MD03G1004200.v1.1

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009317
Physical Location & Seq
Forward (+)
332547 .. 334648
2102 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1004200.v1.1.491

Sequence Viewer

Length: 555 bp
ATGCAGACCACAAACACGTGTCTCACCGGAAGAACTCTGCTTCGTTTTCCCTGGTCCGCCATTCAACACCGTGGAGCCGTTGTCCAGGGCGCTGATGTGTTAAAGGCGGAGCATTCACATGAAGGAAGGGGAAAAGCGGTTGTTAAGGTGGAGGTTCGTGATCTTGACTCGGGGAACAAGACATCCCTACGATTGTCGACGGATGAGCAACTCGATAAGGAAATGAAAGTGACAGTGGAGCTGTACAACGATAAACCTTTATCTGTTAAAGTTCCAAAACACGTTACGTGCGTTGTCAAGGAAGCACAAACTCCTGTGAGGGGGACTGCACAAGCACCTAAGTATGTTTTCATTATGAAATATTATTGCTTGTTCAGACTTCATATCACTACTTCTTGTTTTCTCTTCCAGTATCTGTATTTATGGAAGTTGGTACATTGTGAGGAAATATTAGCCGTGATGGAACAGGGCTTCACTGTCAAAGTACCACCTCACATCGTAGAAGGTGAAGCTGTGGTGGTTGACACCGGTGACGACTCTTATGTTAAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFP EFP_N Elong-fact-P_C

Protein Analysis

185

Amino Acids

20.84

Weight (kDa)

8.55

Isoelectric Point (pI)

23.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFP_N PF08207 33 - 71 3.1e-07 Elongation factor P (EF-P) KOW-like domain
Elong-fact-P_C PF09285 152 - 184 1.9e-09 Elongation factor P, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 197
AciI CCGC 3 cut(s) 57, 107, 137
AcvI CACGTG 1 cut(s) 18
AfaI GTAC 3 cut(s) 245, 435, 486
AfiI CCNNNNNNNGG 1 cut(s) 320
AflIII ACRYGT 3 cut(s) 15, 17, 280
AgeI ACCGGT 1 cut(s) 527
AgsI TTSAA 1 cut(s) 65
AjnI CCWGG 2 cut(s) 50, 84
AloI GAACNNNNNNTCC 2 cut(s) 167, 199
AluBI AGCT 2 cut(s) 241, 512
AluI AGCT 2 cut(s) 241, 512
Alw26I GTCTC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 415
Ama87I CYCGRG 1 cut(s) 169
AsiGI ACCGGT 1 cut(s) 527
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 3 cut(s) 16, 518, 542
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 54
BbrPI CACGTG 1 cut(s) 18
BccI CCATC 1 cut(s) 454
BceAI ACGGC 2 cut(s) 62, 440
BciT130I CCWGG 2 cut(s) 52, 86
BcoDI GTCTC 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 93
Bme1390I CCNGG 2 cut(s) 52, 86
Bme18I GGWCC 1 cut(s) 54
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 2 cut(s) 52, 86
BsaAI YACGTR 2 cut(s) 18, 288
BsaJI CCNNGG 3 cut(s) 50, 70, 85
BsaWI WCCGGW 2 cut(s) 26, 527
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 1 cut(s) 409
BseBI CCWGG 2 cut(s) 52, 86
BseDI CCNNGG 3 cut(s) 50, 70, 85
BseGI GGATG 2 cut(s) 182, 208
BseLI CCNNNNNNNGG 1 cut(s) 320
BseNI ACTGG 1 cut(s) 409
BsgI GTGCAG 1 cut(s) 312
BshTI ACCGGT 1 cut(s) 527
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 2 cut(s) 27, 528
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 1 cut(s) 320
BsmAI GTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 337
BsmI GAATGC 1 cut(s) 112
BsoBI CYCGRG 1 cut(s) 169
Bsp1407I TGTACA 1 cut(s) 243
Bsp143I GATC 1 cut(s) 160
BspACI CCGC 3 cut(s) 57, 107, 137
BspLI GGNNCC 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 527
BsrGI TGTACA 1 cut(s) 243
BsrI ACTGG 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 527
BssECI CCNNGG 3 cut(s) 50, 70, 85
BssMI GATC 1 cut(s) 160
Bst2UI CCWGG 2 cut(s) 52, 86
Bst4CI ACNGT 3 cut(s) 71, 235, 478
Bst6I CTCTTC 1 cut(s) 410
BstAUI TGTACA 1 cut(s) 243
BstBAI YACGTR 2 cut(s) 18, 288
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 2 cut(s) 182, 208
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 1 cut(s) 163
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 1 cut(s) 160
BstNI CCWGG 2 cut(s) 52, 86
BstSCI CCNGG 2 cut(s) 50, 84
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 2 cut(s) 182, 208
BtsIMutI CAGTG 2 cut(s) 240, 474
CaiI CAGNNNCTG 1 cut(s) 415
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 527
Cfr13I GGNCC 1 cut(s) 54
Csp6I GTAC 3 cut(s) 244, 434, 485
CspAI ACCGGT 1 cut(s) 527
CviAII CATG 1 cut(s) 119
CviJI RGCY 5 cut(s) 77, 241, 455, 471, 512
CviKI_1 RGCY 5 cut(s) 77, 241, 455, 471, 512
CviQI GTAC 3 cut(s) 244, 434, 485
DdeI CTNAG 1 cut(s) 339
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
EciI GGCGGA 2 cut(s) 46, 122
Eco47I GGWCC 1 cut(s) 54
Eco72I CACGTG 1 cut(s) 18
Eco88I CYCGRG 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 50, 84
FaeI CATG 1 cut(s) 122
FaiI YATR 6 cut(s) 120, 345, 356, 384, 424, 543
FaqI GGGAC 1 cut(s) 337
FatI CATG 1 cut(s) 118
FblI GTMKAC 1 cut(s) 197
FokI GGATG 2 cut(s) 169, 215
GlaI GCGC 1 cut(s) 91
HaeII RGCGCY 1 cut(s) 93
HapII CCGG 2 cut(s) 27, 528
HhaI GCGC 1 cut(s) 92
Hin1II CATG 1 cut(s) 122
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 2 cut(s) 198, 523
HindII GTYRAC 2 cut(s) 198, 523
HinfI GANTC 2 cut(s) 167, 536
HpaII CCGG 2 cut(s) 27, 528
HphI GGTGA 3 cut(s) 16, 518, 542
Hpy166II GTNNAC 2 cut(s) 198, 523
Hpy188I TCNGA 1 cut(s) 377
Hpy188III TCNNGA 2 cut(s) 158, 164
Hpy8I GTNNAC 2 cut(s) 198, 523
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 3 cut(s) 116, 120, 497
HpyCH4III ACNGT 3 cut(s) 71, 235, 478
HpyCH4IV ACGT 3 cut(s) 17, 282, 287
HpyCH4V TGCA 2 cut(s) 4, 329
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 3 cut(s) 17, 282, 287
Hsp92II CATG 1 cut(s) 122
HspAI GCGC 1 cut(s) 90
Kzo9I GATC 1 cut(s) 160
LmnI GCTCC 3 cut(s) 74, 109, 238
LpnPI CCDG 9 cut(s) 37, 40, 64, 71, 98, 327, 422, 452, 541
MaeII ACGT 3 cut(s) 17, 282, 287
MaeIII GTNAC 3 cut(s) 229, 283, 530
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 2 cut(s) 42, 397
MlyI GAGTC 2 cut(s) 161, 530
MnlI CCTC 4 cut(s) 145, 312, 436, 501
MseI TTAA 4 cut(s) 101, 144, 267, 546
MslI CAYNNNNRTG 1 cut(s) 117
MspI CCGG 2 cut(s) 27, 528
MspR9I CCNGG 2 cut(s) 52, 86
Mva1269I GAATGC 1 cut(s) 112
MvaI CCWGG 2 cut(s) 52, 86
NdeII GATC 1 cut(s) 160
NlaIII CATG 1 cut(s) 122
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 229, 530
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PctI GAATGC 1 cut(s) 112
PinAI ACCGGT 1 cut(s) 527
PleI GAGTC 2 cut(s) 161, 530
PmaCI CACGTG 1 cut(s) 18
PmlI CACGTG 1 cut(s) 18
PpsI GAGTC 2 cut(s) 161, 530
Ppu21I YACGTR 2 cut(s) 18, 288
Psp6I CCWGG 2 cut(s) 50, 84
PspCI CACGTG 1 cut(s) 18
PspGI CCWGG 2 cut(s) 50, 84
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 415
RsaI GTAC 3 cut(s) 245, 435, 486
RsaNI GTAC 3 cut(s) 244, 434, 485
RseI CAYNNNNRTG 1 cut(s) 117
SalI GTCGAC 1 cut(s) 196
SaqAI TTAA 4 cut(s) 101, 144, 267, 546
Sau3AI GATC 1 cut(s) 160
Sau96I GGNCC 1 cut(s) 54
SchI GAGTC 2 cut(s) 161, 530
ScrFI CCNGG 2 cut(s) 52, 86
SgrAI CRCCGGYG 1 cut(s) 527
SinI GGWCC 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 117
SsiI CCGC 3 cut(s) 57, 107, 137
SspI AATATT 2 cut(s) 362, 450
StyD4I CCNGG 2 cut(s) 50, 84
TaaI ACNGT 3 cut(s) 71, 235, 478
TaiI ACGT 3 cut(s) 20, 285, 290
TaqI TCGA 2 cut(s) 197, 213
TatI WGTACW 1 cut(s) 243
Tru1I TTAA 4 cut(s) 101, 144, 267, 546
Tru9I TTAA 4 cut(s) 101, 144, 267, 546
TscAI CASTG 2 cut(s) 240, 481
TseFI GTSAC 2 cut(s) 229, 530
Tsp45I GTSAC 2 cut(s) 229, 530
TspDTI ATGAA 5 cut(s) 135, 239, 340, 371, 371
TspGWI ACGGA 1 cut(s) 215
TspRI CASTG 2 cut(s) 240, 481
VpaK11BI GGWCC 1 cut(s) 54
XmiI GTMKAC 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.