Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007746

Physical Location & Seq

Genomic Coordinates Reverse (-)

6012089 .. 6018154

Gene Structure 6066 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1074100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGTCGTCGGTCGAGTATTTACGTCCAACAATCCACAATAGGTTTTGCCTGAATTTGAATTCTAATGCTTATCATTGCCGCCATGGATTGGGATTCCTCCGCAGTCAATCGAGGGGTTTCAACCAGGAACCGAGGCGGTGCGTTTCGAACGCGGCGGTGTTTCCTCCGGTCACGTTGCACGGACAGGGTGGCAGAGCTGTGAGAGTTTCCGAGAGGTTCGGCGGCCTCTGGAGGAACCACGGAGGGTTTCGAAAGGTTCGGGTTTCGGCGAGCGGTCAGGACACCGATTCCGGGGAGAAGAGCGAGGCGAAGCCGACTGAGGGCCAGGCTGTGAACAATAACCCGCCAAGTTCGAGCTCGCCGGCTTCAAATCGGCGGCGAGAGAGTCGAAAGAAAGAGAATTGGTGGTGGTCGAAAGGAGGGAAATGGCGGTGGCAGCCGATAGTTCAGGCACAAGAAATTGGAATCTTGCTGTTACAATTGGGTATTGTAATTTTCGTCATGCGGTTGCTCCGGCCCGGAATTCCGTTACCTGGGTCGGAGCCGAGGACGCCCACCACGTTCGTTAGCGTGCCGTACAGTGATTTTCTGAGCAAGATTAATAGTAACCAGGTGCAGAAGGTTGAGGTTGATGGGGTTCATGTTATGTTTAAGTTGAAGTCTGAGCAGGGTGAGCAAGAAAGTGAAGTGAGTGGTGGTGGGGCGAGCAAGTTTCAGGAATCGGAGGCTTTGGTAAGAAGCGTGGCTCCGACAAAGAGGGTGGTTTACACGACGACAAGGCCAACTGATATCAAGACGCCGTATGAGAAAATGCTTGAGAATGAGGTGGAGTTCGGGTCACCGGATAAACGGTCAGGCGGATTCTTGAACTCTGCAATGATAGCTTTGTTTTATGCTGCTGTGCTTGCAGGGCTTCTCCACAGGTTCCCTGTTAACTTTACTCAGCAAACAGCTGGTCAGATTAGGAACAGGAAATCTGGGGGCTCTGCTGGTGCTAAAGCATCCGAACAAGGGGAAGCAATAACCTTTGCTGATGTTGCTGGCGTTGATGAGGCCAAAGCGGAGCTAGAAGAAATTGTGGAATTTCTTCGGAATCCAGACAAGTATATACGACTTGGTGCTCGTCCTCCACGTGGAGTTCTCCTGGTGGGTCTTCCTGGGACAGGTAAGACTCTTCTTGCCAAGGCTGTGGCTGGGGAAGCTGATGTGCCCTTTATAAGTTGCTCTGCTAGTGAGTTTGTAGAGTTGTATGTTGGCATGGGTGCCTCTCGTGTAAGGGATCTATTTGCACGGGCAAAAAAGGAGTCACCATCCATAATATTTATTGATGAGATAGATGCTGTGGCAAAAAGCCGTGATGGAAAATTCCGTATTGTCAGCAATGATGAGCGAGAACAAACCTTGAATCAGTTACTCACTGAGATGGATGGATTTGACAGCAACTCTGCGGTTATTGTTCTTGGAGCAACTAATCGCGCCGATGTCTTAGACCCAGCACTTCGTCGACCAGGGAGATTTGATCGTGTGGTTATGGTGGAAACACCAGATAGGAGAGGAAGAGAAGAGATTCTAAAGGTGCATGCTACCAAAAAAGAACTTCCTTTGGCAAAAGATGTCTACCTTGGTGACATTGCTTCTATGACCACTGGCTTTACTGGGGCAGATCTCGCAAATTTGGTGAATGAAGCTGCTTTATTGGCTGGAAGACAAAGCAAGTTGGTTGTGGAAAAAATTGATTTTATTCAAGCTGTGGAGAGATTAATAGCTGGTATAGAAAAGAAGACTGCCAAGTTGCAGGGAAGTGAGAAGGCTGTAGTTGCACGACATGAAGCTGGTCATGCAGTAGTGGGGACTGCTGTTGCAAGCCTTCTTCCTGGACAGCCACGTGTTGAGAAATTGAGCATATTGCCAAGATCAGGAGGGGCGTTGGGCTTCACTTATATTCCTCCAACAACGGAAGATAGATATTTACTCTTCATTGATGAGTTGCGTGGACGTTTGGTTACACTTCTTGGAGGACGTGCAGCAGAAGAATTTGTATATTCAGGTCGTGTCTCAACGGGTGCACTTGATGATATACGACGAGCAACTGACATGGCATACAAAGCAGTAGCTGAGTATGGTCTCAATCACAACATTGGCCCTGTGTCTATAGCAACACTTTCTGCTGGTGGAATGGATGAGTCTGGAGGAGGTGCTCTTTGGGGAAGGGATCAGGGGCATCTTGTTGATCTTGTTCAAGGAGAGGTTAAAGCTTTGCTACAATCTGCTTTGGATATAGCTCTTTCGGTTGTACGTGCTAATCCTACTGTTTTGGAGGGTCTTGGTGCTCAGTTGGAAGAAAAAGAAAAGGTAGAAGGCGAAGAGCTACAAAAGTGGTTAAAATTGGTGGTTGCACCAACGGAACTTTCAATCTTCATTAGTGGCAAGCAAGAGTCTCTTCCCTCATTGCAGACCATCTCTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0031967 GO:0031975 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA FtsH_ext Peptidase_M41

Protein Analysis

823

Amino Acids

89.31

Weight (kDa)

8.9

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsH_ext	PF06480	161 - 241	9.2e-06	FtsH Extracellular
AAA	PF00004	380 - 513	1.7e-43	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	538 - 580	2.1e-10	AAA+ lid domain
Peptidase_M41	PF01434	599 - 792	6e-35	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007746)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47060 AT5G58870
fragaria_vesca	FvH4_3g38190 FvH4_3g38190 FvH4_3g38190
malus_domestica	MD03G1074100.v1.1 MD11G1080400.v1.1
prunus_persica	Prupe.6G060800_v2.0.a1 Prupe.6G060800_v2.0.a1 Prupe.6G060800_v2.0.a1
pyrus_communis	pycom03g05990 pycom11g06770
rosa_chinensis	RchiOBHm_Chr5g0068621
rosa_laevigata	RLG00000035985
rosa_multiflora	Rmu_co8416215.1_g000001 Rmu_sc0000376.1_g000003 Rmu_sc0004721.1_g000004
rosa_roxburghii	Rroxscaffold_1G00012250
rosa_rugosa	Rorug05G0392000
rosa_samantha	Rh5AG449600 Rh5BG467800 Rh5CG489600 Rh5DG481200
rosa_wichuraiana	Rw5G041980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1217
AccB1I	GGYRCC	1 cut(s)	1262
AccB7I	CCANNNNNTGG	1 cut(s)	88
AccBSI	CCGCTC	1 cut(s)	273
AccI	GTMKAC	2 cut(s)	1504, 1617
AccII	CGCG	2 cut(s)	152, 1476
AclWI	GGATC	2 cut(s)	1287, 2220
AcsI	RAATTY	7 cut(s)	52, 58, 522, 1082, 1364, 1672, 2033
AcvI	CACGTG	2 cut(s)	1133, 1886
AcyI	GRCGYC	2 cut(s)	551, 797
AfaI	GTAC	2 cut(s)	578, 2295
AfiI	CCNNNNNNNGG	8 cut(s)	88, 291, 320, 533, 1011, 1133, 1163, 1916
AflIII	ACRYGT	1 cut(s)	1885
AgsI	TTSAA	9 cut(s)	58, 121, 369, 658, 868, 1405, 1745, 2240, 2412
AjiI	CACGTC	1 cut(s)	2021
AjnI	CCWGG	8 cut(s)	123, 324, 532, 609, 1143, 1156, 1507, 1873
AjuI	GAANNNNNNNTTGG	2 cut(s)	2392, 2424
Alw21I	GWGCWC	5 cut(s)	359, 1123, 2068, 2200, 2332
Alw26I	GTCTC	3 cut(s)	2059, 2129, 2442
Alw44I	GTGCAC	1 cut(s)	2064
AlwI	GGATC	2 cut(s)	1287, 2220
AlwNI	CAGNNNCTG	1 cut(s)	2114
AoxI	GGCC	6 cut(s)	223, 322, 515, 779, 1053, 2140
ApaLI	GTGCAC	1 cut(s)	2064
ApeKI	GCWGC	4 cut(s)	436, 896, 1688, 2024
ApoI	RAATTY	7 cut(s)	52, 58, 522, 1082, 1364, 1672, 2033
ArsI	GACNNNNNNTTYG	4 cut(s)	940, 972, 1289, 1321
AseI	ATTAAT	2 cut(s)	600, 1760
Asp700I	GAANNNNTTC	2 cut(s)	1086, 1566
AspLEI	GCGC	1 cut(s)	1478
AspS9I	GGNCC	3 cut(s)	322, 516, 2141
AsuC2I	CCSGG	2 cut(s)	292, 519
AsuHPI	GGTGA	5 cut(s)	683, 831, 1299, 1637, 1690
AsuII	TTCGAA	2 cut(s)	146, 250
BaeGI	GKGCMC	2 cut(s)	1212, 2068
BanI	GGYRCC	1 cut(s)	1262
BanII	GRGCYC	2 cut(s)	359, 986
BauI	CACGAG	1 cut(s)	1269
BbrPI	CACGTG	2 cut(s)	1133, 1886
BbsI	GAAGAC	3 cut(s)	1145, 1711, 1787
Bbv12I	GWGCWC	5 cut(s)	359, 1123, 2068, 2200, 2332
BbvI	GCAGC	4 cut(s)	448, 883, 1675, 2036
BccI	CCATC	6 cut(s)	626, 1318, 1352, 1417, 1421, 2465
BceAI	ACGGC	3 cut(s)	559, 784, 1338
BciT130I	CCWGG	8 cut(s)	125, 326, 534, 611, 1145, 1158, 1509, 1875
BcnI	CCSGG	2 cut(s)	292, 519
BcoDI	GTCTC	3 cut(s)	2059, 2129, 2442
BfaI	CTAG	2 cut(s)	1067, 1230
BfmI	CTRYAG	2 cut(s)	1812, 2151
BglII	AGATCT	1 cut(s)	1663
BisI	GCNGC	8 cut(s)	79, 153, 223, 377, 437, 897, 1689, 2025
BlsI	GCNGC	8 cut(s)	80, 154, 224, 378, 438, 898, 1690, 2026
BmgBI	CACGTC	1 cut(s)	2021
BmgT120I	GGNCC	3 cut(s)	322, 516, 2141
BmiI	GGNNCC	6 cut(s)	129, 236, 543, 747, 926, 1264
BmrI	ACTGGG	1 cut(s)	1665
BmsI	GCATC	3 cut(s)	1010, 1327, 2230
BmuI	ACTGGG	1 cut(s)	1665
BpiI	GAAGAC	3 cut(s)	1145, 1711, 1787
BpmI	CTGGAG	2 cut(s)	250, 2208
Bpu14I	TTCGAA	2 cut(s)	146, 250
BpuEI	CTTGAG	1 cut(s)	836
BpuMI	CCSGG	2 cut(s)	292, 519
BsaAI	YACGTR	3 cut(s)	1133, 1886, 2297
BsaHI	GRCGYC	2 cut(s)	551, 797
BsaI	GGTCTC	1 cut(s)	2129
BsaWI	WCCGGW	2 cut(s)	166, 841
BsaXI	ACNNNNNCTCC	2 cut(s)	821, 851
Bsc4I	CCNNNNNNNGG	8 cut(s)	88, 291, 320, 533, 1011, 1133, 1163, 1916
Bse118I	RCCGGY	1 cut(s)	361
Bse1I	ACTGG	2 cut(s)	1651, 1660
Bse3DI	GCAATG	5 cut(s)	73, 882, 1387, 1629, 2447
BseBI	CCWGG	8 cut(s)	125, 326, 534, 611, 1145, 1158, 1509, 1875
BseGI	GGATG	4 cut(s)	1001, 1310, 1432, 2185
BseLI	CCNNNNNNNGG	8 cut(s)	88, 291, 320, 533, 1011, 1133, 1163, 1916
BseMI	GCAATG	5 cut(s)	73, 882, 1387, 1629, 2447
BseMII	CTCAG	7 cut(s)	309, 581, 654, 956, 1410, 2106, 2345
BseNI	ACTGG	2 cut(s)	1651, 1660
BseRI	GAGGAG	1 cut(s)	2205
BseSI	GKGCMC	2 cut(s)	1212, 2068
BseXI	GCAGC	4 cut(s)	448, 883, 1675, 2036
BseYI	CCCAGC	2 cut(s)	1193, 1492
BsgI	GTGCAG	2 cut(s)	635, 2043
Bsh1236I	CGCG	2 cut(s)	152, 1476
Bsh1285I	CGRYCG	1 cut(s)	12
BshFI	GGCC	6 cut(s)	225, 324, 517, 781, 1055, 2142
BshNI	GGYRCC	1 cut(s)	1262
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	5 cut(s)	359, 1123, 2068, 2200, 2332
BsiSI	CCGG	6 cut(s)	167, 291, 362, 514, 519, 842
BslFI	GGGAC	2 cut(s)	1174, 1864
BslI	CCNNNNNNNGG	8 cut(s)	88, 291, 320, 533, 1011, 1133, 1163, 1916
BsmAI	GTCTC	3 cut(s)	2059, 2129, 2442
BsmFI	GGGAC	2 cut(s)	1174, 1864
BsnI	GGCC	6 cut(s)	225, 324, 517, 781, 1055, 2142
Bso31I	GGTCTC	1 cut(s)	2129
Bsp119I	TTCGAA	2 cut(s)	146, 250
Bsp1286I	GDGCHC	7 cut(s)	359, 986, 1123, 1212, 2068, 2200, 2332
Bsp143I	GATC	6 cut(s)	1279, 1519, 1663, 1913, 2212, 2230
Bsp19I	CCATGG	1 cut(s)	82
BspANI	GGCC	6 cut(s)	225, 324, 517, 781, 1055, 2142
BspCNI	CTCAG	7 cut(s)	310, 582, 655, 955, 1411, 2107, 2344
BspFNI	CGCG	2 cut(s)	152, 1476
BspLI	GGNNCC	6 cut(s)	129, 236, 543, 747, 926, 1264
BspPI	GGATC	2 cut(s)	1287, 2220
BspQI	GCTCTTC	2 cut(s)	293, 2358
BspT104I	TTCGAA	2 cut(s)	146, 250
BspT107I	GGYRCC	1 cut(s)	1262
BspTNI	GGTCTC	1 cut(s)	2129
BsrBI	CCGCTC	1 cut(s)	273
BsrDI	GCAATG	5 cut(s)	73, 882, 1387, 1629, 2447
BsrFI	RCCGGY	1 cut(s)	361
BsrI	ACTGG	2 cut(s)	1651, 1660
BssAI	RCCGGY	1 cut(s)	361
BssMI	GATC	6 cut(s)	1279, 1519, 1663, 1913, 2212, 2230
BssNI	GRCGYC	2 cut(s)	551, 797
BssSI	CACGAG	1 cut(s)	1269
BssT1I	CCWWGG	3 cut(s)	82, 1182, 1621
Bst2BI	CACGAG	1 cut(s)	1269
Bst2UI	CCWGG	8 cut(s)	125, 326, 534, 611, 1145, 1158, 1509, 1875
Bst4CI	ACNGT	3 cut(s)	581, 852, 2311
Bst6I	CTCTTC	7 cut(s)	293, 1179, 1552, 1557, 1979, 2358, 2445
BstACI	GRCGYC	2 cut(s)	551, 797
BstBAI	YACGTR	3 cut(s)	1133, 1886, 2297
BstBI	TTCGAA	2 cut(s)	146, 250
BstDEI	CTNAG	8 cut(s)	318, 590, 663, 942, 1419, 1486, 2115, 2331
BstDSI	CCRYGG	2 cut(s)	82, 238
BstEII	GGTNACC	1 cut(s)	837
BstENI	CCTNNNNNAGG	1 cut(s)	1161
BstF5I	GGATG	4 cut(s)	1001, 1310, 1432, 2185
BstFNI	CGCG	2 cut(s)	152, 1476
BstHHI	GCGC	1 cut(s)	1478
BstKTI	GATC	6 cut(s)	1282, 1522, 1666, 1916, 2215, 2233
BstMAI	GTCTC	3 cut(s)	2059, 2129, 2442
BstMBI	GATC	6 cut(s)	1279, 1519, 1663, 1913, 2212, 2230
BstMCI	CGRYCG	1 cut(s)	12
BstNI	CCWGG	8 cut(s)	125, 326, 534, 611, 1145, 1158, 1509, 1875
BstNSI	RCATGY	1 cut(s)	1583
BstPI	GGTNACC	1 cut(s)	837
BstSFI	CTRYAG	2 cut(s)	1812, 2151
BstSLI	GKGCMC	2 cut(s)	1212, 2068
BstUI	CGCG	2 cut(s)	152, 1476
BstV1I	GCAGC	4 cut(s)	448, 883, 1675, 2036
BstV2I	GAAGAC	3 cut(s)	1145, 1711, 1787
BstX2I	RGATCY	2 cut(s)	1279, 1663
BstXI	CCANNNNNNTGG	1 cut(s)	1189
BstYI	RGATCY	2 cut(s)	1279, 1663
BsuRI	GGCC	6 cut(s)	225, 324, 517, 781, 1055, 2142
BtgI	CCRYGG	2 cut(s)	82, 238
BtrI	CACGTC	1 cut(s)	2021
BtsCI	GGATG	4 cut(s)	1001, 1310, 1432, 2185
BtsIMutI	CAGTG	3 cut(s)	586, 1416, 1644
CaiI	CAGNNNCTG	1 cut(s)	2114
CfoI	GCGC	1 cut(s)	1478
Cfr10I	RCCGGY	1 cut(s)	361
Cfr13I	GGNCC	3 cut(s)	322, 516, 2141
CseI	GACGC	2 cut(s)	559, 805
CsiI	ACCWGGT	1 cut(s)	609
Csp6I	GTAC	2 cut(s)	577, 2294
CspCI	CAANNNNNGTGG	2 cut(s)	741, 776
CviAII	CATG	8 cut(s)	83, 502, 641, 1258, 1580, 1826, 1838, 2095
CviQI	GTAC	2 cut(s)	577, 2294
DdeI	CTNAG	8 cut(s)	318, 590, 663, 942, 1419, 1486, 2115, 2331
DpnI	GATC	6 cut(s)	1281, 1521, 1665, 1915, 2214, 2232
DpnII	GATC	6 cut(s)	1279, 1519, 1663, 1913, 2212, 2230
Eam1104I	CTCTTC	7 cut(s)	293, 1179, 1552, 1557, 1979, 2358, 2445
EarI	CTCTTC	7 cut(s)	293, 1179, 1552, 1557, 1979, 2358, 2445
EciI	GGCGGA	1 cut(s)	873
Ecl136II	GAGCTC	1 cut(s)	357
Eco130I	CCWWGG	3 cut(s)	82, 1182, 1621
Eco24I	GRGCYC	2 cut(s)	359, 986
Eco31I	GGTCTC	1 cut(s)	2129
Eco32I	GATATC	1 cut(s)	790
Eco53kI	GAGCTC	1 cut(s)	357
Eco72I	CACGTG	2 cut(s)	1133, 1886
Eco91I	GGTNACC	1 cut(s)	837
EcoICRI	GAGCTC	1 cut(s)	357
EcoNI	CCTNNNNNAGG	1 cut(s)	1161
EcoO65I	GGTNACC	1 cut(s)	837
EcoRI	GAATTC	2 cut(s)	58, 522
EcoRII	CCWGG	8 cut(s)	123, 324, 532, 609, 1143, 1156, 1507, 1873
EcoRV	GATATC	1 cut(s)	790
EcoT14I	CCWWGG	3 cut(s)	82, 1182, 1621
EcoT38I	GRGCYC	2 cut(s)	359, 986
ErhI	CCWWGG	3 cut(s)	82, 1182, 1621
FaeI	CATG	8 cut(s)	86, 505, 644, 1261, 1583, 1829, 1841, 2098
FalI	AAGNNNNNCTT	2 cut(s)	2424, 2456
FaqI	GGGAC	2 cut(s)	1174, 1864
FatI	CATG	8 cut(s)	82, 501, 640, 1257, 1579, 1825, 1837, 2094
FauI	CCCGC	1 cut(s)	351
FblI	GTMKAC	2 cut(s)	1504, 1617
Fnu4HI	GCNGC	8 cut(s)	79, 153, 223, 377, 437, 897, 1689, 2025
FokI	GGATG	4 cut(s)	988, 1297, 1439, 2192
FriOI	GRGCYC	2 cut(s)	359, 986
Fsp4HI	GCNGC	8 cut(s)	79, 153, 223, 377, 437, 897, 1689, 2025
FspBI	CTAG	2 cut(s)	1067, 1230
GlaI	GCGC	1 cut(s)	1477
GluI	GCNGC	8 cut(s)	79, 153, 223, 377, 437, 897, 1689, 2025
GsaI	CCCAGC	2 cut(s)	1197, 1496
GsuI	CTGGAG	2 cut(s)	250, 2208
HaeIII	GGCC	6 cut(s)	225, 324, 517, 781, 1055, 2142
HapII	CCGG	6 cut(s)	167, 291, 362, 514, 519, 842
HgaI	GACGC	2 cut(s)	559, 805
HhaI	GCGC	1 cut(s)	1478
Hin1I	GRCGYC	2 cut(s)	551, 797
Hin1II	CATG	8 cut(s)	86, 505, 644, 1261, 1583, 1829, 1841, 2098
Hin6I	GCGC	1 cut(s)	1476
HinP1I	GCGC	1 cut(s)	1476
HincII	GTYRAC	2 cut(s)	934, 1505
HindII	GTYRAC	2 cut(s)	934, 1505
HindIII	AAGCTT	1 cut(s)	2253
HpaI	GTTAAC	1 cut(s)	934
HpaII	CCGG	6 cut(s)	167, 291, 362, 514, 519, 842
HphI	GGTGA	5 cut(s)	683, 831, 1299, 1637, 1690
Hpy166II	GTNNAC	7 cut(s)	334, 766, 934, 1505, 1618, 1994, 2066
Hpy188I	TCNGA	9 cut(s)	211, 541, 591, 664, 724, 750, 960, 1006, 1092
Hpy188III	TCNNGA	8 cut(s)	229, 278, 716, 793, 865, 1097, 1917, 2187
Hpy8I	GTNNAC	7 cut(s)	334, 766, 934, 1505, 1618, 1994, 2066
Hpy99I	CGWCG	4 cut(s)	10, 775, 1506, 2085
HpyAV	CCTTC	5 cut(s)	613, 1801, 1877, 2202, 2351
HpyCH4III	ACNGT	3 cut(s)	581, 852, 2311
HpyCH4IV	ACGT	8 cut(s)	22, 173, 560, 1132, 1885, 1996, 2020, 2296
HpyF3I	CTNAG	8 cut(s)	318, 590, 663, 942, 1419, 1486, 2115, 2331
HpySE526I	ACGT	8 cut(s)	22, 173, 560, 1132, 1885, 1996, 2020, 2296
Hsp92I	GRCGYC	2 cut(s)	551, 797
Hsp92II	CATG	8 cut(s)	86, 505, 644, 1261, 1583, 1829, 1841, 2098
HspAI	GCGC	1 cut(s)	1476
KroI	GCCGGC	1 cut(s)	361
KroNI	GCCGGC	1 cut(s)	363
KspAI	GTTAAC	1 cut(s)	934
Kzo9I	GATC	6 cut(s)	1279, 1519, 1663, 1913, 2212, 2230
LguI	GCTCTTC	2 cut(s)	293, 2358
LmnI	GCTCC	5 cut(s)	516, 541, 751, 1063, 1463
Lsp1109I	GCAGC	4 cut(s)	448, 883, 1675, 2036
LweI	GCATC	3 cut(s)	1010, 1327, 2230
MabI	ACCWGGT	1 cut(s)	609
MaeI	CTAG	2 cut(s)	1067, 1230
MaeII	ACGT	8 cut(s)	22, 173, 560, 1132, 1885, 1996, 2020, 2296
MaeIII	GTNAC	9 cut(s)	169, 474, 528, 605, 837, 1305, 1410, 1625, 2002
MalI	GATC	6 cut(s)	1281, 1521, 1665, 1915, 2214, 2232
MbiI	CCGCTC	1 cut(s)	273
MboI	GATC	6 cut(s)	1279, 1519, 1663, 1913, 2212, 2230
MfeI	CAATTG	1 cut(s)	479
MflI	RGATCY	2 cut(s)	1279, 1663
MhlI	GDGCHC	7 cut(s)	359, 986, 1123, 1212, 2068, 2200, 2332
MlyI	GAGTC	5 cut(s)	394, 1165, 1313, 2192, 2444
MmeI	TCCRAC	5 cut(s)	50, 519, 773, 1973, 2316
MroNI	GCCGGC	1 cut(s)	361
MroXI	GAANNNNTTC	2 cut(s)	1086, 1566
MseI	TTAA	7 cut(s)	600, 651, 933, 1760, 2250, 2381, 2467
MslI	CAYNNNNRTG	1 cut(s)	1421
MspA1I	CMGCKG	1 cut(s)	953
MspI	CCGG	6 cut(s)	167, 291, 362, 514, 519, 842
MunI	CAATTG	1 cut(s)	479
MvaI	CCWGG	8 cut(s)	125, 326, 534, 611, 1145, 1158, 1509, 1875
MvnI	CGCG	2 cut(s)	152, 1476
NaeI	GCCGGC	1 cut(s)	363
NciI	CCSGG	2 cut(s)	292, 519
NcoI	CCATGG	1 cut(s)	82
NdeII	GATC	6 cut(s)	1279, 1519, 1663, 1913, 2212, 2230
NgoMIV	GCCGGC	1 cut(s)	361
NlaIII	CATG	8 cut(s)	86, 505, 644, 1261, 1583, 1829, 1841, 2098
NlaIV	GGNNCC	6 cut(s)	129, 236, 543, 747, 926, 1264
NmeAIII	GCCGAG	1 cut(s)	570
NmuCI	GTSAC	4 cut(s)	169, 837, 1305, 1625
NspI	RCATGY	1 cut(s)	1583
NspV	TTCGAA	2 cut(s)	146, 250
PaeI	GCATGC	1 cut(s)	1583
PciSI	GCTCTTC	2 cut(s)	293, 2358
PcsI	WCGNNNNNNNCGW	2 cut(s)	557, 1129
PdiI	GCCGGC	1 cut(s)	363
PdmI	GAANNNNTTC	2 cut(s)	1086, 1566
PfeI	GAWTC	8 cut(s)	93, 287, 465, 719, 861, 1093, 1405, 1567
PflMI	CCANNNNNTGG	1 cut(s)	88
PfoI	TCCNGGA	1 cut(s)	1873
PkrI	GCNGC	8 cut(s)	80, 154, 224, 378, 438, 898, 1690, 2026
PleI	GAGTC	5 cut(s)	393, 1165, 1312, 2191, 2443
PmaCI	CACGTG	2 cut(s)	1133, 1886
PmlI	CACGTG	2 cut(s)	1133, 1886
PpsI	GAGTC	5 cut(s)	393, 1165, 1312, 2191, 2443
Ppu21I	YACGTR	3 cut(s)	1133, 1886, 2297
PshBI	ATTAAT	2 cut(s)	600, 1760
PsiI	TTATAA	1 cut(s)	1217
Psp124BI	GAGCTC	1 cut(s)	359
Psp6I	CCWGG	8 cut(s)	123, 324, 532, 609, 1143, 1156, 1507, 1873
PspCI	CACGTG	2 cut(s)	1133, 1886
PspEI	GGTNACC	1 cut(s)	837
PspFI	CCCAGC	2 cut(s)	1193, 1492
PspGI	CCWGG	8 cut(s)	123, 324, 532, 609, 1143, 1156, 1507, 1873
PspN4I	GGNNCC	6 cut(s)	129, 236, 543, 747, 926, 1264
PspPI	GGNCC	3 cut(s)	322, 516, 2141
PstNI	CAGNNNCTG	1 cut(s)	2114
PsuI	RGATCY	2 cut(s)	1279, 1663
PvuII	CAGCTG	1 cut(s)	953
RsaI	GTAC	2 cut(s)	578, 2295
RsaNI	GTAC	2 cut(s)	577, 2294
RseI	CAYNNNNRTG	1 cut(s)	1421
SacI	GAGCTC	1 cut(s)	359
SalI	GTCGAC	1 cut(s)	1503
SapI	GCTCTTC	2 cut(s)	293, 2358
SaqAI	TTAA	7 cut(s)	600, 651, 933, 1760, 2250, 2381, 2467
SatI	GCNGC	8 cut(s)	79, 153, 223, 377, 437, 897, 1689, 2025
Sau3AI	GATC	6 cut(s)	1279, 1519, 1663, 1913, 2212, 2230
Sau96I	GGNCC	3 cut(s)	322, 516, 2141
SchI	GAGTC	5 cut(s)	394, 1165, 1313, 2192, 2444
SduI	GDGCHC	7 cut(s)	359, 986, 1123, 1212, 2068, 2200, 2332
SexAI	ACCWGGT	1 cut(s)	609
SfaNI	GCATC	3 cut(s)	1010, 1327, 2230
SfcI	CTRYAG	2 cut(s)	1812, 2151
SfuI	TTCGAA	2 cut(s)	146, 250
SmiMI	CAYNNNNRTG	1 cut(s)	1421
SmlI	CTYRAG	1 cut(s)	815
SmoI	CTYRAG	1 cut(s)	815
SphI	GCATGC	1 cut(s)	1583
SspI	AATATT	1 cut(s)	1320
SspMI	CTAG	2 cut(s)	1067, 1230
SstI	GAGCTC	1 cut(s)	359
StyI	CCWWGG	3 cut(s)	82, 1182, 1621
TaaI	ACNGT	3 cut(s)	581, 852, 2311
TaiI	ACGT	8 cut(s)	25, 176, 563, 1135, 1888, 1999, 2023, 2299
TaqI	TCGA	8 cut(s)	12, 110, 146, 250, 353, 388, 413, 1504
TauI	GCSGC	4 cut(s)	81, 155, 225, 379
TfiI	GAWTC	8 cut(s)	93, 287, 465, 719, 861, 1093, 1405, 1567
Tru1I	TTAA	7 cut(s)	600, 651, 933, 1760, 2250, 2381, 2467
Tru9I	TTAA	7 cut(s)	600, 651, 933, 1760, 2250, 2381, 2467
TscAI	CASTG	3 cut(s)	586, 1423, 1651
TseFI	GTSAC	4 cut(s)	169, 837, 1305, 1625
TseI	GCWGC	4 cut(s)	436, 896, 1688, 2024
Tsp45I	GTSAC	4 cut(s)	169, 837, 1305, 1625
TspDTI	ATGAA	5 cut(s)	629, 1698, 1842, 1966, 2407
TspGWI	ACGGA	6 cut(s)	195, 255, 516, 1358, 1970, 2417
TspRI	CASTG	3 cut(s)	586, 1423, 1651
Van91I	CCANNNNNTGG	1 cut(s)	88
VneI	GTGCAC	1 cut(s)	2064
VspI	ATTAAT	2 cut(s)	600, 1760
XagI	CCTNNNNNAGG	1 cut(s)	1161
XapI	RAATTY	7 cut(s)	52, 58, 522, 1082, 1364, 1672, 2033
XceI	RCATGY	1 cut(s)	1583
XmiI	GTMKAC	2 cut(s)	1504, 1617
XmnI	GAANNNNTTC	2 cut(s)	1086, 1566
XspI	CTAG	2 cut(s)	1067, 1230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.