Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0004357

Physical Location & Seq

Genomic Coordinates Forward (+)

81017369 .. 81020698

Gene Structure 3330 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG522100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2199 bp

ATGGCTGGCATGGATGATAACGTTGCTATAATTGGGGACTGGGTGCCTCCGAGTCCAAGCCCAAGGGACTTCTTCTCTTCAATGTTAGTTGATGGCATTGGCTCAAGGTCATTCTTGGAATCTCCTAGCAGTAATAAAGCTGAGGAGTTCTGGTCTCGAGAAGGAAATACCAACGGAAAGAACTTCTCACAAGAAAATGGTACCGGTGATGAAATAACTGAAATGGGTTCATTTTCAGAGTACAAATCTAACTCACGTGGGGGACTTGTGGATCGGATCGCAGCCAGAGCTGGTTTTAATGCTCCGCGGTTGAATACAGAAAGCATTAGATCTTCTGACCTTTCACTTAATTCTGATGTTCGGTCTCCTTATCTGACAATACCCCCTGGTCTCAGTCCAACCACACTGCTAGACTCTCCTGTCTTCCTTTCAAATTCATTGGCACAGCCTTCTCCAACAACTGGAAAATTTCCATTTGTCTCAAATGGTCACGCCAGGAATTCCACATTGATGACAGAGGCTGCTGATAAAGCTAATTTCTTTGAGGACATGAATACTTCATTTGCTTTCAAGCCTCTTGGCGAATCAGGCTCTTTCTTTCTTGGTCCAACAAGCAAGCAATCCTTTCCCAGCATTGAGGTGTCAGTCCACTCAGAAAACTCTTCTCAAAGTATGGAACCCACCAAAGTTCAAAATCAGAACGCGAACAATTTTCAGCTCCAGCCAGATTTTTCTCGCACATCTACTGAAAAAGATAATGGAGCAAATACAGTCTCTGCAGATCCGAGGGCTTTTGATACTGTTGGTGGCAGTACTGAACATTCTCCACCCCTTGATGAGCAACCAGAGGAAGAAGGAGATCAAAGAGGTGGTGGTGATTCCATGGCTGCTGCTGGTGGAGGTACACCATCTGAAGATGGATATAATTGGAGAAAATATGGACAAAAACAAGTAAAAGGTAGTGAGTACCCACGAAGTTATTACAAGTGCACGCATCCAAATTGTCAGGTTAAGAAAAAGGTTGAACGATCTCATGAGGGTCATATAACAGAGATCATCTACAAGGGGGCCCATAACCACCCTAAACCCCCTCCCAATCGTAGATCAGGGGCCATTGGATCATCTAACCCACTCATTGACATGCGATCGGATGTCCTTGAACAAGGCGGACCACAGACAGGTGCTGATGGTGATCTGGTTTGGGCAAGTGCACAAAAGGCAACTGGTGGAGCTCCTGATTGGAAGCATGACAACCTTGAGGTGACTTCATCAGCATCAGCGGGCCCTGACTACGGCCAGCAATCCACTTCTATGCAGGCTCAGACTGGTGCACACCTTGAATCAGGTGATGTAGTAGACGCATCATCTACCTTTTCCAATGATGAGGATGAAGATGATCGGGGAACGCATGGTAGTGTTTCATTAGCCTATGATGGTGAAGGAGATGAGTCAGAGTCGAAAAGAAGGAAAATTGAAGCCTATGCAACAGAAATGAGTGGAGCTACAAGAGCTATTCGCGAGCCTAGAGTTGTAGTCCAGACTACCAGTGAAGTAGATATACTTGATGATGGATATCGTTGGCGCAAGTATGGGCAGAAAGTAGTGAAAGGGAATCCAAATCCAAGGAGTTACTATAAATGCACCAATGCTGGTTGTACAGTGAGGAAGCATGTGGAGAGAGCATCCCATGACCTCAAGTCAGTCATCACCACATATGAAGGAAAGCACAATCATGATGTTCCCGCTGCTCGCAATAGTAGCCATGTCAATTCTGGCCCTTCTGGCAACATGTCAGGTCAAGCATCTGGCTCTGCTGCACAAACTCATCCTCATCGGCCAGAGCCATCACAAGTTCACAGTAACATGGCAAGATTTGAAAGGCCCATGTCAATGGGTTCATTCAGCTTAGCTGGAAGGCAGCAGCTGGGTCCTCCCCAGGGCTTCTCTTTCGGAATGAACCAGCCTGGCCTGGCAAATCTGGCAATGGCTGGGTTCAGTCCTGGCCAACACAAAGTGCCTGTTCTCCCTGTTCATCATCCATACTTTGCCCAACAGCGTCAGGTCAGTGAAATGGGTTTCATGTTACCAAAAGGAGAACCAAAAGTAGAGCCAATGTCAGAACCTGGCCTAAACATGAACAATGCTTCATCGGTATACCAACAACTTATGAGTAGGCTTCCCCTTGGACCACAGATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000578 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007163 GO:0007275 GO:0007389 GO:0008150 GO:0009266 GO:0009409 GO:0009555 GO:0009628 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009846 GO:0009856 GO:0009880 GO:0009888 GO:0009889 GO:0009942 GO:0009960 GO:0009987 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030010 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0044706 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

732

Amino Acids

79.1

Weight (kDa)

5.74

Isoelectric Point (pI)

51.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	305 - 361	3.4e-26	WRKY DNA -binding domain
WRKY	PF03106	522 - 579	2.6e-25	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004357)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26440 AT5G56270
fragaria_vesca	FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430 FvH4_3g41430
malus_domestica	MD03G1044400.v1.1
prunus_persica	Prupe.6G036300_v2.0.a1 Prupe.6G036300_v2.0.a1 Prupe.6G036300_v2.0.a1 Prupe.6G036300_v2.0.a1
pyrus_communis	pycom03g03430
rosa_chinensis	RchiOBHm_Chr5g0074411
rosa_laevigata	RLG00000036473
rosa_multiflora	Rmu_sc0000573.1_g000009
rosa_roxburghii	Rroxscaffold_1G00007130
rosa_rugosa	Rorug05G0430900 Rorug05G0431000 Rorug05G0431100 Rorug05G0431200 Rorug05G0431300 Rorug05G0431400
rosa_samantha	Rh5AG488600 Rh5BG509400 Rh5CG533200 Rh5DG522100
rosa_wichuraiana	Rw5G045310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	419
Acc65I	GGTACC	1 cut(s)	200
AccB1I	GGYRCC	2 cut(s)	43, 200
AccB7I	CCANNNNNTGG	1 cut(s)	461
AccI	GTMKAC	2 cut(s)	1356, 2154
AccII	CGCG	3 cut(s)	307, 704, 1518
AciI	CCGC	5 cut(s)	305, 307, 1167, 1280, 1743
AclI	AACGTT	1 cut(s)	21
AclWI	GGATC	4 cut(s)	279, 284, 776, 1126
AcoI	YGGCCR	3 cut(s)	1294, 1835, 2002
AcsI	RAATTY	3 cut(s)	433, 467, 499
AcuI	CTGAAG	1 cut(s)	933
AcvI	CACGTG	1 cut(s)	257
AdeI	CACNNNGTG	1 cut(s)	2014
AfaI	GTAC	6 cut(s)	202, 242, 814, 904, 968, 1657
AfiI	CCNNNNNNNGG	4 cut(s)	461, 1178, 1292, 1937
AflIII	ACRYGT	1 cut(s)	1788
AgeI	ACCGGT	1 cut(s)	203
AhdI	GACNNNNNGTC	1 cut(s)	1696
AjnI	CCWGG	7 cut(s)	385, 494, 1935, 1963, 1968, 1999, 2122
Alw21I	GWGCWC	4 cut(s)	992, 1213, 1234, 1333
Alw26I	GTCTC	5 cut(s)	159, 369, 395, 484, 778
Alw44I	GTGCAC	3 cut(s)	988, 1209, 1329
AlwI	GGATC	4 cut(s)	279, 284, 776, 1126
AlwNI	CAGNNNCTG	5 cut(s)	521, 776, 1184, 1924, 2123
Ama87I	CYCGRG	1 cut(s)	156
ApaI	GGGCCC	2 cut(s)	1072, 1286
ApaLI	GTGCAC	3 cut(s)	988, 1209, 1329
ApeKI	GCWGC	8 cut(s)	281, 521, 887, 890, 1745, 1814, 1918, 1921
ApoI	RAATTY	3 cut(s)	433, 467, 499
AsiGI	ACCGGT	1 cut(s)	203
Asp718I	GGTACC	1 cut(s)	200
AspLEI	GCGC	1 cut(s)	1584
AsuHPI	GGTGA	7 cut(s)	218, 887, 1202, 1273, 1358, 1448, 1699
AvaI	CYCGRG	1 cut(s)	156
AvaII	GGWCC	4 cut(s)	605, 1169, 1928, 2186
BaeGI	GKGCMC	5 cut(s)	992, 1072, 1213, 1286, 1333
BalI	TGGCCA	1 cut(s)	2004
BanI	GGYRCC	2 cut(s)	43, 200
BanII	GRGCYC	3 cut(s)	1072, 1234, 1286
BarI	GAAGNNNNNNTAC	2 cut(s)	1542, 1574
BbrPI	CACGTG	1 cut(s)	257
BbsI	GAAGAC	1 cut(s)	415
Bbv12I	GWGCWC	4 cut(s)	992, 1213, 1234, 1333
BbvCI	CCTCAGC	1 cut(s)	141
BbvI	GCAGC	8 cut(s)	293, 508, 874, 877, 1732, 1801, 1930, 1933
BccI	CCATC	7 cut(s)	86, 911, 916, 1181, 1427, 1562, 1852
BceAI	ACGGC	1 cut(s)	1309
BciT130I	CCWGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
BcoDI	GTCTC	5 cut(s)	159, 369, 395, 484, 778
BfaI	CTAG	3 cut(s)	126, 410, 1524
BfmI	CTRYAG	1 cut(s)	777
BglI	GCCNNNNNGGC	1 cut(s)	1782
BglII	AGATCT	1 cut(s)	329
BisI	GCNGC	8 cut(s)	282, 522, 888, 891, 1746, 1815, 1919, 1922
BlpI	GCTNAGC	1 cut(s)	1906
BlsI	GCNGC	8 cut(s)	283, 523, 889, 892, 1747, 1816, 1920, 1923
BmcAI	AGTACT	1 cut(s)	814
Bme1390I	CCNGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
Bme18I	GGWCC	4 cut(s)	605, 1169, 1928, 2186
BmeRI	GACNNNNNGTC	1 cut(s)	1696
BmeT110I	CYCGRG	1 cut(s)	156
BmiI	GGNNCC	8 cut(s)	45, 202, 678, 1069, 1070, 1111, 1284, 1929
BmrFI	CCNGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
BmrI	ACTGGG	1 cut(s)	49
BmsI	GCATC	5 cut(s)	1003, 1283, 1370, 1691, 1811
BmuI	ACTGGG	1 cut(s)	49
BpiI	GAAGAC	1 cut(s)	415
BpmI	CTGGAG	1 cut(s)	704
Bpu10I	CCTNAGC	1 cut(s)	141
Bpu1102I	GCTNAGC	1 cut(s)	1906
BpuEI	CTTGAG	3 cut(s)	88, 1277, 1679
BsaAI	YACGTR	1 cut(s)	257
BsaBI	GATNNNNATC	2 cut(s)	1191, 1572
BsaI	GGTCTC	3 cut(s)	159, 369, 395
BsaJI	CCNNGG	9 cut(s)	62, 305, 385, 785, 882, 1622, 1935, 1936, 2182
BsaWI	WCCGGW	1 cut(s)	203
BsaXI	ACNNNNNCTCC	2 cut(s)	137, 167
Bsc4I	CCNNNNNNNGG	4 cut(s)	461, 1178, 1292, 1937
Bse118I	RCCGGY	1 cut(s)	203
Bse1I	ACTGG	5 cut(s)	44, 466, 1228, 1330, 1545
Bse3DI	GCAATG	1 cut(s)	1989
Bse8I	GATNNNNATC	2 cut(s)	1191, 1572
BseBI	CCWGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
BseDI	CCNNGG	9 cut(s)	62, 305, 385, 785, 882, 1622, 1935, 1936, 2182
BseGI	GGATG	7 cut(s)	19, 994, 1156, 1393, 1682, 1825, 2035
BseJI	GATNNNNATC	2 cut(s)	1191, 1572
BseLI	CCNNNNNNNGG	4 cut(s)	461, 1178, 1292, 1937
BseMI	GCAATG	1 cut(s)	1989
BseMII	CTCAG	4 cut(s)	132, 406, 666, 1334
BseNI	ACTGG	5 cut(s)	44, 466, 1228, 1330, 1545
BseRI	GAGGAG	1 cut(s)	158
BseSI	GKGCMC	5 cut(s)	992, 1072, 1213, 1286, 1333
BseXI	GCAGC	8 cut(s)	293, 508, 874, 877, 1732, 1801, 1930, 1933
BseYI	CCCAGC	3 cut(s)	629, 1924, 1988
BsgI	GTGCAG	1 cut(s)	1800
Bsh1236I	CGCG	3 cut(s)	307, 704, 1518
Bsh1285I	CGRYCG	1 cut(s)	1148
BshNI	GGYRCC	2 cut(s)	43, 200
BshTI	ACCGGT	1 cut(s)	203
BsiEI	CGRYCG	1 cut(s)	1148
BsiHKAI	GWGCWC	4 cut(s)	992, 1213, 1234, 1333
BsiHKCI	CYCGRG	1 cut(s)	156
BsiSI	CCGG	1 cut(s)	204
BslFI	GGGAC	3 cut(s)	50, 80, 276
BslI	CCNNNNNNNGG	4 cut(s)	461, 1178, 1292, 1937
BsmAI	GTCTC	5 cut(s)	159, 369, 395, 484, 778
BsmFI	GGGAC	3 cut(s)	50, 80, 276
Bso31I	GGTCTC	3 cut(s)	159, 369, 395
BsoBI	CYCGRG	1 cut(s)	156
Bsp120I	GGGCCC	2 cut(s)	1068, 1282
Bsp1286I	GDGCHC	6 cut(s)	992, 1072, 1213, 1234, 1286, 1333
Bsp1407I	TGTACA	1 cut(s)	1655
Bsp1720I	GCTNAGC	1 cut(s)	1906
Bsp19I	CCATGG	1 cut(s)	882
Bsp68I	TCGCGA	1 cut(s)	1518
BspACI	CCGC	5 cut(s)	305, 307, 1167, 1280, 1743
BspCNI	CTCAG	4 cut(s)	133, 405, 665, 1333
BspFNI	CGCG	3 cut(s)	307, 704, 1518
BspHI	TCATGA	2 cut(s)	1033, 1732
BspLI	GGNNCC	8 cut(s)	45, 202, 678, 1069, 1070, 1111, 1284, 1929
BspMAI	CTGCAG	1 cut(s)	781
BspPI	GGATC	4 cut(s)	279, 284, 776, 1126
BspT107I	GGYRCC	2 cut(s)	43, 200
BspTNI	GGTCTC	3 cut(s)	159, 369, 395
BsrDI	GCAATG	1 cut(s)	1989
BsrFI	RCCGGY	1 cut(s)	203
BsrGI	TGTACA	1 cut(s)	1655
BsrI	ACTGG	5 cut(s)	44, 466, 1228, 1330, 1545
BssAI	RCCGGY	1 cut(s)	203
BssECI	CCNNGG	9 cut(s)	62, 305, 385, 785, 882, 1622, 1935, 1936, 2182
BssNAI	GTATAC	1 cut(s)	2155
BssT1I	CCWWGG	4 cut(s)	62, 882, 1622, 2182
Bst1107I	GTATAC	1 cut(s)	2155
Bst2UI	CCWGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
Bst4CI	ACNGT	4 cut(s)	772, 802, 1660, 1859
Bst6I	CTCTTC	2 cut(s)	82, 667
BstAUI	TGTACA	1 cut(s)	1655
BstBAI	YACGTR	1 cut(s)	257
BstC8I	GCNNGC	8 cut(s)	7, 617, 992, 1282, 1298, 1317, 1520, 1750
BstDEI	CTNAG	5 cut(s)	141, 392, 652, 1320, 1906
BstDSI	CCRYGG	2 cut(s)	305, 882
BstF5I	GGATG	7 cut(s)	19, 994, 1156, 1393, 1682, 1825, 2035
BstFNI	CGCG	3 cut(s)	307, 704, 1518
BstHHI	GCGC	1 cut(s)	1584
BstMAI	GTCTC	5 cut(s)	159, 369, 395, 484, 778
BstMCI	CGRYCG	1 cut(s)	1148
BstMWI	GCNNNNNNNGC	8 cut(s)	287, 530, 588, 1217, 1508, 1758, 1782, 1979
BstNI	CCWGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
BstNSI	RCATGY	3 cut(s)	1144, 1673, 1792
BstSCI	CCNGG	7 cut(s)	385, 494, 1935, 1963, 1968, 1999, 2122
BstSFI	CTRYAG	1 cut(s)	777
BstSLI	GKGCMC	5 cut(s)	992, 1072, 1213, 1286, 1333
BstUI	CGCG	3 cut(s)	307, 704, 1518
BstV1I	GCAGC	8 cut(s)	293, 508, 874, 877, 1732, 1801, 1930, 1933
BstV2I	GAAGAC	1 cut(s)	415
BstX2I	RGATCY	2 cut(s)	329, 781
BstXI	CCANNNNNNTGG	1 cut(s)	1891
BstYI	RGATCY	2 cut(s)	329, 781
BstZ17I	GTATAC	1 cut(s)	2155
BtgI	CCRYGG	2 cut(s)	305, 882
BtsCI	GGATG	7 cut(s)	19, 994, 1156, 1393, 1682, 1825, 2035
BtsI	GCAGTG	1 cut(s)	404
BtsIMutI	CAGTG	4 cut(s)	404, 1552, 1665, 2071
BtuMI	TCGCGA	1 cut(s)	1518
Cac8I	GCNNGC	8 cut(s)	7, 617, 992, 1282, 1298, 1317, 1520, 1750
CaiI	CAGNNNCTG	5 cut(s)	521, 776, 1184, 1924, 2123
CciI	TCATGA	2 cut(s)	1033, 1732
CfoI	GCGC	1 cut(s)	1584
Cfr10I	RCCGGY	1 cut(s)	203
Cfr42I	CCGCGG	1 cut(s)	308
CseI	GACGC	2 cut(s)	1367, 2045
Csp6I	GTAC	6 cut(s)	201, 241, 813, 903, 967, 1656
CspAI	ACCGGT	1 cut(s)	203
CviQI	GTAC	6 cut(s)	201, 241, 813, 903, 967, 1656
DdeI	CTNAG	5 cut(s)	141, 392, 652, 1320, 1906
DraIII	CACNNNGTG	1 cut(s)	2014
DrdI	GACNNNNNNGTC	1 cut(s)	419
DriI	GACNNNNNGTC	1 cut(s)	1696
DseDI	GACNNNNNNGTC	1 cut(s)	419
EaeI	YGGCCR	3 cut(s)	1294, 1835, 2002
Eam1104I	CTCTTC	2 cut(s)	82, 667
Eam1105I	GACNNNNNGTC	1 cut(s)	1696
EarI	CTCTTC	2 cut(s)	82, 667
EciI	GGCGGA	1 cut(s)	1182
Ecl136II	GAGCTC	1 cut(s)	1232
Eco130I	CCWWGG	4 cut(s)	62, 882, 1622, 2182
Eco24I	GRGCYC	3 cut(s)	1072, 1234, 1286
Eco31I	GGTCTC	3 cut(s)	159, 369, 395
Eco32I	GATATC	1 cut(s)	1574
Eco47I	GGWCC	4 cut(s)	605, 1169, 1928, 2186
Eco53kI	GAGCTC	1 cut(s)	1232
Eco57I	CTGAAG	1 cut(s)	933
Eco72I	CACGTG	1 cut(s)	257
Eco88I	CYCGRG	1 cut(s)	156
EcoICRI	GAGCTC	1 cut(s)	1232
EcoO109I	RGGNCCY	3 cut(s)	1068, 1283, 1928
EcoRI	GAATTC	1 cut(s)	499
EcoRII	CCWGG	7 cut(s)	385, 494, 1935, 1963, 1968, 1999, 2122
EcoRV	GATATC	1 cut(s)	1574
EcoT14I	CCWWGG	4 cut(s)	62, 882, 1622, 2182
EcoT38I	GRGCYC	3 cut(s)	1072, 1234, 1286
ErhI	CCWWGG	4 cut(s)	62, 882, 1622, 2182
FalI	AAGNNNNNCTT	2 cut(s)	608, 640
FaqI	GGGAC	3 cut(s)	50, 80, 276
FauI	CCCGC	2 cut(s)	1273, 1750
FauNDI	CATATG	1 cut(s)	1714
FblI	GTMKAC	2 cut(s)	1356, 2154
Fnu4HI	GCNGC	8 cut(s)	282, 522, 888, 891, 1746, 1815, 1919, 1922
FokI	GGATG	7 cut(s)	26, 981, 1163, 1400, 1669, 1812, 2022
FriOI	GRGCYC	3 cut(s)	1072, 1234, 1286
Fsp4HI	GCNGC	8 cut(s)	282, 522, 888, 891, 1746, 1815, 1919, 1922
FspBI	CTAG	3 cut(s)	126, 410, 1524
GlaI	GCGC	1 cut(s)	1583
GluI	GCNGC	8 cut(s)	282, 522, 888, 891, 1746, 1815, 1919, 1922
GsaI	CCCAGC	3 cut(s)	633, 1928, 1992
GsuI	CTGGAG	1 cut(s)	704
HapII	CCGG	1 cut(s)	204
HgaI	GACGC	2 cut(s)	1367, 2045
HhaI	GCGC	1 cut(s)	1584
Hin6I	GCGC	1 cut(s)	1582
HinP1I	GCGC	1 cut(s)	1582
HinfI	GANTC	9 cut(s)	52, 119, 413, 584, 878, 1340, 1448, 1454, 1612
HpaII	CCGG	1 cut(s)	204
HphI	GGTGA	7 cut(s)	218, 887, 1202, 1273, 1358, 1448, 1699
Hpy166II	GTNNAC	8 cut(s)	649, 905, 990, 1211, 1331, 1357, 1855, 2155
Hpy188III	TCNNGA	7 cut(s)	156, 158, 1034, 1235, 1517, 1537, 1733
Hpy8I	GTNNAC	8 cut(s)	649, 905, 990, 1211, 1331, 1357, 1855, 2155
HpyAV	CCTTC	8 cut(s)	155, 459, 848, 1433, 1458, 1712, 1788, 1908
HpyCH4III	ACNGT	4 cut(s)	772, 802, 1660, 1859
HpyCH4IV	ACGT	2 cut(s)	21, 256
HpyCH4V	TGCA	8 cut(s)	779, 990, 1211, 1315, 1331, 1484, 1641, 1817
HpyF10VI	GCNNNNNNNGC	8 cut(s)	287, 530, 588, 1217, 1508, 1758, 1782, 1979
HpyF3I	CTNAG	5 cut(s)	141, 392, 652, 1320, 1906
HpySE526I	ACGT	2 cut(s)	21, 256
HspAI	GCGC	1 cut(s)	1582
KpnI	GGTACC	1 cut(s)	204
KspI	CCGCGG	1 cut(s)	308
LmnI	GCTCC	6 cut(s)	307, 723, 761, 1229, 1237, 1499
Lsp1109I	GCAGC	8 cut(s)	293, 508, 874, 877, 1732, 1801, 1930, 1933
LweI	GCATC	5 cut(s)	1003, 1283, 1370, 1691, 1811
MaeI	CTAG	3 cut(s)	126, 410, 1524
MaeII	ACGT	2 cut(s)	21, 256
MaeIII	GTNAC	5 cut(s)	488, 1261, 1628, 1859, 2082
MboII	GAAGA	8 cut(s)	64, 69, 324, 415, 654, 863, 926, 1403
MflI	RGATCY	2 cut(s)	329, 781
MhlI	GDGCHC	6 cut(s)	992, 1072, 1213, 1234, 1286, 1333
MlsI	TGGCCA	1 cut(s)	2004
MluNI	TGGCCA	1 cut(s)	2004
MlyI	GAGTC	4 cut(s)	61, 407, 1457, 1463
MmeI	TCCRAC	3 cut(s)	422, 479, 632
Mox20I	TGGCCA	1 cut(s)	2004
MscI	TGGCCA	1 cut(s)	2004
MseI	TTAA	3 cut(s)	297, 348, 1011
MslI	CAYNNNNRTG	7 cut(s)	509, 638, 1139, 1310, 1413, 1731, 1889
Msp20I	TGGCCA	1 cut(s)	2004
MspA1I	CMGCKG	4 cut(s)	307, 1280, 1745, 1924
MspI	CCGG	1 cut(s)	204
MspR9I	CCNGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
MvaI	CCWGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
MvnI	CGCG	3 cut(s)	307, 704, 1518
MwoI	GCNNNNNNNGC	8 cut(s)	287, 530, 588, 1217, 1508, 1758, 1782, 1979
NcoI	CCATGG	1 cut(s)	882
NdeI	CATATG	1 cut(s)	1714
NlaIV	GGNNCC	8 cut(s)	45, 202, 678, 1069, 1070, 1111, 1284, 1929
NmuCI	GTSAC	2 cut(s)	488, 1261
NruI	TCGCGA	1 cut(s)	1518
NspI	RCATGY	3 cut(s)	1144, 1673, 1792
PaeR7I	CTCGAG	1 cut(s)	156
PagI	TCATGA	2 cut(s)	1033, 1732
PasI	CCCWGGG	1 cut(s)	1936
PciI	ACATGT	1 cut(s)	1788
PfeI	GAWTC	5 cut(s)	119, 584, 878, 1340, 1612
PflMI	CCANNNNNTGG	1 cut(s)	461
PinAI	ACCGGT	1 cut(s)	203
PkrI	GCNGC	8 cut(s)	283, 523, 889, 892, 1747, 1816, 1920, 1923
Ple19I	CGATCG	1 cut(s)	1148
PleI	GAGTC	4 cut(s)	60, 407, 1456, 1462
PmaCI	CACGTG	1 cut(s)	257
PmlI	CACGTG	1 cut(s)	257
PpsI	GAGTC	4 cut(s)	60, 407, 1456, 1462
Ppu21I	YACGTR	1 cut(s)	257
PpuMI	RGGWCCY	1 cut(s)	1928
PscI	ACATGT	1 cut(s)	1788
Psp124BI	GAGCTC	1 cut(s)	1234
Psp1406I	AACGTT	1 cut(s)	21
Psp5II	RGGWCCY	1 cut(s)	1928
Psp6I	CCWGG	7 cut(s)	385, 494, 1935, 1963, 1968, 1999, 2122
PspCI	CACGTG	1 cut(s)	257
PspFI	CCCAGC	3 cut(s)	629, 1924, 1988
PspGI	CCWGG	7 cut(s)	385, 494, 1935, 1963, 1968, 1999, 2122
PspN4I	GGNNCC	8 cut(s)	45, 202, 678, 1069, 1070, 1111, 1284, 1929
PspOMI	GGGCCC	2 cut(s)	1068, 1282
PspPPI	RGGWCCY	1 cut(s)	1928
PstI	CTGCAG	1 cut(s)	781
PstNI	CAGNNNCTG	5 cut(s)	521, 776, 1184, 1924, 2123
PsuI	RGATCY	2 cut(s)	329, 781
PvuI	CGATCG	1 cut(s)	1148
PvuII	CAGCTG	1 cut(s)	1924
RruI	TCGCGA	1 cut(s)	1518
RsaI	GTAC	6 cut(s)	202, 242, 814, 904, 968, 1657
RsaNI	GTAC	6 cut(s)	201, 241, 813, 903, 967, 1656
RseI	CAYNNNNRTG	7 cut(s)	509, 638, 1139, 1310, 1413, 1731, 1889
SacI	GAGCTC	1 cut(s)	1234
SacII	CCGCGG	1 cut(s)	308
SaqAI	TTAA	3 cut(s)	297, 348, 1011
SatI	GCNGC	8 cut(s)	282, 522, 888, 891, 1746, 1815, 1919, 1922
ScaI	AGTACT	1 cut(s)	814
SchI	GAGTC	4 cut(s)	61, 407, 1457, 1463
ScrFI	CCNGG	7 cut(s)	387, 496, 1937, 1965, 1970, 2001, 2124
SduI	GDGCHC	6 cut(s)	992, 1072, 1213, 1234, 1286, 1333
SfaNI	GCATC	5 cut(s)	1003, 1283, 1370, 1691, 1811
SfcI	CTRYAG	1 cut(s)	777
Sfr274I	CTCGAG	1 cut(s)	156
Sfr303I	CCGCGG	1 cut(s)	308
SgrBI	CCGCGG	1 cut(s)	308
SinI	GGWCC	4 cut(s)	605, 1169, 1928, 2186
SlaI	CTCGAG	1 cut(s)	156
SmiMI	CAYNNNNRTG	7 cut(s)	509, 638, 1139, 1310, 1413, 1731, 1889
SmlI	CTYRAG	4 cut(s)	103, 156, 1256, 1694
SmoI	CTYRAG	4 cut(s)	103, 156, 1256, 1694
SsiI	CCGC	5 cut(s)	305, 307, 1167, 1280, 1743
SspMI	CTAG	3 cut(s)	126, 410, 1524
SstI	GAGCTC	1 cut(s)	1234
StyD4I	CCNGG	7 cut(s)	385, 494, 1935, 1963, 1968, 1999, 2122
StyI	CCWWGG	4 cut(s)	62, 882, 1622, 2182
TaaI	ACNGT	4 cut(s)	772, 802, 1660, 1859
TaiI	ACGT	2 cut(s)	24, 259
TaqI	TCGA	2 cut(s)	157, 1457
TaqII	GACCGA	1 cut(s)	351
TatI	WGTACW	3 cut(s)	240, 812, 1655
TfiI	GAWTC	5 cut(s)	119, 584, 878, 1340, 1612
Tru1I	TTAA	3 cut(s)	297, 348, 1011
Tru9I	TTAA	3 cut(s)	297, 348, 1011
TscAI	CASTG	4 cut(s)	411, 1552, 1665, 2071
TseFI	GTSAC	2 cut(s)	488, 1261
TseI	GCWGC	8 cut(s)	281, 521, 887, 890, 1745, 1814, 1918, 1921
Tsp45I	GTSAC	2 cut(s)	488, 1261
TspGWI	ACGGA	1 cut(s)	189
TspRI	CASTG	4 cut(s)	411, 1552, 1665, 2071
Van91I	CCANNNNNTGG	1 cut(s)	461
VneI	GTGCAC	3 cut(s)	988, 1209, 1329
VpaK11BI	GGWCC	4 cut(s)	605, 1169, 1928, 2186
XapI	RAATTY	3 cut(s)	433, 467, 499
XceI	RCATGY	3 cut(s)	1144, 1673, 1792
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1769
XhoI	CTCGAG	1 cut(s)	156
XmiI	GTMKAC	2 cut(s)	1356, 2154
XspI	CTAG	3 cut(s)	126, 410, 1524
ZrmI	AGTACT	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.