Rh5BG478200

GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006318
Physical Location & Seq
Forward (+)
76081811 .. 76084155
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG478200.1

Sequence Viewer

Length: 678 bp
ATGTATGGGCGCCCAGATTCTGAGTACCTCAAATATAGATGGAGACCCCTCAATTGTGAACTCCCAAGGTTCAATGGGGTTGAGTTTTTGCTCAGAATGAAAGGGAAAACAGTGATGTTTGTAGGTGATTCACTTGGGAGGAATCAATGGGAGTCTTTGGTTTGCTTGATATACTCCGCAGCTCCCCAAACCCAAACCCAAATGGTGAAAGGAGATCCACTCTCAACATTCAACTTCTTGGACTATGGTGTAACCCTGCAATACTACCGAGCTCCATATCTGGTGGACATCGATATGATGGAAGGAAAGAGAGTTCTGAGGCTTGAGGACATATCGGAGAACAGCAATGCGTGGCGTTCCGCCGATGTGCTGTCGTTTAACACCGGTCACTGGTGGAGTCACACCGGAGGTCTCCAAGGGTGGGATTACATGGAATCAGGAGGAAAATATTACAAAGATATGGATCGTTTGGCTGCTTTGGAGAAAGGAATGAGAACCTGGGCAAATTGGGTCGACAAAAACATCGATACCACAAGGACCCGAGTTTTCTTTCTCTCCATATCTCCCACACATTACGACCCAAATGAATGGAATGGGGGAGCAACACCTACAACCACCAAGAACTGCTATGGAGAAACATCACCGATGACAGTGAGTGGGATAACTTATCCGGGGACG

Protein Analysis

226

Amino Acids

26.02

Weight (kDa)

6.31

Isoelectric Point (pI)

29.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 21 - 219 2.3e-62 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 9
AccI GTMKAC 1 cut(s) 513
AciI CCGC 2 cut(s) 177, 360
AclWI GGATC 2 cut(s) 209, 471
AcyI GRCGYC 1 cut(s) 10
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 390, 421
AgeI ACCGGT 1 cut(s) 383
AgsI TTSAA 2 cut(s) 73, 232
AjnI CCWGG 1 cut(s) 497
AloI GAACNNNNNNTCC 2 cut(s) 297, 329
AluBI AGCT 2 cut(s) 182, 272
AluI AGCT 2 cut(s) 182, 272
Alw21I GWGCWC 1 cut(s) 274
Alw26I GTCTC 2 cut(s) 37, 416
AlwI GGATC 2 cut(s) 209, 471
AlwNI CAGNNNCTG 1 cut(s) 20
Ama87I CYCGRG 1 cut(s) 540
ApeKI GCWGC 2 cut(s) 179, 473
AsiGI ACCGGT 1 cut(s) 383
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 537
AsuC2I CCSGG 1 cut(s) 672
AsuHPI GGTGA 3 cut(s) 137, 217, 633
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 1 cut(s) 537
BanI GGYRCC 1 cut(s) 9
BanII GRGCYC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 2 cut(s) 191, 460
BccI CCATC 2 cut(s) 33, 292
BciT130I CCWGG 1 cut(s) 499
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 2 cut(s) 37, 416
BfoI RGCGCY 1 cut(s) 13
BisI GCNGC 2 cut(s) 180, 474
BlsI GCNGC 2 cut(s) 181, 475
Bme1390I CCNGG 2 cut(s) 499, 672
Bme18I GGWCC 1 cut(s) 537
BmeT110I CYCGRG 1 cut(s) 540
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 2 cut(s) 11, 539
BmrFI CCNGG 2 cut(s) 499, 672
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpuEI CTTGAG 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 672
Bsa29I ATCGAT 2 cut(s) 291, 525
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 10
BsaI GGTCTC 2 cut(s) 37, 416
BsaJI CCNNGG 4 cut(s) 65, 415, 498, 671
BsaWI WCCGGW 2 cut(s) 383, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 421
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 1 cut(s) 395
Bse3DI GCAATG 1 cut(s) 352
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 1 cut(s) 499
BseCI ATCGAT 2 cut(s) 291, 525
BseDI CCNNGG 4 cut(s) 65, 415, 498, 671
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 390, 421
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 3 cut(s) 12, 106, 308
BseNI ACTGG 1 cut(s) 395
BseXI GCAGC 2 cut(s) 191, 460
BshNI GGYRCC 1 cut(s) 9
BshTI ACCGGT 1 cut(s) 383
BshVI ATCGAT 2 cut(s) 291, 525
BsiHKAI GWGCWC 1 cut(s) 274
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 3 cut(s) 384, 405, 671
BslI CCNNNNNNNGG 2 cut(s) 390, 421
BsmAI GTCTC 2 cut(s) 37, 416
Bso31I GGTCTC 2 cut(s) 37, 416
BsoBI CYCGRG 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 274
Bsp143I GATC 2 cut(s) 214, 463
BspACI CCGC 2 cut(s) 177, 360
BspCNI CTCAG 3 cut(s) 13, 105, 309
BspDI ATCGAT 2 cut(s) 291, 525
BspLI GGNNCC 2 cut(s) 11, 539
BspPI GGATC 2 cut(s) 209, 471
BspT107I GGYRCC 1 cut(s) 9
BspTNI GGTCTC 2 cut(s) 37, 416
BsrDI GCAATG 1 cut(s) 352
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 4 cut(s) 65, 415, 498, 671
BssMI GATC 2 cut(s) 214, 463
BssNI GRCGYC 1 cut(s) 10
BssT1I CCWWGG 2 cut(s) 65, 415
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 2 cut(s) 112, 652
BstACI GRCGYC 1 cut(s) 10
BstDEI CTNAG 3 cut(s) 21, 92, 317
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 2 cut(s) 217, 466
BstMAI GTCTC 2 cut(s) 37, 416
BstMBI GATC 2 cut(s) 214, 463
BstNI CCWGG 1 cut(s) 499
BstSCI CCNGG 2 cut(s) 497, 670
BstV1I GCAGC 2 cut(s) 191, 460
BstX2I RGATCY 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 588
BstYI RGATCY 1 cut(s) 214
Bsu15I ATCGAT 2 cut(s) 291, 525
BsuTUI ATCGAT 2 cut(s) 291, 525
BtsIMutI CAGTG 3 cut(s) 117, 388, 657
CaiI CAGNNNCTG 1 cut(s) 20
CfoI GCGC 1 cut(s) 12
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 537
ClaI ATCGAT 2 cut(s) 291, 525
Csp6I GTAC 1 cut(s) 25
CspAI ACCGGT 1 cut(s) 383
CviAII CATG 1 cut(s) 430
CviJI RGCY 4 cut(s) 182, 272, 322, 473
CviKI_1 RGCY 4 cut(s) 182, 272, 322, 473
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 3 cut(s) 21, 92, 317
DinI GGCGCC 1 cut(s) 11
DpnI GATC 2 cut(s) 216, 465
DpnII GATC 2 cut(s) 214, 463
EciI GGCGGA 1 cut(s) 349
Ecl136II GAGCTC 1 cut(s) 272
Eco130I CCWWGG 2 cut(s) 65, 415
Eco24I GRGCYC 1 cut(s) 274
Eco31I GGTCTC 2 cut(s) 37, 416
Eco47I GGWCC 1 cut(s) 537
Eco53kI GAGCTC 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 540
EcoICRI GAGCTC 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 537
EcoRII CCWGG 1 cut(s) 497
EcoT14I CCWWGG 2 cut(s) 65, 415
EcoT38I GRGCYC 1 cut(s) 274
EgeI GGCGCC 1 cut(s) 11
EheI GGCGCC 1 cut(s) 11
ErhI CCWWGG 2 cut(s) 65, 415
FaeI CATG 1 cut(s) 433
FatI CATG 1 cut(s) 429
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 2 cut(s) 180, 474
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 2 cut(s) 180, 474
GlaI GCGC 1 cut(s) 11
GluI GCNGC 2 cut(s) 180, 474
HaeII RGCGCY 1 cut(s) 13
HapII CCGG 3 cut(s) 384, 405, 671
HhaI GCGC 1 cut(s) 12
Hin1I GRCGYC 1 cut(s) 10
Hin1II CATG 1 cut(s) 433
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HinfI GANTC 6 cut(s) 17, 128, 142, 152, 397, 434
HpaII CCGG 3 cut(s) 384, 405, 671
HphI GGTGA 3 cut(s) 137, 217, 633
Hpy166II GTNNAC 3 cut(s) 59, 286, 514
Hpy188I TCNGA 4 cut(s) 22, 95, 318, 337
Hpy188III TCNNGA 1 cut(s) 438
Hpy8I GTNNAC 3 cut(s) 59, 286, 514
HpyAV CCTTC 1 cut(s) 296
HpyCH4III ACNGT 2 cut(s) 112, 652
HpyCH4V TGCA 1 cut(s) 259
HpyF3I CTNAG 3 cut(s) 21, 92, 317
Hsp92I GRCGYC 1 cut(s) 10
Hsp92II CATG 1 cut(s) 433
HspAI GCGC 1 cut(s) 10
KasI GGCGCC 1 cut(s) 9
Kzo9I GATC 2 cut(s) 214, 463
LmnI GCTCC 3 cut(s) 187, 277, 599
LpnPI CCDG 9 cut(s) 27, 266, 269, 376, 397, 418, 423, 484, 511
Lsp1109I GCAGC 2 cut(s) 191, 460
MaeIII GTNAC 3 cut(s) 250, 386, 398
MalI GATC 2 cut(s) 216, 465
MboI GATC 2 cut(s) 214, 463
MfeI CAATTG 1 cut(s) 52
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 1 cut(s) 274
MluCI AATT 2 cut(s) 52, 505
Mly113I GGCGCC 1 cut(s) 10
MlyI GAGTC 2 cut(s) 161, 406
MnlI CCTC 7 cut(s) 38, 59, 132, 312, 319, 401, 434
MseI TTAA 1 cut(s) 378
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 3 cut(s) 384, 405, 671
MspR9I CCNGG 2 cut(s) 499, 672
MunI CAATTG 1 cut(s) 52
MvaI CCWGG 1 cut(s) 499
NarI GGCGCC 1 cut(s) 10
NciI CCSGG 1 cut(s) 672
NdeII GATC 2 cut(s) 214, 463
NlaIII CATG 1 cut(s) 433
NlaIV GGNNCC 2 cut(s) 11, 539
NmuCI GTSAC 2 cut(s) 386, 398
PfeI GAWTC 4 cut(s) 17, 128, 142, 434
PinAI ACCGGT 1 cut(s) 383
PkrI GCNGC 2 cut(s) 181, 475
PleI GAGTC 2 cut(s) 160, 405
PluTI GGCGCC 1 cut(s) 13
PpsI GAGTC 2 cut(s) 160, 405
PpuMI RGGWCCY 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 497
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 2 cut(s) 11, 539
PspPI GGNCC 1 cut(s) 537
PspPPI RGGWCCY 1 cut(s) 537
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 1 cut(s) 293
SacI GAGCTC 1 cut(s) 274
SalI GTCGAC 1 cut(s) 512
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 2 cut(s) 180, 474
Sau3AI GATC 2 cut(s) 214, 463
Sau96I GGNCC 1 cut(s) 537
SchI GAGTC 2 cut(s) 161, 406
ScrFI CCNGG 2 cut(s) 499, 672
SduI GDGCHC 1 cut(s) 274
SetI ASST 8 cut(s) 30, 71, 127, 184, 274, 412, 500, 610
SfoI GGCGCC 1 cut(s) 11
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 2 cut(s) 52, 505
SsiI CCGC 2 cut(s) 177, 360
SspDI GGCGCC 1 cut(s) 9
SspI AATATT 1 cut(s) 449
SstI GAGCTC 1 cut(s) 274
StyD4I CCNGG 2 cut(s) 497, 670
StyI CCWWGG 2 cut(s) 65, 415
TaaI ACNGT 2 cut(s) 112, 652
TaqI TCGA 3 cut(s) 291, 513, 525
TasI AATT 2 cut(s) 52, 505
TfiI GAWTC 4 cut(s) 17, 128, 142, 434
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TscAI CASTG 3 cut(s) 117, 395, 657
TseFI GTSAC 2 cut(s) 386, 398
TseI GCWGC 2 cut(s) 179, 473
Tsp45I GTSAC 2 cut(s) 386, 398
TspDTI ATGAA 2 cut(s) 113, 600
TspRI CASTG 3 cut(s) 117, 395, 657
VpaK11BI GGWCC 1 cut(s) 537
XmiI GTMKAC 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.