Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006318

Physical Location & Seq

Genomic Coordinates Forward (+)

5285993 .. 5289685

Gene Structure 3693 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1066200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1179 bp

ATGGCTCTTTTCTGCTCCCAATCTGCTGGTTCATGCTTAACTATTCTGTGCCTAGTTCTTGTTCAATGCCACTCTGCCATAATATACAGCATGAGGAACCACCACCACAACTTCCACCCTCACAGAACAGCTCCAATGTTCCAAACCAACCAATCCAGTTGCGCATTGTTTGCAGGCAATTGGGTCCAAGACGACTCCTACCCAATGTACCAATCCTCTGAGTGCCCCTTAATCATCGACCCAGAATTCAACTGCCAAATGTACGGCCGCCCGGACTCCGATTACCTCAAGTACAGATGGAGACCCCTCAATTGTGAGCTCCCAAGGTTCGATGGGGCTGAGTTTCTGCTCAAAATGAAAGGGAAAACAGTGATGTTTGTAGGGGATTCACTTGGGAAGAATCAATGGGAGTCTTTGGTTTGTCTCATTTACTCCGCAAATCCACAAACCCAGACTCAAATGGTCAAAGGAGTTCCACTTTCAACATTCAGTTTCCTGGACTACGGTGTGACACTGCAATTTTACAAAGCTCCGTACCTGGTCGACGTAGAAATTTATCAAGGGAAGAGAGTTCTGAAGCTTGAGGACATTTCGGAGAACGGCAAGGCGTGGCGGAATGCCGACGTTCTGTCGTTTAACACCGGTCATTGGTGGAGCCACGACGGAGGTCTCCAAGGGTGGGATTACATGGAGTCAGGAGGGAAATATTACAAAGATATGGACCGTTTGGCTGCTTTGGAGAAAGGGATGAAAACATGGGCTAATTGGGTTGATTCCAACATCGACACCTCACGGACCCGAGTTTTCTTCCTCTCCATTTCTCCCACACACTTCGACCCAAATGAATGGAATGGTGGTGCAACTCCTAGAACCACCAAGAATTGCTATGGAGAAACATCACCTATGAAAACGAGCGGAGTAACTTATCCCGGGACAACTTATCCAGACCAAATGAGAGTGATGGACAGTGTGATCCGGGACATGAGAAATCCTCCATCTTTGCTGGACATCACAATGCTTTCAGAGCTGAGAAAAGACGGGCACCCATCAATCTACAGTGGAGAATTAAGCCCTCAGCAGAGAGCCAATCCTGACCGTTCAGCAGATTGCAGCCATTGGTGCCTTCCTGGACTGCCAGACACTTGGAACCAACTCTTTTACACAACTTTGCTCTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0008150 GO:0008374 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

393

Amino Acids

44.8

Weight (kDa)

6.12

Isoelectric Point (pI)

38.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	53 - 106	8.9e-21	PMR5 N terminal Domain
PC-Esterase	PF13839	107 - 388	9.2e-92	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006318)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58600 AT5G58600
fragaria_vesca	FvH4_3g38960 FvH4_3g38960 FvH4_3g38960 FvH4_3g38960 FvH4_3g38960
malus_domestica	MD03G1066200.v1.1 MD11G1070900.v1.1
prunus_persica	Prupe.6G054900_v2.0.a1 Prupe.6G054900_v2.0.a1
pyrus_communis	pycom03g05290 pycom11g05930
rosa_chinensis	RchiOBHm_Chr5g0070191
rosa_laevigata	RLG00000036119
rosa_multiflora	Rmu_sc0002156.1_g000012
rosa_roxburghii	Rroxscaffold_1G00010920
rosa_rugosa	Rorug05G0403900 Rorug05G0403900 Rorug05G0404000 Rorug05G0404100 Rorug05G0404200
rosa_samantha	Rh5AG459700 Rh5BG478200 Rh5CG502200 Rh5DG490100
rosa_wichuraiana	Rw5G042880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	163
AccB1I	GGYRCC	2 cut(s)	1041, 1119
AccBSI	CCGCTC	1 cut(s)	915
AccI	GTMKAC	1 cut(s)	543
AciI	CCGC	4 cut(s)	268, 435, 613, 915
AclWI	GGATC	1 cut(s)	967
AcoI	YGGCCR	1 cut(s)	265
AcsI	RAATTY	2 cut(s)	245, 552
AcuI	CTGAAG	1 cut(s)	596
AfaI	GTAC	4 cut(s)	209, 263, 293, 536
AfiI	CCNNNNNNNGG	2 cut(s)	648, 679
AgeI	ACCGGT	1 cut(s)	641
AgsI	TTSAA	3 cut(s)	65, 250, 483
AhdI	GACNNNNNGTC	1 cut(s)	628
AjnI	CCWGG	3 cut(s)	495, 537, 1126
AluBI	AGCT	5 cut(s)	131, 319, 530, 580, 1027
AluI	AGCT	5 cut(s)	131, 319, 530, 580, 1027
Alw21I	GWGCWC	1 cut(s)	321
Alw26I	GTCTC	3 cut(s)	295, 428, 674
AlwI	GGATC	1 cut(s)	967
Ama87I	CYCGRG	2 cut(s)	798, 929
AoxI	GGCC	1 cut(s)	265
ApeKI	GCWGC	2 cut(s)	731, 1110
ApoI	RAATTY	2 cut(s)	245, 552
AsiGI	ACCGGT	1 cut(s)	641
AspLEI	GCGC	1 cut(s)	164
AspS9I	GGNCC	3 cut(s)	184, 721, 795
AsuC2I	CCSGG	4 cut(s)	272, 930, 931, 977
AsuHPI	GGTGA	1 cut(s)	891
AvaI	CYCGRG	2 cut(s)	798, 929
AvaII	GGWCC	3 cut(s)	184, 721, 795
BaeGI	GKGCMC	2 cut(s)	227, 1044
BanI	GGYRCC	2 cut(s)	1041, 1119
BanII	GRGCYC	1 cut(s)	321
Bbv12I	GWGCWC	1 cut(s)	321
BbvCI	CCTCAGC	1 cut(s)	1074
BbvI	GCAGC	2 cut(s)	718, 1122
BccI	CCATC	5 cut(s)	291, 326, 955, 1003, 1054
BceAI	ACGGC	2 cut(s)	280, 616
BciT130I	CCWGG	3 cut(s)	497, 539, 1128
BcnI	CCSGG	4 cut(s)	272, 930, 931, 977
BcoDI	GTCTC	3 cut(s)	295, 428, 674
BfaI	CTAG	2 cut(s)	53, 867
BfmI	CTRYAG	1 cut(s)	1054
BisI	GCNGC	3 cut(s)	268, 732, 1111
BlsI	GCNGC	3 cut(s)	269, 733, 1112
Bme1390I	CCNGG	7 cut(s)	272, 497, 539, 930, 931, 977, 1128
Bme18I	GGWCC	3 cut(s)	184, 721, 795
BmeRI	GACNNNNNGTC	1 cut(s)	628
BmeT110I	CYCGRG	2 cut(s)	798, 929
BmgT120I	GGNCC	3 cut(s)	184, 721, 795
BmiI	GGNNCC	7 cut(s)	98, 185, 656, 797, 1043, 1121, 1148
BmrFI	CCNGG	7 cut(s)	272, 497, 539, 930, 931, 977, 1128
BoxI	GACNNNNGTC	1 cut(s)	666
BplI	GAGNNNNNCTC	2 cut(s)	976, 1008
Bpu10I	CCTNAGC	1 cut(s)	1074
BpuEI	CTTGAG	2 cut(s)	272, 602
BpuMI	CCSGG	4 cut(s)	272, 930, 931, 977
BsaI	GGTCTC	2 cut(s)	295, 674
BsaJI	CCNNGG	3 cut(s)	323, 673, 929
BsaWI	WCCGGW	1 cut(s)	641
Bsc4I	CCNNNNNNNGG	2 cut(s)	648, 679
Bse118I	RCCGGY	1 cut(s)	641
Bse1I	ACTGG	1 cut(s)	156
BseBI	CCWGG	3 cut(s)	497, 539, 1128
BseDI	CCNNGG	3 cut(s)	323, 673, 929
BseGI	GGATG	1 cut(s)	753
BseLI	CCNNNNNNNGG	2 cut(s)	648, 679
BseMII	CTCAG	4 cut(s)	210, 330, 1019, 1088
BseNI	ACTGG	1 cut(s)	156
BseSI	GKGCMC	2 cut(s)	227, 1044
BseX3I	CGGCCG	1 cut(s)	265
BseXI	GCAGC	2 cut(s)	718, 1122
Bsh1285I	CGRYCG	1 cut(s)	268
BshFI	GGCC	1 cut(s)	267
BshNI	GGYRCC	2 cut(s)	1041, 1119
BshTI	ACCGGT	1 cut(s)	641
BsiEI	CGRYCG	1 cut(s)	268
BsiHKAI	GWGCWC	1 cut(s)	321
BsiHKCI	CYCGRG	2 cut(s)	798, 929
BsiSI	CCGG	4 cut(s)	272, 642, 930, 976
BslFI	GGGAC	2 cut(s)	946, 992
BslI	CCNNNNNNNGG	2 cut(s)	648, 679
BsmAI	GTCTC	3 cut(s)	295, 428, 674
BsmFI	GGGAC	2 cut(s)	946, 992
BsmI	GAATGC	1 cut(s)	622
BsnI	GGCC	1 cut(s)	267
Bso31I	GGTCTC	2 cut(s)	295, 674
BsoBI	CYCGRG	2 cut(s)	798, 929
Bsp1286I	GDGCHC	3 cut(s)	227, 321, 1044
Bsp143I	GATC	1 cut(s)	972
BspACI	CCGC	4 cut(s)	268, 435, 613, 915
BspANI	GGCC	1 cut(s)	267
BspCNI	CTCAG	4 cut(s)	211, 331, 1020, 1087
BspLI	GGNNCC	7 cut(s)	98, 185, 656, 797, 1043, 1121, 1148
BspPI	GGATC	1 cut(s)	967
BspT107I	GGYRCC	2 cut(s)	1041, 1119
BspTNI	GGTCTC	2 cut(s)	295, 674
BsrBI	CCGCTC	1 cut(s)	915
BsrFI	RCCGGY	1 cut(s)	641
BsrI	ACTGG	1 cut(s)	156
BssAI	RCCGGY	1 cut(s)	641
BssECI	CCNNGG	3 cut(s)	323, 673, 929
BssMI	GATC	1 cut(s)	972
BssT1I	CCWWGG	2 cut(s)	323, 673
Bst2UI	CCWGG	3 cut(s)	497, 539, 1128
Bst4CI	ACNGT	6 cut(s)	370, 506, 725, 968, 1058, 1097
Bst6I	CTCTTC	1 cut(s)	560
BstAPI	GCANNNNNTGC	1 cut(s)	170
BstC8I	GCNNGC	1 cut(s)	175
BstDEI	CTNAG	4 cut(s)	219, 339, 1028, 1074
BstF5I	GGATG	1 cut(s)	753
BstHHI	GCGC	1 cut(s)	164
BstKTI	GATC	1 cut(s)	975
BstMAI	GTCTC	3 cut(s)	295, 428, 674
BstMBI	GATC	1 cut(s)	972
BstMCI	CGRYCG	1 cut(s)	268
BstMWI	GCNNNNNNNGC	3 cut(s)	170, 1024, 1119
BstNI	CCWGG	3 cut(s)	497, 539, 1128
BstPAI	GACNNNNGTC	1 cut(s)	666
BstSCI	CCNGG	7 cut(s)	270, 495, 537, 928, 929, 975, 1126
BstSFI	CTRYAG	1 cut(s)	1054
BstSLI	GKGCMC	2 cut(s)	227, 1044
BstV1I	GCAGC	2 cut(s)	718, 1122
BstXI	CCANNNNNNTGG	3 cut(s)	26, 846, 1143
BstZI	CGGCCG	1 cut(s)	265
BsuRI	GGCC	1 cut(s)	267
BtsCI	GGATG	1 cut(s)	753
BtsI	GCAGTG	1 cut(s)	512
BtsIMutI	CAGTG	4 cut(s)	375, 512, 973, 1063
Cac8I	GCNNGC	1 cut(s)	175
CfoI	GCGC	1 cut(s)	164
Cfr10I	RCCGGY	1 cut(s)	641
Cfr13I	GGNCC	3 cut(s)	184, 721, 795
Cfr9I	CCCGGG	1 cut(s)	929
CsiI	ACCWGGT	1 cut(s)	537
Csp6I	GTAC	4 cut(s)	208, 262, 292, 535
CspAI	ACCGGT	1 cut(s)	641
CviAII	CATG	5 cut(s)	33, 91, 688, 756, 982
CviQI	GTAC	4 cut(s)	208, 262, 292, 535
DdeI	CTNAG	4 cut(s)	219, 339, 1028, 1074
DpnI	GATC	1 cut(s)	974
DpnII	GATC	1 cut(s)	972
DriI	GACNNNNNGTC	1 cut(s)	628
EaeI	YGGCCR	1 cut(s)	265
EagI	CGGCCG	1 cut(s)	265
Eam1104I	CTCTTC	1 cut(s)	560
Eam1105I	GACNNNNNGTC	1 cut(s)	628
EarI	CTCTTC	1 cut(s)	560
EciI	GGCGGA	1 cut(s)	628
Ecl136II	GAGCTC	1 cut(s)	319
EclXI	CGGCCG	1 cut(s)	265
Eco130I	CCWWGG	2 cut(s)	323, 673
Eco24I	GRGCYC	1 cut(s)	321
Eco31I	GGTCTC	2 cut(s)	295, 674
Eco47I	GGWCC	3 cut(s)	184, 721, 795
Eco52I	CGGCCG	1 cut(s)	265
Eco53kI	GAGCTC	1 cut(s)	319
Eco57I	CTGAAG	1 cut(s)	596
Eco88I	CYCGRG	2 cut(s)	798, 929
EcoICRI	GAGCTC	1 cut(s)	319
EcoRI	GAATTC	1 cut(s)	245
EcoRII	CCWGG	3 cut(s)	495, 537, 1126
EcoT14I	CCWWGG	2 cut(s)	323, 673
EcoT38I	GRGCYC	1 cut(s)	321
ErhI	CCWWGG	2 cut(s)	323, 673
FaeI	CATG	5 cut(s)	36, 94, 691, 759, 985
FaqI	GGGAC	2 cut(s)	946, 992
FatI	CATG	5 cut(s)	32, 90, 687, 755, 981
FblI	GTMKAC	1 cut(s)	543
Fnu4HI	GCNGC	3 cut(s)	268, 732, 1111
FokI	GGATG	1 cut(s)	760
FriOI	GRGCYC	1 cut(s)	321
Fsp4HI	GCNGC	3 cut(s)	268, 732, 1111
FspBI	CTAG	2 cut(s)	53, 867
FspI	TGCGCA	1 cut(s)	163
GlaI	GCGC	1 cut(s)	163
GluI	GCNGC	3 cut(s)	268, 732, 1111
HaeIII	GGCC	1 cut(s)	267
HapII	CCGG	4 cut(s)	272, 642, 930, 976
HhaI	GCGC	1 cut(s)	164
Hin1II	CATG	5 cut(s)	36, 94, 691, 759, 985
Hin6I	GCGC	1 cut(s)	162
HinP1I	GCGC	1 cut(s)	162
HincII	GTYRAC	1 cut(s)	544
HindII	GTYRAC	1 cut(s)	544
HindIII	AAGCTT	1 cut(s)	578
HinfI	GANTC	8 cut(s)	194, 275, 386, 400, 410, 454, 692, 773
HpaII	CCGG	4 cut(s)	272, 642, 930, 976
HphI	GGTGA	1 cut(s)	891
Hpy166II	GTNNAC	1 cut(s)	544
Hpy188I	TCNGA	6 cut(s)	220, 280, 576, 595, 1024, 1178
Hpy188III	TCNNGA	3 cut(s)	696, 944, 1091
Hpy8I	GTNNAC	1 cut(s)	544
Hpy99I	CGWCG	3 cut(s)	548, 626, 665
HpyAV	CCTTC	1 cut(s)	1133
HpyCH4III	ACNGT	6 cut(s)	370, 506, 725, 968, 1058, 1097
HpyCH4IV	ACGT	2 cut(s)	546, 624
HpyCH4V	TGCA	4 cut(s)	173, 517, 860, 1110
HpyF10VI	GCNNNNNNNGC	3 cut(s)	170, 1024, 1119
HpyF3I	CTNAG	4 cut(s)	219, 339, 1028, 1074
HpySE526I	ACGT	2 cut(s)	546, 624
Hsp92II	CATG	5 cut(s)	36, 94, 691, 759, 985
HspAI	GCGC	1 cut(s)	162
Kzo9I	GATC	1 cut(s)	972
LmnI	GCTCC	5 cut(s)	20, 136, 324, 535, 654
Lsp1109I	GCAGC	2 cut(s)	718, 1122
MabI	ACCWGGT	1 cut(s)	537
MaeI	CTAG	2 cut(s)	53, 867
MaeII	ACGT	2 cut(s)	546, 624
MaeIII	GTNAC	2 cut(s)	508, 919
MalI	GATC	1 cut(s)	974
MbiI	CCGCTC	1 cut(s)	915
MboI	GATC	1 cut(s)	972
MboII	GAAGA	4 cut(s)	409, 577, 799, 1165
MfeI	CAATTG	2 cut(s)	178, 310
MhlI	GDGCHC	3 cut(s)	227, 321, 1044
MluCI	AATT	8 cut(s)	178, 245, 310, 518, 552, 763, 880, 1064
MlyI	GAGTC	5 cut(s)	188, 269, 419, 448, 701
MmeI	TCCRAC	1 cut(s)	801
MseI	TTAA	4 cut(s)	38, 230, 636, 1067
MslI	CAYNNNNRTG	1 cut(s)	1013
MspI	CCGG	4 cut(s)	272, 642, 930, 976
MspR9I	CCNGG	7 cut(s)	272, 497, 539, 930, 931, 977, 1128
MunI	CAATTG	2 cut(s)	178, 310
Mva1269I	GAATGC	1 cut(s)	622
MvaI	CCWGG	3 cut(s)	497, 539, 1128
MwoI	GCNNNNNNNGC	3 cut(s)	170, 1024, 1119
NciI	CCSGG	4 cut(s)	272, 930, 931, 977
NdeII	GATC	1 cut(s)	972
NlaIII	CATG	5 cut(s)	36, 94, 691, 759, 985
NlaIV	GGNNCC	7 cut(s)	98, 185, 656, 797, 1043, 1121, 1148
NmuCI	GTSAC	1 cut(s)	508
NsbI	TGCGCA	1 cut(s)	163
PctI	GAATGC	1 cut(s)	622
PfeI	GAWTC	3 cut(s)	386, 400, 773
PfoI	TCCNGGA	3 cut(s)	495, 975, 1126
PinAI	ACCGGT	1 cut(s)	641
PkrI	GCNGC	3 cut(s)	269, 733, 1112
PleI	GAGTC	5 cut(s)	188, 269, 418, 448, 700
PpsI	GAGTC	5 cut(s)	188, 269, 418, 448, 700
PshAI	GACNNNNGTC	1 cut(s)	666
Psp124BI	GAGCTC	1 cut(s)	321
Psp6I	CCWGG	3 cut(s)	495, 537, 1126
PspGI	CCWGG	3 cut(s)	495, 537, 1126
PspN4I	GGNNCC	7 cut(s)	98, 185, 656, 797, 1043, 1121, 1148
PspPI	GGNCC	3 cut(s)	184, 721, 795
RsaI	GTAC	4 cut(s)	209, 263, 293, 536
RsaNI	GTAC	4 cut(s)	208, 262, 292, 535
RseI	CAYNNNNRTG	1 cut(s)	1013
SacI	GAGCTC	1 cut(s)	321
SalI	GTCGAC	1 cut(s)	542
SaqAI	TTAA	4 cut(s)	38, 230, 636, 1067
SatI	GCNGC	3 cut(s)	268, 732, 1111
Sau3AI	GATC	1 cut(s)	972
Sau96I	GGNCC	3 cut(s)	184, 721, 795
SchI	GAGTC	5 cut(s)	188, 269, 419, 448, 701
ScrFI	CCNGG	7 cut(s)	272, 497, 539, 930, 931, 977, 1128
SduI	GDGCHC	3 cut(s)	227, 321, 1044
SexAI	ACCWGGT	1 cut(s)	537
SfcI	CTRYAG	1 cut(s)	1054
SinI	GGWCC	3 cut(s)	184, 721, 795
SmaI	CCCGGG	1 cut(s)	931
SmiMI	CAYNNNNRTG	1 cut(s)	1013
SmlI	CTYRAG	2 cut(s)	287, 581
SmoI	CTYRAG	2 cut(s)	287, 581
Sse9I	AATT	8 cut(s)	178, 245, 310, 518, 552, 763, 880, 1064
SsiI	CCGC	4 cut(s)	268, 435, 613, 915
SspI	AATATT	1 cut(s)	707
SspMI	CTAG	2 cut(s)	53, 867
SstI	GAGCTC	1 cut(s)	321
StyD4I	CCNGG	7 cut(s)	270, 495, 537, 928, 929, 975, 1126
StyI	CCWWGG	2 cut(s)	323, 673
TaaI	ACNGT	6 cut(s)	370, 506, 725, 968, 1058, 1097
TaiI	ACGT	2 cut(s)	549, 627
TaqI	TCGA	5 cut(s)	237, 330, 543, 783, 834
TasI	AATT	8 cut(s)	178, 245, 310, 518, 552, 763, 880, 1064
TatI	WGTACW	1 cut(s)	291
TauI	GCSGC	1 cut(s)	270
TfiI	GAWTC	3 cut(s)	386, 400, 773
Tru1I	TTAA	4 cut(s)	38, 230, 636, 1067
Tru9I	TTAA	4 cut(s)	38, 230, 636, 1067
TscAI	CASTG	4 cut(s)	375, 519, 973, 1063
TseFI	GTSAC	1 cut(s)	508
TseI	GCWGC	2 cut(s)	731, 1110
Tsp45I	GTSAC	1 cut(s)	508
TspDTI	ATGAA	5 cut(s)	21, 371, 764, 858, 920
TspGWI	ACGGA	3 cut(s)	522, 678, 808
TspMI	CCCGGG	1 cut(s)	929
TspRI	CASTG	4 cut(s)	375, 519, 973, 1063
VpaK11BI	GGWCC	3 cut(s)	184, 721, 795
XapI	RAATTY	2 cut(s)	245, 552
XmaI	CCCGGG	1 cut(s)	929
XmiI	GTMKAC	1 cut(s)	543
XspI	CTAG	2 cut(s)	53, 867

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.