pycom11g07490

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005548
Physical Location & Seq
Forward (+)
5768847 .. 5770517
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g07490.2

Sequence Viewer

Length: 1545 bp
ATGCATGACATAGTTCGCGATGTTGCCATATCGATTGCTTCAAGAGATCGACATCGATTTCTGGTAAGAAGTCATGTTGAAAATAAAGAGTGGCCAAATTTAGCTACATATGACACAATCTCACTGGTTGGCGATCTGGAGATTCCAATTGGTTGCCCAAAACTTGAGCTTCTACAGACGATGAAAGGACGTTTTTCAAAAGGTAGCATGGACATCATTTGTAATGCGATGAAAGAACTAAAGGTTTTAGCTTTGGTGGAAATGGAAGACCTAGGCTCATCAAGATCACTAGGAGTACTGAAGAACCTGCGGACCTTGTGCCTAGATGGGTCTGACTTTGATGGCATGTCTACTGATGTTATTGGGAGTTTGGAGAATTTAGAAATCCTCAGCTTTCGGGATTGTTTTTACATGTGTGAGTTGCCGAGGGAAATTGGACGACTTAAACAGTTGAGGTTGTTAGATACGACAAACTGCTGGAATCTTAAGGTGATTCCACATGGTATCTTCTCCGGCTTATGTAGACTTGAAGAGTTGTATATGATGGGTAGCTTCGATAAATGGGACCTTGGAACAGGAAGAGAAGATAAGAGGATGGCAAGCATTTCTGAGGTGATGTCTCTGTCTCATCATTTGAAGGTTCTATCCATAAATGTCATCCACTTGTTGCCAAAAGACTTAGTCTTGAAAAGCCTAACAATAAAATTCCATATATTCTTGGGAACTGGGGAAAAGGGATTTTGTGAGATGATCGATAGACGTGCTGAAAGTGATGCAAGGAAGTTGATGATGATGACAGCTTATGCGTTCGAAAATTGTTTGGTGATTGATGAAAGTGATGCAAGCAAGTTGATGGAGAGTCAAGCAGTTAGACTTATATTGAAAAAATCTAAAAAATTGACTTTGCACAATGTTAAGAATTTCTCTGCCCTCACTGATTTAGACGAAGAGGGATTTCAAGATTTGAAAGTTTTGTATCTTGATAAGTGTCCAAATATCGAGTATCTTGTAAATGGAACAAGTGCACTCTTTCCTCGCAAACAATCAATTGGTCCGCTTCCGAATAGCTTTTTGACCAACCTAAGATTCCTTCAATTGTCAGGTTGTGGTGTCTTAAAATATGCCTTTTCACTATCAGCAGCGAAAAACTTGGTACAACTCCAGGAATTAGCTATTGGTGGATGTTATCAAATGGAAGAAATTGTGTCAAAGCAGGGGAGAGAACACGATGAGGAAGCCGATATGATATCTTTTCATAAATTAACCAATTTGAATCTCTTGGGTCTACAGAATTTGGTTGGTTTCTTCCAAGCTAACAAGCTATACTCCAACCAAGAGGAAACAACGCCAGGGGTTGAGCATCAATCTGCAGGAATATTTGAAAAAGCAATATTTCCATCAAAGTGCATTTCATGGTTTCCAAGTTTAGAAGAGGTAGAGTTGTCTGAAATGGAGTTTGAAGGCGTGCTATTTAAACACGATCGATCTGAAAATCCATATAGTTATGGATGGGCAGACAGCTCCAACATGTCCCCAGTTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

515

Amino Acids

58.12

Weight (kDa)

5.57

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 315
AccI GTMKAC 3 cut(s) 350, 523, 1285
AccII CGCG 1 cut(s) 18
AciI CCGC 2 cut(s) 310, 1055
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 5 cut(s) 97, 376, 704, 919, 1291
AcuI CTGAAG 1 cut(s) 320
AfaI GTAC 2 cut(s) 297, 1155
AflII CTTAAG 1 cut(s) 485
AflIII ACRYGT 2 cut(s) 411, 1527
AjiI CACGTC 1 cut(s) 761
AjnI CCWGG 2 cut(s) 1161, 1348
AjuI GAANNNNNNNTTGG 4 cut(s) 1070, 1102, 1158, 1190
Alw21I GWGCWC 1 cut(s) 1027
Alw26I GTCTC 2 cut(s) 624, 630
Alw44I GTGCAC 1 cut(s) 1023
AoxI GGCC 1 cut(s) 92
ApaLI GTGCAC 1 cut(s) 1023
ApeKI GCWGC 1 cut(s) 1139
ApoI RAATTY 5 cut(s) 97, 376, 704, 919, 1291
AspA2I CCTAGG 1 cut(s) 271
AspS9I GGNCC 3 cut(s) 312, 565, 1052
AsuHPI GGTGA 3 cut(s) 502, 625, 835
AsuII TTCGAA 1 cut(s) 810
AvaII GGWCC 3 cut(s) 312, 565, 1052
AvrII CCTAGG 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 1027
BalI TGGCCA 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 1027
BbvCI CCTCAGC 1 cut(s) 389
BbvI GCAGC 1 cut(s) 1151
BccI CCATC 7 cut(s) 320, 335, 538, 589, 847, 1405, 1503
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 2 cut(s) 1163, 1350
BcoDI GTCTC 2 cut(s) 624, 630
BfaI CTAG 3 cut(s) 272, 290, 323
BfmI CTRYAG 3 cut(s) 173, 1286, 1368
BfrI CTTAAG 1 cut(s) 485
BfuAI ACCTGC 1 cut(s) 315
BisI GCNGC 1 cut(s) 1140
BlnI CCTAGG 1 cut(s) 271
BlsI GCNGC 1 cut(s) 1141
BmcAI AGTACT 1 cut(s) 297
Bme1390I CCNGG 2 cut(s) 1163, 1350
Bme18I GGWCC 3 cut(s) 312, 565, 1052
BmgBI CACGTC 1 cut(s) 761
BmgT120I GGNCC 3 cut(s) 312, 565, 1052
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 2 cut(s) 1163, 1350
BmrI ACTGGG 2 cut(s) 735, 1529
BmsI GCATC 3 cut(s) 763, 829, 1369
BmuI ACTGGG 2 cut(s) 735, 1529
BpiI GAAGAC 1 cut(s) 273
BpmI CTGGAG 2 cut(s) 158, 1145
Bpu10I CCTNAGC 1 cut(s) 389
Bpu14I TTCGAA 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 185
Bsa29I ATCGAT 4 cut(s) 32, 55, 753, 1483
BsaAI YACGTR 1 cut(s) 1542
BsaBI GATNNNNATC 1 cut(s) 51
BsaJI CCNNGG 4 cut(s) 271, 425, 568, 1349
Bse1I ACTGG 3 cut(s) 129, 730, 1535
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 2 cut(s) 1163, 1350
BseCI ATCGAT 4 cut(s) 32, 55, 753, 1483
BseDI CCNNGG 4 cut(s) 271, 425, 568, 1349
BseGI GGATG 4 cut(s) 600, 657, 1187, 1514
BseJI GATNNNNATC 1 cut(s) 51
BseMII CTCAG 2 cut(s) 403, 600
BseNI ACTGG 3 cut(s) 129, 730, 1535
BseSI GKGCMC 1 cut(s) 1027
BseXI GCAGC 1 cut(s) 1151
Bsh1236I CGCG 1 cut(s) 18
Bsh1285I CGRYCG 1 cut(s) 1483
BshFI GGCC 1 cut(s) 94
BshVI ATCGAT 4 cut(s) 32, 55, 753, 1483
BsiEI CGRYCG 1 cut(s) 1483
BsiHKAI GWGCWC 1 cut(s) 1027
BsiSI CCGG 1 cut(s) 513
BslFI GGGAC 2 cut(s) 578, 1516
BsmAI GTCTC 2 cut(s) 624, 630
BsmFI GGGAC 2 cut(s) 578, 1516
BsnI GGCC 1 cut(s) 94
Bsp119I TTCGAA 1 cut(s) 810
Bsp1286I GDGCHC 1 cut(s) 1027
Bsp143I GATC 6 cut(s) 46, 133, 284, 750, 1480, 1484
Bsp68I TCGCGA 1 cut(s) 18
BspACI CCGC 2 cut(s) 310, 1055
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 2 cut(s) 402, 601
BspDI ATCGAT 4 cut(s) 32, 55, 753, 1483
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 566
BspMAI CTGCAG 1 cut(s) 1372
BspMI ACCTGC 1 cut(s) 315
BspT104I TTCGAA 1 cut(s) 810
BspTI CTTAAG 1 cut(s) 485
BsrI ACTGG 3 cut(s) 129, 730, 1535
BssECI CCNNGG 4 cut(s) 271, 425, 568, 1349
BssMI GATC 6 cut(s) 46, 133, 284, 750, 1480, 1484
BssT1I CCWWGG 2 cut(s) 271, 568
Bst2UI CCWGG 2 cut(s) 1163, 1350
Bst4CI ACNGT 1 cut(s) 450
Bst6I CTCTTC 4 cut(s) 525, 574, 942, 1425
BstAFI CTTAAG 1 cut(s) 485
BstBAI YACGTR 1 cut(s) 1542
BstBI TTCGAA 1 cut(s) 810
BstC8I GCNNGC 3 cut(s) 601, 844, 1466
BstDEI CTNAG 4 cut(s) 389, 609, 679, 1082
BstF5I GGATG 4 cut(s) 600, 657, 1187, 1514
BstFNI CGCG 1 cut(s) 18
BstKTI GATC 6 cut(s) 49, 136, 287, 753, 1483, 1487
BstMAI GTCTC 2 cut(s) 624, 630
BstMBI GATC 6 cut(s) 46, 133, 284, 750, 1480, 1484
BstMCI CGRYCG 1 cut(s) 1483
BstNI CCWGG 2 cut(s) 1163, 1350
BstNSI RCATGY 3 cut(s) 349, 415, 1531
BstSCI CCNGG 2 cut(s) 1161, 1348
BstSFI CTRYAG 3 cut(s) 173, 1286, 1368
BstSLI GKGCMC 1 cut(s) 1027
BstSNI TACGTA 1 cut(s) 1542
BstUI CGCG 1 cut(s) 18
BstV1I GCAGC 1 cut(s) 1151
BstV2I GAAGAC 1 cut(s) 273
Bsu15I ATCGAT 4 cut(s) 32, 55, 753, 1483
BsuRI GGCC 1 cut(s) 94
BsuTUI ATCGAT 4 cut(s) 32, 55, 753, 1483
BtgZI GCGATG 2 cut(s) 33, 242
BtrI CACGTC 1 cut(s) 761
BtsCI GGATG 4 cut(s) 600, 657, 1187, 1514
BtsIMutI CAGTG 2 cut(s) 122, 933
BtuMI TCGCGA 1 cut(s) 18
BveI ACCTGC 1 cut(s) 315
Cac8I GCNNGC 3 cut(s) 601, 844, 1466
Cfr13I GGNCC 3 cut(s) 312, 565, 1052
ClaI ATCGAT 4 cut(s) 32, 55, 753, 1483
Csp6I GTAC 2 cut(s) 296, 1154
CviAII CATG 8 cut(s) 5, 74, 208, 346, 412, 500, 1413, 1528
CviQI GTAC 2 cut(s) 296, 1154
DdeI CTNAG 4 cut(s) 389, 609, 679, 1082
DpnI GATC 6 cut(s) 48, 135, 286, 752, 1482, 1486
DpnII GATC 6 cut(s) 46, 133, 284, 750, 1480, 1484
DraI TTTAAA 1 cut(s) 1474
EaeI YGGCCR 1 cut(s) 92
Eam1104I CTCTTC 4 cut(s) 525, 574, 942, 1425
EarI CTCTTC 4 cut(s) 525, 574, 942, 1425
Eco105I TACGTA 1 cut(s) 1542
Eco130I CCWWGG 2 cut(s) 271, 568
Eco32I GATATC 1 cut(s) 1248
Eco47I GGWCC 3 cut(s) 312, 565, 1052
Eco57I CTGAAG 1 cut(s) 320
EcoO109I RGGNCCY 1 cut(s) 565
EcoRII CCWGG 2 cut(s) 1161, 1348
EcoRV GATATC 1 cut(s) 1248
EcoT14I CCWWGG 2 cut(s) 271, 568
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 271, 568
FaeI CATG 8 cut(s) 8, 77, 211, 349, 415, 503, 1416, 1531
FaqI GGGAC 2 cut(s) 578, 1516
FatI CATG 8 cut(s) 4, 73, 207, 345, 411, 499, 1412, 1527
FauNDI CATATG 1 cut(s) 109
FblI GTMKAC 3 cut(s) 350, 523, 1285
Fnu4HI GCNGC 1 cut(s) 1140
FokI GGATG 4 cut(s) 607, 644, 1194, 1521
Fsp4HI GCNGC 1 cut(s) 1140
FspBI CTAG 3 cut(s) 272, 290, 323
GluI GCNGC 1 cut(s) 1140
GsuI CTGGAG 2 cut(s) 158, 1145
HaeIII GGCC 1 cut(s) 94
HapII CCGG 1 cut(s) 513
Hin1II CATG 8 cut(s) 8, 77, 211, 349, 415, 503, 1416, 1531
HinfI GANTC 6 cut(s) 142, 481, 493, 859, 1086, 1273
HpaII CCGG 1 cut(s) 513
HphI GGTGA 3 cut(s) 502, 625, 835
Hpy166II GTNNAC 4 cut(s) 351, 524, 1025, 1286
Hpy188I TCNGA 5 cut(s) 334, 610, 1062, 1447, 1489
Hpy188III TCNNGA 8 cut(s) 17, 42, 137, 282, 398, 685, 959, 980
Hpy8I GTNNAC 4 cut(s) 351, 524, 1025, 1286
HpyAV CCTTC 3 cut(s) 631, 1100, 1454
HpyCH4III ACNGT 1 cut(s) 450
HpyCH4IV ACGT 3 cut(s) 190, 760, 1541
HpyCH4V TGCA 7 cut(s) 4, 776, 842, 907, 1025, 1370, 1407
HpyF3I CTNAG 4 cut(s) 389, 609, 679, 1082
HpySE526I ACGT 3 cut(s) 190, 760, 1541
Hsp92II CATG 8 cut(s) 8, 77, 211, 349, 415, 503, 1416, 1531
Kzo9I GATC 6 cut(s) 46, 133, 284, 750, 1480, 1484
LmnI GCTCC 1 cut(s) 1526
Lsp1109I GCAGC 1 cut(s) 1151
LweI GCATC 3 cut(s) 763, 829, 1369
MaeI CTAG 3 cut(s) 272, 290, 323
MaeII ACGT 3 cut(s) 190, 760, 1541
MaeIII GTNAC 1 cut(s) 1537
MalI GATC 6 cut(s) 48, 135, 286, 752, 1482, 1486
MboI GATC 6 cut(s) 46, 133, 284, 750, 1480, 1484
MfeI CAATTG 3 cut(s) 147, 1047, 1094
MhlI GDGCHC 1 cut(s) 1027
MlsI TGGCCA 1 cut(s) 94
MluNI TGGCCA 1 cut(s) 94
MlyI GAGTC 1 cut(s) 868
MmeI TCCRAC 1 cut(s) 1353
Mox20I TGGCCA 1 cut(s) 94
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 94
MseI TTAA 6 cut(s) 444, 486, 915, 1115, 1262, 1473
MslI CAYNNNNRTG 1 cut(s) 1402
Msp20I TGGCCA 1 cut(s) 94
MspCI CTTAAG 1 cut(s) 485
MspI CCGG 1 cut(s) 513
MspR9I CCNGG 2 cut(s) 1163, 1350
MunI CAATTG 3 cut(s) 147, 1047, 1094
MvaI CCWGG 2 cut(s) 1163, 1350
MvnI CGCG 1 cut(s) 18
NdeI CATATG 1 cut(s) 109
NdeII GATC 6 cut(s) 46, 133, 284, 750, 1480, 1484
NlaIII CATG 8 cut(s) 8, 77, 211, 349, 415, 503, 1416, 1531
NlaIV GGNNCC 1 cut(s) 566
NmeAIII GCCGAG 1 cut(s) 450
NruI TCGCGA 1 cut(s) 18
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 349, 415, 1531
NspV TTCGAA 1 cut(s) 810
PciI ACATGT 2 cut(s) 411, 1527
PfeI GAWTC 5 cut(s) 142, 481, 493, 1086, 1273
PflFI GACNNNGTC 1 cut(s) 680
PfoI TCCNGGA 1 cut(s) 1161
PkrI GCNGC 1 cut(s) 1141
Ple19I CGATCG 1 cut(s) 1483
PleI GAGTC 1 cut(s) 867
PpsI GAGTC 1 cut(s) 867
Ppu21I YACGTR 1 cut(s) 1542
PpuMI RGGWCCY 1 cut(s) 565
PscI ACATGT 2 cut(s) 411, 1527
Psp5II RGGWCCY 1 cut(s) 565
Psp6I CCWGG 2 cut(s) 1161, 1348
PspGI CCWGG 2 cut(s) 1161, 1348
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 3 cut(s) 312, 565, 1052
PspPPI RGGWCCY 1 cut(s) 565
PstI CTGCAG 1 cut(s) 1372
PsyI GACNNNGTC 1 cut(s) 680
PvuI CGATCG 1 cut(s) 1483
RruI TCGCGA 1 cut(s) 18
RsaI GTAC 2 cut(s) 297, 1155
RsaNI GTAC 2 cut(s) 296, 1154
RseI CAYNNNNRTG 1 cut(s) 1402
SaqAI TTAA 6 cut(s) 444, 486, 915, 1115, 1262, 1473
SatI GCNGC 1 cut(s) 1140
Sau3AI GATC 6 cut(s) 46, 133, 284, 750, 1480, 1484
Sau96I GGNCC 3 cut(s) 312, 565, 1052
ScaI AGTACT 1 cut(s) 297
SchI GAGTC 1 cut(s) 868
ScrFI CCNGG 2 cut(s) 1163, 1350
SduI GDGCHC 1 cut(s) 1027
SfaNI GCATC 3 cut(s) 763, 829, 1369
SfcI CTRYAG 3 cut(s) 173, 1286, 1368
SfuI TTCGAA 1 cut(s) 810
SinI GGWCC 3 cut(s) 312, 565, 1052
SmiMI CAYNNNNRTG 1 cut(s) 1402
SmlI CTYRAG 2 cut(s) 164, 485
SmoI CTYRAG 2 cut(s) 164, 485
SnaBI TACGTA 1 cut(s) 1542
SsiI CCGC 2 cut(s) 310, 1055
SspI AATATT 2 cut(s) 1377, 1392
SspMI CTAG 3 cut(s) 272, 290, 323
StyD4I CCNGG 2 cut(s) 1161, 1348
StyI CCWWGG 2 cut(s) 271, 568
TaaI ACNGT 1 cut(s) 450
TaiI ACGT 3 cut(s) 193, 763, 1544
TaqI TCGA 8 cut(s) 32, 49, 55, 555, 753, 810, 999, 1483
TatI WGTACW 1 cut(s) 295
TfiI GAWTC 5 cut(s) 142, 481, 493, 1086, 1273
Tru1I TTAA 6 cut(s) 444, 486, 915, 1115, 1262, 1473
Tru9I TTAA 6 cut(s) 444, 486, 915, 1115, 1262, 1473
TscAI CASTG 2 cut(s) 129, 940
TseI GCWGC 1 cut(s) 1139
TspDTI ATGAA 5 cut(s) 197, 245, 846, 1244, 1401
TspRI CASTG 2 cut(s) 129, 940
Tth111I GACNNNGTC 1 cut(s) 680
Vha464I CTTAAG 1 cut(s) 485
VneI GTGCAC 1 cut(s) 1023
VpaK11BI GGWCC 3 cut(s) 312, 565, 1052
XapI RAATTY 5 cut(s) 97, 376, 704, 919, 1291
XceI RCATGY 3 cut(s) 349, 415, 1531
XmaJI CCTAGG 1 cut(s) 271
XmiI GTMKAC 3 cut(s) 350, 523, 1285
XspI CTAG 3 cut(s) 272, 290, 323
ZrmI AGTACT 1 cut(s) 297
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.