Rh5BG563800

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009317
Physical Location & Seq
Reverse (-)
89357567 .. 89362085
4519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG563800.1

Sequence Viewer

Length: 666 bp
ATGCAAGCGGTGGCTGCCAAACTCACTCGCCCTCACTTCCTCACCAGAACCTTAACCACCCTCACTCTCTCTTCTTCTTCTACTCTCTCCAATTTGCTATCCTCCCCCGGCCGTGACGCCGCCACCATCACCCACGCGACCAACGAGTGTCTCGCCCGCCGAACATCTCTGCTCTCGTCTCCTCCTTGGTCCGCCACTCAACACCGCGGCGCCAGAGTCCAAGGCGCCGATGTGAGACCTGGCAATGTGATTCAAAAGAAAGATCGTATTTACCAGGTGATAAAGGTAGAGCATTCACATGAAGGAAGAGGAAAGGCCAACATTAAGCTGGAGCTTCGTGATGTGGACTCTGGGAATAAGACATCCCAAAGATTGGGGACAGAGGAGACAGTTGATAGAGTATTTGTCATGTCAAAAAGTTACATTTACATGTGCACAGACCGGGATGGAAAAGTATTATTGATGGATCCTGATACGCTTGATCAGCTTGAAGTGAATGAAGACGTATTTGGAAAGAATGCTAAATACCTACAAGGTCAATGCTTTTATAATACATGTTCCTTTCTGTTCGTTTGTTATTTATCATATAACTTGAACCCTGAATATTTTTTTAGTGTCTGTATTCCAAAATATATCAATAACTCATGGTTATGGAATAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFP EFP_N Elong-fact-P_C

Protein Analysis

221

Amino Acids

25.01

Weight (kDa)

9.0

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFP_N PF08207 76 - 131 1.2e-12 Elongation factor P (EF-P) KOW-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
AccB1I GGYRCC 2 cut(s) 209, 224
AccB7I CCANNNNNTGG 1 cut(s) 373
AccII CGCG 2 cut(s) 137, 207
AciI CCGC 6 cut(s) 8, 120, 157, 192, 205, 207
AclWI GGATC 2 cut(s) 461, 474
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 660
AcyI GRCGYC 3 cut(s) 117, 210, 225
AfiI CCNNNNNNNGG 1 cut(s) 373
AflIII ACRYGT 2 cut(s) 429, 554
AgsI TTSAA 3 cut(s) 254, 491, 595
AjnI CCWGG 2 cut(s) 238, 273
AjuI GAANNNNNNNTTGG 2 cut(s) 492, 524
AluBI AGCT 3 cut(s) 328, 334, 487
AluI AGCT 3 cut(s) 328, 334, 487
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 4 cut(s) 155, 183, 229, 380
Alw44I GTGCAC 1 cut(s) 433
AlwI GGATC 2 cut(s) 461, 474
AoxI GGCC 2 cut(s) 109, 315
ApaLI GTGCAC 1 cut(s) 433
ApeKI GCWGC 1 cut(s) 14
ApoI RAATTY 1 cut(s) 660
AspLEI GCGC 2 cut(s) 212, 227
AspS9I GGNCC 1 cut(s) 189
AsuC2I CCSGG 2 cut(s) 108, 443
AsuHPI GGTGA 3 cut(s) 34, 121, 289
AvaII GGWCC 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 437
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 2 cut(s) 209, 224
BbsI GAAGAC 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 437
BccI CCATC 3 cut(s) 134, 440, 457
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 2 cut(s) 240, 275
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 2 cut(s) 108, 443
BcoDI GTCTC 4 cut(s) 155, 183, 229, 380
BfoI RGCGCY 2 cut(s) 213, 228
BisI GCNGC 3 cut(s) 15, 120, 208
BlsI GCNGC 3 cut(s) 16, 121, 209
Bme1390I CCNGG 4 cut(s) 108, 240, 275, 443
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 3 cut(s) 211, 226, 468
BmrFI CCNGG 4 cut(s) 108, 240, 275, 443
BpiI GAAGAC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 350
BpuMI CCSGG 2 cut(s) 108, 443
BsaHI GRCGYC 3 cut(s) 117, 210, 225
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 4 cut(s) 106, 185, 205, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse3DI GCAATG 1 cut(s) 250
BseBI CCWGG 2 cut(s) 240, 275
BseDI CCNNGG 4 cut(s) 106, 185, 205, 220
BseGI GGATG 2 cut(s) 362, 451
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMI GCAATG 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 171, 398
BseSI GKGCMC 1 cut(s) 437
BseX3I CGGCCG 1 cut(s) 109
Bsh1236I CGCG 2 cut(s) 137, 207
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 111, 317
BshNI GGYRCC 2 cut(s) 209, 224
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 2 cut(s) 108, 442
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 4 cut(s) 155, 183, 229, 380
BsmBI CGTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 391
BsmI GAATGC 2 cut(s) 292, 523
BsnI GGCC 2 cut(s) 111, 317
Bso31I GGTCTC 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 437
Bsp143I GATC 3 cut(s) 262, 466, 481
BspACI CCGC 6 cut(s) 8, 120, 157, 192, 205, 207
BspANI GGCC 2 cut(s) 111, 317
BspFNI CGCG 2 cut(s) 137, 207
BspLI GGNNCC 3 cut(s) 211, 226, 468
BspPI GGATC 2 cut(s) 461, 474
BspT107I GGYRCC 2 cut(s) 209, 224
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 250
BssECI CCNNGG 4 cut(s) 106, 185, 205, 220
BssMI GATC 3 cut(s) 262, 466, 481
BssNI GRCGYC 3 cut(s) 117, 210, 225
BssT1I CCWWGG 2 cut(s) 185, 220
Bst2UI CCWGG 2 cut(s) 240, 275
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 2 cut(s) 76, 301
BstACI GRCGYC 3 cut(s) 117, 210, 225
BstC8I GCNNGC 2 cut(s) 6, 157
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 2 cut(s) 362, 451
BstFNI CGCG 2 cut(s) 137, 207
BstH2I RGCGCY 2 cut(s) 213, 228
BstHHI GCGC 2 cut(s) 212, 227
BstKTI GATC 3 cut(s) 265, 469, 484
BstMAI GTCTC 4 cut(s) 155, 183, 229, 380
BstMBI GATC 3 cut(s) 262, 466, 481
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 2 cut(s) 14, 484
BstNI CCWGG 2 cut(s) 240, 275
BstNSI RCATGY 2 cut(s) 433, 558
BstSCI CCNGG 4 cut(s) 106, 238, 273, 441
BstSLI GKGCMC 1 cut(s) 437
BstUI CGCG 2 cut(s) 137, 207
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZI CGGCCG 1 cut(s) 109
BsuRI GGCC 2 cut(s) 111, 317
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 2 cut(s) 362, 451
Cac8I GCNNGC 2 cut(s) 6, 157
CfoI GCGC 2 cut(s) 212, 227
Cfr13I GGNCC 1 cut(s) 189
Cfr42I CCGCGG 1 cut(s) 208
CseI GACGC 1 cut(s) 125
CsiI ACCWGGT 1 cut(s) 273
CviAII CATG 5 cut(s) 299, 409, 430, 555, 645
CviJI RGCY 6 cut(s) 14, 111, 317, 328, 334, 487
CviKI_1 RGCY 6 cut(s) 14, 111, 317, 328, 334, 487
DinI GGCGCC 2 cut(s) 211, 226
DpnI GATC 3 cut(s) 264, 468, 483
DpnII GATC 3 cut(s) 262, 466, 481
EaeI YGGCCR 1 cut(s) 109
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 76, 301
EarI CTCTTC 2 cut(s) 76, 301
EciI GGCGGA 1 cut(s) 181
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 2 cut(s) 185, 220
Eco31I GGTCTC 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 189
Eco52I CGGCCG 1 cut(s) 109
EcoRII CCWGG 2 cut(s) 238, 273
EcoT14I CCWWGG 2 cut(s) 185, 220
EgeI GGCGCC 2 cut(s) 211, 226
EheI GGCGCC 2 cut(s) 211, 226
ErhI CCWWGG 2 cut(s) 185, 220
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 5 cut(s) 302, 412, 433, 558, 648
FaqI GGGAC 1 cut(s) 391
FatI CATG 5 cut(s) 298, 408, 429, 554, 644
FauI CCCGC 1 cut(s) 164
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 3 cut(s) 15, 120, 208
FokI GGATG 2 cut(s) 349, 458
Fsp4HI GCNGC 3 cut(s) 15, 120, 208
GlaI GCGC 2 cut(s) 211, 226
GluI GCNGC 3 cut(s) 15, 120, 208
GsuI CTGGAG 1 cut(s) 350
HaeII RGCGCY 2 cut(s) 213, 228
HaeIII GGCC 2 cut(s) 111, 317
HapII CCGG 2 cut(s) 108, 442
HgaI GACGC 1 cut(s) 125
HhaI GCGC 2 cut(s) 212, 227
Hin1I GRCGYC 3 cut(s) 117, 210, 225
Hin1II CATG 5 cut(s) 302, 412, 433, 558, 648
Hin6I GCGC 2 cut(s) 210, 225
HinP1I GCGC 2 cut(s) 210, 225
HinfI GANTC 3 cut(s) 216, 250, 347
HpaII CCGG 2 cut(s) 108, 442
HphI GGTGA 3 cut(s) 34, 121, 289
Hpy166II GTNNAC 2 cut(s) 346, 435
Hpy188III TCNNGA 2 cut(s) 338, 470
Hpy8I GTNNAC 2 cut(s) 346, 435
HpyAV CCTTC 1 cut(s) 296
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 2 cut(s) 4, 435
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 484
HpySE526I ACGT 1 cut(s) 504
Hsp92I GRCGYC 3 cut(s) 117, 210, 225
Hsp92II CATG 5 cut(s) 302, 412, 433, 558, 648
HspAI GCGC 2 cut(s) 210, 225
KasI GGCGCC 2 cut(s) 209, 224
Ksp22I TGATCA 1 cut(s) 481
KspI CCGCGG 1 cut(s) 208
Kzo9I GATC 3 cut(s) 262, 466, 481
LmnI GCTCC 1 cut(s) 331
MabI ACCWGGT 1 cut(s) 273
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 2 cut(s) 113, 419
MalI GATC 3 cut(s) 264, 468, 483
MboI GATC 3 cut(s) 262, 466, 481
MboII GAAGA 5 cut(s) 63, 66, 69, 318, 512
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 2 cut(s) 91, 660
Mly113I GGCGCC 2 cut(s) 210, 225
MlyI GAGTC 2 cut(s) 225, 341
MnlI CCTC 7 cut(s) 42, 50, 71, 112, 192, 302, 376
MseI TTAA 3 cut(s) 53, 324, 664
MslI CAYNNNNRTG 3 cut(s) 297, 428, 649
MspA1I CMGCKG 1 cut(s) 207
MspI CCGG 2 cut(s) 108, 442
MspR9I CCNGG 4 cut(s) 108, 240, 275, 443
Mva1269I GAATGC 2 cut(s) 292, 523
MvaI CCWGG 2 cut(s) 240, 275
MvnI CGCG 2 cut(s) 137, 207
MwoI GCNNNNNNNGC 2 cut(s) 14, 484
NarI GGCGCC 2 cut(s) 210, 225
NciI CCSGG 2 cut(s) 108, 443
NdeII GATC 3 cut(s) 262, 466, 481
NlaIII CATG 5 cut(s) 302, 412, 433, 558, 648
NlaIV GGNNCC 3 cut(s) 211, 226, 468
NmuCI GTSAC 1 cut(s) 113
NspI RCATGY 2 cut(s) 433, 558
PciI ACATGT 2 cut(s) 429, 554
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 2 cut(s) 292, 523
PfeI GAWTC 1 cut(s) 250
PflMI CCANNNNNTGG 1 cut(s) 373
PkrI GCNGC 3 cut(s) 16, 121, 209
PleI GAGTC 2 cut(s) 224, 341
PluTI GGCGCC 2 cut(s) 213, 228
PpsI GAGTC 2 cut(s) 224, 341
PscI ACATGT 2 cut(s) 429, 554
PsiI TTATAA 1 cut(s) 549
Psp6I CCWGG 2 cut(s) 238, 273
PspGI CCWGG 2 cut(s) 238, 273
PspN4I GGNNCC 3 cut(s) 211, 226, 468
PspPI GGNCC 1 cut(s) 189
PsuI RGATCY 1 cut(s) 466
RseI CAYNNNNRTG 3 cut(s) 297, 428, 649
SacII CCGCGG 1 cut(s) 208
SaqAI TTAA 3 cut(s) 53, 324, 664
SatI GCNGC 3 cut(s) 15, 120, 208
Sau3AI GATC 3 cut(s) 262, 466, 481
Sau96I GGNCC 1 cut(s) 189
SchI GAGTC 2 cut(s) 225, 341
ScrFI CCNGG 4 cut(s) 108, 240, 275, 443
SduI GDGCHC 1 cut(s) 437
SexAI ACCWGGT 1 cut(s) 273
SfoI GGCGCC 2 cut(s) 211, 226
Sfr303I CCGCGG 1 cut(s) 208
SgrBI CCGCGG 1 cut(s) 208
SinI GGWCC 1 cut(s) 189
SmiMI CAYNNNNRTG 3 cut(s) 297, 428, 649
Sse9I AATT 2 cut(s) 91, 660
SsiI CCGC 6 cut(s) 8, 120, 157, 192, 205, 207
SspDI GGCGCC 2 cut(s) 209, 224
SspI AATATT 1 cut(s) 605
StyD4I CCNGG 4 cut(s) 106, 238, 273, 441
StyI CCWWGG 2 cut(s) 185, 220
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 507
TasI AATT 2 cut(s) 91, 660
TauI GCSGC 2 cut(s) 122, 210
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 3 cut(s) 53, 324, 664
Tru9I TTAA 3 cut(s) 53, 324, 664
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 2 cut(s) 315, 513
Van91I CCANNNNNTGG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 189
XapI RAATTY 1 cut(s) 660
XceI RCATGY 2 cut(s) 433, 558
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.