Rh5AG538500

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009317
Physical Location & Seq
Reverse (-)
92649206 .. 92653059
3854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG538500.1

Sequence Viewer

Length: 780 bp
ATGCAAGCGGTGGCTGCCAAACTCACTCGCCCTCACTTCCTCACCAGAACCTTAACCACCCTCACTCTCTCTTCTTCTTCTCCTCTCTCCAATTTGCTATCCTCCCCCGGCCGTGACGCCGCCACCATCACCCACGCCACCAACACGTGTCTCACCCGCCGAACATCTCTCCTCTCATCTCCTCCTTGGTCCGCCACTCAACACCGCGGCGCCAGAGTCCAAGGCGCCGATGTGAGACCTGGCAATGTGATTCAAAAGAAAGATCGTATTTACCAGGTGATAAAGGTAGAGCATTCACATGAAGGAAGAGGAAAGGCCAACATTAAGCTGGAGCTTCGTGATGTCGACTCTGGGAACAAGACATCCCAAAGATTGGGGACAGAGGAGTCAGTTGATAGAGTATTTGTTATGTCAAAAAGTTACATTTACATGTGCACAGATCGGGATGGCAAAGTATTATTGATGGATCCTGATACGCTTGATCAGCTCGAAGTGAATGAAGACGTATTTGGAAAGAATGCTAAATACCTACAAGAAGAAATGAAAGTTAGAGTGGAGCTGTTTAACGGGACCCCTTTATCTGCATCAGTTCCAAAACATGTGACCTGCACTGTCAAGGAAGCACAACCTCCTATAAAGGGGATTGCAGCAACACCTAAGGATAAAATAGCTACAATCCAAAATGGCTTCACTATAAAAGTACCTGCTCATATCATAGCTGGTGAGTCTGTGGTTATTGACACCGAGGATGACTCTTATGTTAGAAGGGCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFP EFP_N Elong-fact-P_C

Protein Analysis

259

Amino Acids

28.48

Weight (kDa)

9.3

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFP_N PF08207 76 - 131 1.5e-12 Elongation factor P (EF-P) KOW-like domain
EFP PF01132 151 - 193 9.3e-12 Elongation factor P (EF-P) OB domain
Elong-fact-P_C PF09285 201 - 256 1.5e-15 Elongation factor P, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 385
Acc36I ACCTGC 2 cut(s) 614, 712
AccB1I GGYRCC 2 cut(s) 209, 224
AccB7I CCANNNNNTGG 1 cut(s) 373
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 207
AciI CCGC 6 cut(s) 8, 120, 157, 192, 205, 207
AclWI GGATC 2 cut(s) 461, 474
AcoI YGGCCR 1 cut(s) 109
AcvI CACGTG 1 cut(s) 147
AcyI GRCGYC 3 cut(s) 117, 210, 225
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 373, 638
AflIII ACRYGT 4 cut(s) 144, 146, 429, 598
AgsI TTSAA 1 cut(s) 254
AjnI CCWGG 2 cut(s) 238, 273
AjuI GAANNNNNNNTTGG 2 cut(s) 492, 524
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
AluBI AGCT 6 cut(s) 328, 334, 487, 559, 671, 719
AluI AGCT 6 cut(s) 328, 334, 487, 559, 671, 719
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 2 cut(s) 155, 229
Alw44I GTGCAC 1 cut(s) 433
AlwI GGATC 2 cut(s) 461, 474
AoxI GGCC 3 cut(s) 109, 315, 768
ApaLI GTGCAC 1 cut(s) 433
ApeKI GCWGC 2 cut(s) 14, 647
AspLEI GCGC 2 cut(s) 212, 227
AspS9I GGNCC 3 cut(s) 189, 570, 768
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 5 cut(s) 34, 121, 145, 289, 734
AvaII GGWCC 2 cut(s) 189, 570
AxyI CCTNAGG 1 cut(s) 657
BaeGI GKGCMC 1 cut(s) 437
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 2 cut(s) 209, 224
BbrPI CACGTG 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 1 cut(s) 659
BccI CCATC 3 cut(s) 134, 440, 457
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 2 cut(s) 240, 275
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 2 cut(s) 155, 229
BfoI RGCGCY 2 cut(s) 213, 228
BfuAI ACCTGC 2 cut(s) 614, 712
BisI GCNGC 4 cut(s) 15, 120, 208, 648
BlsI GCNGC 4 cut(s) 16, 121, 209, 649
Bme1390I CCNGG 3 cut(s) 108, 240, 275
Bme18I GGWCC 2 cut(s) 189, 570
BmgT120I GGNCC 3 cut(s) 189, 570, 768
BmiI GGNNCC 5 cut(s) 211, 226, 468, 571, 572
BmrFI CCNGG 3 cut(s) 108, 240, 275
BmsI GCATC 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 507
BplI GAGNNNNNCTC 2 cut(s) 737, 769
BpmI CTGGAG 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 108
BsaAI YACGTR 1 cut(s) 147
BsaHI GRCGYC 3 cut(s) 117, 210, 225
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 6 cut(s) 106, 185, 205, 220, 744, 771
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 638
Bse21I CCTNAGG 1 cut(s) 657
Bse3DI GCAATG 1 cut(s) 250
BseBI CCWGG 2 cut(s) 240, 275
BseDI CCNNGG 6 cut(s) 106, 185, 205, 220, 744, 771
BseGI GGATG 3 cut(s) 362, 451, 754
BseLI CCNNNNNNNGG 2 cut(s) 373, 638
BseMI GCAATG 1 cut(s) 250
BseRI GAGGAG 4 cut(s) 72, 161, 171, 398
BseSI GKGCMC 1 cut(s) 437
BseX3I CGGCCG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 659
BsgI GTGCAG 1 cut(s) 592
Bsh1236I CGCG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 3 cut(s) 111, 317, 770
BshNI GGYRCC 2 cut(s) 209, 224
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 2 cut(s) 391, 583
BslI CCNNNNNNNGG 2 cut(s) 373, 638
BsmAI GTCTC 2 cut(s) 155, 229
BsmFI GGGAC 2 cut(s) 391, 583
BsmI GAATGC 2 cut(s) 292, 523
BsnI GGCC 3 cut(s) 111, 317, 770
Bso31I GGTCTC 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 437
Bsp143I GATC 4 cut(s) 262, 439, 466, 481
BspACI CCGC 6 cut(s) 8, 120, 157, 192, 205, 207
BspANI GGCC 3 cut(s) 111, 317, 770
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 5 cut(s) 211, 226, 468, 571, 572
BspMI ACCTGC 2 cut(s) 614, 712
BspPI GGATC 2 cut(s) 461, 474
BspT107I GGYRCC 2 cut(s) 209, 224
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 250
BssECI CCNNGG 6 cut(s) 106, 185, 205, 220, 744, 771
BssMI GATC 4 cut(s) 262, 439, 466, 481
BssNI GRCGYC 3 cut(s) 117, 210, 225
BssT1I CCWWGG 3 cut(s) 185, 220, 771
Bst2UI CCWGG 2 cut(s) 240, 275
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 2 cut(s) 76, 301
BstACI GRCGYC 3 cut(s) 117, 210, 225
BstBAI YACGTR 1 cut(s) 147
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 657
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 3 cut(s) 362, 451, 754
BstFNI CGCG 1 cut(s) 207
BstH2I RGCGCY 2 cut(s) 213, 228
BstHHI GCGC 2 cut(s) 212, 227
BstKTI GATC 4 cut(s) 265, 442, 469, 484
BstMAI GTCTC 2 cut(s) 155, 229
BstMBI GATC 4 cut(s) 262, 439, 466, 481
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 2 cut(s) 14, 484
BstNI CCWGG 2 cut(s) 240, 275
BstNSI RCATGY 2 cut(s) 433, 602
BstSCI CCNGG 3 cut(s) 106, 238, 273
BstSLI GKGCMC 1 cut(s) 437
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZI CGGCCG 1 cut(s) 109
Bsu36I CCTNAGG 1 cut(s) 657
BsuRI GGCC 3 cut(s) 111, 317, 770
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 3 cut(s) 362, 451, 754
BtsIMutI CAGTG 1 cut(s) 609
BveI ACCTGC 2 cut(s) 614, 712
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 212, 227
Cfr13I GGNCC 3 cut(s) 189, 570, 768
Cfr42I CCGCGG 1 cut(s) 208
CseI GACGC 1 cut(s) 125
CsiI ACCWGGT 1 cut(s) 273
Csp6I GTAC 1 cut(s) 701
CviAII CATG 3 cut(s) 299, 430, 599
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 1 cut(s) 657
DinI GGCGCC 2 cut(s) 211, 226
DpnI GATC 4 cut(s) 264, 441, 468, 483
DpnII GATC 4 cut(s) 262, 439, 466, 481
DrdI GACNNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 109
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 76, 301
EarI CTCTTC 2 cut(s) 76, 301
EciI GGCGGA 1 cut(s) 181
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 3 cut(s) 185, 220, 771
Eco31I GGTCTC 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 189, 570
Eco52I CGGCCG 1 cut(s) 109
Eco72I CACGTG 1 cut(s) 147
Eco81I CCTNAGG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 570
EcoRII CCWGG 2 cut(s) 238, 273
EcoT14I CCWWGG 3 cut(s) 185, 220, 771
EgeI GGCGCC 2 cut(s) 211, 226
EheI GGCGCC 2 cut(s) 211, 226
ErhI CCWWGG 3 cut(s) 185, 220, 771
FaeI CATG 3 cut(s) 302, 433, 602
FaqI GGGAC 2 cut(s) 391, 583
FatI CATG 3 cut(s) 298, 429, 598
FauI CCCGC 1 cut(s) 164
FbaI TGATCA 1 cut(s) 481
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 15, 120, 208, 648
FokI GGATG 3 cut(s) 349, 458, 761
Fsp4HI GCNGC 4 cut(s) 15, 120, 208, 648
GlaI GCGC 2 cut(s) 211, 226
GluI GCNGC 4 cut(s) 15, 120, 208, 648
GsuI CTGGAG 1 cut(s) 350
HaeII RGCGCY 2 cut(s) 213, 228
HaeIII GGCC 3 cut(s) 111, 317, 770
HapII CCGG 1 cut(s) 108
HgaI GACGC 1 cut(s) 125
HhaI GCGC 2 cut(s) 212, 227
Hin1I GRCGYC 3 cut(s) 117, 210, 225
Hin1II CATG 3 cut(s) 302, 433, 602
Hin6I GCGC 2 cut(s) 210, 225
HinP1I GCGC 2 cut(s) 210, 225
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 6 cut(s) 216, 250, 347, 386, 725, 752
HpaII CCGG 1 cut(s) 108
HphI GGTGA 5 cut(s) 34, 121, 145, 289, 734
Hpy166II GTNNAC 2 cut(s) 346, 435
Hpy188III TCNNGA 3 cut(s) 338, 443, 470
Hpy8I GTNNAC 2 cut(s) 346, 435
HpyAV CCTTC 2 cut(s) 296, 759
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 2 cut(s) 146, 504
HpyCH4V TGCA 5 cut(s) 4, 435, 584, 609, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 484
HpyF3I CTNAG 1 cut(s) 657
HpySE526I ACGT 2 cut(s) 146, 504
Hsp92I GRCGYC 3 cut(s) 117, 210, 225
Hsp92II CATG 3 cut(s) 302, 433, 602
HspAI GCGC 2 cut(s) 210, 225
KasI GGCGCC 2 cut(s) 209, 224
KflI GGGWCCC 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 481
KspI CCGCGG 1 cut(s) 208
Kzo9I GATC 4 cut(s) 262, 439, 466, 481
LmnI GCTCC 2 cut(s) 331, 556
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 1 cut(s) 593
MabI ACCWGGT 1 cut(s) 273
MaeII ACGT 2 cut(s) 146, 504
MaeIII GTNAC 3 cut(s) 113, 419, 601
MalI GATC 4 cut(s) 264, 441, 468, 483
MboI GATC 4 cut(s) 262, 439, 466, 481
MboII GAAGA 6 cut(s) 63, 66, 69, 318, 512, 548
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 1 cut(s) 91
Mly113I GGCGCC 2 cut(s) 210, 225
MlyI GAGTC 5 cut(s) 225, 341, 395, 734, 746
MseI TTAA 3 cut(s) 53, 324, 564
MslI CAYNNNNRTG 2 cut(s) 297, 428
MspA1I CMGCKG 1 cut(s) 207
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 3 cut(s) 108, 240, 275
Mva1269I GAATGC 2 cut(s) 292, 523
MvaI CCWGG 2 cut(s) 240, 275
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 14, 484
NarI GGCGCC 2 cut(s) 210, 225
NciI CCSGG 1 cut(s) 108
NdeII GATC 4 cut(s) 262, 439, 466, 481
NlaIII CATG 3 cut(s) 302, 433, 602
NlaIV GGNNCC 5 cut(s) 211, 226, 468, 571, 572
NmuCI GTSAC 2 cut(s) 113, 601
NspI RCATGY 2 cut(s) 433, 602
PciI ACATGT 2 cut(s) 429, 598
PctI GAATGC 2 cut(s) 292, 523
PfeI GAWTC 1 cut(s) 250
PflMI CCANNNNNTGG 1 cut(s) 373
PkrI GCNGC 4 cut(s) 16, 121, 209, 649
PleI GAGTC 5 cut(s) 224, 341, 394, 733, 746
PluTI GGCGCC 2 cut(s) 213, 228
PmaCI CACGTG 1 cut(s) 147
PmlI CACGTG 1 cut(s) 147
PpsI GAGTC 5 cut(s) 224, 341, 394, 733, 746
Ppu21I YACGTR 1 cut(s) 147
PpuMI RGGWCCY 1 cut(s) 570
PscI ACATGT 2 cut(s) 429, 598
Psp5II RGGWCCY 1 cut(s) 570
Psp6I CCWGG 2 cut(s) 238, 273
PspCI CACGTG 1 cut(s) 147
PspGI CCWGG 2 cut(s) 238, 273
PspN4I GGNNCC 5 cut(s) 211, 226, 468, 571, 572
PspPI GGNCC 3 cut(s) 189, 570, 768
PspPPI RGGWCCY 1 cut(s) 570
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
RseI CAYNNNNRTG 2 cut(s) 297, 428
SacII CCGCGG 1 cut(s) 208
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 3 cut(s) 53, 324, 564
SatI GCNGC 4 cut(s) 15, 120, 208, 648
Sau3AI GATC 4 cut(s) 262, 439, 466, 481
Sau96I GGNCC 3 cut(s) 189, 570, 768
SchI GAGTC 5 cut(s) 225, 341, 395, 734, 746
ScrFI CCNGG 3 cut(s) 108, 240, 275
SduI GDGCHC 1 cut(s) 437
SexAI ACCWGGT 1 cut(s) 273
SfaNI GCATC 1 cut(s) 593
SfoI GGCGCC 2 cut(s) 211, 226
Sfr303I CCGCGG 1 cut(s) 208
SgrBI CCGCGG 1 cut(s) 208
SinI GGWCC 2 cut(s) 189, 570
SmiMI CAYNNNNRTG 2 cut(s) 297, 428
Sse9I AATT 1 cut(s) 91
SsiI CCGC 6 cut(s) 8, 120, 157, 192, 205, 207
SspDI GGCGCC 2 cut(s) 209, 224
StyD4I CCNGG 3 cut(s) 106, 238, 273
StyI CCWWGG 3 cut(s) 185, 220, 771
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 2 cut(s) 149, 507
TaqI TCGA 2 cut(s) 345, 489
TasI AATT 1 cut(s) 91
TauI GCSGC 2 cut(s) 122, 210
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 3 cut(s) 53, 324, 564
Tru9I TTAA 3 cut(s) 53, 324, 564
TscAI CASTG 1 cut(s) 616
TseFI GTSAC 2 cut(s) 113, 601
TseI GCWGC 2 cut(s) 14, 647
Tsp45I GTSAC 2 cut(s) 113, 601
TspDTI ATGAA 3 cut(s) 315, 513, 557
TspRI CASTG 1 cut(s) 616
Van91I CCANNNNNTGG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 433
VpaK11BI GGWCC 2 cut(s) 189, 570
XceI RCATGY 2 cut(s) 433, 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.