Rh5CG501900

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012155
Physical Location & Seq
Forward (+)
69744865 .. 69747231
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG501900.1

Sequence Viewer

Length: 1023 bp
ATGAGCTTCGAATCTTACCCTCCTCCATTTCGCAACCTACTCCAACCGCCACTGAGCAGCCCGGCCCAGATCCTCGATTCGGGATGGGGTGCCCTTAACTCGGATCCTATACCCGTCGTGGATCTTCAGTGCCTTGAGCTTGGGGACTGCAAGCTTCTTGAGGAGCCTTGTAAGCAATGGGGACTTTTTCGTTTGGTCAACCACGGAGTTCCTGAAAGTCTTTTGAGTCAAGCACAGGACTATGCCAGAACGCTTTTTTCTCTTACCTTTGAATCCAAACAGGCCCTTATTACTAGCCCTCTGTCTTACTTCTGGGGTACCCCTGCCCTTACTCCAACTGGGGCGGCGTTATCGGCCGAAAAACTCCAGAATATCAATTTGGTCGAAGGTCTCAACGTCCCTCTGAGTCAACTGCCTCGATTTCAAACTGACGATCCTAGAATTGCTTCTTTCTGCCATGTGCTAGAAGAATATGGGAGGCATCTAGCTAGAATTGGCAGAACCCTATTTAAAGGTATGTCCACGAGCCTGAGTTTGGATCCAATTGAATACGAGTCAAATTTATCAGAATCTACTGGTTTAGTACGTGTATACCGATACCCACATTGGCCTTCGAAATATAAGAAAGAAAACAAGTCTGCATGGGGAATGGACGTGCACACTGACAGTTCCGTGCTGTCCATACTGAACCAGGACGAGGTCGGTGGCCTTGAAGTGCTCAAAGACAATCAGTGGTTCACTGTGAAACCCATTCCCAACACACTGGTCGTCAATCTTGGAGATATGATGCAGGCGATAAGCGATGATGAATACACAAGTGTGAAACACAGGGTAAAGGTGAACGAATACAGAGAGCGCATTTCAATTTGCTACTTTGTGTTTCCTGGCGAAAACAGTGTGATTCGAAGCTCAAACTACAAGCCTTTTACTTACAGGGATTTTCAAGAACAAGTGCAGAAAGACATAAAGACTGTTGGCTATAAGGTTGGCCTTAACAGATTCAAGCTTAGAGATGCTTGTTAA

Protein Analysis

340

Amino Acids

38.55

Weight (kDa)

5.93

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 37 - 104 8.1e-12 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 198 - 295 9.9e-27 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58660 AT5G58660
fragaria_vesca FvH4_3g38920
malus_domestica MD03G1066600.v1.1 MD11G1071600.v1.1
prunus_persica Prupe.6G055300_v2.0.a1
pyrus_communis pycom03g05320 pycom11g05970
rosa_chinensis RchiOBHm_Chr5g0070171
rosa_laevigata RLG00000036117
rosa_multiflora Rmu_sc0013014.1_g000007 Rmu_sc0018909.1_g000006
rosa_roxburghii Rroxscaffold_1G00010950
rosa_rugosa Rorug05G0403600
rosa_samantha Rh5AG459500 Rh5BG478000 Rh5CG501900 Rh5DG489900
rosa_wichuraiana Rw5G042860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 2 cut(s) 89, 317
AccI GTMKAC 1 cut(s) 591
AciI CCGC 2 cut(s) 47, 344
AclWI GGATC 7 cut(s) 64, 98, 111, 129, 428, 533, 546
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 1 cut(s) 559
AcuI CTGAAG 1 cut(s) 110
AfaI GTAC 2 cut(s) 319, 585
AfiI CCNNNNNNNGG 4 cut(s) 79, 100, 535, 697
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 7 cut(s) 272, 425, 548, 713, 864, 944, 1003
AjiI CACGTC 1 cut(s) 655
AjnI CCWGG 2 cut(s) 690, 883
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AleI CACNNNNGTG 1 cut(s) 818
AluBI AGCT 6 cut(s) 6, 139, 154, 488, 909, 1006
AluI AGCT 6 cut(s) 6, 139, 154, 488, 909, 1006
Alw21I GWGCWC 2 cut(s) 660, 720
Alw26I GTCTC 1 cut(s) 395
Alw44I GTGCAC 1 cut(s) 656
AlwI GGATC 7 cut(s) 64, 98, 111, 129, 428, 533, 546
AoxI GGCC 6 cut(s) 63, 282, 354, 608, 706, 988
ApaLI GTGCAC 1 cut(s) 656
ApeKI GCWGC 1 cut(s) 57
ApoI RAATTY 1 cut(s) 559
Asp700I GAANNNNTTC 1 cut(s) 445
Asp718I GGTACC 1 cut(s) 317
AspLEI GCGC 1 cut(s) 858
AspS9I GGNCC 2 cut(s) 64, 283
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 850
AsuII TTCGAA 3 cut(s) 9, 614, 904
BaeGI GKGCMC 2 cut(s) 94, 660
BamHI GGATCC 2 cut(s) 103, 538
BanI GGYRCC 2 cut(s) 89, 317
BauI CACGAG 1 cut(s) 523
Bbv12I GWGCWC 2 cut(s) 660, 720
BbvI GCAGC 1 cut(s) 69
BccI CCATC 1 cut(s) 78
BciT130I CCWGG 2 cut(s) 692, 885
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 5 cut(s) 294, 438, 464, 485, 489
BisI GCNGC 2 cut(s) 58, 345
BlsI GCNGC 2 cut(s) 59, 346
Bme1390I CCNGG 3 cut(s) 62, 692, 885
BmgBI CACGTC 1 cut(s) 655
BmgT120I GGNCC 2 cut(s) 64, 283
BmiI GGNNCC 5 cut(s) 91, 105, 165, 319, 540
BmrFI CCNGG 3 cut(s) 62, 692, 885
BmrI ACTGGG 1 cut(s) 348
BmsI GCATC 3 cut(s) 490, 777, 1003
BmuI ACTGGG 1 cut(s) 348
BpmI CTGGAG 1 cut(s) 350
Bpu14I TTCGAA 3 cut(s) 9, 614, 904
BpuEI CTTGAG 2 cut(s) 155, 179
BpuMI CCSGG 1 cut(s) 62
BsaAI YACGTR 1 cut(s) 587
BsaI GGTCTC 1 cut(s) 395
BsaJI CCNNGG 1 cut(s) 202
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 100, 535, 697
Bse1I ACTGG 3 cut(s) 343, 580, 768
Bse3DI GCAATG 1 cut(s) 182
BseBI CCWGG 2 cut(s) 692, 885
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 1 cut(s) 89
BseLI CCNNNNNNNGG 4 cut(s) 79, 100, 535, 697
BseMI GCAATG 1 cut(s) 182
BseMII CTCAG 3 cut(s) 44, 395, 521
BseNI ACTGG 3 cut(s) 343, 580, 768
BseRI GAGGAG 2 cut(s) 12, 176
BseSI GKGCMC 2 cut(s) 94, 660
BseX3I CGGCCG 1 cut(s) 354
BseXI GCAGC 1 cut(s) 69
BsgI GTGCAG 1 cut(s) 974
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 6 cut(s) 65, 284, 356, 610, 708, 990
BshNI GGYRCC 2 cut(s) 89, 317
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 2 cut(s) 660, 720
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 3 cut(s) 158, 195, 383
BslI CCNNNNNNNGG 4 cut(s) 79, 100, 535, 697
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 3 cut(s) 158, 195, 383
BsnI GGCC 6 cut(s) 65, 284, 356, 610, 708, 990
Bso31I GGTCTC 1 cut(s) 395
Bsp119I TTCGAA 3 cut(s) 9, 614, 904
Bsp1286I GDGCHC 3 cut(s) 94, 660, 720
Bsp143I GATC 5 cut(s) 69, 103, 121, 433, 538
BspACI CCGC 2 cut(s) 47, 344
BspANI GGCC 6 cut(s) 65, 284, 356, 610, 708, 990
BspCNI CTCAG 3 cut(s) 45, 396, 522
BspLI GGNNCC 5 cut(s) 91, 105, 165, 319, 540
BspPI GGATC 7 cut(s) 64, 98, 111, 129, 428, 533, 546
BspT104I TTCGAA 3 cut(s) 9, 614, 904
BspT107I GGYRCC 2 cut(s) 89, 317
BspTNI GGTCTC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 182
BsrI ACTGG 3 cut(s) 343, 580, 768
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 5 cut(s) 69, 103, 121, 433, 538
BssNAI GTATAC 1 cut(s) 592
BssSI CACGAG 1 cut(s) 523
Bst1107I GTATAC 1 cut(s) 592
Bst2BI CACGAG 1 cut(s) 523
Bst2UI CCWGG 2 cut(s) 692, 885
Bst4CI ACNGT 4 cut(s) 668, 742, 896, 973
BstBAI YACGTR 1 cut(s) 587
BstBI TTCGAA 3 cut(s) 9, 614, 904
BstC8I GCNNGC 2 cut(s) 152, 792
BstDEI CTNAG 4 cut(s) 53, 404, 530, 1007
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 89
BstHHI GCGC 1 cut(s) 858
BstKTI GATC 5 cut(s) 72, 106, 124, 436, 541
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 5 cut(s) 69, 103, 121, 433, 538
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 2 cut(s) 172, 353
BstNI CCWGG 2 cut(s) 692, 885
BstSCI CCNGG 3 cut(s) 60, 690, 883
BstSLI GKGCMC 2 cut(s) 94, 660
BstV1I GCAGC 1 cut(s) 69
BstX2I RGATCY 4 cut(s) 69, 103, 121, 538
BstXI CCANNNNNNTGG 1 cut(s) 763
BstYI RGATCY 4 cut(s) 69, 103, 121, 538
BstZ17I GTATAC 1 cut(s) 592
BstZI CGGCCG 1 cut(s) 354
BsuRI GGCC 6 cut(s) 65, 284, 356, 610, 708, 990
BtgI CCRYGG 1 cut(s) 202
BtgZI GCGATG 1 cut(s) 816
BtrI CACGTC 1 cut(s) 655
BtsCI GGATG 1 cut(s) 89
BtsIMutI CAGTG 7 cut(s) 50, 134, 660, 737, 738, 761, 901
Cac8I GCNNGC 2 cut(s) 152, 792
CfoI GCGC 1 cut(s) 858
Cfr13I GGNCC 2 cut(s) 64, 283
Csp6I GTAC 2 cut(s) 318, 584
CviAII CATG 2 cut(s) 458, 642
CviQI GTAC 2 cut(s) 318, 584
DdeI CTNAG 4 cut(s) 53, 404, 530, 1007
DpnI GATC 5 cut(s) 71, 105, 123, 435, 540
DpnII GATC 5 cut(s) 69, 103, 121, 433, 538
DraI TTTAAA 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 354
EagI CGGCCG 1 cut(s) 354
EclXI CGGCCG 1 cut(s) 354
Eco31I GGTCTC 1 cut(s) 395
Eco52I CGGCCG 1 cut(s) 354
Eco57I CTGAAG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 690, 883
FaeI CATG 2 cut(s) 461, 645
FaqI GGGAC 3 cut(s) 158, 195, 383
FatI CATG 2 cut(s) 457, 641
FblI GTMKAC 1 cut(s) 591
Fnu4HI GCNGC 2 cut(s) 58, 345
FokI GGATG 1 cut(s) 96
Fsp4HI GCNGC 2 cut(s) 58, 345
FspBI CTAG 5 cut(s) 294, 438, 464, 485, 489
GlaI GCGC 1 cut(s) 857
GluI GCNGC 2 cut(s) 58, 345
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 6 cut(s) 65, 284, 356, 610, 708, 990
HapII CCGG 1 cut(s) 62
HhaI GCGC 1 cut(s) 858
Hin1II CATG 2 cut(s) 461, 645
Hin6I GCGC 1 cut(s) 856
HinP1I GCGC 1 cut(s) 856
HincII GTYRAC 2 cut(s) 199, 410
HindII GTYRAC 2 cut(s) 199, 410
HindIII AAGCTT 2 cut(s) 152, 1004
HinfI GANTC 9 cut(s) 11, 77, 226, 272, 406, 554, 569, 901, 999
HpaII CCGG 1 cut(s) 62
HphI GGTGA 1 cut(s) 850
Hpy166II GTNNAC 7 cut(s) 199, 410, 522, 592, 658, 738, 841
Hpy188I TCNGA 3 cut(s) 103, 405, 568
Hpy188III TCNNGA 5 cut(s) 81, 158, 212, 367, 944
Hpy8I GTNNAC 7 cut(s) 199, 410, 522, 592, 658, 738, 841
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 380, 621
HpyCH4III ACNGT 4 cut(s) 668, 742, 896, 973
HpyCH4IV ACGT 3 cut(s) 396, 586, 654
HpyCH4V TGCA 5 cut(s) 150, 641, 658, 790, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 172, 353
HpyF3I CTNAG 4 cut(s) 53, 404, 530, 1007
HpySE526I ACGT 3 cut(s) 396, 586, 654
Hsp92II CATG 2 cut(s) 461, 645
HspAI GCGC 1 cut(s) 856
KpnI GGTACC 1 cut(s) 321
Kzo9I GATC 5 cut(s) 69, 103, 121, 433, 538
LmnI GCTCC 1 cut(s) 163
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 3 cut(s) 490, 777, 1003
MaeI CTAG 5 cut(s) 294, 438, 464, 485, 489
MaeII ACGT 3 cut(s) 396, 586, 654
MalI GATC 5 cut(s) 71, 105, 123, 435, 540
MboI GATC 5 cut(s) 69, 103, 121, 433, 538
MboII GAAGA 2 cut(s) 116, 479
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 4 cut(s) 69, 103, 121, 538
MhlI GDGCHC 3 cut(s) 94, 660, 720
MluCI AATT 6 cut(s) 376, 441, 492, 543, 559, 864
MlyI GAGTC 3 cut(s) 235, 415, 563
MmeI TCCRAC 2 cut(s) 67, 359
MnlI CCTC 9 cut(s) 30, 33, 83, 154, 309, 411, 426, 471, 691
MroXI GAANNNNTTC 1 cut(s) 445
MseI TTAA 4 cut(s) 96, 510, 993, 1021
MslI CAYNNNNRTG 1 cut(s) 818
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 3 cut(s) 62, 692, 885
MunI CAATTG 1 cut(s) 543
MvaI CCWGG 2 cut(s) 692, 885
MwoI GCNNNNNNNGC 2 cut(s) 172, 353
NciI CCSGG 1 cut(s) 62
NdeII GATC 5 cut(s) 69, 103, 121, 433, 538
NlaIII CATG 2 cut(s) 461, 645
NlaIV GGNNCC 5 cut(s) 91, 105, 165, 319, 540
NspV TTCGAA 3 cut(s) 9, 614, 904
OliI CACNNNNGTG 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 445
PfeI GAWTC 6 cut(s) 11, 77, 272, 569, 901, 999
PflFI GACNNNGTC 1 cut(s) 698
PkrI GCNGC 2 cut(s) 59, 346
PleI GAGTC 3 cut(s) 234, 414, 562
PpsI GAGTC 3 cut(s) 234, 414, 562
Ppu21I YACGTR 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 690, 883
PspGI CCWGG 2 cut(s) 690, 883
PspN4I GGNNCC 5 cut(s) 91, 105, 165, 319, 540
PspPI GGNCC 2 cut(s) 64, 283
PsuI RGATCY 4 cut(s) 69, 103, 121, 538
PsyI GACNNNGTC 1 cut(s) 698
RsaI GTAC 2 cut(s) 319, 585
RsaNI GTAC 2 cut(s) 318, 584
RseI CAYNNNNRTG 1 cut(s) 818
SaqAI TTAA 4 cut(s) 96, 510, 993, 1021
SatI GCNGC 2 cut(s) 58, 345
Sau3AI GATC 5 cut(s) 69, 103, 121, 433, 538
Sau96I GGNCC 2 cut(s) 64, 283
SchI GAGTC 3 cut(s) 235, 415, 563
ScrFI CCNGG 3 cut(s) 62, 692, 885
SduI GDGCHC 3 cut(s) 94, 660, 720
SfaNI GCATC 3 cut(s) 490, 777, 1003
SfuI TTCGAA 3 cut(s) 9, 614, 904
SmiMI CAYNNNNRTG 1 cut(s) 818
SmlI CTYRAG 2 cut(s) 134, 158
SmoI CTYRAG 2 cut(s) 134, 158
Sse9I AATT 6 cut(s) 376, 441, 492, 543, 559, 864
SsiI CCGC 2 cut(s) 47, 344
SspMI CTAG 5 cut(s) 294, 438, 464, 485, 489
StyD4I CCNGG 3 cut(s) 60, 690, 883
TaaI ACNGT 4 cut(s) 668, 742, 896, 973
TaiI ACGT 3 cut(s) 399, 589, 657
TaqI TCGA 6 cut(s) 9, 75, 384, 418, 614, 904
TasI AATT 6 cut(s) 376, 441, 492, 543, 559, 864
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 6 cut(s) 11, 77, 272, 569, 901, 999
Tru1I TTAA 4 cut(s) 96, 510, 993, 1021
Tru9I TTAA 4 cut(s) 96, 510, 993, 1021
TscAI CASTG 7 cut(s) 57, 134, 667, 737, 745, 768, 901
TseI GCWGC 1 cut(s) 57
TspDTI ATGAA 1 cut(s) 822
TspGWI ACGGA 2 cut(s) 219, 661
TspRI CASTG 7 cut(s) 57, 134, 667, 737, 745, 768, 901
Tth111I GACNNNGTC 1 cut(s) 698
VneI GTGCAC 1 cut(s) 656
XapI RAATTY 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 591
XmnI GAANNNNTTC 1 cut(s) 445
XspI CTAG 5 cut(s) 294, 438, 464, 485, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.