MD03G1068300.v1.1

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009893
Physical Location & Seq
Forward (+)
5554430 .. 5556318
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1068300.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGAAGTTACTAATTTCTTCCCCTTCTTTTTCGTCATGCTCATCATCATCCACTACCACTGCTTTTGACGAAACAATGTGCAGTTCGAAAAGCGCCACCGCCGGCTGCATTGCCGGAATTTTGCGCAGAATTCTATGCTATGGTAGCTTCCCCACATACCCTTCTGACCACATCACAGAAGAAAAAGAAAATTCTGTGGAACCTAGTTCCAAGGATCAAGATTTCAATTCCAATGAGAAAACAGGGGAGAAAACAGAGATCTCTGCCCCTCCAAGCATTGTGGCAAGGCTGATGGGATTGGATTCAATTCCAGATGACAACTTGGTCAGCAGCCAATCAACCCAAAATTCAGTTTCACGCAGCAAATCGATGAATTCTGCAGACCGGTTCGATGGATGTGACTCAATGCAGCATCGCCGGGTAAAGTCGAGTCGGTCATTTAGAGAGATGCCTACGTTTCTTGAGCTAGATAATGAGGAATTCTTTGTTCTCAGCTTTGAGAGTGAGAGGAAAAGCAAGGAAACAGAAACAAAAGGGAGCAAATGTGAAAAGGAACAGAGCGAAAAAACCGAAAACAGAAGAAAAAGAAAGGCAGAGAAGAAGAAGAAGCAACTTGAAGGAGGACAAAGCAGAAGGACTTTGAAAGATTTGAATGGAAGAGAGATGCTGAGAAGCAGAAGCATTTCAGATAGAAGCACTTCTGATAAAGATTCAAAAAGTACTTCAATTGCTACAAAGATGGTAGGGAAGAAGACTATATGTGATGTGCTTACAAATGTAGAATCTGAGTTCAGCTCAGAAGATTTAAGCCCAGTTTCGATTCTCGATTGTGGTCAATTTCTTGTTGATCCGGAAGTCCTTTCTTCAGAAGAAGATTCAAGTTTAGCCAATTCATGCAAAGAAAATGGTCCAAATGGTGATGCAAGAAGAACAAAGACAAGAGAAAGCAAATGTCTTGGATCAAAGAAGAAAGAATTCCAGACAGCAAAGCACATTGGGATGTGGAGTGAGATTTGTAGGCTGACTGAAGCTGAATTGGTCGGTTCAAATTGGATCATAAAAAACAAGGGCATGAGGAATTTTGAAGGTATTTCTGCAGGCATTGGTGCAGATTTCGAGTCACAGATTCTTTGTCAATTATTAGACGAGCTTGTAGATCAATTTGCTGATTTTCCCATGGAAATTCAGAACGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VARLMGL

Protein Analysis

399

Amino Acids

44.28

Weight (kDa)

5.64

Isoelectric Point (pI)

61.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 87 - 107 1.1e-07 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58630
fragaria_vesca FvH4_3g38760 FvH4_3g38760
malus_domestica MD03G1068300.v1.1 MD11G1072700.v1.1
prunus_persica Prupe.6G056200_v2.0.a1
pyrus_communis pycom03g05470 pycom03g05480 pycom11g06040
rosa_chinensis RchiOBHm_Chr5g0069911
rosa_laevigata RLG00000036107
rosa_multiflora Rmu_ssc0000133.1_g000001
rosa_roxburghii Rroxscaffold_1G00011150
rosa_rugosa Rorug05G0400700
rosa_samantha Rh5AG457800 Rh5BG476600 Rh5BG476700 Rh5CG500400 Rh5CG500500 Rh5DG488500
rosa_wichuraiana Rw5G042750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 323
Acc16I TGCGCA 1 cut(s) 125
AccIII TCCGGA 1 cut(s) 850
AciI CCGC 1 cut(s) 99
AclWI GGATC 4 cut(s) 222, 842, 967, 1061
AcsI RAATTY 9 cut(s) 117, 129, 190, 346, 373, 479, 974, 1078, 1182
AcuI CTGAAG 2 cut(s) 849, 1047
AfaI GTAC 1 cut(s) 721
AflIII ACRYGT 1 cut(s) 1191
AgeI ACCGGT 1 cut(s) 384
AluBI AGCT 6 cut(s) 147, 466, 495, 795, 1031, 1150
AluI AGCT 6 cut(s) 147, 466, 495, 795, 1031, 1150
AlwI GGATC 4 cut(s) 222, 842, 967, 1061
Aor13HI TCCGGA 1 cut(s) 850
ApeKI GCWGC 4 cut(s) 105, 330, 360, 409
ApoI RAATTY 9 cut(s) 117, 129, 190, 346, 373, 479, 974, 1078, 1182
AsiGI ACCGGT 1 cut(s) 384
Asp700I GAANNNNTTC 3 cut(s) 682, 697, 974
AspLEI GCGC 2 cut(s) 95, 126
AspS9I GGNCC 1 cut(s) 908
AsuC2I CCSGG 1 cut(s) 419
AsuHPI GGTGA 1 cut(s) 929
AsuII TTCGAA 1 cut(s) 86
AvaII GGWCC 1 cut(s) 908
BbsI GAAGAC 1 cut(s) 758
BbvI GCAGC 4 cut(s) 92, 342, 372, 421
BccI CCATC 3 cut(s) 286, 386, 733
BcnI CCSGG 1 cut(s) 419
BfaI CTAG 2 cut(s) 204, 467
BfmI CTRYAG 2 cut(s) 378, 1095
BfoI RGCGCY 1 cut(s) 96
BglII AGATCT 1 cut(s) 258
BisI GCNGC 4 cut(s) 106, 331, 361, 410
BlsI GCNGC 4 cut(s) 107, 332, 362, 411
BmcAI AGTACT 1 cut(s) 721
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 908
BmgT120I GGNCC 1 cut(s) 908
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 419
BmrI ACTGGG 1 cut(s) 806
BmsI GCATC 4 cut(s) 421, 438, 654, 910
BmuI ACTGGG 1 cut(s) 806
BpiI GAAGAC 1 cut(s) 758
BplI GAGNNNNNCTC 2 cut(s) 779, 811
Bpu14I TTCGAA 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 419
Bsa29I ATCGAT 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 210, 1176
BsaWI WCCGGW 2 cut(s) 384, 850
BsaXI ACNNNNNCTCC 2 cut(s) 529, 559
Bse118I RCCGGY 2 cut(s) 101, 384
Bse1I ACTGG 1 cut(s) 812
Bse3DI GCAATG 1 cut(s) 108
BseAI TCCGGA 1 cut(s) 850
BseCI ATCGAT 1 cut(s) 368
BseDI CCNNGG 2 cut(s) 210, 1176
BseGI GGATG 3 cut(s) 47, 401, 1005
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 4 cut(s) 505, 659, 777, 810
BseNI ACTGG 1 cut(s) 812
BseXI GCAGC 4 cut(s) 92, 342, 372, 421
BsgI GTGCAG 2 cut(s) 100, 1128
BshTI ACCGGT 1 cut(s) 384
BshVI ATCGAT 1 cut(s) 368
BsiSI CCGG 5 cut(s) 102, 114, 385, 418, 851
Bsp119I TTCGAA 1 cut(s) 86
Bsp13I TCCGGA 1 cut(s) 850
Bsp143I GATC 6 cut(s) 214, 258, 847, 959, 1053, 1156
Bsp19I CCATGG 1 cut(s) 1176
BspACI CCGC 1 cut(s) 99
BspCNI CTCAG 4 cut(s) 504, 660, 778, 809
BspDI ATCGAT 1 cut(s) 368
BspEI TCCGGA 1 cut(s) 850
BspLI GGNNCC 1 cut(s) 201
BspMAI CTGCAG 2 cut(s) 382, 1099
BspPI GGATC 4 cut(s) 222, 842, 967, 1061
BspT104I TTCGAA 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 101, 384
BsrI ACTGG 1 cut(s) 812
BssAI RCCGGY 2 cut(s) 101, 384
BssECI CCNNGG 2 cut(s) 210, 1176
BssMI GATC 6 cut(s) 214, 258, 847, 959, 1053, 1156
BssT1I CCWWGG 2 cut(s) 210, 1176
Bst6I CTCTTC 1 cut(s) 652
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 2 cut(s) 103, 1099
BstDEI CTNAG 4 cut(s) 491, 668, 786, 796
BstDSI CCRYGG 1 cut(s) 1176
BstF5I GGATG 3 cut(s) 47, 401, 1005
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 126
BstKTI GATC 6 cut(s) 217, 261, 850, 962, 1056, 1159
BstMBI GATC 6 cut(s) 214, 258, 847, 959, 1053, 1156
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 417
BstSFI CTRYAG 2 cut(s) 378, 1095
BstV1I GCAGC 4 cut(s) 92, 342, 372, 421
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
Bsu15I ATCGAT 1 cut(s) 368
BsuTUI ATCGAT 1 cut(s) 368
BtgI CCRYGG 1 cut(s) 1176
BtgZI GCGATG 1 cut(s) 398
BtsCI GGATG 3 cut(s) 47, 401, 1005
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 103, 1099
CfoI GCGC 2 cut(s) 95, 126
Cfr10I RCCGGY 2 cut(s) 101, 384
Cfr13I GGNCC 1 cut(s) 908
ClaI ATCGAT 1 cut(s) 368
Csp6I GTAC 1 cut(s) 720
CspAI ACCGGT 1 cut(s) 384
CspCI CAANNNNNGTGG 4 cut(s) 46, 81, 261, 296
CviAII CATG 4 cut(s) 36, 894, 1072, 1177
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 4 cut(s) 491, 668, 786, 796
DpnI GATC 6 cut(s) 216, 260, 849, 961, 1055, 1158
DpnII GATC 6 cut(s) 214, 258, 847, 959, 1053, 1156
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
Eam1104I CTCTTC 1 cut(s) 652
EarI CTCTTC 1 cut(s) 652
Eco130I CCWWGG 2 cut(s) 210, 1176
Eco47I GGWCC 1 cut(s) 908
Eco57I CTGAAG 2 cut(s) 849, 1047
EcoRI GAATTC 4 cut(s) 129, 373, 479, 974
EcoT14I CCWWGG 2 cut(s) 210, 1176
ErhI CCWWGG 2 cut(s) 210, 1176
FaeI CATG 4 cut(s) 39, 897, 1075, 1180
FatI CATG 4 cut(s) 35, 893, 1071, 1176
Fnu4HI GCNGC 4 cut(s) 106, 331, 361, 410
FokI GGATG 3 cut(s) 34, 408, 1012
Fsp4HI GCNGC 4 cut(s) 106, 331, 361, 410
FspBI CTAG 2 cut(s) 204, 467
FspI TGCGCA 1 cut(s) 125
GlaI GCGC 2 cut(s) 94, 125
GluI GCNGC 4 cut(s) 106, 331, 361, 410
HaeII RGCGCY 1 cut(s) 96
HapII CCGG 5 cut(s) 102, 114, 385, 418, 851
HhaI GCGC 2 cut(s) 95, 126
Hin1II CATG 4 cut(s) 39, 897, 1075, 1180
Hin6I GCGC 2 cut(s) 93, 124
HinP1I GCGC 2 cut(s) 93, 124
HinfI GANTC 9 cut(s) 302, 401, 430, 710, 782, 820, 875, 1118, 1126
HpaII CCGG 5 cut(s) 102, 114, 385, 418, 851
HphI GGTGA 1 cut(s) 929
Hpy188I TCNGA 7 cut(s) 166, 688, 703, 787, 799, 868, 1188
Hpy188III TCNNGA 6 cut(s) 218, 311, 461, 824, 851, 979
HpyAV CCTTC 5 cut(s) 33, 171, 611, 627, 1079
HpyCH4IV ACGT 2 cut(s) 455, 1191
HpyCH4V TGCA 8 cut(s) 81, 108, 380, 409, 897, 923, 1097, 1109
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 4 cut(s) 491, 668, 786, 796
HpySE526I ACGT 2 cut(s) 455, 1191
Hsp92II CATG 4 cut(s) 39, 897, 1075, 1180
HspAI GCGC 2 cut(s) 93, 124
Kpn2I TCCGGA 1 cut(s) 850
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Kzo9I GATC 6 cut(s) 214, 258, 847, 959, 1053, 1156
LmnI GCTCC 1 cut(s) 537
Lsp1109I GCAGC 4 cut(s) 92, 342, 372, 421
LweI GCATC 4 cut(s) 421, 438, 654, 910
MaeI CTAG 2 cut(s) 204, 467
MaeII ACGT 2 cut(s) 455, 1191
MaeIII GTNAC 3 cut(s) 6, 398, 1119
MalI GATC 6 cut(s) 216, 260, 849, 961, 1055, 1158
MboI GATC 6 cut(s) 214, 258, 847, 959, 1053, 1156
MfeI CAATTG 1 cut(s) 726
MflI RGATCY 1 cut(s) 258
MlyI GAGTC 3 cut(s) 395, 439, 1127
MnlI CCTC 5 cut(s) 279, 469, 501, 614, 1068
MroI TCCGGA 1 cut(s) 850
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 3 cut(s) 682, 697, 974
MseI TTAA 1 cut(s) 806
MslI CAYNNNNRTG 1 cut(s) 998
MspI CCGG 5 cut(s) 102, 114, 385, 418, 851
MspR9I CCNGG 1 cut(s) 419
MunI CAATTG 1 cut(s) 726
MwoI GCNNNNNNNGC 1 cut(s) 144
NaeI GCCGGC 1 cut(s) 103
NciI CCSGG 1 cut(s) 419
NcoI CCATGG 1 cut(s) 1176
NdeII GATC 6 cut(s) 214, 258, 847, 959, 1053, 1156
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 4 cut(s) 39, 897, 1075, 1180
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 2 cut(s) 398, 1119
NsbI TGCGCA 1 cut(s) 125
NspV TTCGAA 1 cut(s) 86
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 3 cut(s) 682, 697, 974
PfeI GAWTC 6 cut(s) 302, 710, 782, 820, 875, 1126
PinAI ACCGGT 1 cut(s) 384
PkrI GCNGC 4 cut(s) 107, 332, 362, 411
PleI GAGTC 3 cut(s) 395, 438, 1126
PpsI GAGTC 3 cut(s) 395, 438, 1126
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 1 cut(s) 908
PstI CTGCAG 2 cut(s) 382, 1099
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
RseI CAYNNNNRTG 1 cut(s) 998
SaqAI TTAA 1 cut(s) 806
SatI GCNGC 4 cut(s) 106, 331, 361, 410
Sau3AI GATC 6 cut(s) 214, 258, 847, 959, 1053, 1156
Sau96I GGNCC 1 cut(s) 908
ScaI AGTACT 1 cut(s) 721
SchI GAGTC 3 cut(s) 395, 439, 1127
ScrFI CCNGG 1 cut(s) 419
SfaNI GCATC 4 cut(s) 421, 438, 654, 910
SfcI CTRYAG 2 cut(s) 378, 1095
SfuI TTCGAA 1 cut(s) 86
SinI GGWCC 1 cut(s) 908
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
SsiI CCGC 1 cut(s) 99
SspMI CTAG 2 cut(s) 204, 467
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 2 cut(s) 210, 1176
TaiI ACGT 2 cut(s) 458, 1194
TaqI TCGA 7 cut(s) 86, 368, 390, 428, 818, 825, 1116
TaqII GACCGA 1 cut(s) 423
TatI WGTACW 1 cut(s) 719
TfiI GAWTC 6 cut(s) 302, 710, 782, 820, 875, 1126
Tru1I TTAA 1 cut(s) 806
Tru9I TTAA 1 cut(s) 806
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 2 cut(s) 398, 1119
TseI GCWGC 4 cut(s) 105, 330, 360, 409
Tsp45I GTSAC 2 cut(s) 398, 1119
TspDTI ATGAA 3 cut(s) 17, 386, 882
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 908
XapI RAATTY 9 cut(s) 117, 129, 190, 346, 373, 479, 974, 1078, 1182
XmnI GAANNNNTTC 3 cut(s) 682, 697, 974
XspI CTAG 2 cut(s) 204, 467
ZrmI AGTACT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.