RLG00000036117

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012155
Physical Location & Seq
Forward (+)
75943069 .. 75947078
4010 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036117

Sequence Viewer

Length: 1191 bp
ATGAACTTCGAATCTTACCCTCCTCCATTTCGCAACCTACTCCAACCGCCACTGAGCAGCCCGGCCCAGATCCTCGATTCTGGTGCCCTTAACTCGGATCCTATACCCGTCGTGGATCTCCAGTGCCTTGAGCTTGGGGACTGCAAGCTTCTTGAGGAGCCTTGTAAGCAATGGGGACTTTTTCGTTTGGTCAACCACGGAGTTCCTGAAAGTCTTTTGAGTCAAGTACAGGACTATGCCAGAACTCTTTTTTCTCTTACCTTTGAATCCAAACAGGCCCTTATTATTAGCCCTCTGTCTTACTTCTGGGGTACCCCTGCCCTTACTCCAACTGGGGCGGCGGTATCGGCCGAAAAACTCCAGAATATCAATTTGGTCGAAGGTCTCAACGTCCCTCTGAGTCAACTGCCTCGATTTCAAACTGACGATCCTAGAATTGCTTCTTTCTGCCATGTGCTAGAAGAATATGGGAGGCATCTAGCTAGAATTGGCAGAACCCTATTTAAAGGTATGTCCAGGAGCCTGAGTTTGGATCCAATAGAATACGAGTCAAATTTATCAGAATCTACTGGTTTAGTACGTGTATACCGATACCCACATTGGCCTTCGAAATATAAGAAAGAAAACAAGTCTGCATGGGGAATGGACGTGCACACTGACAGTTCCGTGCTGTCCATACTGAACCAGGACGAGGTCGGTGGCCTTGAAGTGCTCAAAGACAATCAGTGGTTCACTGTGAAACCCATTCGCAACACACTGGTCGTCAATCTTGGAGATATGATGCAGGCGATAAGCGATGATGAATACACAAGTGTGAAACACAGGGTAAAGGTGAACGAATACAGAGAGCGCATTTCAATTTGCTACTTTGTGTTTCCTGGCGAAAACAGTGTGATTCGAAGCTCAAACTACAAGCCTTTTACTTACAGGGATTTTCAAGAACAAGTGCAGAAAGACATAAAGACTGTTGGCTATAAGACTCTGAGGAGCTTGAAGGCGTTCTCCACCGACATCTCGAAGGGAACCGAGTCGTCTGCTCTGGAGCTAGCTTGGACCGCCGAAGCTAATAGAGCTGCCTTCCTCCTCGACCTCGACGAAACTTCGGTCGTCACAACCCTAACCCTCCTCTGCTGGTCTGAGAGTTGCCACCGTTTGTATGGCCGATGGAGATCGGAGGATTGTTTCTGTTGA

Protein Analysis

397

Amino Acids

44.96

Weight (kDa)

5.63

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 35 - 102 8.7e-12 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 196 - 293 4.5e-26 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58660 AT5G58660
fragaria_vesca FvH4_3g38920
malus_domestica MD03G1066600.v1.1 MD11G1071600.v1.1
prunus_persica Prupe.6G055300_v2.0.a1
pyrus_communis pycom03g05320 pycom11g05970
rosa_chinensis RchiOBHm_Chr5g0070171
rosa_laevigata RLG00000036117
rosa_multiflora Rmu_sc0013014.1_g000007 Rmu_sc0018909.1_g000006
rosa_roxburghii Rroxscaffold_1G00010950
rosa_rugosa Rorug05G0403600
rosa_samantha Rh5AG459500 Rh5BG478000 Rh5CG501900 Rh5DG489900
rosa_wichuraiana Rw5G042860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 311
AccB1I GGYRCC 2 cut(s) 83, 311
AccI GTMKAC 1 cut(s) 585
AciI CCGC 4 cut(s) 47, 338, 341, 1056
AclWI GGATC 7 cut(s) 64, 92, 105, 123, 422, 527, 540
AcoI YGGCCR 2 cut(s) 348, 1159
AcsI RAATTY 1 cut(s) 553
AfaI GTAC 3 cut(s) 228, 313, 579
AfiI CCNNNNNNNGG 3 cut(s) 94, 529, 691
AflIII ACRYGT 1 cut(s) 580
AgsI TTSAA 6 cut(s) 266, 419, 707, 858, 938, 994
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 3 cut(s) 515, 684, 877
AjuI GAANNNNNNNTTGG 2 cut(s) 356, 388
AleI CACNNNNGTG 1 cut(s) 812
AluBI AGCT 9 cut(s) 133, 148, 482, 903, 990, 1045, 1049, 1064, 1073
AluI AGCT 9 cut(s) 133, 148, 482, 903, 990, 1045, 1049, 1064, 1073
Alw21I GWGCWC 2 cut(s) 654, 714
Alw26I GTCTC 1 cut(s) 389
Alw44I GTGCAC 1 cut(s) 650
AlwI GGATC 7 cut(s) 64, 92, 105, 123, 422, 527, 540
AoxI GGCC 6 cut(s) 63, 276, 348, 602, 700, 1159
ApaLI GTGCAC 1 cut(s) 650
ApeKI GCWGC 2 cut(s) 57, 1073
ApoI RAATTY 1 cut(s) 553
ArsI GACNNNNNNTTYG 2 cut(s) 1089, 1121
Asp700I GAANNNNTTC 2 cut(s) 439, 998
Asp718I GGTACC 1 cut(s) 311
AspLEI GCGC 1 cut(s) 852
AspS9I GGNCC 3 cut(s) 64, 277, 1053
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 844
AsuII TTCGAA 3 cut(s) 9, 608, 898
AsuNHI GCTAGC 1 cut(s) 1045
AvaII GGWCC 1 cut(s) 1053
BaeGI GKGCMC 2 cut(s) 88, 654
BamHI GGATCC 2 cut(s) 97, 532
BanI GGYRCC 2 cut(s) 83, 311
Bbv12I GWGCWC 2 cut(s) 654, 714
BbvI GCAGC 2 cut(s) 69, 1060
BccI CCATC 1 cut(s) 1158
BcgI CGANNNNNNTGC 4 cut(s) 65, 99, 1016, 1050
BciT130I CCWGG 3 cut(s) 517, 686, 879
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 5 cut(s) 432, 458, 479, 483, 1046
BisI GCNGC 3 cut(s) 58, 339, 1074
BlsI GCNGC 3 cut(s) 59, 340, 1075
Bme1390I CCNGG 4 cut(s) 62, 517, 686, 879
Bme18I GGWCC 1 cut(s) 1053
BmgBI CACGTC 1 cut(s) 649
BmgT120I GGNCC 3 cut(s) 64, 277, 1053
BmiI GGNNCC 7 cut(s) 85, 99, 159, 313, 521, 534, 1024
BmrFI CCNGG 4 cut(s) 62, 517, 686, 879
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 2 cut(s) 484, 771
BmtI GCTAGC 1 cut(s) 1049
BmuI ACTGGG 1 cut(s) 342
BpmI CTGGAG 3 cut(s) 104, 344, 1061
Bpu14I TTCGAA 3 cut(s) 9, 608, 898
BpuEI CTTGAG 2 cut(s) 149, 173
BpuMI CCSGG 1 cut(s) 62
BsaAI YACGTR 1 cut(s) 581
BsaBI GATNNNNATC 1 cut(s) 1168
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 511, 541
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 529, 691
Bse1I ACTGG 4 cut(s) 121, 337, 574, 762
Bse3DI GCAATG 1 cut(s) 176
Bse8I GATNNNNATC 1 cut(s) 1168
BseBI CCWGG 3 cut(s) 517, 686, 879
BseDI CCNNGG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 1168
BseLI CCNNNNNNNGG 3 cut(s) 94, 529, 691
BseMI GCAATG 1 cut(s) 176
BseMII CTCAG 5 cut(s) 44, 389, 515, 974, 1128
BseNI ACTGG 4 cut(s) 121, 337, 574, 762
BseRI GAGGAG 5 cut(s) 12, 170, 1000, 1073, 1115
BseSI GKGCMC 2 cut(s) 88, 654
BseX3I CGGCCG 1 cut(s) 348
BseXI GCAGC 2 cut(s) 69, 1060
BsgI GTGCAG 1 cut(s) 968
Bsh1285I CGRYCG 2 cut(s) 351, 1107
BshFI GGCC 6 cut(s) 65, 278, 350, 604, 702, 1161
BshNI GGYRCC 2 cut(s) 83, 311
BsiEI CGRYCG 2 cut(s) 351, 1107
BsiHKAI GWGCWC 2 cut(s) 654, 714
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 3 cut(s) 152, 189, 377
BslI CCNNNNNNNGG 3 cut(s) 94, 529, 691
BsmAI GTCTC 1 cut(s) 389
BsmFI GGGAC 3 cut(s) 152, 189, 377
BsnI GGCC 6 cut(s) 65, 278, 350, 604, 702, 1161
Bso31I GGTCTC 1 cut(s) 389
Bsp119I TTCGAA 3 cut(s) 9, 608, 898
Bsp1286I GDGCHC 3 cut(s) 88, 654, 714
Bsp143I GATC 6 cut(s) 69, 97, 115, 427, 532, 1169
BspACI CCGC 4 cut(s) 47, 338, 341, 1056
BspANI GGCC 6 cut(s) 65, 278, 350, 604, 702, 1161
BspCNI CTCAG 5 cut(s) 45, 390, 516, 975, 1129
BspLI GGNNCC 7 cut(s) 85, 99, 159, 313, 521, 534, 1024
BspOI GCTAGC 1 cut(s) 1049
BspPI GGATC 7 cut(s) 64, 92, 105, 123, 422, 527, 540
BspT104I TTCGAA 3 cut(s) 9, 608, 898
BspT107I GGYRCC 2 cut(s) 83, 311
BspTNI GGTCTC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 176
BsrI ACTGG 4 cut(s) 121, 337, 574, 762
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 6 cut(s) 69, 97, 115, 427, 532, 1169
BssNAI GTATAC 1 cut(s) 586
Bst1107I GTATAC 1 cut(s) 586
Bst2UI CCWGG 3 cut(s) 517, 686, 879
Bst4CI ACNGT 5 cut(s) 662, 736, 890, 967, 1151
BstBAI YACGTR 1 cut(s) 581
BstBI TTCGAA 3 cut(s) 9, 608, 898
BstC8I GCNNGC 3 cut(s) 146, 786, 1047
BstDEI CTNAG 5 cut(s) 53, 398, 524, 983, 1137
BstDSI CCRYGG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 852
BstKTI GATC 6 cut(s) 72, 100, 118, 430, 535, 1172
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 6 cut(s) 69, 97, 115, 427, 532, 1169
BstMCI CGRYCG 2 cut(s) 351, 1107
BstMWI GCNNNNNNNGC 4 cut(s) 166, 347, 1055, 1070
BstNI CCWGG 3 cut(s) 517, 686, 879
BstSCI CCNGG 4 cut(s) 60, 515, 684, 877
BstSLI GKGCMC 2 cut(s) 88, 654
BstV1I GCAGC 2 cut(s) 69, 1060
BstX2I RGATCY 4 cut(s) 69, 97, 115, 532
BstYI RGATCY 4 cut(s) 69, 97, 115, 532
BstZ17I GTATAC 1 cut(s) 586
BstZI CGGCCG 1 cut(s) 348
BsuRI GGCC 6 cut(s) 65, 278, 350, 604, 702, 1161
BtgI CCRYGG 1 cut(s) 196
BtgZI GCGATG 1 cut(s) 810
BtrI CACGTC 1 cut(s) 649
BtsIMutI CAGTG 7 cut(s) 50, 128, 654, 731, 732, 755, 895
Cac8I GCNNGC 3 cut(s) 146, 786, 1047
CfoI GCGC 1 cut(s) 852
Cfr13I GGNCC 3 cut(s) 64, 277, 1053
Csp6I GTAC 3 cut(s) 227, 312, 578
CviAII CATG 2 cut(s) 452, 636
CviQI GTAC 3 cut(s) 227, 312, 578
DdeI CTNAG 5 cut(s) 53, 398, 524, 983, 1137
DpnI GATC 6 cut(s) 71, 99, 117, 429, 534, 1171
DpnII GATC 6 cut(s) 69, 97, 115, 427, 532, 1169
DraI TTTAAA 1 cut(s) 505
EaeI YGGCCR 2 cut(s) 348, 1159
EagI CGGCCG 1 cut(s) 348
EclXI CGGCCG 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 389
Eco47I GGWCC 1 cut(s) 1053
Eco52I CGGCCG 1 cut(s) 348
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 3 cut(s) 515, 684, 877
FaeI CATG 2 cut(s) 455, 639
FaqI GGGAC 3 cut(s) 152, 189, 377
FatI CATG 2 cut(s) 451, 635
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 3 cut(s) 58, 339, 1074
Fsp4HI GCNGC 3 cut(s) 58, 339, 1074
FspBI CTAG 5 cut(s) 432, 458, 479, 483, 1046
GlaI GCGC 1 cut(s) 851
GluI GCNGC 3 cut(s) 58, 339, 1074
GsuI CTGGAG 3 cut(s) 104, 344, 1061
HaeIII GGCC 6 cut(s) 65, 278, 350, 604, 702, 1161
HapII CCGG 1 cut(s) 62
HhaI GCGC 1 cut(s) 852
Hin1II CATG 2 cut(s) 455, 639
Hin6I GCGC 1 cut(s) 850
HinP1I GCGC 1 cut(s) 850
HincII GTYRAC 2 cut(s) 193, 404
HindII GTYRAC 2 cut(s) 193, 404
HindIII AAGCTT 1 cut(s) 146
HpaII CCGG 1 cut(s) 62
HphI GGTGA 1 cut(s) 844
Hpy166II GTNNAC 6 cut(s) 193, 404, 586, 652, 732, 835
Hpy188I TCNGA 6 cut(s) 97, 399, 562, 984, 1138, 1174
Hpy188III TCNNGA 6 cut(s) 152, 206, 361, 938, 1015, 1040
Hpy8I GTNNAC 6 cut(s) 193, 404, 586, 652, 732, 835
Hpy99I CGWCG 2 cut(s) 113, 1097
HpyAV CCTTC 5 cut(s) 374, 615, 988, 1012, 1087
HpyCH4III ACNGT 5 cut(s) 662, 736, 890, 967, 1151
HpyCH4IV ACGT 3 cut(s) 390, 580, 648
HpyCH4V TGCA 5 cut(s) 144, 635, 652, 784, 949
HpyF10VI GCNNNNNNNGC 4 cut(s) 166, 347, 1055, 1070
HpyF3I CTNAG 5 cut(s) 53, 398, 524, 983, 1137
HpySE526I ACGT 3 cut(s) 390, 580, 648
Hsp92II CATG 2 cut(s) 455, 639
HspAI GCGC 1 cut(s) 850
KpnI GGTACC 1 cut(s) 315
Kzo9I GATC 6 cut(s) 69, 97, 115, 427, 532, 1169
LmnI GCTCC 4 cut(s) 157, 519, 987, 1042
Lsp1109I GCAGC 2 cut(s) 69, 1060
LweI GCATC 2 cut(s) 484, 771
MaeI CTAG 5 cut(s) 432, 458, 479, 483, 1046
MaeII ACGT 3 cut(s) 390, 580, 648
MaeIII GTNAC 1 cut(s) 1108
MalI GATC 6 cut(s) 71, 99, 117, 429, 534, 1171
MboI GATC 6 cut(s) 69, 97, 115, 427, 532, 1169
MboII GAAGA 1 cut(s) 473
MflI RGATCY 4 cut(s) 69, 97, 115, 532
MhlI GDGCHC 3 cut(s) 88, 654, 714
MluCI AATT 5 cut(s) 370, 435, 486, 553, 858
MlyI GAGTC 5 cut(s) 229, 409, 557, 973, 1037
MmeI TCCRAC 2 cut(s) 67, 353
MroXI GAANNNNTTC 2 cut(s) 439, 998
MseI TTAA 2 cut(s) 90, 504
MslI CAYNNNNRTG 1 cut(s) 812
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 4 cut(s) 62, 517, 686, 879
MvaI CCWGG 3 cut(s) 517, 686, 879
MwoI GCNNNNNNNGC 4 cut(s) 166, 347, 1055, 1070
NciI CCSGG 1 cut(s) 62
NdeII GATC 6 cut(s) 69, 97, 115, 427, 532, 1169
NheI GCTAGC 1 cut(s) 1045
NlaIII CATG 2 cut(s) 455, 639
NlaIV GGNNCC 7 cut(s) 85, 99, 159, 313, 521, 534, 1024
NmuCI GTSAC 1 cut(s) 1108
NspV TTCGAA 3 cut(s) 9, 608, 898
OliI CACNNNNGTG 1 cut(s) 812
PcsI WCGNNNNNNNCGW 2 cut(s) 586, 1092
PdmI GAANNNNTTC 2 cut(s) 439, 998
PfeI GAWTC 5 cut(s) 11, 77, 266, 563, 895
PflFI GACNNNGTC 1 cut(s) 692
PfoI TCCNGGA 1 cut(s) 515
PkrI GCNGC 3 cut(s) 59, 340, 1075
PleI GAGTC 5 cut(s) 228, 408, 556, 973, 1036
PpsI GAGTC 5 cut(s) 228, 408, 556, 973, 1036
Ppu21I YACGTR 1 cut(s) 581
Psp6I CCWGG 3 cut(s) 515, 684, 877
PspGI CCWGG 3 cut(s) 515, 684, 877
PspN4I GGNNCC 7 cut(s) 85, 99, 159, 313, 521, 534, 1024
PspPI GGNCC 3 cut(s) 64, 277, 1053
PsuI RGATCY 4 cut(s) 69, 97, 115, 532
PsyI GACNNNGTC 1 cut(s) 692
RsaI GTAC 3 cut(s) 228, 313, 579
RsaNI GTAC 3 cut(s) 227, 312, 578
RseI CAYNNNNRTG 1 cut(s) 812
SaqAI TTAA 2 cut(s) 90, 504
SatI GCNGC 3 cut(s) 58, 339, 1074
Sau3AI GATC 6 cut(s) 69, 97, 115, 427, 532, 1169
Sau96I GGNCC 3 cut(s) 64, 277, 1053
SchI GAGTC 5 cut(s) 229, 409, 557, 973, 1037
ScrFI CCNGG 4 cut(s) 62, 517, 686, 879
SduI GDGCHC 3 cut(s) 88, 654, 714
SfaNI GCATC 2 cut(s) 484, 771
SfuI TTCGAA 3 cut(s) 9, 608, 898
SinI GGWCC 1 cut(s) 1053
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 2 cut(s) 128, 152
SmoI CTYRAG 2 cut(s) 128, 152
Sse9I AATT 5 cut(s) 370, 435, 486, 553, 858
SsiI CCGC 4 cut(s) 47, 338, 341, 1056
SspMI CTAG 5 cut(s) 432, 458, 479, 483, 1046
StyD4I CCNGG 4 cut(s) 60, 515, 684, 877
TaaI ACNGT 5 cut(s) 662, 736, 890, 967, 1151
TaiI ACGT 3 cut(s) 393, 583, 651
TaqI TCGA 9 cut(s) 9, 75, 378, 412, 608, 898, 1016, 1086, 1092
TaqII GACCGA 1 cut(s) 1093
TasI AATT 5 cut(s) 370, 435, 486, 553, 858
TatI WGTACW 1 cut(s) 226
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 5 cut(s) 11, 77, 266, 563, 895
Tru1I TTAA 2 cut(s) 90, 504
Tru9I TTAA 2 cut(s) 90, 504
TscAI CASTG 7 cut(s) 57, 128, 661, 731, 739, 762, 895
TseFI GTSAC 1 cut(s) 1108
TseI GCWGC 2 cut(s) 57, 1073
Tsp45I GTSAC 1 cut(s) 1108
TspDTI ATGAA 2 cut(s) 17, 816
TspGWI ACGGA 2 cut(s) 213, 655
TspRI CASTG 7 cut(s) 57, 128, 661, 731, 739, 762, 895
Tth111I GACNNNGTC 1 cut(s) 692
VneI GTGCAC 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 1053
XapI RAATTY 1 cut(s) 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 1154
XmiI GTMKAC 1 cut(s) 585
XmnI GAANNNNTTC 2 cut(s) 439, 998
XspI CTAG 5 cut(s) 432, 458, 479, 483, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.