MD11G1086000.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005548
Physical Location & Seq
Forward (+)
7256905 .. 7259218
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1086000.v1.1.491

Sequence Viewer

Length: 2238 bp
ATGGAAATTATAATTGCAATTGCCTCAAAAATTGGAGAGTCATTGGTCGCACCAATAGGAACAGAGTCTGCCTATTTGATTAATTACCATTCCAACCTAGAAAATCTTAAGCGTGAGATAAAAAAGCTCTGTGACAAGAAAAATGAAGTGCAGGGATGGGTAAATGCTGCCAAAAGGAACGGTAAATTTATCCACCCTGATATTCAGAGTTGGCTAAAGAATGTGGACGAAATGATCCAAAAAGTGTCGCATTTTGAGGTTGAGATTGACAGGAAAAGGCGATGTATGTATCGATGGAGCTTGAGTCGGAAAGCCTATAAGATTACACAACATGTTCTCCAGCTCCAAAACGAAGGAACATTTGAAAATGTGGCCTATGCTGCACCTTCACGAGAGATATGGTCAACATTCAAAGACGGATTTAAACATTTCAAGTCCAGAATGGAAAACATGAATGAGGTGATAGAGGGTTTGAAGAGGGAAGAAGTAAGAATGATCGGGATTTGTGGAATGGGGGGTGTGGGTAAAACCACAATGGCGAAAGAAATCATCAAAAGATTAGCAGAACGGAACATGTTTGATAAAACTGTAATGGCAACTGTGTCTCAAAGCCCAAGTATTAAGACGATCCAGTTGGACATTGCAGCACAAATAGATTTGAAATTTGATGAGGATTCTGAGTCCGGAAGAGCACTAAAGCTGCATGACAGACTCATGGAAATAAGGAGGATACTGATTGTGTTAGATGATGTATGGACAGAGCTTAATTTTGAGGCTATAGGACTTCCTTATGGACATACTCATAAAGGGTGCAAAATTTTGTTGACATCACGGAATTTGGAAGTCTGCAATGCGATGGAAAGTCAACAAATTATTGCAGTCCCAACTTTAACAACAGAAGAATCACGGGAGCTCTTTAGAGAAATCGTAGGTGAATCCTTCGATGATCCAGATTTCCATTCCATTGCAGAAGAGGTCGTGAATGAATGTGGAGGTTTACCTATTGCAATTGTAACTGTTGGAAAAGCCCTAGAAAAGAAAAAGAAGCACGAATGGGTTAATGCCCTTAATCAGCTGCGAAAGTCTATCCCAGATAACATCCCTGGACTAAACGGCAAAGTTTATTCAAGCATAAAATGGAGTTATGATAGATTGGAGAGTGATGAAGCCAAGTCATGTCTTTTACTTTGTTGTTTATTTCCAGAAGATTATAGCATTCCAATTGAGGATTTGGTTCGATATGGGTGGGATCGAGGATATTTTAGCAGCAGCGATACTTTGGAAGAAGCAAGAAATAAAGTGCATTCTTTGGTTGACCAACTACAAAGAAGGTTTTTGTTACTAGATAATGACTGGAGTGGGACTACAAAAATGCATGACATAGTTCGCGATGTTGTCATATCAATTGCTTCAAGAGATCCACATGGATTTCTGATAAGAAGTAATGCTGAAAATAAAGGGTGGCCAAATCTAGCTACATATGACCATTACACTACAATCTCACTTGTTGGCGATTTGGAGATTCAAGATGGTTTGAAATGCCCAAAACTTGAGTTTCTACAGACGATGAAAGGACGTTTTTCAGAAGGTAGCATGGACAACATTTGTAAGGCGATGAAAGAACTGAAGGTTTTAGCTTTGGTGCAAATGGAAATGGAAGACCTAGGCTCATCAAGCTCACTAGGATTACTGAAGAACCTGCGGACCTTGACCCTAAATGGGTCTAAATTTGATGGCATGTCTACTGATGTTATTGGGAGTTTGGAGAATTTAGAAATCCTCAGCTTTCGGGATTGTGATTCCATGCGTGAGTTGCCGAGGGAAATTGGACGACTTAAACAGTTAAGGTTGTTAGATACGACAAATTGCAGGAATCTTGAGGTGATTCCACATGGTATCTTCTCCAGCTTATGTAGACTTGAAGAGTTATATATGCTTGATAGCTTTAATAAATGGGAAATTGAAACAGGAAGAGAAGAGAAGGGGATGGCAAGCATTTCTAAGGTGATGTCTCTGTCTCATCATTTGAAGGTTCTATCCATAAATATACCCCCCAATTTCATCCACTTGTTGCCAAAAGACTTAGTCTTGAAAAGCCCAAGATTCGCTATACGGCTTACACCAGCAAGGTATTGTTTCCCCACAACTTATGCATTCGAAAATTGTTTGGCAATTGATGAAAGTGATGCGAGGGAACTGATGGAGAGTCAAGCAACCAAGAGGGATTTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

746

Amino Acids

85.09

Weight (kDa)

6.2

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 149 - 312 2.7e-36 NB-ARC domain
LRR_14 PF23598 539 - 648 1.2e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 1707
AccI GTMKAC 2 cut(s) 1742, 1915
AccII CGCG 1 cut(s) 1389
AccIII TCCGGA 1 cut(s) 683
AciI CCGC 1 cut(s) 1702
AclWI GGATC 5 cut(s) 229, 622, 941, 1257, 1412
AcoI YGGCCR 1 cut(s) 1463
AcsI RAATTY 6 cut(s) 185, 662, 816, 835, 1727, 1768
AcuI CTGAAG 2 cut(s) 1646, 1712
AfiI CCNNNNNNNGG 1 cut(s) 1719
AflII CTTAAG 1 cut(s) 107
AflIII ACRYGT 2 cut(s) 331, 573
AjnI CCWGG 1 cut(s) 1102
AjuI GAANNNNNNNTTGG 1 cut(s) 2210
Alw21I GWGCWC 2 cut(s) 694, 915
Alw26I GTCTC 3 cut(s) 609, 2016, 2022
AlwI GGATC 5 cut(s) 229, 622, 941, 1257, 1412
AlwNI CAGNNNCTG 1 cut(s) 68
Aor13HI TCCGGA 1 cut(s) 683
AoxI GGCC 2 cut(s) 372, 1463
ApeKI GCWGC 7 cut(s) 167, 380, 644, 700, 1075, 1266, 1269
ApoI RAATTY 6 cut(s) 185, 662, 816, 835, 1727, 1768
AseI ATTAAT 1 cut(s) 81
AspA2I CCTAGG 1 cut(s) 1663
AspS9I GGNCC 1 cut(s) 1704
AsuHPI GGTGA 4 cut(s) 472, 944, 1894, 2017
AsuII TTCGAA 1 cut(s) 2157
AvaII GGWCC 1 cut(s) 1704
AvrII CCTAGG 1 cut(s) 1663
BalI TGGCCA 1 cut(s) 1465
BanII GRGCYC 1 cut(s) 915
BauI CACGAG 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 1665
Bbv12I GWGCWC 2 cut(s) 694, 915
BbvCI CCTCAGC 1 cut(s) 1781
BbvI GCAGC 7 cut(s) 154, 367, 656, 687, 1062, 1278, 1281
BccI CCATC 7 cut(s) 150, 288, 850, 1523, 1727, 1981, 2194
BceAI ACGGC 2 cut(s) 1129, 2129
BciT130I CCWGG 1 cut(s) 1104
BciVI GTATCC 1 cut(s) 723
BcoDI GTCTC 3 cut(s) 609, 2016, 2022
BfaI CTAG 6 cut(s) 98, 1031, 1343, 1472, 1664, 1682
BfmI CTRYAG 2 cut(s) 777, 1559
BfrI CTTAAG 1 cut(s) 107
BfuAI ACCTGC 1 cut(s) 1707
BfuI GTATCC 1 cut(s) 723
BisI GCNGC 7 cut(s) 168, 381, 645, 701, 1076, 1267, 1270
BlnI CCTAGG 1 cut(s) 1663
BlsI GCNGC 7 cut(s) 169, 382, 646, 702, 1077, 1268, 1271
Bme1390I CCNGG 1 cut(s) 1104
Bme18I GGWCC 1 cut(s) 1704
BmgT120I GGNCC 1 cut(s) 1704
BmrFI CCNGG 1 cut(s) 1104
BmsI GCATC 1 cut(s) 2176
BpiI GAAGAC 1 cut(s) 1665
BpmI CTGGAG 3 cut(s) 323, 1375, 1888
Bpu10I CCTNAGC 1 cut(s) 1781
Bpu14I TTCGAA 1 cut(s) 2157
BpuEI CTTGAG 3 cut(s) 322, 1571, 1898
Bsa29I ATCGAT 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 1102, 1663, 1817
BsaWI WCCGGW 1 cut(s) 683
BsaXI ACNNNNNCTCC 4 cut(s) 289, 319, 321, 351
Bsc4I CCNNNNNNNGG 1 cut(s) 1719
Bse1I ACTGG 2 cut(s) 631, 1358
Bse3DI GCAATG 3 cut(s) 639, 856, 963
BseAI TCCGGA 1 cut(s) 683
BseBI CCWGG 1 cut(s) 1104
BseCI ATCGAT 1 cut(s) 292
BseDI CCNNGG 3 cut(s) 1102, 1663, 1817
BseGI GGATG 4 cut(s) 161, 1098, 1992, 2061
BseLI CCNNNNNNNGG 1 cut(s) 1719
BseMI GCAATG 3 cut(s) 639, 856, 963
BseMII CTCAG 2 cut(s) 669, 1795
BseNI ACTGG 2 cut(s) 631, 1358
BseXI GCAGC 7 cut(s) 154, 367, 656, 687, 1062, 1278, 1281
BsgI GTGCAG 2 cut(s) 170, 366
Bsh1236I CGCG 1 cut(s) 1389
BshFI GGCC 2 cut(s) 374, 1465
BshVI ATCGAT 1 cut(s) 292
BsiHKAI GWGCWC 2 cut(s) 694, 915
BsiSI CCGG 1 cut(s) 684
BslFI GGGAC 2 cut(s) 866, 1375
BslI CCNNNNNNNGG 1 cut(s) 1719
BsmAI GTCTC 3 cut(s) 609, 2016, 2022
BsmFI GGGAC 2 cut(s) 866, 1375
BsmI GAATGC 3 cut(s) 1215, 1303, 2153
BsnI GGCC 2 cut(s) 374, 1465
Bsp119I TTCGAA 1 cut(s) 2157
Bsp1286I GDGCHC 2 cut(s) 694, 915
Bsp13I TCCGGA 1 cut(s) 683
Bsp143I GATC 6 cut(s) 234, 495, 627, 946, 1249, 1417
Bsp68I TCGCGA 1 cut(s) 1389
BspACI CCGC 1 cut(s) 1702
BspANI GGCC 2 cut(s) 374, 1465
BspCNI CTCAG 2 cut(s) 670, 1794
BspDI ATCGAT 1 cut(s) 292
BspEI TCCGGA 1 cut(s) 683
BspFNI CGCG 1 cut(s) 1389
BspMI ACCTGC 1 cut(s) 1707
BspPI GGATC 5 cut(s) 229, 622, 941, 1257, 1412
BspQI GCTCTTC 1 cut(s) 682
BspT104I TTCGAA 1 cut(s) 2157
BspTI CTTAAG 1 cut(s) 107
BsrDI GCAATG 3 cut(s) 639, 856, 963
BsrI ACTGG 2 cut(s) 631, 1358
BssECI CCNNGG 3 cut(s) 1102, 1663, 1817
BssMI GATC 6 cut(s) 234, 495, 627, 946, 1249, 1417
BssSI CACGAG 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 1663
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 1104
Bst4CI ACNGT 5 cut(s) 182, 589, 601, 1018, 1842
Bst6I CTCTTC 6 cut(s) 470, 682, 966, 1917, 1966, 1971
BstAFI CTTAAG 1 cut(s) 107
BstBI TTCGAA 1 cut(s) 2157
BstC8I GCNNGC 1 cut(s) 1993
BstDEI CTNAG 4 cut(s) 678, 1781, 2001, 2083
BstF5I GGATG 4 cut(s) 161, 1098, 1992, 2061
BstFNI CGCG 1 cut(s) 1389
BstKTI GATC 6 cut(s) 237, 498, 630, 949, 1252, 1420
BstMAI GTCTC 3 cut(s) 609, 2016, 2022
BstMBI GATC 6 cut(s) 234, 495, 627, 946, 1249, 1417
BstMWI GCNNNNNNNGC 3 cut(s) 380, 1674, 1813
BstNI CCWGG 1 cut(s) 1104
BstNSI RCATGY 3 cut(s) 335, 577, 1741
BstSCI CCNGG 1 cut(s) 1102
BstSFI CTRYAG 2 cut(s) 777, 1559
BstUI CGCG 1 cut(s) 1389
BstV1I GCAGC 7 cut(s) 154, 367, 656, 687, 1062, 1278, 1281
BstV2I GAAGAC 1 cut(s) 1665
BstX2I RGATCY 1 cut(s) 1417
BstYI RGATCY 1 cut(s) 1417
Bsu15I ATCGAT 1 cut(s) 292
BsuI GTATCC 1 cut(s) 723
BsuRI GGCC 2 cut(s) 374, 1465
BsuTUI ATCGAT 1 cut(s) 292
BtgZI GCGATG 4 cut(s) 295, 869, 1404, 1628
BtsCI GGATG 4 cut(s) 161, 1098, 1992, 2061
BtuMI TCGCGA 1 cut(s) 1389
BveI ACCTGC 1 cut(s) 1707
Cac8I GCNNGC 1 cut(s) 1993
CaiI CAGNNNCTG 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 1704
ClaI ATCGAT 1 cut(s) 292
DdeI CTNAG 4 cut(s) 678, 1781, 2001, 2083
DpnI GATC 6 cut(s) 236, 497, 629, 948, 1251, 1419
DpnII GATC 6 cut(s) 234, 495, 627, 946, 1249, 1417
DraI TTTAAA 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 1463
Eam1104I CTCTTC 6 cut(s) 470, 682, 966, 1917, 1966, 1971
EarI CTCTTC 6 cut(s) 470, 682, 966, 1917, 1966, 1971
Ecl136II GAGCTC 1 cut(s) 913
Eco130I CCWWGG 1 cut(s) 1663
Eco24I GRGCYC 1 cut(s) 915
Eco47I GGWCC 1 cut(s) 1704
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 2 cut(s) 1646, 1712
EcoICRI GAGCTC 1 cut(s) 913
EcoRII CCWGG 1 cut(s) 1102
EcoT14I CCWWGG 1 cut(s) 1663
EcoT22I ATGCAT 2 cut(s) 1377, 2155
EcoT38I GRGCYC 1 cut(s) 915
ErhI CCWWGG 1 cut(s) 1663
FaqI GGGAC 2 cut(s) 866, 1375
FauNDI CATATG 1 cut(s) 1480
FblI GTMKAC 2 cut(s) 1742, 1915
Fnu4HI GCNGC 7 cut(s) 168, 381, 645, 701, 1076, 1267, 1270
FokI GGATG 4 cut(s) 168, 1085, 1999, 2048
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 7 cut(s) 168, 381, 645, 701, 1076, 1267, 1270
FspBI CTAG 6 cut(s) 98, 1031, 1343, 1472, 1664, 1682
GluI GCNGC 7 cut(s) 168, 381, 645, 701, 1076, 1267, 1270
GsuI CTGGAG 3 cut(s) 323, 1375, 1888
HaeIII GGCC 2 cut(s) 374, 1465
HapII CCGG 1 cut(s) 684
HincII GTYRAC 4 cut(s) 405, 825, 866, 1315
HindII GTYRAC 4 cut(s) 405, 825, 866, 1315
HpaII CCGG 1 cut(s) 684
HphI GGTGA 4 cut(s) 472, 944, 1894, 2017
Hpy166II GTNNAC 8 cut(s) 226, 405, 825, 866, 998, 1315, 1743, 1916
Hpy188I TCNGA 5 cut(s) 207, 309, 679, 1434, 1585
Hpy8I GTNNAC 8 cut(s) 226, 405, 825, 866, 998, 1315, 1743, 1916
HpyAV CCTTC 8 cut(s) 347, 396, 949, 1323, 1580, 1621, 1975, 2023
HpyCH4III ACNGT 5 cut(s) 182, 589, 601, 1018, 1842
HpyCH4IV ACGT 1 cut(s) 1576
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 1674, 1813
HpyF3I CTNAG 4 cut(s) 678, 1781, 2001, 2083
HpySE526I ACGT 1 cut(s) 1576
Kpn2I TCCGGA 1 cut(s) 683
Kzo9I GATC 6 cut(s) 234, 495, 627, 946, 1249, 1417
LguI GCTCTTC 1 cut(s) 682
LmnI GCTCC 3 cut(s) 297, 348, 910
Lsp1109I GCAGC 7 cut(s) 154, 367, 656, 687, 1062, 1278, 1281
LweI GCATC 1 cut(s) 2176
MaeI CTAG 6 cut(s) 98, 1031, 1343, 1472, 1664, 1682
MaeII ACGT 1 cut(s) 1576
MaeIII GTNAC 3 cut(s) 131, 1012, 1338
MalI GATC 6 cut(s) 236, 497, 629, 948, 1251, 1419
MboI GATC 6 cut(s) 234, 495, 627, 946, 1249, 1417
MfeI CAATTG 5 cut(s) 18, 1008, 1221, 1404, 2172
MflI RGATCY 1 cut(s) 1417
MhlI GDGCHC 2 cut(s) 694, 915
MlsI TGGCCA 1 cut(s) 1465
MluNI TGGCCA 1 cut(s) 1465
MlyI GAGTC 6 cut(s) 47, 74, 313, 689, 705, 2215
MmeI TCCRAC 4 cut(s) 117, 287, 615, 1000
Mox20I TGGCCA 1 cut(s) 1465
Mph1103I ATGCAT 2 cut(s) 1377, 2155
MroI TCCGGA 1 cut(s) 683
MscI TGGCCA 1 cut(s) 1465
Msp20I TGGCCA 1 cut(s) 1465
MspA1I CMGCKG 1 cut(s) 1075
MspCI CTTAAG 1 cut(s) 107
MspI CCGG 1 cut(s) 684
MspR9I CCNGG 1 cut(s) 1104
MunI CAATTG 5 cut(s) 18, 1008, 1221, 1404, 2172
Mva1269I GAATGC 3 cut(s) 1215, 1303, 2153
MvaI CCWGG 1 cut(s) 1104
MvnI CGCG 1 cut(s) 1389
MwoI GCNNNNNNNGC 3 cut(s) 380, 1674, 1813
NdeI CATATG 1 cut(s) 1480
NdeII GATC 6 cut(s) 234, 495, 627, 946, 1249, 1417
NmeAIII GCCGAG 1 cut(s) 1842
NmuCI GTSAC 1 cut(s) 131
NruI TCGCGA 1 cut(s) 1389
NsiI ATGCAT 2 cut(s) 1377, 2155
NspI RCATGY 3 cut(s) 335, 577, 1741
NspV TTCGAA 1 cut(s) 2157
PciI ACATGT 2 cut(s) 331, 573
PciSI GCTCTTC 1 cut(s) 682
PctI GAATGC 3 cut(s) 1215, 1303, 2153
PfeI GAWTC 8 cut(s) 674, 902, 935, 1522, 1799, 1873, 1885, 2103
PflFI GACNNNGTC 1 cut(s) 2084
PkrI GCNGC 7 cut(s) 169, 382, 646, 702, 1077, 1268, 1271
PleI GAGTC 6 cut(s) 46, 73, 312, 688, 705, 2214
PpsI GAGTC 6 cut(s) 46, 73, 312, 688, 705, 2214
PscI ACATGT 2 cut(s) 331, 573
PshBI ATTAAT 1 cut(s) 81
PsiI TTATAA 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 915
Psp6I CCWGG 1 cut(s) 1102
PspGI CCWGG 1 cut(s) 1102
PspPI GGNCC 1 cut(s) 1704
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 1 cut(s) 1417
PsyI GACNNNGTC 1 cut(s) 2084
PvuII CAGCTG 1 cut(s) 1075
RruI TCGCGA 1 cut(s) 1389
SacI GAGCTC 1 cut(s) 915
SapI GCTCTTC 1 cut(s) 682
SatI GCNGC 7 cut(s) 168, 381, 645, 701, 1076, 1267, 1270
Sau3AI GATC 6 cut(s) 234, 495, 627, 946, 1249, 1417
Sau96I GGNCC 1 cut(s) 1704
SchI GAGTC 6 cut(s) 47, 74, 313, 689, 705, 2215
ScrFI CCNGG 1 cut(s) 1104
SduI GDGCHC 2 cut(s) 694, 915
SfaNI GCATC 1 cut(s) 2176
SfcI CTRYAG 2 cut(s) 777, 1559
SfuI TTCGAA 1 cut(s) 2157
SinI GGWCC 1 cut(s) 1704
SmlI CTYRAG 4 cut(s) 107, 301, 1550, 1877
SmoI CTYRAG 4 cut(s) 107, 301, 1550, 1877
SsiI CCGC 1 cut(s) 1702
SspMI CTAG 6 cut(s) 98, 1031, 1343, 1472, 1664, 1682
SstI GAGCTC 1 cut(s) 915
StyD4I CCNGG 1 cut(s) 1102
StyI CCWWGG 1 cut(s) 1663
TaaI ACNGT 5 cut(s) 182, 589, 601, 1018, 1842
TaiI ACGT 1 cut(s) 1579
TaqI TCGA 5 cut(s) 292, 942, 1237, 1252, 2157
TfiI GAWTC 8 cut(s) 674, 902, 935, 1522, 1799, 1873, 1885, 2103
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 7 cut(s) 167, 380, 644, 700, 1075, 1266, 1269
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 8 cut(s) 159, 467, 999, 1179, 1583, 1631, 2050, 2193
TspGWI ACGGA 3 cut(s) 432, 583, 847
Tth111I GACNNNGTC 1 cut(s) 2084
Vha464I CTTAAG 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 1704
VspI ATTAAT 1 cut(s) 81
XapI RAATTY 6 cut(s) 185, 662, 816, 835, 1727, 1768
XceI RCATGY 3 cut(s) 335, 577, 1741
XmaJI CCTAGG 1 cut(s) 1663
XmiI GTMKAC 2 cut(s) 1742, 1915
XspI CTAG 6 cut(s) 98, 1031, 1343, 1472, 1664, 1682
Zsp2I ATGCAT 2 cut(s) 1377, 2155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.