Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012155

Physical Location & Seq

Genomic Coordinates Reverse (-)

5313590 .. 5315802

Gene Structure 2213 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1066600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGATAATTTTTTTTTTTTTCCAAATTGCTATGATAATTAATAAGCAGCCTCCTGCCCAACAAGAAGACGATGACCTCCGTTGTTCTGAGAAAAAAATGGCTTCTGAATCCTACCCTCCACCGTTTCGTTCACTTCCAGATTCAACCCAGGCCGTTGATTTGGACGGCCAAGTCAACCCGGTTCATGATTCGGAGGATCCGATTCCTGTGGTGGATCTCCAGAGCCTGAAACTTGGCGAGGCATGCGAGTTTTGGGGAATTTTTCGTTTGGTCAATCATGGAGTTCCTCCAACCCTTTTGACCCAACTTCGGGAGCATGCCAAAATGGTTTTTTCCCTGCCGTTTGAGTCCAAACAGAGCCTTATCACCAACCCTCTGTCCTACTTCTGGGGTACCCCTGCCCTTACTCCATCTGGGGCGGCCTTATCAACAAAGGGTAGTAGTACCCTCCAGAATATCAACTGGGTTGAAGGACTCAACGTCCCTCTAGCTCAACTCAGTTTTCAAATTGACAATCCCATAATTGCTTCTTTCAGCCTTGTGCTAGAAGAATATGGGAAGCACCTAGCTAGGCTAGCGACAACTATATTTGACGCTATGGTAATAAACCTTGGATTGGATCCAATAGAATCCAAATCCCATTTATCAAATTCTACTGGCATTGTACGTGTCTATCGCTACCCACCACATTGCTCCAACTCCAGCTCTGCATCTGCATGGGGCATGGACGTGCACACTGACAGCTCTGTGCTCTCGATACTAAATCAAGATGAAGTCGGTGGACTCGAAGTTCTCAAAGACAATGAGTGGTTCAGTGTTAACCCAATTCCCAACACACTGATTGTCAACCTCGGAGACATGATGCAGGCGATAAGCGATGACAAGTACAAGAGTGTGAAGCACAGAGTGAAGGTGAACAGAAGCAAAGAGAGAGTTTCAATCTGCTATTTTGTGTTTCCGGGAGAAGGCAGTGTGATTCGGAGCTCAAACTATAAGCCCTTTACTTATAGGGATTTTCAGAACGAAGTGCAGCAAGATATAAAGAGTGTTGGGTATAAGGTCGGGCTTGAGAGGTTCCAAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0009685 GO:0009987 GO:0010817 GO:0016101 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0042445 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0045543 GO:0051213 GO:0052635 GO:0055114 GO:0065007 GO:0065008 GO:0071704

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

362

Amino Acids

40.34

Weight (kDa)

5.42

Isoelectric Point (pI)

37.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	76 - 158	1.2e-12	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	222 - 320	1.9e-29	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012155)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58660 AT5G58660
fragaria_vesca	FvH4_3g38920
malus_domestica	MD03G1066600.v1.1 MD11G1071600.v1.1
prunus_persica	Prupe.6G055300_v2.0.a1
pyrus_communis	pycom03g05320 pycom11g05970
rosa_chinensis	RchiOBHm_Chr5g0070171
rosa_laevigata	RLG00000036117
rosa_multiflora	Rmu_sc0013014.1_g000007 Rmu_sc0018909.1_g000006
rosa_roxburghii	Rroxscaffold_1G00010950
rosa_rugosa	Rorug05G0403600
rosa_samantha	Rh5AG459500 Rh5BG478000 Rh5CG501900 Rh5DG489900
rosa_wichuraiana	Rw5G042860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	170
Acc65I	GGTACC	1 cut(s)	392
AccB1I	GGYRCC	1 cut(s)	392
AciI	CCGC	1 cut(s)	419
AclWI	GGATC	5 cut(s)	191, 204, 222, 614, 627
AcoI	YGGCCR	1 cut(s)	166
AcsI	RAATTY	2 cut(s)	258, 649
AdeI	CACNNNGTG	1 cut(s)	907
AfaI	GTAC	4 cut(s)	394, 445, 666, 887
AfiI	CCNNNNNNNGG	5 cut(s)	309, 310, 387, 616, 965
AflIII	ACRYGT	1 cut(s)	667
AgsI	TTSAA	4 cut(s)	144, 470, 506, 939
AhdI	GACNNNNNGTC	1 cut(s)	479
AjiI	CACGTC	1 cut(s)	730
AjnI	CCWGG	1 cut(s)	147
AloI	GAACNNNNNNTCC	2 cut(s)	774, 806
AluBI	AGCT	6 cut(s)	491, 569, 705, 744, 984, 1082
AluI	AGCT	6 cut(s)	491, 569, 705, 744, 984, 1082
Alw21I	GWGCWC	3 cut(s)	735, 753, 986
Alw26I	GTCTC	1 cut(s)	849
Alw44I	GTGCAC	1 cut(s)	731
AlwI	GGATC	5 cut(s)	191, 204, 222, 614, 627
AlwNI	CAGNNNCTG	1 cut(s)	226
AoxI	GGCC	3 cut(s)	150, 166, 420
ApaLI	GTGCAC	1 cut(s)	731
ApeKI	GCWGC	2 cut(s)	46, 1030
ApoI	RAATTY	2 cut(s)	258, 649
AseI	ATTAAT	1 cut(s)	39
Asp718I	GGTACC	1 cut(s)	392
AsuC2I	CCSGG	2 cut(s)	179, 960
AsuHPI	GGTGA	2 cut(s)	358, 925
AsuNHI	GCTAGC	1 cut(s)	574
BaeGI	GKGCMC	1 cut(s)	735
BamHI	GGATCC	2 cut(s)	196, 619
BanI	GGYRCC	1 cut(s)	392
BanII	GRGCYC	1 cut(s)	986
BbsI	GAAGAC	1 cut(s)	72
Bbv12I	GWGCWC	3 cut(s)	735, 753, 986
BbvI	GCAGC	2 cut(s)	58, 1042
BccI	CCATC	1 cut(s)	418
BceAI	ACGGC	3 cut(s)	137, 181, 325
BciT130I	CCWGG	1 cut(s)	149
BcnI	CCSGG	2 cut(s)	179, 960
BcoDI	GTCTC	1 cut(s)	849
BfaI	CTAG	5 cut(s)	488, 545, 566, 570, 575
BisI	GCNGC	3 cut(s)	47, 420, 1031
BlsI	GCNGC	3 cut(s)	48, 421, 1032
Bme1390I	CCNGG	3 cut(s)	149, 179, 960
BmeRI	GACNNNNNGTC	1 cut(s)	479
BmgBI	CACGTC	1 cut(s)	730
BmiI	GGNNCC	4 cut(s)	198, 394, 621, 1076
BmrFI	CCNGG	3 cut(s)	149, 179, 960
BmrI	ACTGGG	1 cut(s)	472
BmsI	GCATC	2 cut(s)	719, 852
BmtI	GCTAGC	1 cut(s)	578
BmuI	ACTGGG	1 cut(s)	472
BpiI	GAAGAC	1 cut(s)	72
BpmI	CTGGAG	3 cut(s)	203, 434, 685
BpuMI	CCSGG	2 cut(s)	179, 960
BsaAI	YACGTR	1 cut(s)	668
BsaJI	CCNNGG	3 cut(s)	147, 610, 850
Bsc4I	CCNNNNNNNGG	5 cut(s)	309, 310, 387, 616, 965
Bse1I	ACTGG	2 cut(s)	467, 661
Bse3DI	GCAATG	1 cut(s)	688
BseBI	CCWGG	1 cut(s)	149
BseDI	CCNNGG	3 cut(s)	147, 610, 850
BseLI	CCNNNNNNNGG	5 cut(s)	309, 310, 387, 616, 965
BseMI	GCAATG	1 cut(s)	688
BseMII	CTCAG	2 cut(s)	78, 511
BseNI	ACTGG	2 cut(s)	467, 661
BseSI	GKGCMC	1 cut(s)	735
BseXI	GCAGC	2 cut(s)	58, 1042
BsgI	GTGCAG	1 cut(s)	1049
BshFI	GGCC	3 cut(s)	152, 168, 422
BshNI	GGYRCC	1 cut(s)	392
BsiHKAI	GWGCWC	3 cut(s)	735, 753, 986
BsiSI	CCGG	2 cut(s)	179, 959
BslFI	GGGAC	1 cut(s)	467
BslI	CCNNNNNNNGG	5 cut(s)	309, 310, 387, 616, 965
BsmAI	GTCTC	1 cut(s)	849
BsmFI	GGGAC	1 cut(s)	467
BsnI	GGCC	3 cut(s)	152, 168, 422
Bsp1286I	GDGCHC	3 cut(s)	735, 753, 986
Bsp143I	GATC	3 cut(s)	196, 214, 619
BspACI	CCGC	1 cut(s)	419
BspANI	GGCC	3 cut(s)	152, 168, 422
BspCNI	CTCAG	2 cut(s)	79, 510
BspHI	TCATGA	1 cut(s)	184
BspLI	GGNNCC	4 cut(s)	198, 394, 621, 1076
BspOI	GCTAGC	1 cut(s)	578
BspPI	GGATC	5 cut(s)	191, 204, 222, 614, 627
BspT107I	GGYRCC	1 cut(s)	392
BsrDI	GCAATG	1 cut(s)	688
BsrI	ACTGG	2 cut(s)	467, 661
BssECI	CCNNGG	3 cut(s)	147, 610, 850
BssMI	GATC	3 cut(s)	196, 214, 619
BssT1I	CCWWGG	1 cut(s)	610
Bst2UI	CCWGG	1 cut(s)	149
Bst4CI	ACNGT	1 cut(s)	123
BstBAI	YACGTR	1 cut(s)	668
BstC8I	GCNNGC	4 cut(s)	244, 318, 576, 867
BstDEI	CTNAG	3 cut(s)	87, 497, 1083
BstKTI	GATC	3 cut(s)	199, 217, 622
BstMAI	GTCTC	1 cut(s)	849
BstMBI	GATC	3 cut(s)	196, 214, 619
BstMWI	GCNNNNNNNGC	2 cut(s)	243, 575
BstNI	CCWGG	1 cut(s)	149
BstNSI	RCATGY	2 cut(s)	246, 320
BstSCI	CCNGG	3 cut(s)	147, 177, 958
BstSLI	GKGCMC	1 cut(s)	735
BstV1I	GCAGC	2 cut(s)	58, 1042
BstV2I	GAAGAC	1 cut(s)	72
BstX2I	RGATCY	3 cut(s)	196, 214, 619
BstYI	RGATCY	3 cut(s)	196, 214, 619
BsuRI	GGCC	3 cut(s)	152, 168, 422
BtgZI	GCGATG	1 cut(s)	891
BtrI	CACGTC	1 cut(s)	730
BtsI	GCAGTG	1 cut(s)	976
BtsIMutI	CAGTG	4 cut(s)	735, 820, 836, 976
Cac8I	GCNNGC	4 cut(s)	244, 318, 576, 867
CaiI	CAGNNNCTG	1 cut(s)	226
CciI	TCATGA	1 cut(s)	184
CseI	GACGC	1 cut(s)	602
Csp6I	GTAC	4 cut(s)	393, 444, 665, 886
CviAII	CATG	7 cut(s)	185, 243, 278, 317, 717, 724, 859
CviQI	GTAC	4 cut(s)	393, 444, 665, 886
DdeI	CTNAG	3 cut(s)	87, 497, 1083
DpnI	GATC	3 cut(s)	198, 216, 621
DpnII	GATC	3 cut(s)	196, 214, 619
DraIII	CACNNNGTG	1 cut(s)	907
DrdI	GACNNNNNNGTC	1 cut(s)	170
DriI	GACNNNNNGTC	1 cut(s)	479
DseDI	GACNNNNNNGTC	1 cut(s)	170
EaeI	YGGCCR	1 cut(s)	166
Eam1105I	GACNNNNNGTC	1 cut(s)	479
Ecl136II	GAGCTC	1 cut(s)	984
Eco130I	CCWWGG	1 cut(s)	610
Eco24I	GRGCYC	1 cut(s)	986
Eco53kI	GAGCTC	1 cut(s)	984
EcoICRI	GAGCTC	1 cut(s)	984
EcoRII	CCWGG	1 cut(s)	147
EcoT14I	CCWWGG	1 cut(s)	610
EcoT38I	GRGCYC	1 cut(s)	986
ErhI	CCWWGG	1 cut(s)	610
FaeI	CATG	7 cut(s)	188, 246, 281, 320, 720, 727, 862
FaqI	GGGAC	1 cut(s)	467
FatI	CATG	7 cut(s)	184, 242, 277, 316, 716, 723, 858
Fnu4HI	GCNGC	3 cut(s)	47, 420, 1031
FriOI	GRGCYC	1 cut(s)	986
Fsp4HI	GCNGC	3 cut(s)	47, 420, 1031
FspBI	CTAG	5 cut(s)	488, 545, 566, 570, 575
GluI	GCNGC	3 cut(s)	47, 420, 1031
GsuI	CTGGAG	3 cut(s)	203, 434, 685
HaeIII	GGCC	3 cut(s)	152, 168, 422
HapII	CCGG	2 cut(s)	179, 959
HgaI	GACGC	1 cut(s)	602
Hin1II	CATG	7 cut(s)	188, 246, 281, 320, 720, 727, 862
HincII	GTYRAC	3 cut(s)	175, 820, 847
HindII	GTYRAC	3 cut(s)	175, 820, 847
HindIII	AAGCTT	1 cut(s)	1080
HinfI	GANTC	9 cut(s)	107, 140, 188, 202, 347, 474, 629, 783, 976
HpaI	GTTAAC	1 cut(s)	820
HpaII	CCGG	2 cut(s)	179, 959
HphI	GGTGA	2 cut(s)	358, 925
Hpy166II	GTNNAC	7 cut(s)	131, 175, 733, 782, 820, 847, 916
Hpy188I	TCNGA	7 cut(s)	88, 106, 193, 201, 854, 981, 1020
Hpy188III	TCNNGA	7 cut(s)	137, 185, 220, 311, 451, 754, 767
Hpy8I	GTNNAC	7 cut(s)	131, 175, 733, 782, 820, 847, 916
HpyAV	CCTTC	3 cut(s)	464, 904, 959
HpyCH4III	ACNGT	1 cut(s)	123
HpyCH4IV	ACGT	3 cut(s)	480, 667, 729
HpyCH4V	TGCA	5 cut(s)	710, 716, 733, 865, 1030
HpyF10VI	GCNNNNNNNGC	2 cut(s)	243, 575
HpyF3I	CTNAG	3 cut(s)	87, 497, 1083
HpySE526I	ACGT	3 cut(s)	480, 667, 729
Hsp92II	CATG	7 cut(s)	188, 246, 281, 320, 720, 727, 862
KpnI	GGTACC	1 cut(s)	396
KspAI	GTTAAC	1 cut(s)	820
Kzo9I	GATC	3 cut(s)	196, 214, 619
LmnI	GCTCC	3 cut(s)	313, 698, 981
Lsp1109I	GCAGC	2 cut(s)	58, 1042
LweI	GCATC	2 cut(s)	719, 852
MaeI	CTAG	5 cut(s)	488, 545, 566, 570, 575
MaeII	ACGT	3 cut(s)	480, 667, 729
MalI	GATC	3 cut(s)	198, 216, 621
MboI	GATC	3 cut(s)	196, 214, 619
MboII	GAAGA	2 cut(s)	77, 560
MflI	RGATCY	3 cut(s)	196, 214, 619
MhlI	GDGCHC	3 cut(s)	735, 753, 986
MluCI	AATT	8 cut(s)	6, 24, 36, 258, 507, 522, 649, 825
MlyI	GAGTC	3 cut(s)	356, 468, 777
MmeI	TCCRAC	2 cut(s)	314, 720
MseI	TTAA	2 cut(s)	39, 819
MslI	CAYNNNNRTG	2 cut(s)	715, 728
MspI	CCGG	2 cut(s)	179, 959
MspR9I	CCNGG	3 cut(s)	149, 179, 960
MvaI	CCWGG	1 cut(s)	149
MwoI	GCNNNNNNNGC	2 cut(s)	243, 575
NciI	CCSGG	2 cut(s)	179, 960
NdeII	GATC	3 cut(s)	196, 214, 619
NheI	GCTAGC	1 cut(s)	574
NlaIII	CATG	7 cut(s)	188, 246, 281, 320, 720, 727, 862
NlaIV	GGNNCC	4 cut(s)	198, 394, 621, 1076
NspI	RCATGY	2 cut(s)	246, 320
PaeI	GCATGC	2 cut(s)	246, 320
PagI	TCATGA	1 cut(s)	184
PcsI	WCGNNNNNNNCGW	2 cut(s)	197, 783
PfeI	GAWTC	6 cut(s)	107, 140, 188, 202, 629, 976
PfoI	TCCNGGA	1 cut(s)	958
PkrI	GCNGC	3 cut(s)	48, 421, 1032
PleI	GAGTC	3 cut(s)	355, 468, 777
PpsI	GAGTC	3 cut(s)	355, 468, 777
Ppu21I	YACGTR	1 cut(s)	668
PshBI	ATTAAT	1 cut(s)	39
Psp124BI	GAGCTC	1 cut(s)	986
Psp6I	CCWGG	1 cut(s)	147
PspGI	CCWGG	1 cut(s)	147
PspN4I	GGNNCC	4 cut(s)	198, 394, 621, 1076
PstNI	CAGNNNCTG	1 cut(s)	226
PsuI	RGATCY	3 cut(s)	196, 214, 619
RsaI	GTAC	4 cut(s)	394, 445, 666, 887
RsaNI	GTAC	4 cut(s)	393, 444, 665, 886
RseI	CAYNNNNRTG	2 cut(s)	715, 728
SacI	GAGCTC	1 cut(s)	986
SaqAI	TTAA	2 cut(s)	39, 819
SatI	GCNGC	3 cut(s)	47, 420, 1031
Sau3AI	GATC	3 cut(s)	196, 214, 619
SchI	GAGTC	3 cut(s)	356, 468, 777
ScrFI	CCNGG	3 cut(s)	149, 179, 960
SduI	GDGCHC	3 cut(s)	735, 753, 986
SfaNI	GCATC	2 cut(s)	719, 852
SmiMI	CAYNNNNRTG	2 cut(s)	715, 728
SmlI	CTYRAG	1 cut(s)	1067
SmoI	CTYRAG	1 cut(s)	1067
SphI	GCATGC	2 cut(s)	246, 320
Sse9I	AATT	8 cut(s)	6, 24, 36, 258, 507, 522, 649, 825
SsiI	CCGC	1 cut(s)	419
SspMI	CTAG	5 cut(s)	488, 545, 566, 570, 575
SstI	GAGCTC	1 cut(s)	986
StyD4I	CCNGG	3 cut(s)	147, 177, 958
StyI	CCWWGG	1 cut(s)	610
TaaI	ACNGT	1 cut(s)	123
TaiI	ACGT	3 cut(s)	483, 670, 732
TaqI	TCGA	2 cut(s)	755, 786
TasI	AATT	8 cut(s)	6, 24, 36, 258, 507, 522, 649, 825
TatI	WGTACW	1 cut(s)	885
TauI	GCSGC	1 cut(s)	422
TfiI	GAWTC	6 cut(s)	107, 140, 188, 202, 629, 976
Tru1I	TTAA	2 cut(s)	39, 819
Tru9I	TTAA	2 cut(s)	39, 819
TscAI	CASTG	4 cut(s)	742, 820, 843, 976
TseI	GCWGC	2 cut(s)	46, 1030
TspDTI	ATGAA	2 cut(s)	173, 786
TspGWI	ACGGA	1 cut(s)	68
TspRI	CASTG	4 cut(s)	742, 820, 843, 976
VneI	GTGCAC	1 cut(s)	731
VspI	ATTAAT	1 cut(s)	39
XapI	RAATTY	2 cut(s)	258, 649
XceI	RCATGY	2 cut(s)	246, 320
XspI	CTAG	5 cut(s)	488, 545, 566, 570, 575

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.