MD03G1081300.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005548
Physical Location & Seq
Forward (+)
6558724 .. 6559509
786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1081300.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGAAATTGTAATTTCAATTGCCTCAAAAATTGGAGAGTACTTGGTCGCACCAATAGGGGCAGAGTTTGGCTATTTGATTAATTACCATTCCAACCTCGAAAGTCTTAAGGGTGAGATAAAAAAGCTATTTGAGAAGAAAGATAGAGTGCAAGGATTGGTAAATGCTGCCGAAAGGAACGGTGAAGTTATCAACCCTGATGTTCAGAGTTGGCTAACGAATGTGAACGACTACATGATCCAAAAAGTGTCGCATTTTGAGGATGAAATCAACAAGAAACAGCGATGTATGTATCGATGGAGCTTGAGTCGGAAAGCATATAAGATTACACAACATGTTCTTCAGCTTCAAAACGAAGGAACGTTCAATGATGTGGCCCATCCTGCACCTCCACGAGAGATATGGTCAACTTTCAAAGAAGGTTTTAAGGATTTTAAGTCCAGAATGGCAATCATGAATGAGGTGATAGAGGGTTTGAAGAAGGAAGAAGTACGAATGATCGGGATTTGTGGAATGGGGGGTGTGGGTAAAACCACAATGGCAAAGGAAATTATCAAAAGATTGGCAGAACTGAACCTGTTTGACAAAGTTGTAATGGCAACTGTGTCTCAAAGTCCAAGTACTAGGAGGATTCAGGAGGAAATTGCAGACCAAATAGATTTGAAATTTGATGTGGAATCCGACCCTGGAAGAGTAGGAAGCCTACATGGGAGACTCATGAAAATAGAGAGGATCCTGATAGTATTAGATGATTTATGGACAGAGCTTGATTTTGAGGCTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

262

Amino Acids

29.86

Weight (kDa)

6.04

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 151 - 261 9.9e-20 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 362
AclWI GGATC 3 cut(s) 232, 727, 740
AcsI RAATTY 1 cut(s) 665
AcuI CTGAAG 1 cut(s) 326
AfaI GTAC 3 cut(s) 41, 492, 622
AflII CTTAAG 1 cut(s) 107
AflIII ACRYGT 1 cut(s) 334
AgsI TTSAA 6 cut(s) 18, 350, 367, 415, 478, 664
AjnI CCWGG 1 cut(s) 685
AluBI AGCT 4 cut(s) 127, 303, 346, 766
AluI AGCT 4 cut(s) 127, 303, 346, 766
Alw26I GTCTC 2 cut(s) 612, 706
AlwI GGATC 3 cut(s) 232, 727, 740
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 1 cut(s) 167
ApoI RAATTY 1 cut(s) 665
AseI ATTAAT 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 3 cut(s) 125, 194, 475
BamHI GGATCC 1 cut(s) 732
BauI CACGAG 1 cut(s) 393
BbvI GCAGC 1 cut(s) 154
BccI CCATC 2 cut(s) 291, 387
BciT130I CCWGG 1 cut(s) 687
BcoDI GTCTC 2 cut(s) 612, 706
BfaI CTAG 1 cut(s) 624
BfrI CTTAAG 1 cut(s) 107
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
BmcAI AGTACT 2 cut(s) 41, 622
Bme1390I CCNGG 1 cut(s) 687
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 734
BmrFI CCNGG 1 cut(s) 687
BpuEI CTTGAG 1 cut(s) 325
Bsa29I ATCGAT 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 685
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
BseBI CCWGG 1 cut(s) 687
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 1 cut(s) 685
BseGI GGATG 2 cut(s) 268, 379
BseXI GCAGC 1 cut(s) 154
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 295
BsmAI GTCTC 2 cut(s) 612, 706
BsnI GGCC 1 cut(s) 377
Bsp143I GATC 3 cut(s) 237, 498, 732
BspANI GGCC 1 cut(s) 377
BspDI ATCGAT 1 cut(s) 295
BspHI TCATGA 2 cut(s) 453, 717
BspLI GGNNCC 1 cut(s) 734
BspPI GGATC 3 cut(s) 232, 727, 740
BspTI CTTAAG 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 685
BssMI GATC 3 cut(s) 237, 498, 732
BssSI CACGAG 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 2 cut(s) 182, 604
Bst6I CTCTTC 1 cut(s) 685
BstAFI CTTAAG 1 cut(s) 107
BstF5I GGATG 2 cut(s) 268, 379
BstKTI GATC 3 cut(s) 240, 501, 735
BstMAI GTCTC 2 cut(s) 612, 706
BstMBI GATC 3 cut(s) 237, 498, 732
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 687
BstNSI RCATGY 1 cut(s) 338
BstSCI CCNGG 1 cut(s) 685
BstV1I GCAGC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 732
BstYI RGATCY 1 cut(s) 732
Bsu15I ATCGAT 1 cut(s) 295
BsuRI GGCC 1 cut(s) 377
BsuTUI ATCGAT 1 cut(s) 295
BtgZI GCGATG 1 cut(s) 298
BtsCI GGATG 2 cut(s) 268, 379
CciI TCATGA 2 cut(s) 453, 717
Cfr13I GGNCC 1 cut(s) 376
ClaI ATCGAT 1 cut(s) 295
Csp6I GTAC 3 cut(s) 40, 491, 621
CviAII CATG 5 cut(s) 235, 335, 454, 707, 718
CviJI RGCY 9 cut(s) 72, 127, 214, 303, 346, 377, 702, 766, 779
CviKI_1 RGCY 9 cut(s) 72, 127, 214, 303, 346, 377, 702, 766, 779
CviQI GTAC 3 cut(s) 40, 491, 621
DpnI GATC 3 cut(s) 239, 500, 734
DpnII GATC 3 cut(s) 237, 498, 732
Eam1104I CTCTTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 685
Eco57I CTGAAG 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 685
FaeI CATG 5 cut(s) 238, 338, 457, 710, 721
FatI CATG 5 cut(s) 234, 334, 453, 706, 717
Fnu4HI GCNGC 1 cut(s) 168
FokI GGATG 2 cut(s) 275, 366
Fsp4HI GCNGC 1 cut(s) 168
FspBI CTAG 1 cut(s) 624
GluI GCNGC 1 cut(s) 168
HaeIII GGCC 1 cut(s) 377
Hin1II CATG 5 cut(s) 238, 338, 457, 710, 721
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 4 cut(s) 307, 631, 677, 714
HphI GGTGA 3 cut(s) 125, 194, 475
Hpy166II GTNNAC 2 cut(s) 226, 408
Hpy188I TCNGA 3 cut(s) 207, 312, 682
Hpy188III TCNNGA 6 cut(s) 441, 454, 502, 635, 718, 736
Hpy8I GTNNAC 2 cut(s) 226, 408
HpyAV CCTTC 3 cut(s) 350, 413, 475
HpyCH4III ACNGT 2 cut(s) 182, 604
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 3 cut(s) 151, 386, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpySE526I ACGT 1 cut(s) 362
Hsp92II CATG 5 cut(s) 238, 338, 457, 710, 721
Kzo9I GATC 3 cut(s) 237, 498, 732
LmnI GCTCC 1 cut(s) 300
LpnPI CCDG 8 cut(s) 210, 396, 454, 590, 620, 672, 699, 749
Lsp1109I GCAGC 1 cut(s) 154
MaeI CTAG 1 cut(s) 624
MaeII ACGT 1 cut(s) 362
MalI GATC 3 cut(s) 239, 500, 734
MboI GATC 3 cut(s) 237, 498, 732
MboII GAAGA 5 cut(s) 148, 332, 490, 497, 702
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 1 cut(s) 732
MluCI AATT 8 cut(s) 6, 12, 18, 30, 82, 549, 642, 665
MlyI GAGTC 2 cut(s) 316, 708
MmeI TCCRAC 3 cut(s) 117, 290, 705
MseI TTAA 4 cut(s) 81, 108, 426, 435
MspCI CTTAAG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 687
MunI CAATTG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 687
MwoI GCNNNNNNNGC 1 cut(s) 383
NdeII GATC 3 cut(s) 237, 498, 732
NlaIII CATG 5 cut(s) 238, 338, 457, 710, 721
NlaIV GGNNCC 1 cut(s) 734
NspI RCATGY 1 cut(s) 338
PagI TCATGA 2 cut(s) 453, 717
PciI ACATGT 1 cut(s) 334
PfeI GAWTC 2 cut(s) 631, 677
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 2 cut(s) 315, 708
PpsI GAGTC 2 cut(s) 315, 708
PscI ACATGT 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 81
Psp1406I AACGTT 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 685
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 1 cut(s) 734
PspPI GGNCC 1 cut(s) 376
PsuI RGATCY 1 cut(s) 732
RsaI GTAC 3 cut(s) 41, 492, 622
RsaNI GTAC 3 cut(s) 40, 491, 621
SaqAI TTAA 4 cut(s) 81, 108, 426, 435
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 3 cut(s) 237, 498, 732
Sau96I GGNCC 1 cut(s) 376
ScaI AGTACT 2 cut(s) 41, 622
SchI GAGTC 2 cut(s) 316, 708
ScrFI CCNGG 1 cut(s) 687
SmlI CTYRAG 2 cut(s) 107, 304
SmoI CTYRAG 2 cut(s) 107, 304
Sse9I AATT 8 cut(s) 6, 12, 18, 30, 82, 549, 642, 665
SspMI CTAG 1 cut(s) 624
StyD4I CCNGG 1 cut(s) 685
TaaI ACNGT 2 cut(s) 182, 604
TaiI ACGT 1 cut(s) 365
TaqI TCGA 2 cut(s) 99, 295
TasI AATT 8 cut(s) 6, 12, 18, 30, 82, 549, 642, 665
TatI WGTACW 2 cut(s) 39, 620
TfiI GAWTC 2 cut(s) 631, 677
Tru1I TTAA 4 cut(s) 81, 108, 426, 435
Tru9I TTAA 4 cut(s) 81, 108, 426, 435
TseI GCWGC 1 cut(s) 167
TspDTI ATGAA 3 cut(s) 279, 470, 734
Vha464I CTTAAG 1 cut(s) 107
VspI ATTAAT 1 cut(s) 81
XapI RAATTY 1 cut(s) 665
XceI RCATGY 1 cut(s) 338
XcmI CCANNNNNNNNNTGG 1 cut(s) 399
XspI CTAG 1 cut(s) 624
ZrmI AGTACT 2 cut(s) 41, 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.