1 cut(s)	1309
Psp6I	CCWGG	4 cut(s)	252, 1339, 1881, 2334
PspFI	CCCAGC	3 cut(s)	1988, 2294, 3636
PspGI	CCWGG	4 cut(s)	252, 1339, 1881, 2334
PspN4I	GGNNCC	5 cut(s)	85, 561, 894, 950, 3033
PspPI	GGNCC	8 cut(s)	232, 683, 1309, 1478, 1576, 2338, 3210, 3483
PspPPI	RGGWCCY	1 cut(s)	1309
PstI	CTGCAG	4 cut(s)	1516, 1651, 1717, 2038
PstNI	CAGNNNCTG	1 cut(s)	1646
PsuI	RGATCY	2 cut(s)	198, 1493
PsyI	GACNNNGTC	1 cut(s)	3275
PvuII	CAGCTG	5 cut(s)	1643, 1988, 2126, 2824, 2918
RseI	CAYNNNNRTG	4 cut(s)	1024, 1238, 1559, 2016
SapI	GCTCTTC	1 cut(s)	1630
Sau3AI	GATC	8 cut(s)	178, 198, 203, 924, 1493, 1894, 2768, 3089
Sau96I	GGNCC	8 cut(s)	232, 683, 1309, 1478, 1576, 2338, 3210, 3483
ScaI	AGTACT	3 cut(s)	99, 110, 783
SchI	GAGTC	6 cut(s)	2511, 2787, 3285, 3371, 3476, 3812
ScrFI	CCNGG	4 cut(s)	254, 1341, 1883, 2336
SduI	GDGCHC	1 cut(s)	953
SfcI	CTRYAG	8 cut(s)	834, 1512, 1647, 1713, 2034, 2886, 2930, 3768
SfuI	TTCGAA	1 cut(s)	1471
SinI	GGWCC	5 cut(s)	1309, 1478, 1576, 2338, 3210
SmiMI	CAYNNNNRTG	4 cut(s)	1024, 1238, 1559, 2016
SmlI	CTYRAG	4 cut(s)	620, 2344, 2636, 2714
SmoI	CTYRAG	4 cut(s)	620, 2344, 2636, 2714
SphI	GCATGC	1 cut(s)	1966
SspMI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 2390, 3723
StyD4I	CCNGG	4 cut(s)	252, 1339, 1881, 2334
StyI	CCWWGG	4 cut(s)	9, 87, 1969, 3597
TaaI	ACNGT	8 cut(s)	249, 379, 580, 605, 1237, 1603, 1619, 3258
TaiI	ACGT	2 cut(s)	2053, 2191
TaqI	TCGA	7 cut(s)	716, 1096, 1471, 2776, 2781, 3699, 3737
TatI	WGTACW	9 cut(s)	97, 108, 500, 781, 2723, 2943, 3058, 3258, 3362
TauI	GCSGC	3 cut(s)	17, 1272, 1865
TfiI	GAWTC	5 cut(s)	1124, 2747, 2783, 3307, 3734
TseFI	GTSAC	7 cut(s)	373, 1832, 2166, 2348, 2585, 3269, 3644
Tsp45I	GTSAC	7 cut(s)	373, 1832, 2166, 2348, 2585, 3269, 3644
TspDTI	ATGAA	9 cut(s)	339, 989, 1014, 1176, 1269, 1745, 2128, 2720, 3033
TspGWI	ACGGA	5 cut(s)	552, 1244, 1571, 3024, 3547
Tth111I	GACNNNGTC	1 cut(s)	3275
Vha464I	CTTAAG	1 cut(s)	2636
VpaK11BI	GGWCC	5 cut(s)	1309, 1478, 1576, 2338, 3210
XagI	CCTNNNNNAGG	1 cut(s)	1301
XapI	RAATTY	6 cut(s)	327, 676, 1114, 1827, 2215, 2238
XceI	RCATGY	4 cut(s)	742, 1966, 2593, 3241
XspI	CTAG	7 cut(s)	239, 588, 933, 945, 2384, 2390, 3723
ZrmI	AGTACT	3 cut(s)	99, 110, 783
Zsp2I	ATGCAT	2 cut(s)	356, 3784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.