Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004357

Physical Location & Seq

Genomic Coordinates Reverse (-)

3511777 .. 3516305

Gene Structure 4529 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1044400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2199 bp

ATGGCCGGCATTGATGATAACATTGCCATAATTGGAGATTGGGTGCCTCCAAGTCCAAGCCCAAGAGCCTTCTTCTCGTCAATGTTAGTTGACGACATTGGTTCAAGATCAATCTTGGAACCTCACAACAACAATAGAACAGAGGATTTCTTCCTGGGAGCTCGAGAAGGAGATAACAACGGAAAAAATTGGTCACAAGGCAATGTTTCTGGTGAGGATATAACTGAAGTGGGCTCATTTCCGGAGTATAAGTCAAACTCACGCGGAGGGCTTGTGGAAAGGATTGCAGCCAGAGCTGGGTTTAATGCTCCGCGGCTGAATACTGAAAGCATTAGATCTTCTGATCTTTCACATACTTCTGACATTCGGTCTCCTTATCTGACAATACCTCCTGGTCTCAGTCCAACCACACTGCTAGATTCTCCTGTTTTCCTTTCAAATTCATTGGCACAGCCATCTCCAACAACCGGAAAATTTCCATTTGCCTCAAATGGCCATGGCAGGAGTTCCACATTAATGCCAGAGAGCCCTGACAAAAATAATTTCTTTGAGGACATAAATGCTTCCTTCGCTTTCAAGCCTGTTGCGGAATCAGGCTCCTTCTTTCTCGGTCTGACCGGAAAAATGGGTTCGGGTACTTTTCAGCAGCAATCCTTTCCCAGCATGGAGGTTTCAGTCCAGTCCGAAAATTCTTCTCAAAGTATAGAACCAACCAAAGTTCAAAACCAGAATACAAACAATCTTCAGCACCAGGCAGACTTCTCTCACACGTCTACTGAAAAAGACAATGGAGCTTACACTATCTCTGCAGACCCTAGGGCTTTCGATACGGTGGGTGGCAGTACTGAACATTCTCCACCCCTTGATGAGCAACCAGACGAAGAAAGAGGCAGTGGTGATTCCATGGCGGCTGGTGGTGGTGGTGCAGCGTCTGAAGATGGATATAATTGGAGAAAATATGGACAGAAACAAGTAAAAGGTAGTGAGTATCCACGGAGTTATTACAAGTGCACACATCCAAATTGTCAGGTTAAGAAAAAGGTTGAACGATCTCAAGAGGGTCATGTAACAGAGATCATCTACAAAGGGGCCCATAACCACCCTAAACCTCCTCCCAATCGTCGATCAGCTGCCATGGGATCATCCAACCCACTTGTTGACATGCGAACAGACATCCCCGAACAAGGTGGTGCTGATGGTGATTCAATTTGGGCAAATACACAAAAGGGGAATGTTGGAGGTCCCGATTGGAAGCATGACAACCTTGAGGTGACTTCGTCAGCATCCGTGGGCCCTGACTACTGCAACCAATCCTCTATGCAGGCGCAGAATGGTACGCACCACAAATCAGGTGATGTGGTGGACGCATCATCTACCTTTTCTAATGATGAAGAGGAAGATGATCGGGGGACACATGGCAGCGTTTCATTGGCCTATGATGGTGAAGGAGATGAATCTGAATCGAAAAGAAGGAAAATTGAAGCCTATGCAACGGAAATGAGTGGAGCTACCAGAGCCATTCGCGAGCCTAGAGTCGTGGTCCAGACAACCAGTGAAGTAGACATCCTTGATGATGGATATCGTTGGCGCAAGTACGGGCAGAAGGTTGTGAAAGGAAATCCGAATCCAAGGAGTTACTACAAGTGCACAAATGCTGGCTGCACTGTGAGAAAGCATGTGGAGAGAGCATCCCATGACCTCAAGTCCGTGATCACCACCTACGAGGGAAAGCACAATCATGATGTTCCCGCTGCTCGAAACAGTAGCCATGTCAATTCCGGCCCTTCCAGCAACATGCCTGGCCAAGCTTCCACTATCCAAACCCACCCACACAGGCCAGAGCCACAAGTTCACAACGGCATGGGGAGATTCGAAAGGCCATCATCAATGGGTTCATTCAACCTACCTGGAAGGCAGCAGATGGGCCCGTCCCACGCTTTCTCTTTCGGGATGAACCAGCCTGGCTTCGCCAATCTGTCAATGGCCGGGTTAGGTCCTGGCCAACCGAAACTCTCGGTTCTGCCTTCTCACCATCCTTACTTTGCACAACAGCGCCATCAGGTCAATGAAATGGCATTCATGTTACCGAAAGGAGAACCAAAAGTGGAGCCAATGTCAGAACCAGGACTAATCATGTCCAATGGCTCATCAGTATACCAACAGCTTATGAGTAGGCTTCCTCTTGGACCACAGATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007163 GO:0007275 GO:0007389 GO:0008150 GO:0009266 GO:0009409 GO:0009555 GO:0009628 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009889 GO:0009942 GO:0009960 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030010 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

733

Amino Acids

79.51

Weight (kDa)

5.96

Isoelectric Point (pI)

51.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	312 - 368	2.5e-26	WRKY DNA -binding domain
WRKY	PF03106	524 - 581	2.6e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004357)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26440 AT5G56270
fragaria_vesca	FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430
malus_domestica	MD03G1044400.v1.1
prunus_persica	Prupe.6G036300_v2.0.a1 Prupe.6G036300_v2.0.a1 Prupe.6G036300_v2.0.a1 Prupe.6G036300_v2.0.a1
pyrus_communis	pycom03g03430
rosa_chinensis	RchiOBHm_Chr5g0074411
rosa_laevigata	RLG00000036473
rosa_multiflora	Rmu_sc0000573.1_g000009
rosa_roxburghii	Rroxscaffold_1G00007130
rosa_rugosa	Rorug05G0430900 Rorug05G0431000 Rorug05G0431100 Rorug05G0431200 Rorug05G0431300 Rorug05G0431400
rosa_samantha	Rh5AG488600 Rh5BG509400 Rh5CG533200 Rh5DG522100
rosa_wichuraiana	Rw5G045310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	43
AccI	GTMKAC	3 cut(s)	773, 1560, 2154
AccII	CGCG	3 cut(s)	264, 313, 1524
AccIII	TCCGGA	1 cut(s)	241
AciI	CCGC	6 cut(s)	264, 311, 313, 587, 908, 1749
AclWI	GGATC	1 cut(s)	1147
AcoI	YGGCCR	5 cut(s)	3, 493, 1801, 1983, 1999
AcsI	RAATTY	3 cut(s)	439, 473, 688
AcuI	CTGAAG	3 cut(s)	246, 728, 954
AfaI	GTAC	4 cut(s)	637, 844, 1336, 1595
AfiI	CCNNNNNNNGG	3 cut(s)	297, 467, 1184
AflIII	ACRYGT	1 cut(s)	768
AgsI	TTSAA	8 cut(s)	105, 438, 577, 722, 1046, 1206, 1481, 1900
AhdI	GACNNNNNGTC	2 cut(s)	367, 1702
AjiI	CACGTC	1 cut(s)	771
AjnI	CCWGG	8 cut(s)	153, 391, 750, 1798, 1906, 1960, 1996, 2122
AluBI	AGCT	7 cut(s)	161, 296, 794, 1130, 1508, 1808, 2164
AluI	AGCT	7 cut(s)	161, 296, 794, 1130, 1508, 1808, 2164
Alw21I	GWGCWC	3 cut(s)	163, 1013, 1649
Alw26I	GTCTC	2 cut(s)	375, 401
Alw44I	GTGCAC	2 cut(s)	1009, 1645
AlwI	GGATC	1 cut(s)	1147
AlwNI	CAGNNNCTG	1 cut(s)	932
Ama87I	CYCGRG	1 cut(s)	162
Aor13HI	TCCGGA	1 cut(s)	241
ApaI	GGGCCC	3 cut(s)	1093, 1295, 1928
ApaLI	GTGCAC	2 cut(s)	1009, 1645
ApeKI	GCWGC	8 cut(s)	287, 646, 926, 1130, 1419, 1659, 1751, 1915
ApoI	RAATTY	3 cut(s)	439, 473, 688
AseI	ATTAAT	1 cut(s)	515
AspA2I	CCTAGG	1 cut(s)	815
AspLEI	GCGC	3 cut(s)	1327, 1590, 2055
AsuC2I	CCSGG	1 cut(s)	1987
AsuHPI	GGTGA	8 cut(s)	224, 908, 1211, 1282, 1364, 1454, 1705, 2021
AsuII	TTCGAA	1 cut(s)	1872
AvaI	CYCGRG	1 cut(s)	162
AvaII	GGWCC	4 cut(s)	1241, 1540, 1994, 2186
AvrII	CCTAGG	1 cut(s)	815
BaeGI	GKGCMC	5 cut(s)	1013, 1093, 1295, 1649, 1928
BalI	TGGCCA	3 cut(s)	495, 1803, 2001
BanI	GGYRCC	1 cut(s)	43
BanII	GRGCYC	6 cut(s)	163, 236, 530, 1093, 1295, 1928
Bbv12I	GWGCWC	3 cut(s)	163, 1013, 1649
BbvI	GCAGC	8 cut(s)	299, 658, 938, 1117, 1431, 1646, 1738, 1927
BccI	CCATC	9 cut(s)	463, 932, 1190, 1433, 1568, 1888, 1915, 2040, 2064
BceAI	ACGGC	1 cut(s)	1873
BciT130I	CCWGG	8 cut(s)	155, 393, 752, 1800, 1908, 1962, 1998, 2124
BciVI	GTATCC	1 cut(s)	999
BclI	TGATCA	1 cut(s)	1710
BcnI	CCSGG	1 cut(s)	1987
BcoDI	GTCTC	2 cut(s)	375, 401
BfaI	CTAG	3 cut(s)	416, 816, 1530
BfmI	CTRYAG	1 cut(s)	807
BfoI	RGCGCY	1 cut(s)	2056
BfuI	GTATCC	1 cut(s)	999
BglII	AGATCT	1 cut(s)	335
BlnI	CCTAGG	1 cut(s)	815
BmcAI	AGTACT	1 cut(s)	844
Bme1390I	CCNGG	9 cut(s)	155, 393, 752, 1800, 1908, 1962, 1987, 1998, 2124
Bme18I	GGWCC	4 cut(s)	1241, 1540, 1994, 2186
BmeRI	GACNNNNNGTC	2 cut(s)	367, 1702
BmeT110I	CYCGRG	1 cut(s)	162
BmgBI	CACGTC	1 cut(s)	771
BmiI	GGNNCC	9 cut(s)	45, 120, 598, 1090, 1091, 1243, 1293, 1926, 2109
BmrFI	CCNGG	9 cut(s)	155, 393, 752, 1800, 1908, 1962, 1987, 1998, 2124
BmsI	GCATC	3 cut(s)	1292, 1376, 1697
Bpu14I	TTCGAA	1 cut(s)	1872
BpuEI	CTTGAG	3 cut(s)	1038, 1286, 1685
BpuMI	CCSGG	1 cut(s)	1987
BsaBI	GATNNNNATC	1 cut(s)	1578
BsaI	GGTCTC	2 cut(s)	375, 401
BsaJI	CCNNGG	9 cut(s)	154, 311, 496, 815, 903, 992, 1134, 1287, 1628
BsaWI	WCCGGW	3 cut(s)	241, 467, 617
Bsc4I	CCNNNNNNNGG	3 cut(s)	297, 467, 1184
Bse118I	RCCGGY	1 cut(s)	5
Bse1I	ACTGG	2 cut(s)	679, 1551
Bse3DI	GCAATG	2 cut(s)	21, 208
Bse8I	GATNNNNATC	1 cut(s)	1578
BseAI	TCCGGA	1 cut(s)	241
BseBI	CCWGG	8 cut(s)	155, 393, 752, 1800, 1908, 1962, 1998, 2124
BseDI	CCNNGG	9 cut(s)	154, 311, 496, 815, 903, 992, 1134, 1287, 1628
BseGI	GGATG	8 cut(s)	1015, 1142, 1173, 1283, 1563, 1688, 1956, 2032
BseJI	GATNNNNATC	1 cut(s)	1578
BseLI	CCNNNNNNNGG	3 cut(s)	297, 467, 1184
BseMI	GCAATG	2 cut(s)	21, 208
BseMII	CTCAG	1 cut(s)	412
BseNI	ACTGG	2 cut(s)	679, 1551
BseRI	GAGGAG	1 cut(s)	1101
BseSI	GKGCMC	5 cut(s)	1013, 1093, 1295, 1649, 1928
BseXI	GCAGC	8 cut(s)	299, 658, 938, 1117, 1431, 1646, 1738, 1927
BseYI	CCCAGC	2 cut(s)	296, 659
BsgI	GTGCAG	2 cut(s)	945, 1645
Bsh1236I	CGCG	3 cut(s)	264, 313, 1524
BshNI	GGYRCC	1 cut(s)	43
BsiHKAI	GWGCWC	3 cut(s)	163, 1013, 1649
BsiHKCI	CYCGRG	1 cut(s)	162
BsiSI	CCGG	6 cut(s)	6, 242, 468, 618, 1779, 1986
BslFI	GGGAC	3 cut(s)	1227, 1423, 1915
BslI	CCNNNNNNNGG	3 cut(s)	297, 467, 1184
BsmAI	GTCTC	2 cut(s)	375, 401
BsmFI	GGGAC	3 cut(s)	1227, 1423, 1915
BsmI	GAATGC	1 cut(s)	2075
Bso31I	GGTCTC	2 cut(s)	375, 401
BsoBI	CYCGRG	1 cut(s)	162
Bsp119I	TTCGAA	1 cut(s)	1872
Bsp120I	GGGCCC	3 cut(s)	1089, 1291, 1924
Bsp1286I	GDGCHC	8 cut(s)	163, 236, 530, 1013, 1093, 1295, 1649, 1928
Bsp13I	TCCGGA	1 cut(s)	241
Bsp143I	GATC	9 cut(s)	107, 335, 343, 1049, 1074, 1124, 1139, 1402, 1710
Bsp19I	CCATGG	3 cut(s)	496, 903, 1134
Bsp68I	TCGCGA	1 cut(s)	1524
BspACI	CCGC	6 cut(s)	264, 311, 313, 587, 908, 1749
BspCNI	CTCAG	1 cut(s)	411
BspEI	TCCGGA	1 cut(s)	241
BspFNI	CGCG	3 cut(s)	264, 313, 1524
BspHI	TCATGA	1 cut(s)	1738
BspLI	GGNNCC	9 cut(s)	45, 120, 598, 1090, 1091, 1243, 1293, 1926, 2109
BspMAI	CTGCAG	1 cut(s)	811
BspPI	GGATC	1 cut(s)	1147
BspT104I	TTCGAA	1 cut(s)	1872
BspT107I	GGYRCC	1 cut(s)	43
BspTNI	GGTCTC	2 cut(s)	375, 401
BsrDI	GCAATG	2 cut(s)	21, 208
BsrFI	RCCGGY	1 cut(s)	5
BsrI	ACTGG	2 cut(s)	679, 1551
BssAI	RCCGGY	1 cut(s)	5
BssECI	CCNNGG	9 cut(s)	154, 311, 496, 815, 903, 992, 1134, 1287, 1628
BssMI	GATC	9 cut(s)	107, 335, 343, 1049, 1074, 1124, 1139, 1402, 1710
BssNAI	GTATAC	1 cut(s)	2155
BssT1I	CCWWGG	5 cut(s)	496, 815, 903, 1134, 1628
Bst1107I	GTATAC	1 cut(s)	2155
Bst2UI	CCWGG	8 cut(s)	155, 393, 752, 1800, 1908, 1962, 1998, 2124
Bst4CI	ACNGT	3 cut(s)	832, 1666, 1763
Bst6I	CTCTTC	1 cut(s)	1386
BstBI	TTCGAA	1 cut(s)	1872
BstC8I	GCNNGC	4 cut(s)	7, 1323, 1526, 1657
BstDEI	CTNAG	1 cut(s)	398
BstDSI	CCRYGG	6 cut(s)	311, 496, 903, 992, 1134, 1287
BstF5I	GGATG	8 cut(s)	1015, 1142, 1173, 1283, 1563, 1688, 1956, 2032
BstFNI	CGCG	3 cut(s)	264, 313, 1524
BstH2I	RGCGCY	1 cut(s)	2056
BstHHI	GCGC	3 cut(s)	1327, 1590, 2055
BstKTI	GATC	9 cut(s)	110, 338, 346, 1052, 1077, 1127, 1142, 1405, 1713
BstMAI	GTCTC	2 cut(s)	375, 401
BstMBI	GATC	9 cut(s)	107, 335, 343, 1049, 1074, 1124, 1139, 1402, 1710
BstMWI	GCNNNNNNNGC	4 cut(s)	293, 569, 1514, 1788
BstNI	CCWGG	8 cut(s)	155, 393, 752, 1800, 1908, 1962, 1998, 2124
BstNSI	RCATGY	3 cut(s)	1165, 1679, 1798
BstSCI	CCNGG	9 cut(s)	153, 391, 750, 1798, 1906, 1960, 1985, 1996, 2122
BstSFI	CTRYAG	1 cut(s)	807
BstSLI	GKGCMC	5 cut(s)	1013, 1093, 1295, 1649, 1928
BstUI	CGCG	3 cut(s)	264, 313, 1524
BstV1I	GCAGC	8 cut(s)	299, 658, 938, 1117, 1431, 1646, 1738, 1927
BstX2I	RGATCY	1 cut(s)	335
BstYI	RGATCY	1 cut(s)	335
BstZ17I	GTATAC	1 cut(s)	2155
BsuI	GTATCC	1 cut(s)	999
BtgI	CCRYGG	6 cut(s)	311, 496, 903, 992, 1134, 1287
BtrI	CACGTC	1 cut(s)	771
BtsCI	GGATG	8 cut(s)	1015, 1142, 1173, 1283, 1563, 1688, 1956, 2032
BtsI	GCAGTG	2 cut(s)	410, 898
BtsIMutI	CAGTG	4 cut(s)	410, 898, 1558, 1662
BtuMI	TCGCGA	1 cut(s)	1524
Cac8I	GCNNGC	4 cut(s)	7, 1323, 1526, 1657
CaiI	CAGNNNCTG	1 cut(s)	932
CciI	TCATGA	1 cut(s)	1738
CfoI	GCGC	3 cut(s)	1327, 1590, 2055
Cfr10I	RCCGGY	1 cut(s)	5
Cfr42I	CCGCGG	1 cut(s)	314
CseI	GACGC	2 cut(s)	918, 1373
Csp6I	GTAC	4 cut(s)	636, 843, 1335, 1594
CviQI	GTAC	4 cut(s)	636, 843, 1335, 1594
DdeI	CTNAG	1 cut(s)	398
DpnI	GATC	9 cut(s)	109, 337, 345, 1051, 1076, 1126, 1141, 1404, 1712
DpnII	GATC	9 cut(s)	107, 335, 343, 1049, 1074, 1124, 1139, 1402, 1710
DriI	GACNNNNNGTC	2 cut(s)	367, 1702
EaeI	YGGCCR	5 cut(s)	3, 493, 1801, 1983, 1999
Eam1104I	CTCTTC	1 cut(s)	1386
Eam1105I	GACNNNNNGTC	2 cut(s)	367, 1702
EarI	CTCTTC	1 cut(s)	1386
Ecl136II	GAGCTC	1 cut(s)	161
Eco130I	CCWWGG	5 cut(s)	496, 815, 903, 1134, 1628
Eco24I	GRGCYC	6 cut(s)	163, 236, 530, 1093, 1295, 1928
Eco31I	GGTCTC	2 cut(s)	375, 401
Eco32I	GATATC	1 cut(s)	1580
Eco47I	GGWCC	4 cut(s)	1241, 1540, 1994, 2186
Eco53kI	GAGCTC	1 cut(s)	161
Eco57I	CTGAAG	3 cut(s)	246, 728, 954
Eco88I	CYCGRG	1 cut(s)	162
EcoICRI	GAGCTC	1 cut(s)	161
EcoO109I	RGGNCCY	4 cut(s)	1089, 1241, 1292, 1994
EcoRII	CCWGG	8 cut(s)	153, 391, 750, 1798, 1906, 1960, 1996, 2122
EcoRV	GATATC	1 cut(s)	1580
EcoT14I	CCWWGG	5 cut(s)	496, 815, 903, 1134, 1628
EcoT38I	GRGCYC	6 cut(s)	163, 236, 530, 1093, 1295, 1928
ErhI	CCWWGG	5 cut(s)	496, 815, 903, 1134, 1628
FaqI	GGGAC	3 cut(s)	1227, 1423, 1915
FauI	CCCGC	1 cut(s)	1756
FbaI	TGATCA	1 cut(s)	1710
FblI	GTMKAC	3 cut(s)	773, 1560, 2154
FokI	GGATG	8 cut(s)	1002, 1129, 1160, 1270, 1550, 1675, 1963, 2019
FriOI	GRGCYC	6 cut(s)	163, 236, 530, 1093, 1295, 1928
FspBI	CTAG	3 cut(s)	416, 816, 1530
GlaI	GCGC	3 cut(s)	1326, 1589, 2054
GsaI	CCCAGC	2 cut(s)	300, 663
HaeII	RGCGCY	1 cut(s)	2056
HapII	CCGG	6 cut(s)	6, 242, 468, 618, 1779, 1986
HgaI	GACGC	2 cut(s)	918, 1373
HhaI	GCGC	3 cut(s)	1327, 1590, 2055
Hin6I	GCGC	3 cut(s)	1325, 1588, 2053
HinP1I	GCGC	3 cut(s)	1325, 1588, 2053
HincII	GTYRAC	2 cut(s)	91, 1159
HindII	GTYRAC	2 cut(s)	91, 1159
HindIII	AAGCTT	1 cut(s)	1806
HinfI	GANTC	9 cut(s)	419, 590, 899, 1202, 1454, 1460, 1533, 1624, 1869
HpaII	CCGG	6 cut(s)	6, 242, 468, 618, 1779, 1986
HphI	GGTGA	8 cut(s)	224, 908, 1211, 1282, 1364, 1454, 1705, 2021
Hpy166II	GTNNAC	9 cut(s)	91, 774, 1011, 1159, 1363, 1561, 1647, 1852, 2155
Hpy188I	TCNGA	9 cut(s)	343, 361, 381, 615, 685, 934, 1459, 1623, 2119
Hpy188III	TCNNGA	9 cut(s)	105, 164, 242, 1055, 1244, 1523, 1543, 1739, 1948
Hpy8I	GTNNAC	9 cut(s)	91, 774, 1011, 1159, 1363, 1561, 1647, 1852, 2155
Hpy99I	CGWCG	1 cut(s)	1125
HpyCH4III	ACNGT	3 cut(s)	832, 1666, 1763
HpyCH4IV	ACGT	1 cut(s)	770
HpyF10VI	GCNNNNNNNGC	4 cut(s)	293, 569, 1514, 1788
HpyF3I	CTNAG	1 cut(s)	398
HpySE526I	ACGT	1 cut(s)	770
HspAI	GCGC	3 cut(s)	1325, 1588, 2053
Kpn2I	TCCGGA	1 cut(s)	241
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Ksp22I	TGATCA	1 cut(s)	1710
KspI	CCGCGG	1 cut(s)	314
Kzo9I	GATC	9 cut(s)	107, 335, 343, 1049, 1074, 1124, 1139, 1402, 1710
LmnI	GCTCC	6 cut(s)	158, 313, 602, 791, 1505, 2107
Lsp1109I	GCAGC	8 cut(s)	299, 658, 938, 1117, 1431, 1646, 1738, 1927
LweI	GCATC	3 cut(s)	1292, 1376, 1697
MaeI	CTAG	3 cut(s)	416, 816, 1530
MaeII	ACGT	1 cut(s)	770
MaeIII	GTNAC	5 cut(s)	192, 1066, 1270, 1634, 2082
MalI	GATC	9 cut(s)	109, 337, 345, 1051, 1076, 1126, 1141, 1404, 1712
MboI	GATC	9 cut(s)	107, 335, 343, 1049, 1074, 1124, 1139, 1402, 1710
MboII	GAAGA	9 cut(s)	64, 142, 330, 684, 734, 893, 947, 1403, 1409
MflI	RGATCY	1 cut(s)	335
MhlI	GDGCHC	8 cut(s)	163, 236, 530, 1013, 1093, 1295, 1649, 1928
MlsI	TGGCCA	3 cut(s)	495, 1803, 2001
MluNI	TGGCCA	3 cut(s)	495, 1803, 2001
MlyI	GAGTC	1 cut(s)	1542
MmeI	TCCRAC	4 cut(s)	428, 485, 1170, 1216
Mox20I	TGGCCA	3 cut(s)	495, 1803, 2001
MroI	TCCGGA	1 cut(s)	241
MroNI	GCCGGC	1 cut(s)	5
MscI	TGGCCA	3 cut(s)	495, 1803, 2001
MseI	TTAA	3 cut(s)	303, 515, 1032
MslI	CAYNNNNRTG	2 cut(s)	515, 1737
Msp20I	TGGCCA	3 cut(s)	495, 1803, 2001
MspA1I	CMGCKG	3 cut(s)	313, 1130, 1751
MspI	CCGG	6 cut(s)	6, 242, 468, 618, 1779, 1986
MspR9I	CCNGG	9 cut(s)	155, 393, 752, 1800, 1908, 1962, 1987, 1998, 2124
Mva1269I	GAATGC	1 cut(s)	2075
MvaI	CCWGG	8 cut(s)	155, 393, 752, 1800, 1908, 1962, 1998, 2124
MvnI	CGCG	3 cut(s)	264, 313, 1524
MwoI	GCNNNNNNNGC	4 cut(s)	293, 569, 1514, 1788
NaeI	GCCGGC	1 cut(s)	7
NciI	CCSGG	1 cut(s)	1987
NcoI	CCATGG	3 cut(s)	496, 903, 1134
NdeII	GATC	9 cut(s)	107, 335, 343, 1049, 1074, 1124, 1139, 1402, 1710
NgoMIV	GCCGGC	1 cut(s)	5
NlaIV	GGNNCC	9 cut(s)	45, 120, 598, 1090, 1091, 1243, 1293, 1926, 2109
NmuCI	GTSAC	2 cut(s)	192, 1270
NruI	TCGCGA	1 cut(s)	1524
NspI	RCATGY	3 cut(s)	1165, 1679, 1798
NspV	TTCGAA	1 cut(s)	1872
PaeR7I	CTCGAG	1 cut(s)	162
PagI	TCATGA	1 cut(s)	1738
PctI	GAATGC	1 cut(s)	2075
PdiI	GCCGGC	1 cut(s)	7
PfeI	GAWTC	8 cut(s)	419, 590, 899, 1202, 1454, 1460, 1624, 1869
PflFI	GACNNNGTC	1 cut(s)	1276
PleI	GAGTC	1 cut(s)	1541
PpsI	GAGTC	1 cut(s)	1541
PpuMI	RGGWCCY	2 cut(s)	1241, 1994
PshBI	ATTAAT	1 cut(s)	515
Psp124BI	GAGCTC	1 cut(s)	163
Psp5II	RGGWCCY	2 cut(s)	1241, 1994
Psp6I	CCWGG	8 cut(s)	153, 391, 750, 1798, 1906, 1960, 1996, 2122
PspFI	CCCAGC	2 cut(s)	296, 659
PspGI	CCWGG	8 cut(s)	153, 391, 750, 1798, 1906, 1960, 1996, 2122
PspN4I	GGNNCC	9 cut(s)	45, 120, 598, 1090, 1091, 1243, 1293, 1926, 2109
PspOMI	GGGCCC	3 cut(s)	1089, 1291, 1924
PspPPI	RGGWCCY	2 cut(s)	1241, 1994
PstI	CTGCAG	1 cut(s)	811
PstNI	CAGNNNCTG	1 cut(s)	932
PsuI	RGATCY	1 cut(s)	335
PsyI	GACNNNGTC	1 cut(s)	1276
PvuII	CAGCTG	1 cut(s)	1130
RruI	TCGCGA	1 cut(s)	1524
RsaI	GTAC	4 cut(s)	637, 844, 1336, 1595
RsaNI	GTAC	4 cut(s)	636, 843, 1335, 1594
RseI	CAYNNNNRTG	2 cut(s)	515, 1737
SacI	GAGCTC	1 cut(s)	163
SacII	CCGCGG	1 cut(s)	314
SaqAI	TTAA	3 cut(s)	303, 515, 1032
Sau3AI	GATC	9 cut(s)	107, 335, 343, 1049, 1074, 1124, 1139, 1402, 1710
ScaI	AGTACT	1 cut(s)	844
SchI	GAGTC	1 cut(s)	1542
ScrFI	CCNGG	9 cut(s)	155, 393, 752, 1800, 1908, 1962, 1987, 1998, 2124
SduI	GDGCHC	8 cut(s)	163, 236, 530, 1013, 1093, 1295, 1649, 1928
SfaNI	GCATC	3 cut(s)	1292, 1376, 1697
SfcI	CTRYAG	1 cut(s)	807
Sfr274I	CTCGAG	1 cut(s)	162
Sfr303I	CCGCGG	1 cut(s)	314
SfuI	TTCGAA	1 cut(s)	1872
SgrBI	CCGCGG	1 cut(s)	314
SinI	GGWCC	4 cut(s)	1241, 1540, 1994, 2186
SlaI	CTCGAG	1 cut(s)	162
SmiMI	CAYNNNNRTG	2 cut(s)	515, 1737
SmlI	CTYRAG	4 cut(s)	162, 1053, 1265, 1700
SmoI	CTYRAG	4 cut(s)	162, 1053, 1265, 1700
SsiI	CCGC	6 cut(s)	264, 311, 313, 587, 908, 1749
SspMI	CTAG	3 cut(s)	416, 816, 1530
SstI	GAGCTC	1 cut(s)	163
StyD4I	CCNGG	9 cut(s)	153, 391, 750, 1798, 1906, 1960, 1985, 1996, 2122
StyI	CCWWGG	5 cut(s)	496, 815, 903, 1134, 1628
TaaI	ACNGT	3 cut(s)	832, 1666, 1763
TaiI	ACGT	1 cut(s)	773
TaqI	TCGA	6 cut(s)	163, 825, 1123, 1463, 1756, 1872
TaqII	GACCGA	2 cut(s)	357, 599
TatI	WGTACW	1 cut(s)	842
TauI	GCSGC	2 cut(s)	316, 911
TfiI	GAWTC	8 cut(s)	419, 590, 899, 1202, 1454, 1460, 1624, 1869
Tru1I	TTAA	3 cut(s)	303, 515, 1032
Tru9I	TTAA	3 cut(s)	303, 515, 1032
TscAI	CASTG	4 cut(s)	417, 898, 1558, 1669
TseFI	GTSAC	2 cut(s)	192, 1270
TseI	GCWGC	8 cut(s)	287, 646, 926, 1130, 1419, 1659, 1751, 1915
Tsp45I	GTSAC	2 cut(s)	192, 1270
TspDTI	ATGAA	8 cut(s)	432, 1404, 1416, 1467, 1884, 1967, 2068, 2082
TspGWI	ACGGA	5 cut(s)	195, 1009, 1276, 1508, 1696
TspRI	CASTG	4 cut(s)	417, 898, 1558, 1669
Tth111I	GACNNNGTC	1 cut(s)	1276
VneI	GTGCAC	2 cut(s)	1009, 1645
VpaK11BI	GGWCC	4 cut(s)	1241, 1540, 1994, 2186
VspI	ATTAAT	1 cut(s)	515
XapI	RAATTY	3 cut(s)	439, 473, 688
XceI	RCATGY	3 cut(s)	1165, 1679, 1798
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1978
XhoI	CTCGAG	1 cut(s)	162
XmaJI	CCTAGG	1 cut(s)	815
XmiI	GTMKAC	3 cut(s)	773, 1560, 2154
XspI	CTAG	3 cut(s)	416, 816, 1530
ZrmI	AGTACT	1 cut(s)	844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.