Rh5DG572100

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0009317
Physical Location & Seq
Reverse (-)
87947793 .. 87952119
4327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG572100.1

Sequence Viewer

Length: 762 bp
ATGCAAGCGGTGGCTGCCAAACTCACTCGCCCTCACTTCCTCACCAGAACCTTAACCACCCTCACTCTCTCTTCTTCTTCTCCTCTCTCCAACTTGCTATCCTCCCCCGGCCGTGACGCCGCCACCGACACGTGTCTCACCCGCCGAACATCTCTCCTCACATCTCCTCCTTGGTCCGCCACTCAACACCGCGGCGCCAGAGTCCAAGGCGCCGATGTGAGACCTGGCAATGTGATTCAAAAGAAAGATCGTATTTACCAGGTGATAAAGGTAGAGCATTCACATGAAGGAAGAGGAAAGGCCAACATTAAGCTGGAGCTTCGTGATGTCGACTCTGGGAACAAGACATCCCAAAGATTGGGGACAGAGGAGTCAGTTGATAGAGTGTTTGTCATGTCAAAAAGTTACATTTACATGTGCACAGACCGGGATGGCAAAGTATTATTGATGGATCCTGATACGCTTGATCAGCTCGAAGTGAATGAAGACGTATTTGGAAAGAATGCTAAATACCTACAAGAAGAAATGAAAGTTAGAGTGGAGCTGTTTAACGGGACCCCTTTATCTGCATCAGTTCCAAAACATGTGACCTGCACTGTCAAGGAAGCACAACCTCCTATAAAGGGGATTGCAGCAACACCTAAGGATAAAATAGCTACAATCCAAAATGGCTTCACTATAAAAGTAAGTTACATTTTTTATATAATATTTTTACACATGCTAGGTGTTTGTAGCTATTATGAGAAGAAAAATATGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFP EFP_N Elong-fact-P_C

Protein Analysis

253

Amino Acids

28.22

Weight (kDa)

9.36

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFP_N PF08207 71 - 126 1.5e-12 Elongation factor P (EF-P) KOW-like domain
EFP PF01132 146 - 188 8.9e-12 Elongation factor P (EF-P) OB domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 370
Acc36I ACCTGC 1 cut(s) 599
AccB1I GGYRCC 2 cut(s) 194, 209
AccB7I CCANNNNNTGG 1 cut(s) 358
AccI GTMKAC 1 cut(s) 330
AccII CGCG 1 cut(s) 192
AciI CCGC 6 cut(s) 8, 120, 142, 177, 190, 192
AclWI GGATC 2 cut(s) 446, 459
AcoI YGGCCR 1 cut(s) 109
AcvI CACGTG 1 cut(s) 132
AcyI GRCGYC 3 cut(s) 117, 195, 210
AfiI CCNNNNNNNGG 2 cut(s) 358, 623
AflIII ACRYGT 4 cut(s) 129, 131, 414, 583
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 2 cut(s) 223, 258
AjuI GAANNNNNNNTTGG 2 cut(s) 477, 509
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 6 cut(s) 313, 319, 472, 544, 656, 735
AluI AGCT 6 cut(s) 313, 319, 472, 544, 656, 735
Alw21I GWGCWC 1 cut(s) 422
Alw26I GTCTC 2 cut(s) 140, 214
Alw44I GTGCAC 1 cut(s) 418
AlwI GGATC 2 cut(s) 446, 459
AoxI GGCC 2 cut(s) 109, 300
ApaLI GTGCAC 1 cut(s) 418
ApeKI GCWGC 2 cut(s) 14, 632
AspLEI GCGC 2 cut(s) 197, 212
AspS9I GGNCC 2 cut(s) 174, 555
AsuC2I CCSGG 2 cut(s) 108, 428
AsuHPI GGTGA 3 cut(s) 34, 130, 274
AvaII GGWCC 2 cut(s) 174, 555
AxyI CCTNAGG 1 cut(s) 642
BaeGI GKGCMC 1 cut(s) 422
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 2 cut(s) 194, 209
BbrPI CACGTG 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 1 cut(s) 422
BbvI GCAGC 1 cut(s) 644
BccI CCATC 2 cut(s) 425, 442
BceAI ACGGC 1 cut(s) 96
BciT130I CCWGG 2 cut(s) 225, 260
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 2 cut(s) 108, 428
BcoDI GTCTC 2 cut(s) 140, 214
BfaI CTAG 1 cut(s) 722
BfoI RGCGCY 2 cut(s) 198, 213
BfuAI ACCTGC 1 cut(s) 599
BisI GCNGC 4 cut(s) 15, 120, 193, 633
BlsI GCNGC 4 cut(s) 16, 121, 194, 634
Bme1390I CCNGG 4 cut(s) 108, 225, 260, 428
Bme18I GGWCC 2 cut(s) 174, 555
BmgT120I GGNCC 2 cut(s) 174, 555
BmiI GGNNCC 5 cut(s) 196, 211, 453, 556, 557
BmrFI CCNGG 4 cut(s) 108, 225, 260, 428
BmsI GCATC 1 cut(s) 578
BoxI GACNNNNGTC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 492
BpmI CTGGAG 1 cut(s) 335
BpuMI CCSGG 2 cut(s) 108, 428
BsaAI YACGTR 1 cut(s) 132
BsaHI GRCGYC 3 cut(s) 117, 195, 210
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 4 cut(s) 106, 170, 190, 205
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 623
Bse21I CCTNAGG 1 cut(s) 642
Bse3DI GCAATG 1 cut(s) 235
BseBI CCWGG 2 cut(s) 225, 260
BseDI CCNNGG 4 cut(s) 106, 170, 190, 205
BseGI GGATG 2 cut(s) 347, 436
BseLI CCNNNNNNNGG 2 cut(s) 358, 623
BseMI GCAATG 1 cut(s) 235
BseRI GAGGAG 4 cut(s) 72, 146, 156, 383
BseSI GKGCMC 1 cut(s) 422
BseX3I CGGCCG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 644
BsgI GTGCAG 1 cut(s) 577
Bsh1236I CGCG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 111, 302
BshNI GGYRCC 2 cut(s) 194, 209
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 422
BsiSI CCGG 2 cut(s) 108, 427
BslFI GGGAC 2 cut(s) 376, 568
BslI CCNNNNNNNGG 2 cut(s) 358, 623
BsmAI GTCTC 2 cut(s) 140, 214
BsmFI GGGAC 2 cut(s) 376, 568
BsmI GAATGC 2 cut(s) 277, 508
BsnI GGCC 2 cut(s) 111, 302
Bso31I GGTCTC 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 422
Bsp143I GATC 3 cut(s) 247, 451, 466
BspACI CCGC 6 cut(s) 8, 120, 142, 177, 190, 192
BspANI GGCC 2 cut(s) 111, 302
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 5 cut(s) 196, 211, 453, 556, 557
BspMI ACCTGC 1 cut(s) 599
BspPI GGATC 2 cut(s) 446, 459
BspT107I GGYRCC 2 cut(s) 194, 209
BspTNI GGTCTC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 235
BssECI CCNNGG 4 cut(s) 106, 170, 190, 205
BssMI GATC 3 cut(s) 247, 451, 466
BssNI GRCGYC 3 cut(s) 117, 195, 210
BssT1I CCWWGG 2 cut(s) 170, 205
Bst2UI CCWGG 2 cut(s) 225, 260
Bst4CI ACNGT 1 cut(s) 598
Bst6I CTCTTC 2 cut(s) 76, 286
BstACI GRCGYC 3 cut(s) 117, 195, 210
BstBAI YACGTR 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 642
BstDSI CCRYGG 1 cut(s) 190
BstF5I GGATG 2 cut(s) 347, 436
BstFNI CGCG 1 cut(s) 192
BstH2I RGCGCY 2 cut(s) 198, 213
BstHHI GCGC 2 cut(s) 197, 212
BstKTI GATC 3 cut(s) 250, 454, 469
BstMAI GTCTC 2 cut(s) 140, 214
BstMBI GATC 3 cut(s) 247, 451, 466
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 2 cut(s) 14, 469
BstNI CCWGG 2 cut(s) 225, 260
BstNSI RCATGY 3 cut(s) 418, 587, 721
BstPAI GACNNNNGTC 1 cut(s) 132
BstSCI CCNGG 4 cut(s) 106, 223, 258, 426
BstSLI GKGCMC 1 cut(s) 422
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 644
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BstZI CGGCCG 1 cut(s) 109
Bsu36I CCTNAGG 1 cut(s) 642
BsuRI GGCC 2 cut(s) 111, 302
BtgI CCRYGG 1 cut(s) 190
BtsCI GGATG 2 cut(s) 347, 436
BtsIMutI CAGTG 1 cut(s) 594
BveI ACCTGC 1 cut(s) 599
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 197, 212
Cfr13I GGNCC 2 cut(s) 174, 555
Cfr42I CCGCGG 1 cut(s) 193
CseI GACGC 1 cut(s) 125
CsiI ACCWGGT 1 cut(s) 258
CviAII CATG 5 cut(s) 284, 394, 415, 584, 718
DdeI CTNAG 1 cut(s) 642
DinI GGCGCC 2 cut(s) 196, 211
DpnI GATC 3 cut(s) 249, 453, 468
DpnII GATC 3 cut(s) 247, 451, 466
DrdI GACNNNNNNGTC 1 cut(s) 370
DseDI GACNNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 1 cut(s) 109
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 76, 286
EarI CTCTTC 2 cut(s) 76, 286
EciI GGCGGA 1 cut(s) 166
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 2 cut(s) 170, 205
Eco31I GGTCTC 1 cut(s) 214
Eco47I GGWCC 2 cut(s) 174, 555
Eco52I CGGCCG 1 cut(s) 109
Eco72I CACGTG 1 cut(s) 132
Eco81I CCTNAGG 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 555
EcoRII CCWGG 2 cut(s) 223, 258
EcoT14I CCWWGG 2 cut(s) 170, 205
EgeI GGCGCC 2 cut(s) 196, 211
EheI GGCGCC 2 cut(s) 196, 211
ErhI CCWWGG 2 cut(s) 170, 205
FaeI CATG 5 cut(s) 287, 397, 418, 587, 721
FaqI GGGAC 2 cut(s) 376, 568
FatI CATG 5 cut(s) 283, 393, 414, 583, 717
FauI CCCGC 1 cut(s) 149
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 4 cut(s) 15, 120, 193, 633
FokI GGATG 2 cut(s) 334, 443
Fsp4HI GCNGC 4 cut(s) 15, 120, 193, 633
FspBI CTAG 1 cut(s) 722
GlaI GCGC 2 cut(s) 196, 211
GluI GCNGC 4 cut(s) 15, 120, 193, 633
GsuI CTGGAG 1 cut(s) 335
HaeII RGCGCY 2 cut(s) 198, 213
HaeIII GGCC 2 cut(s) 111, 302
HapII CCGG 2 cut(s) 108, 427
HgaI GACGC 1 cut(s) 125
HhaI GCGC 2 cut(s) 197, 212
Hin1I GRCGYC 3 cut(s) 117, 195, 210
Hin1II CATG 5 cut(s) 287, 397, 418, 587, 721
Hin6I GCGC 2 cut(s) 195, 210
HinP1I GCGC 2 cut(s) 195, 210
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 4 cut(s) 201, 235, 332, 371
HpaII CCGG 2 cut(s) 108, 427
HphI GGTGA 3 cut(s) 34, 130, 274
Hpy166II GTNNAC 2 cut(s) 331, 420
Hpy188III TCNNGA 2 cut(s) 323, 455
Hpy8I GTNNAC 2 cut(s) 331, 420
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 1 cut(s) 598
HpyCH4IV ACGT 2 cut(s) 131, 489
HpyCH4V TGCA 5 cut(s) 4, 420, 569, 594, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 469
HpyF3I CTNAG 1 cut(s) 642
HpySE526I ACGT 2 cut(s) 131, 489
Hsp92I GRCGYC 3 cut(s) 117, 195, 210
Hsp92II CATG 5 cut(s) 287, 397, 418, 587, 721
HspAI GCGC 2 cut(s) 195, 210
KasI GGCGCC 2 cut(s) 194, 209
KflI GGGWCCC 1 cut(s) 555
Ksp22I TGATCA 1 cut(s) 466
KspI CCGCGG 1 cut(s) 193
Kzo9I GATC 3 cut(s) 247, 451, 466
LmnI GCTCC 2 cut(s) 316, 541
Lsp1109I GCAGC 1 cut(s) 644
LweI GCATC 1 cut(s) 578
MabI ACCWGGT 1 cut(s) 258
MaeI CTAG 1 cut(s) 722
MaeII ACGT 2 cut(s) 131, 489
MaeIII GTNAC 4 cut(s) 113, 404, 586, 689
MalI GATC 3 cut(s) 249, 453, 468
MboI GATC 3 cut(s) 247, 451, 466
MboII GAAGA 7 cut(s) 63, 66, 69, 303, 497, 533, 757
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 422
Mly113I GGCGCC 2 cut(s) 195, 210
MlyI GAGTC 3 cut(s) 210, 326, 380
MmeI TCCRAC 1 cut(s) 114
MseI TTAA 3 cut(s) 53, 309, 549
MslI CAYNNNNRTG 2 cut(s) 282, 413
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 2 cut(s) 108, 427
MspR9I CCNGG 4 cut(s) 108, 225, 260, 428
Mva1269I GAATGC 2 cut(s) 277, 508
MvaI CCWGG 2 cut(s) 225, 260
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 2 cut(s) 14, 469
NarI GGCGCC 2 cut(s) 195, 210
NciI CCSGG 2 cut(s) 108, 428
NdeII GATC 3 cut(s) 247, 451, 466
NlaIII CATG 5 cut(s) 287, 397, 418, 587, 721
NlaIV GGNNCC 5 cut(s) 196, 211, 453, 556, 557
NmuCI GTSAC 2 cut(s) 113, 586
NspI RCATGY 3 cut(s) 418, 587, 721
PciI ACATGT 2 cut(s) 414, 583
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PctI GAATGC 2 cut(s) 277, 508
PfeI GAWTC 1 cut(s) 235
PflMI CCANNNNNTGG 1 cut(s) 358
PkrI GCNGC 4 cut(s) 16, 121, 194, 634
PleI GAGTC 3 cut(s) 209, 326, 379
PluTI GGCGCC 2 cut(s) 198, 213
PmaCI CACGTG 1 cut(s) 132
PmlI CACGTG 1 cut(s) 132
PpsI GAGTC 3 cut(s) 209, 326, 379
Ppu21I YACGTR 1 cut(s) 132
PpuMI RGGWCCY 1 cut(s) 555
PscI ACATGT 2 cut(s) 414, 583
PshAI GACNNNNGTC 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 2 cut(s) 223, 258
PspCI CACGTG 1 cut(s) 132
PspGI CCWGG 2 cut(s) 223, 258
PspN4I GGNNCC 5 cut(s) 196, 211, 453, 556, 557
PspPI GGNCC 2 cut(s) 174, 555
PspPPI RGGWCCY 1 cut(s) 555
PsuI RGATCY 1 cut(s) 451
RseI CAYNNNNRTG 2 cut(s) 282, 413
SacII CCGCGG 1 cut(s) 193
SalI GTCGAC 1 cut(s) 329
SaqAI TTAA 3 cut(s) 53, 309, 549
SatI GCNGC 4 cut(s) 15, 120, 193, 633
Sau3AI GATC 3 cut(s) 247, 451, 466
Sau96I GGNCC 2 cut(s) 174, 555
SchI GAGTC 3 cut(s) 210, 326, 380
ScrFI CCNGG 4 cut(s) 108, 225, 260, 428
SduI GDGCHC 1 cut(s) 422
SexAI ACCWGGT 1 cut(s) 258
SfaNI GCATC 1 cut(s) 578
SfoI GGCGCC 2 cut(s) 196, 211
Sfr303I CCGCGG 1 cut(s) 193
SgrBI CCGCGG 1 cut(s) 193
SinI GGWCC 2 cut(s) 174, 555
SmiMI CAYNNNNRTG 2 cut(s) 282, 413
SsiI CCGC 6 cut(s) 8, 120, 142, 177, 190, 192
SspDI GGCGCC 2 cut(s) 194, 209
SspI AATATT 1 cut(s) 708
SspMI CTAG 1 cut(s) 722
StyD4I CCNGG 4 cut(s) 106, 223, 258, 426
StyI CCWWGG 2 cut(s) 170, 205
TaaI ACNGT 1 cut(s) 598
TaiI ACGT 2 cut(s) 134, 492
TaqI TCGA 2 cut(s) 330, 474
TauI GCSGC 2 cut(s) 122, 195
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 3 cut(s) 53, 309, 549
Tru9I TTAA 3 cut(s) 53, 309, 549
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 2 cut(s) 113, 586
TseI GCWGC 2 cut(s) 14, 632
Tsp45I GTSAC 2 cut(s) 113, 586
TspDTI ATGAA 3 cut(s) 300, 498, 542
TspRI CASTG 1 cut(s) 601
Van91I CCANNNNNTGG 1 cut(s) 358
VneI GTGCAC 1 cut(s) 418
VpaK11BI GGWCC 2 cut(s) 174, 555
XceI RCATGY 3 cut(s) 418, 587, 721
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.