Rw5G042750

DUF761-associated sequence motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009893
Physical Location & Seq
Reverse (-)
74729385 .. 74730690
1306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042750.1

Sequence Viewer

Length: 1212 bp
ATGAAGTTGCTATCTTCTTCTGAATCCTCTCCTTTTTCTTCTTTTTCTTCGTGTTCATCATCCACCACCACCACTACCACCACTTTTGATCTAACCAATTCAAAAGGTGCTACTAATGGATGCATTGCTGGCATTTTGCGCAGAATTCTCTGCTATGGTAGCTTGCCTACATACCCTTCTGATCATATCTCAGAAGAAACCAATTCTGTCGATCAATCTCAAAATGATAGAGCATTCAAGTCGAAAAACAAAACAGAGGAAACAGAGGCATCTGCCACTCCAAGCTTAGTGGCAAGGCTAATGGGGTTGGAGTCAATTCCAGATGCCAACTTGGTCTCCACCCCAAATTCAATATCCCGTAGCCGGTCTATGAATTCTGTGGACCGGCTTTCGGGAGACCATGATCCAATCGAGGCACATGAGCGCCGACGAGTCAAGTCGTTTAGAGAGATGCCAACATTTATTGAAAATGAGGAATTCCTTGTTCTTAGCTTTGAGAATGGGAGTGAAAGAAAAGAAAGGAGATCGAAGGGGAGGAAATATGAGACGGGCCATGGAGAATTGAGGAAGAAGAAGGCAGGAAAGAGCAGAAACAAGGGAAATAGAAAACAAAGAGGGTTTGAGAAGGAAAAGAAGCAAGTTCAAGAAGAAAAAAGCAGAAGGGTTTTGAAAGATTTGAATGGGAGGGAAATGCTAAGAAGCAAAACCTCTCATAAACCCAAGAAAGAGACCATCTTTGATATGCTTAAGAATGTAGAATCTGATTGTACCTCGGAAGATTTAAGCCCGGTTTCTGTCCTTGATTGTGGTCAGTTTCTAATTGATAACGAAGTTCCTACATCAGAAGAAGATCCAGTCTTGGTGAGTTCATTTCCGAGAAGAAAAGACGACTGTTTAACTTGTGATGCAAGAAAAACAAAGAAAATCGAAGGCATATGTCTTGGAACAAAGAAAGAGTATCACACTGCAGAGTACTTTGAGATTTTGGGTGAAATTTGTACTCTGACTGAAGCTGAATTGGTTGGATCAAATTGGAGCGTACACAAAGGCATCTGGAATAATCATGAAGGTTCTGCAGGAATTGCTGCAGATTTCGAGTCACAAATTCTAGGCCAATTAATAGAGGAGCTCGTGGATCAACTCGCTGATTTTCCTACTGAATATTCAAAACCTGTAAGATTTACGACCATTGTAAATTTGGTATTACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VARLMGL

Protein Analysis

403

Amino Acids

45.2

Weight (kDa)

6.5

Isoelectric Point (pI)

58.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 92 - 110 5e-08 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58630
fragaria_vesca FvH4_3g38760 FvH4_3g38760
malus_domestica MD03G1068300.v1.1 MD11G1072700.v1.1
prunus_persica Prupe.6G056200_v2.0.a1
pyrus_communis pycom03g05470 pycom03g05480 pycom11g06040
rosa_chinensis RchiOBHm_Chr5g0069911
rosa_laevigata RLG00000036107
rosa_multiflora Rmu_ssc0000133.1_g000001
rosa_roxburghii Rroxscaffold_1G00011150
rosa_rugosa Rorug05G0400700
rosa_samantha Rh5AG457800 Rh5BG476600 Rh5BG476700 Rh5CG500400 Rh5CG500500 Rh5DG488500
rosa_wichuraiana Rw5G042750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 140
AclWI GGATC 4 cut(s) 398, 845, 1033, 1143
AcsI RAATTY 7 cut(s) 144, 346, 373, 476, 993, 1104, 1195
AcuI CTGAAG 1 cut(s) 1029
AfaI GTAC 4 cut(s) 769, 974, 1000, 1041
AfiI CCNNNNNNNGG 2 cut(s) 363, 391
AflII CTTAAG 1 cut(s) 746
AgsI TTSAA 8 cut(s) 102, 238, 351, 467, 644, 670, 679, 1167
AluBI AGCT 5 cut(s) 162, 285, 492, 1013, 1129
AluI AGCT 5 cut(s) 162, 285, 492, 1013, 1129
Alw21I GWGCWC 1 cut(s) 1131
Alw26I GTCTC 4 cut(s) 340, 390, 539, 722
AlwI GGATC 4 cut(s) 398, 845, 1033, 1143
AoxI GGCC 2 cut(s) 550, 1111
ApeKI GCWGC 1 cut(s) 1085
ApoI RAATTY 7 cut(s) 144, 346, 373, 476, 993, 1104, 1195
AseI ATTAAT 1 cut(s) 1118
AspLEI GCGC 2 cut(s) 141, 426
AspS9I GGNCC 2 cut(s) 382, 550
AsuC2I CCSGG 1 cut(s) 788
AsuHPI GGTGA 2 cut(s) 874, 1001
AvaII GGWCC 1 cut(s) 382
BanII GRGCYC 1 cut(s) 1131
BauI CACGAG 1 cut(s) 1130
Bbv12I GWGCWC 1 cut(s) 1131
BbvI GCAGC 1 cut(s) 1072
BccI CCATC 1 cut(s) 740
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 1 cut(s) 788
BcoDI GTCTC 4 cut(s) 340, 390, 539, 722
BfaI CTAG 1 cut(s) 1109
BfmI CTRYAG 3 cut(s) 966, 1074, 1086
BfoI RGCGCY 1 cut(s) 427
BfrI CTTAAG 1 cut(s) 746
BisI GCNGC 1 cut(s) 1086
BlsI GCNGC 1 cut(s) 1087
BmcAI AGTACT 1 cut(s) 974
Bme1390I CCNGG 1 cut(s) 788
Bme18I GGWCC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 382, 550
BmrFI CCNGG 1 cut(s) 788
BmsI GCATC 6 cut(s) 110, 278, 313, 441, 895, 1059
BpuMI CCSGG 1 cut(s) 788
BsaI GGTCTC 3 cut(s) 340, 390, 722
BsaJI CCNNGG 2 cut(s) 553, 771
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 363, 391
Bse118I RCCGGY 2 cut(s) 363, 384
Bse1I ACTGG 1 cut(s) 854
Bse3DI GCAATG 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 553, 771
BseGI GGATG 2 cut(s) 59, 125
BseLI CCNNNNNNNGG 2 cut(s) 363, 391
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 854
BseRI GAGGAG 1 cut(s) 1139
BseXI GCAGC 1 cut(s) 1072
BshFI GGCC 2 cut(s) 552, 1113
BsiHKAI GWGCWC 1 cut(s) 1131
BsiSI CCGG 3 cut(s) 364, 385, 788
BslI CCNNNNNNNGG 2 cut(s) 363, 391
BsmAI GTCTC 4 cut(s) 340, 390, 539, 722
BsmBI CGTCTC 1 cut(s) 539
BsmI GAATGC 1 cut(s) 233
BsnI GGCC 2 cut(s) 552, 1113
Bso31I GGTCTC 3 cut(s) 340, 390, 722
Bsp1286I GDGCHC 1 cut(s) 1131
Bsp143I GATC 8 cut(s) 88, 181, 211, 403, 524, 850, 1025, 1135
Bsp19I CCATGG 1 cut(s) 553
BspANI GGCC 2 cut(s) 552, 1113
BspCNI CTCAG 1 cut(s) 203
BspHI TCATGA 1 cut(s) 1063
BspMAI CTGCAG 3 cut(s) 970, 1078, 1090
BspPI GGATC 4 cut(s) 398, 845, 1033, 1143
BspTI CTTAAG 1 cut(s) 746
BspTNI GGTCTC 3 cut(s) 340, 390, 722
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 2 cut(s) 363, 384
BsrI ACTGG 1 cut(s) 854
BssAI RCCGGY 2 cut(s) 363, 384
BssECI CCNNGG 2 cut(s) 553, 771
BssMI GATC 8 cut(s) 88, 181, 211, 403, 524, 850, 1025, 1135
BssSI CACGAG 1 cut(s) 1130
BssT1I CCWWGG 1 cut(s) 553
Bst2BI CACGAG 1 cut(s) 1130
Bst4CI ACNGT 1 cut(s) 893
BstAFI CTTAAG 1 cut(s) 746
BstAPI GCANNNNNTGC 1 cut(s) 1082
BstC8I GCNNGC 2 cut(s) 130, 164
BstDEI CTNAG 4 cut(s) 190, 286, 488, 695
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 2 cut(s) 59, 125
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 2 cut(s) 141, 426
BstKTI GATC 8 cut(s) 91, 184, 214, 406, 527, 853, 1028, 1138
BstMAI GTCTC 4 cut(s) 340, 390, 539, 722
BstMBI GATC 8 cut(s) 88, 181, 211, 403, 524, 850, 1025, 1135
BstMWI GCNNNNNNNGC 4 cut(s) 129, 138, 159, 1082
BstSCI CCNGG 1 cut(s) 786
BstSFI CTRYAG 3 cut(s) 966, 1074, 1086
BstV1I GCAGC 1 cut(s) 1072
BstX2I RGATCY 1 cut(s) 850
BstYI RGATCY 1 cut(s) 850
BsuRI GGCC 2 cut(s) 552, 1113
BtgI CCRYGG 1 cut(s) 553
BtsCI GGATG 2 cut(s) 59, 125
BtsI GCAGTG 1 cut(s) 963
BtsIMutI CAGTG 1 cut(s) 963
Cac8I GCNNGC 2 cut(s) 130, 164
CciI TCATGA 1 cut(s) 1063
CfoI GCGC 2 cut(s) 141, 426
Cfr10I RCCGGY 2 cut(s) 363, 384
Cfr13I GGNCC 2 cut(s) 382, 550
Csp6I GTAC 4 cut(s) 768, 973, 999, 1040
CspCI CAANNNNNGTGG 4 cut(s) 67, 102, 270, 305
CviAII CATG 4 cut(s) 401, 419, 554, 1064
CviQI GTAC 4 cut(s) 768, 973, 999, 1040
DdeI CTNAG 4 cut(s) 190, 286, 488, 695
DpnI GATC 8 cut(s) 90, 183, 213, 405, 526, 852, 1027, 1137
DpnII GATC 8 cut(s) 88, 181, 211, 403, 524, 850, 1025, 1135
Ecl136II GAGCTC 1 cut(s) 1129
Eco130I CCWWGG 1 cut(s) 553
Eco24I GRGCYC 1 cut(s) 1131
Eco31I GGTCTC 3 cut(s) 340, 390, 722
Eco47I GGWCC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 1129
Eco57I CTGAAG 1 cut(s) 1029
EcoICRI GAGCTC 1 cut(s) 1129
EcoRI GAATTC 3 cut(s) 144, 373, 476
EcoT14I CCWWGG 1 cut(s) 553
EcoT22I ATGCAT 1 cut(s) 125
EcoT38I GRGCYC 1 cut(s) 1131
ErhI CCWWGG 1 cut(s) 553
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 4 cut(s) 404, 422, 557, 1067
FatI CATG 4 cut(s) 400, 418, 553, 1063
FauNDI CATATG 1 cut(s) 935
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 1 cut(s) 1086
FokI GGATG 2 cut(s) 46, 132
FriOI GRGCYC 1 cut(s) 1131
Fsp4HI GCNGC 1 cut(s) 1086
FspBI CTAG 1 cut(s) 1109
FspI TGCGCA 1 cut(s) 140
GlaI GCGC 2 cut(s) 140, 425
GluI GCNGC 1 cut(s) 1086
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 2 cut(s) 552, 1113
HapII CCGG 3 cut(s) 364, 385, 788
HhaI GCGC 2 cut(s) 141, 426
Hin1II CATG 4 cut(s) 404, 422, 557, 1067
Hin6I GCGC 2 cut(s) 139, 424
HinP1I GCGC 2 cut(s) 139, 424
HindIII AAGCTT 1 cut(s) 283
HinfI GANTC 5 cut(s) 23, 311, 432, 758, 1097
HpaII CCGG 3 cut(s) 364, 385, 788
HphI GGTGA 2 cut(s) 874, 1001
Hpy166II GTNNAC 2 cut(s) 382, 1042
Hpy188I TCNGA 8 cut(s) 22, 181, 193, 763, 775, 844, 876, 1005
Hpy188III TCNNGA 5 cut(s) 320, 393, 644, 1054, 1064
Hpy8I GTNNAC 2 cut(s) 382, 1042
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 7 cut(s) 186, 523, 568, 619, 654, 923, 1061
HpyCH4III ACNGT 1 cut(s) 893
HpyCH4V TGCA 5 cut(s) 123, 908, 968, 1076, 1088
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 138, 159, 1082
HpyF3I CTNAG 4 cut(s) 190, 286, 488, 695
Hsp92II CATG 4 cut(s) 404, 422, 557, 1067
HspAI GCGC 2 cut(s) 139, 424
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 8 cut(s) 88, 181, 211, 403, 524, 850, 1025, 1135
LmnI GCTCC 2 cut(s) 1035, 1126
Lsp1109I GCAGC 1 cut(s) 1072
LweI GCATC 6 cut(s) 110, 278, 313, 441, 895, 1059
MaeI CTAG 1 cut(s) 1109
MaeIII GTNAC 1 cut(s) 1098
MalI GATC 8 cut(s) 90, 183, 213, 405, 526, 852, 1027, 1137
MboI GATC 8 cut(s) 88, 181, 211, 403, 524, 850, 1025, 1135
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 1 cut(s) 1131
MlyI GAGTC 3 cut(s) 320, 441, 1106
MmeI TCCRAC 2 cut(s) 288, 1003
Mph1103I ATGCAT 1 cut(s) 125
MseI TTAA 4 cut(s) 747, 782, 896, 1118
MspCI CTTAAG 1 cut(s) 746
MspI CCGG 3 cut(s) 364, 385, 788
MspR9I CCNGG 1 cut(s) 788
Mva1269I GAATGC 1 cut(s) 233
MwoI GCNNNNNNNGC 4 cut(s) 129, 138, 159, 1082
NciI CCSGG 1 cut(s) 788
NcoI CCATGG 1 cut(s) 553
NdeI CATATG 1 cut(s) 935
NdeII GATC 8 cut(s) 88, 181, 211, 403, 524, 850, 1025, 1135
NlaIII CATG 4 cut(s) 404, 422, 557, 1067
NmuCI GTSAC 1 cut(s) 1098
NsbI TGCGCA 1 cut(s) 140
NsiI ATGCAT 1 cut(s) 125
PagI TCATGA 1 cut(s) 1063
PctI GAATGC 1 cut(s) 233
PfeI GAWTC 2 cut(s) 23, 758
PkrI GCNGC 1 cut(s) 1087
PleI GAGTC 3 cut(s) 319, 440, 1105
PpsI GAGTC 3 cut(s) 319, 440, 1105
PshBI ATTAAT 1 cut(s) 1118
Psp124BI GAGCTC 1 cut(s) 1131
PspPI GGNCC 2 cut(s) 382, 550
PstI CTGCAG 3 cut(s) 970, 1078, 1090
PsuI RGATCY 1 cut(s) 850
RsaI GTAC 4 cut(s) 769, 974, 1000, 1041
RsaNI GTAC 4 cut(s) 768, 973, 999, 1040
SacI GAGCTC 1 cut(s) 1131
SaqAI TTAA 4 cut(s) 747, 782, 896, 1118
SatI GCNGC 1 cut(s) 1086
Sau3AI GATC 8 cut(s) 88, 181, 211, 403, 524, 850, 1025, 1135
Sau96I GGNCC 2 cut(s) 382, 550
ScaI AGTACT 1 cut(s) 974
SchI GAGTC 3 cut(s) 320, 441, 1106
ScrFI CCNGG 1 cut(s) 788
SduI GDGCHC 1 cut(s) 1131
SfaNI GCATC 6 cut(s) 110, 278, 313, 441, 895, 1059
SfcI CTRYAG 3 cut(s) 966, 1074, 1086
SinI GGWCC 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
SspI AATATT 1 cut(s) 1163
SspMI CTAG 1 cut(s) 1109
SstI GAGCTC 1 cut(s) 1131
StyD4I CCNGG 1 cut(s) 786
StyI CCWWGG 1 cut(s) 553
TaaI ACNGT 1 cut(s) 893
TaqI TCGA 6 cut(s) 210, 242, 411, 527, 927, 1095
TatI WGTACW 2 cut(s) 972, 998
TfiI GAWTC 2 cut(s) 23, 758
Tru1I TTAA 4 cut(s) 747, 782, 896, 1118
Tru9I TTAA 4 cut(s) 747, 782, 896, 1118
TscAI CASTG 1 cut(s) 970
TseFI GTSAC 1 cut(s) 1098
TseI GCWGC 1 cut(s) 1085
Tsp45I GTSAC 1 cut(s) 1098
TspDTI ATGAA 5 cut(s) 17, 45, 386, 858, 1080
TspRI CASTG 1 cut(s) 970
Vha464I CTTAAG 1 cut(s) 746
VpaK11BI GGWCC 1 cut(s) 382
VspI ATTAAT 1 cut(s) 1118
XapI RAATTY 7 cut(s) 144, 346, 373, 476, 993, 1104, 1195
XcmI CCANNNNNNNNNTGG 1 cut(s) 1195
XspI CTAG 1 cut(s) 1109
ZrmI AGTACT 1 cut(s) 974
Zsp2I ATGCAT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.