MD11G1088400.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005548
Physical Location & Seq
Forward (+)
7369132 .. 7370446
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1088400.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGAATGAGGTGATAGAGGGTTTGAAGAGGGTAGAAGTACGAATGATTGGGATTTGTGGAATGGGGGGCGTGGGTAAAACCACAATGGTAAAAGAAATCATCACAAGATTGGCAAAACTGAACCTGTTTGATAAGATTGTAATGGCAACTGTGTCTCAAAGTCCAAGTATTAGGACGATCCAGTCGGAAATTGCAGACGAAATAGGCTTGGAATTTAAGGTGGAATCCGAGCCCGGAAGAGCACGAAAGCTACATGAGAGACTCATGGAAATAAAGAGGATCCTCATTGTATTAGATGATGTATGGACAGAGCCTAATTTTGAGGCTATAGGGCTTCCTTATGGAGATACTCATAAAGGGTGCAAAATTTTGTTGACATCAAGAGATTCGGAAGTCTGCAACAGGATGGGAAGTCAACAAATTATTGCAGTCCCGACTTTAACAACAGAAGAATCACAGGAGCTCTTTCGAGAAATGGTAGGTGAATCCTTCAATGATCCTGATTTACGTTCCACTGCAGAAGATGTCGTGAAGGAATGTGGAGGTTTACCTATTGCTATTGTAACTGTTGGAAAAGCCCTAGAAAAGAAAAACAAGCGTGAATGGGATGATGCCCTTAATCAGCTGCGTAATTCCAGGCCAGAGAATATCCCTGGAGTGAACGACAAAGTTTATTCTTGCATAAAATGGAGTTATGATAGATTAGAGAGTGATGAAGCCAGGTCATGTCTTTTACTTTGTTGTTTATTTCCAGAAGATTATGATATTTCAATTGAGTATTTGGTTCGATATGGGTGGGGTCGAGGATATTTTAGCAGCATCGTTACTTTGGAAGATGCAAGAAATAGAGTGCATTCTTTGGTTTACCAACTAAAAAGAAGGTTTTTGTTGCTAGATAGTTATGTGAGTGAGGCTACGAAAATGCACGACGTAGTTCGTGATGTTGCCATATCAATTGCTTCAAGAGATCCACATGGATTTCTGATAAGTAATGCTGAAAATAAAAGGTGGCCAAATTTAGCTACATATGACCATTACACTACAATCTCACTTGTTGGCAAATTGGAGATTCCAGTTGGTTTGAAATGCCCAAAACTTGAGCTTCTACAAAAGATGAAAGGACGTTTTTCAGAAGGTAGCATGGACATCATTAGTGATACGATGAGAGAACTGAAGGTTTTAGCTTTGGTGGAAATGAAAGACCTAGGCTCATCAAGACCACTAGGAGTACTGAAGAACCTGCGAACCTTGTGCCTAGATAGAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

423

Amino Acids

47.81

Weight (kDa)

6.18

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 162 3.6e-36 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
Acc36I ACCTGC 1 cut(s) 1248
AclWI GGATC 5 cut(s) 172, 274, 287, 491, 962
AcoI YGGCCR 1 cut(s) 1010
AcsI RAATTY 4 cut(s) 212, 366, 1015, 1263
AcuI CTGAAG 2 cut(s) 1193, 1253
AfaI GTAC 2 cut(s) 39, 1230
AgsI TTSAA 5 cut(s) 25, 493, 771, 963, 1084
AjnI CCWGG 3 cut(s) 635, 652, 719
AluBI AGCT 6 cut(s) 250, 463, 625, 1022, 1102, 1184
AluI AGCT 6 cut(s) 250, 463, 625, 1022, 1102, 1184
Alw21I GWGCWC 2 cut(s) 244, 465
Alw26I GTCTC 2 cut(s) 159, 253
AlwI GGATC 5 cut(s) 172, 274, 287, 491, 962
AoxI GGCC 2 cut(s) 638, 1010
ApeKI GCWGC 2 cut(s) 625, 816
ApoI RAATTY 4 cut(s) 212, 366, 1015, 1263
AspA2I CCTAGG 1 cut(s) 1204
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 2 cut(s) 22, 494
AvrII CCTAGG 1 cut(s) 1204
BalI TGGCCA 1 cut(s) 1012
BamHI GGATCC 1 cut(s) 279
BanII GRGCYC 2 cut(s) 234, 465
Bbv12I GWGCWC 2 cut(s) 244, 465
BbvI GCAGC 2 cut(s) 612, 828
BccI CCATC 1 cut(s) 400
BcgI CGANNNNNNTGC 2 cut(s) 1233, 1267
BciT130I CCWGG 3 cut(s) 637, 654, 721
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 159, 253
BfaI CTAG 5 cut(s) 581, 893, 1205, 1223, 1256
BfmI CTRYAG 2 cut(s) 327, 516
BfuAI ACCTGC 1 cut(s) 1248
BisI GCNGC 2 cut(s) 626, 817
BlnI CCTAGG 1 cut(s) 1204
BlsI GCNGC 2 cut(s) 627, 818
BmcAI AGTACT 1 cut(s) 1230
Bme1390I CCNGG 4 cut(s) 234, 637, 654, 721
BmiI GGNNCC 1 cut(s) 281
BmrFI CCNGG 4 cut(s) 234, 637, 654, 721
BmsI GCATC 3 cut(s) 601, 826, 828
BpmI CTGGAG 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 1118
BpuMI CCSGG 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 652, 1204
Bse1I ACTGG 2 cut(s) 181, 1073
BseBI CCWGG 3 cut(s) 637, 654, 721
BseDI CCNNGG 2 cut(s) 652, 1204
BseGI GGATG 2 cut(s) 411, 613
BseNI ACTGG 2 cut(s) 181, 1073
BseXI GCAGC 2 cut(s) 612, 828
BshFI GGCC 2 cut(s) 640, 1012
BsiHKAI GWGCWC 2 cut(s) 244, 465
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 416
BsmAI GTCTC 2 cut(s) 159, 253
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 2 cut(s) 640, 1012
Bsp1286I GDGCHC 3 cut(s) 234, 244, 465
Bsp143I GATC 4 cut(s) 177, 279, 496, 967
BspANI GGCC 2 cut(s) 640, 1012
BspLI GGNNCC 1 cut(s) 281
BspMAI CTGCAG 1 cut(s) 520
BspMI ACCTGC 1 cut(s) 1248
BspPI GGATC 5 cut(s) 172, 274, 287, 491, 962
BspQI GCTCTTC 1 cut(s) 232
BsrI ACTGG 2 cut(s) 181, 1073
BssECI CCNNGG 2 cut(s) 652, 1204
BssMI GATC 4 cut(s) 177, 279, 496, 967
BssT1I CCWWGG 1 cut(s) 1204
Bst2UI CCWGG 3 cut(s) 637, 654, 721
Bst4CI ACNGT 2 cut(s) 151, 568
Bst6I CTCTTC 2 cut(s) 20, 232
BstF5I GGATG 2 cut(s) 411, 613
BstKTI GATC 4 cut(s) 180, 282, 499, 970
BstMAI GTCTC 2 cut(s) 159, 253
BstMBI GATC 4 cut(s) 177, 279, 496, 967
BstNI CCWGG 3 cut(s) 637, 654, 721
BstSCI CCNGG 4 cut(s) 232, 635, 652, 719
BstSFI CTRYAG 2 cut(s) 327, 516
BstV1I GCAGC 2 cut(s) 612, 828
BstX2I RGATCY 2 cut(s) 279, 967
BstYI RGATCY 2 cut(s) 279, 967
BsuRI GGCC 2 cut(s) 640, 1012
BtsCI GGATG 2 cut(s) 411, 613
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 513
BveI ACCTGC 1 cut(s) 1248
Csp6I GTAC 2 cut(s) 38, 1229
CviAII CATG 5 cut(s) 254, 265, 726, 974, 1141
CviQI GTAC 2 cut(s) 38, 1229
DpnI GATC 4 cut(s) 179, 281, 498, 969
DpnII GATC 4 cut(s) 177, 279, 496, 967
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 1010
Eam1104I CTCTTC 2 cut(s) 20, 232
EarI CTCTTC 2 cut(s) 20, 232
Ecl136II GAGCTC 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 1204
Eco24I GRGCYC 2 cut(s) 234, 465
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 2 cut(s) 1193, 1253
EcoICRI GAGCTC 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 635, 652, 719
EcoT14I CCWWGG 1 cut(s) 1204
EcoT38I GRGCYC 2 cut(s) 234, 465
ErhI CCWWGG 1 cut(s) 1204
FaeI CATG 5 cut(s) 257, 268, 729, 977, 1144
FaqI GGGAC 1 cut(s) 416
FatI CATG 5 cut(s) 253, 264, 725, 973, 1140
FauNDI CATATG 1 cut(s) 1027
Fnu4HI GCNGC 2 cut(s) 626, 817
FokI GGATG 2 cut(s) 418, 620
FriOI GRGCYC 2 cut(s) 234, 465
Fsp4HI GCNGC 2 cut(s) 626, 817
FspBI CTAG 5 cut(s) 581, 893, 1205, 1223, 1256
GluI GCNGC 2 cut(s) 626, 817
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 2 cut(s) 640, 1012
HapII CCGG 1 cut(s) 234
Hin1II CATG 5 cut(s) 257, 268, 729, 977, 1144
HincII GTYRAC 2 cut(s) 375, 416
HindII GTYRAC 2 cut(s) 375, 416
HinfI GANTC 6 cut(s) 224, 261, 386, 452, 485, 1069
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 22, 494
Hpy166II GTNNAC 5 cut(s) 375, 416, 548, 661, 865
Hpy188I TCNGA 5 cut(s) 187, 229, 391, 984, 1132
Hpy188III TCNNGA 9 cut(s) 381, 433, 470, 500, 529, 752, 938, 963, 1215
Hpy8I GTNNAC 5 cut(s) 375, 416, 548, 661, 865
Hpy99I CGWCG 1 cut(s) 932
HpyAV CCTTC 5 cut(s) 499, 526, 873, 1127, 1168
HpyCH4III ACNGT 2 cut(s) 151, 568
HpyCH4IV ACGT 3 cut(s) 508, 930, 1123
HpyCH4V TGCA 9 cut(s) 194, 363, 399, 428, 518, 681, 839, 853, 925
HpySE526I ACGT 3 cut(s) 508, 930, 1123
Hsp92II CATG 5 cut(s) 257, 268, 729, 977, 1144
Kzo9I GATC 4 cut(s) 177, 279, 496, 967
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 1 cut(s) 460
Lsp1109I GCAGC 2 cut(s) 612, 828
LweI GCATC 3 cut(s) 601, 826, 828
MaeI CTAG 5 cut(s) 581, 893, 1205, 1223, 1256
MaeII ACGT 3 cut(s) 508, 930, 1123
MaeIII GTNAC 2 cut(s) 562, 823
MalI GATC 4 cut(s) 179, 281, 498, 969
MboI GATC 4 cut(s) 177, 279, 496, 967
MboII GAAGA 7 cut(s) 37, 249, 461, 533, 767, 845, 1246
MfeI CAATTG 2 cut(s) 771, 954
MflI RGATCY 2 cut(s) 279, 967
MhlI GDGCHC 3 cut(s) 234, 244, 465
MlsI TGGCCA 1 cut(s) 1012
MluNI TGGCCA 1 cut(s) 1012
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 2 cut(s) 165, 550
MnlI CCTC 8 cut(s) 10, 21, 270, 293, 316, 536, 797, 904
Mox20I TGGCCA 1 cut(s) 1012
MscI TGGCCA 1 cut(s) 1012
MseI TTAA 3 cut(s) 216, 440, 618
Msp20I TGGCCA 1 cut(s) 1012
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 4 cut(s) 234, 637, 654, 721
MunI CAATTG 2 cut(s) 771, 954
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 3 cut(s) 637, 654, 721
NciI CCSGG 1 cut(s) 234
NdeI CATATG 1 cut(s) 1027
NdeII GATC 4 cut(s) 177, 279, 496, 967
NlaIII CATG 5 cut(s) 257, 268, 729, 977, 1144
NlaIV GGNNCC 1 cut(s) 281
PciSI GCTCTTC 1 cut(s) 232
PctI GAATGC 1 cut(s) 853
PfeI GAWTC 5 cut(s) 224, 386, 452, 485, 1069
PkrI GCNGC 2 cut(s) 627, 818
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 465
Psp6I CCWGG 3 cut(s) 635, 652, 719
PspGI CCWGG 3 cut(s) 635, 652, 719
PspN4I GGNNCC 1 cut(s) 281
PstI CTGCAG 1 cut(s) 520
PsuI RGATCY 2 cut(s) 279, 967
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 2 cut(s) 39, 1230
RsaNI GTAC 2 cut(s) 38, 1229
SacI GAGCTC 1 cut(s) 465
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 3 cut(s) 216, 440, 618
SatI GCNGC 2 cut(s) 626, 817
Sau3AI GATC 4 cut(s) 177, 279, 496, 967
ScaI AGTACT 1 cut(s) 1230
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 4 cut(s) 234, 637, 654, 721
SduI GDGCHC 3 cut(s) 234, 244, 465
SfaNI GCATC 3 cut(s) 601, 826, 828
SfcI CTRYAG 2 cut(s) 327, 516
SmlI CTYRAG 1 cut(s) 1097
SmoI CTYRAG 1 cut(s) 1097
SspMI CTAG 5 cut(s) 581, 893, 1205, 1223, 1256
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 4 cut(s) 232, 635, 652, 719
StyI CCWWGG 1 cut(s) 1204
TaaI ACNGT 2 cut(s) 151, 568
TaiI ACGT 3 cut(s) 511, 933, 1126
TaqI TCGA 3 cut(s) 469, 787, 802
TatI WGTACW 1 cut(s) 1228
TfiI GAWTC 5 cut(s) 224, 386, 452, 485, 1069
Tru1I TTAA 3 cut(s) 216, 440, 618
Tru9I TTAA 3 cut(s) 216, 440, 618
TscAI CASTG 1 cut(s) 520
TseI GCWGC 2 cut(s) 625, 816
TspDTI ATGAA 4 cut(s) 17, 729, 1130, 1211
TspRI CASTG 1 cut(s) 520
XapI RAATTY 4 cut(s) 212, 366, 1015, 1263
XmaJI CCTAGG 1 cut(s) 1204
XspI CTAG 5 cut(s) 581, 893, 1205, 1223, 1256
ZrmI AGTACT 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.