disease resistance
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 17, 1221 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 242, 640 |
| AccI | GTMKAC | 2 cut(s) | 1736, 1909 |
| AccII | CGCG | 1 cut(s) | 1398 |
| AclI | AACGTT | 1 cut(s) | 368 |
| AclWI | GGATC | 9 cut(s) | 518, 631, 677, 690, 733, 746, 950, 1421, 1699 |
| AcoI | YGGCCR | 1 cut(s) | 1472 |
| AcsI | RAATTY | 6 cut(s) | 825, 844, 1090, 1477, 1762, 2090 |
| AcuI | CTGAAG | 3 cut(s) | 332, 1640, 1706 |
| AfaI | GTAC | 1 cut(s) | 1683 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1117 |
| AjnI | CCWGG | 2 cut(s) | 1111, 1675 |
| AleI | CACNNNNGTG | 1 cut(s) | 545 |
| Alw21I | GWGCWC | 2 cut(s) | 703, 924 |
| Alw26I | GTCTC | 4 cut(s) | 618, 712, 1364, 2010 |
| AlwI | GGATC | 9 cut(s) | 518, 631, 677, 690, 733, 746, 950, 1421, 1699 |
| Ama87I | CYCGRG | 1 cut(s) | 686 |
| AoxI | GGCC | 4 cut(s) | 381, 655, 1472, 1751 |
| ApeKI | GCWGC | 3 cut(s) | 173, 1084, 1275 |
| ApoI | RAATTY | 6 cut(s) | 825, 844, 1090, 1477, 1762, 2090 |
| AspA2I | CCTAGG | 1 cut(s) | 1657 |
| AspS9I | GGNCC | 4 cut(s) | 382, 1698, 1751, 1951 |
| AsuHPI | GGTGA | 8 cut(s) | 131, 200, 481, 559, 953, 1665, 1888, 2011 |
| AvaI | CYCGRG | 1 cut(s) | 686 |
| AvaII | GGWCC | 2 cut(s) | 1698, 1951 |
| AvrII | CCTAGG | 1 cut(s) | 1657 |
| BalI | TGGCCA | 1 cut(s) | 1474 |
| BamHI | GGATCC | 2 cut(s) | 682, 738 |
| BanII | GRGCYC | 1 cut(s) | 924 |
| BauI | CACGAG | 1 cut(s) | 1363 |
| BbsI | GAAGAC | 1 cut(s) | 1659 |
| Bbv12I | GWGCWC | 2 cut(s) | 703, 924 |
| BbvI | GCAGC | 3 cut(s) | 160, 1071, 1287 |
| BciT130I | CCWGG | 2 cut(s) | 1113, 1677 |
| BclI | TGATCA | 1 cut(s) | 2014 |
| BcoDI | GTCTC | 4 cut(s) | 618, 712, 1364, 2010 |
| BfaI | CTAG | 6 cut(s) | 1040, 1137, 1352, 1422, 1658, 1709 |
| BfmI | CTRYAG | 3 cut(s) | 786, 1553, 1861 |
| BisI | GCNGC | 3 cut(s) | 174, 1085, 1276 |
| BlnI | CCTAGG | 1 cut(s) | 1657 |
| BlsI | GCNGC | 3 cut(s) | 175, 1086, 1277 |
| BmcAI | AGTACT | 1 cut(s) | 1683 |
| Bme1390I | CCNGG | 2 cut(s) | 1113, 1677 |
| Bme18I | GGWCC | 2 cut(s) | 1698, 1951 |
| BmeT110I | CYCGRG | 1 cut(s) | 686 |
| BmgT120I | GGNCC | 4 cut(s) | 382, 1698, 1751, 1951 |
| BmiI | GGNNCC | 4 cut(s) | 684, 740, 1868, 1952 |
| BmrFI | CCNGG | 2 cut(s) | 1113, 1677 |
| BmrI | ACTGGG | 2 cut(s) | 1094, 2121 |
| BmsI | GCATC | 6 cut(s) | 160, 1060, 1285, 1287, 1594, 1806 |
| BmuI | ACTGGG | 2 cut(s) | 1094, 2121 |
| BpiI | GAAGAC | 1 cut(s) | 1659 |
| BpmI | CTGGAG | 2 cut(s) | 1134, 1882 |
| BpuEI | CTTGAG | 3 cut(s) | 331, 1565, 1892 |
| Bsa29I | ATCGAT | 5 cut(s) | 301, 1412, 1435, 2136, 2140 |
| BsaBI | GATNNNNATC | 3 cut(s) | 509, 681, 1431 |
| BsaJI | CCNNGG | 4 cut(s) | 1111, 1657, 1811, 1954 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 298, 328 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1117 |
| Bse1I | ACTGG | 5 cut(s) | 640, 1100, 1509, 1526, 2116 |
| Bse8I | GATNNNNATC | 3 cut(s) | 509, 681, 1431 |
| BseBI | CCWGG | 2 cut(s) | 1113, 1677 |
| BseCI | ATCGAT | 5 cut(s) | 301, 1412, 1435, 2136, 2140 |
| BseDI | CCNNGG | 4 cut(s) | 1111, 1657, 1811, 1954 |
| BseGI | GGATG | 6 cut(s) | 274, 385, 1107, 1570, 1986, 2043 |
| BseJI | GATNNNNATC | 3 cut(s) | 509, 681, 1431 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1117 |
| BseMII | CTCAG | 2 cut(s) | 1789, 1986 |
| BseNI | ACTGG | 5 cut(s) | 640, 1100, 1509, 1526, 2116 |
| BseRI | GAGGAG | 1 cut(s) | 540 |
| BseXI | GCAGC | 3 cut(s) | 160, 1071, 1287 |
| BsgI | GTGCAG | 2 cut(s) | 176, 375 |
| Bsh1236I | CGCG | 1 cut(s) | 1398 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2140 |
| BshFI | GGCC | 4 cut(s) | 383, 657, 1474, 1753 |
| BshVI | ATCGAT | 5 cut(s) | 301, 1412, 1435, 2136, 2140 |
| BsiEI | CGRYCG | 1 cut(s) | 2140 |
| BsiHKAI | GWGCWC | 2 cut(s) | 703, 924 |
| BsiHKCI | CYCGRG | 1 cut(s) | 686 |
| BslFI | GGGAC | 2 cut(s) | 907, 1964 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1117 |
| BsmAI | GTCTC | 4 cut(s) | 618, 712, 1364, 2010 |
| BsmFI | GGGAC | 2 cut(s) | 907, 1964 |
| BsmI | GAATGC | 1 cut(s) | 1312 |
| BsnI | GGCC | 4 cut(s) | 383, 657, 1474, 1753 |
| BsoBI | CYCGRG | 1 cut(s) | 686 |
| Bsp1286I | GDGCHC | 2 cut(s) | 703, 924 |
| Bsp68I | TCGCGA | 1 cut(s) | 1398 |
| BspANI | GGCC | 4 cut(s) | 383, 657, 1474, 1753 |
| BspCNI | CTCAG | 2 cut(s) | 1788, 1987 |
| BspDI | ATCGAT | 5 cut(s) | 301, 1412, 1435, 2136, 2140 |
| BspFNI | CGCG | 1 cut(s) | 1398 |
| BspLI | GGNNCC | 4 cut(s) | 684, 740, 1868, 1952 |
| BspMAI | CTGCAG | 1 cut(s) | 1865 |
| BspPI | GGATC | 9 cut(s) | 518, 631, 677, 690, 733, 746, 950, 1421, 1699 |
| BsrI | ACTGG | 5 cut(s) | 640, 1100, 1509, 1526, 2116 |
| BssECI | CCNNGG | 4 cut(s) | 1111, 1657, 1811, 1954 |
| BssSI | CACGAG | 1 cut(s) | 1363 |
| BssT1I | CCWWGG | 2 cut(s) | 1657, 1954 |
| Bst2BI | CACGAG | 1 cut(s) | 1363 |
| Bst2UI | CCWGG | 2 cut(s) | 1113, 1677 |
| Bst4CI | ACNGT | 3 cut(s) | 188, 610, 1027 |
| Bst6I | CTCTTC | 3 cut(s) | 479, 1911, 1960 |
| BstC8I | GCNNGC | 3 cut(s) | 655, 1059, 1987 |
| BstDEI | CTNAG | 4 cut(s) | 113, 1775, 1995, 2065 |
| BstF5I | GGATG | 6 cut(s) | 274, 385, 1107, 1570, 1986, 2043 |
| BstFNI | CGCG | 1 cut(s) | 1398 |
| BstMAI | GTCTC | 4 cut(s) | 618, 712, 1364, 2010 |
| BstMCI | CGRYCG | 1 cut(s) | 2140 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 389 |
| BstNI | CCWGG | 2 cut(s) | 1113, 1677 |
| BstNSI | RCATGY | 3 cut(s) | 344, 1061, 1735 |
| BstSCI | CCNGG | 2 cut(s) | 1111, 1675 |
| BstSFI | CTRYAG | 3 cut(s) | 786, 1553, 1861 |
| BstUI | CGCG | 1 cut(s) | 1398 |
| BstV1I | GCAGC | 3 cut(s) | 160, 1071, 1287 |
| BstV2I | GAAGAC | 1 cut(s) | 1659 |
| BstX2I | RGATCY | 4 cut(s) | 510, 682, 738, 1426 |
| BstYI | RGATCY | 4 cut(s) | 510, 682, 738, 1426 |
| Bsu15I | ATCGAT | 5 cut(s) | 301, 1412, 1435, 2136, 2140 |
| BsuRI | GGCC | 4 cut(s) | 383, 657, 1474, 1753 |
| BsuTUI | ATCGAT | 5 cut(s) | 301, 1412, 1435, 2136, 2140 |
| BtgZI | GCGATG | 3 cut(s) | 304, 1413, 1622 |
| BtsCI | GGATG | 6 cut(s) | 274, 385, 1107, 1570, 1986, 2043 |
| BtsIMutI | CAGTG | 2 cut(s) | 1107, 1502 |
| BtuMI | TCGCGA | 1 cut(s) | 1398 |
| Cac8I | GCNNGC | 3 cut(s) | 655, 1059, 1987 |
| Cfr13I | GGNCC | 4 cut(s) | 382, 1698, 1751, 1951 |
| ClaI | ATCGAT | 5 cut(s) | 301, 1412, 1435, 2136, 2140 |
| Csp6I | GTAC | 1 cut(s) | 1682 |
| CviQI | GTAC | 1 cut(s) | 1682 |
| DdeI | CTNAG | 4 cut(s) | 113, 1775, 1995, 2065 |
| DraI | TTTAAA | 1 cut(s) | 433 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 242, 640 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 242, 640 |
| EaeI | YGGCCR | 1 cut(s) | 1472 |
| Eam1104I | CTCTTC | 3 cut(s) | 479, 1911, 1960 |
| EarI | CTCTTC | 3 cut(s) | 479, 1911, 1960 |
| Ecl136II | GAGCTC | 1 cut(s) | 922 |
| Eco130I | CCWWGG | 2 cut(s) | 1657, 1954 |
| Eco24I | GRGCYC | 1 cut(s) | 924 |
| Eco47I | GGWCC | 2 cut(s) | 1698, 1951 |
| Eco53kI | GAGCTC | 1 cut(s) | 922 |
| Eco57I | CTGAAG | 3 cut(s) | 332, 1640, 1706 |
| Eco88I | CYCGRG | 1 cut(s) | 686 |
| EcoICRI | GAGCTC | 1 cut(s) | 922 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1951 |
| EcoRII | CCWGG | 2 cut(s) | 1111, 1675 |
| EcoT14I | CCWWGG | 2 cut(s) | 1657, 1954 |
| EcoT22I | ATGCAT | 2 cut(s) | 1063, 1386 |
| EcoT38I | GRGCYC | 1 cut(s) | 924 |
| ErhI | CCWWGG | 2 cut(s) | 1657, 1954 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1059, 1091 |
| FaqI | GGGAC | 2 cut(s) | 907, 1964 |
| FauNDI | CATATG | 1 cut(s) | 1489 |
| FbaI | TGATCA | 1 cut(s) | 2014 |
| FblI | GTMKAC | 2 cut(s) | 1736, 1909 |
| Fnu4HI | GCNGC | 3 cut(s) | 174, 1085, 1276 |
| FokI | GGATG | 6 cut(s) | 281, 372, 1094, 1577, 1993, 2030 |
| FriOI | GRGCYC | 1 cut(s) | 924 |
| Fsp4HI | GCNGC | 3 cut(s) | 174, 1085, 1276 |
| FspBI | CTAG | 6 cut(s) | 1040, 1137, 1352, 1422, 1658, 1709 |
| GluI | GCNGC | 3 cut(s) | 174, 1085, 1276 |
| GsuI | CTGGAG | 2 cut(s) | 1134, 1882 |
| HaeIII | GGCC | 4 cut(s) | 383, 657, 1474, 1753 |
| HincII | GTYRAC | 4 cut(s) | 414, 834, 875, 1324 |
| HindII | GTYRAC | 4 cut(s) | 414, 834, 875, 1324 |
| HindIII | AAGCTT | 1 cut(s) | 131 |
| HinfI | GANTC | 7 cut(s) | 313, 689, 720, 911, 944, 1522, 1879 |
| HphI | GGTGA | 8 cut(s) | 131, 200, 481, 559, 953, 1665, 1888, 2011 |
| Hpy188I | TCNGA | 6 cut(s) | 213, 318, 646, 1720, 1996, 2014 |
| Hpy99I | CGWCG | 1 cut(s) | 239 |
| HpyAV | CCTTC | 7 cut(s) | 405, 419, 958, 985, 1332, 1615, 2017 |
| HpyCH4III | ACNGT | 3 cut(s) | 188, 610, 1027 |
| HpyCH4IV | ACGT | 2 cut(s) | 368, 1570 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 389 |
| HpyF3I | CTNAG | 4 cut(s) | 113, 1775, 1995, 2065 |
| HpySE526I | ACGT | 2 cut(s) | 368, 1570 |
| Ksp22I | TGATCA | 1 cut(s) | 2014 |
| LmnI | GCTCC | 3 cut(s) | 306, 357, 919 |
| Lsp1109I | GCAGC | 3 cut(s) | 160, 1071, 1287 |
| LweI | GCATC | 6 cut(s) | 160, 1060, 1285, 1287, 1594, 1806 |
| MaeI | CTAG | 6 cut(s) | 1040, 1137, 1352, 1422, 1658, 1709 |
| MaeII | ACGT | 2 cut(s) | 368, 1570 |
| MaeIII | GTNAC | 4 cut(s) | 52, 1021, 1282, 1347 |
| MfeI | CAATTG | 3 cut(s) | 24, 1230, 1835 |
| MflI | RGATCY | 4 cut(s) | 510, 682, 738, 1426 |
| MhlI | GDGCHC | 2 cut(s) | 703, 924 |
| MlsI | TGGCCA | 1 cut(s) | 1474 |
| MluNI | TGGCCA | 1 cut(s) | 1474 |
| MlyI | GAGTC | 3 cut(s) | 322, 698, 714 |
| MmeI | TCCRAC | 4 cut(s) | 123, 296, 624, 1009 |
| Mox20I | TGGCCA | 1 cut(s) | 1474 |
| Mph1103I | ATGCAT | 2 cut(s) | 1063, 1386 |
| MscI | TGGCCA | 1 cut(s) | 1474 |
| MseI | TTAA | 9 cut(s) | 87, 276, 432, 441, 630, 780, 899, 1077, 1830 |
| MslI | CAYNNNNRTG | 1 cut(s) | 545 |
| Msp20I | TGGCCA | 1 cut(s) | 1474 |
| MspA1I | CMGCKG | 1 cut(s) | 1084 |
| MspR9I | CCNGG | 2 cut(s) | 1113, 1677 |
| MunI | CAATTG | 3 cut(s) | 24, 1230, 1835 |
| Mva1269I | GAATGC | 1 cut(s) | 1312 |
| MvaI | CCWGG | 2 cut(s) | 1113, 1677 |
| MvnI | CGCG | 1 cut(s) | 1398 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 389 |
| NdeI | CATATG | 1 cut(s) | 1489 |
| NlaIV | GGNNCC | 4 cut(s) | 684, 740, 1868, 1952 |
| NmeAIII | GCCGAG | 1 cut(s) | 1836 |
| NmuCI | GTSAC | 1 cut(s) | 52 |
| NruI | TCGCGA | 1 cut(s) | 1398 |
| NsiI | ATGCAT | 2 cut(s) | 1063, 1386 |
| NspI | RCATGY | 3 cut(s) | 344, 1061, 1735 |
| OliI | CACNNNNGTG | 1 cut(s) | 545 |
| PaeI | GCATGC | 1 cut(s) | 1061 |
| PctI | GAATGC | 1 cut(s) | 1312 |
| PfeI | GAWTC | 4 cut(s) | 911, 944, 1522, 1879 |
| PflFI | GACNNNGTC | 2 cut(s) | 242, 2066 |
| PkrI | GCNGC | 3 cut(s) | 175, 1086, 1277 |
| Ple19I | CGATCG | 1 cut(s) | 2140 |
| PleI | GAGTC | 3 cut(s) | 321, 697, 714 |
| PpsI | GAGTC | 3 cut(s) | 321, 697, 714 |
| PpuMI | RGGWCCY | 1 cut(s) | 1951 |
| PsiI | TTATAA | 2 cut(s) | 17, 1221 |
| Psp124BI | GAGCTC | 1 cut(s) | 924 |
| Psp1406I | AACGTT | 1 cut(s) | 368 |
| Psp5II | RGGWCCY | 1 cut(s) | 1951 |
| Psp6I | CCWGG | 2 cut(s) | 1111, 1675 |
| PspGI | CCWGG | 2 cut(s) | 1111, 1675 |
| PspN4I | GGNNCC | 4 cut(s) | 684, 740, 1868, 1952 |
| PspPI | GGNCC | 4 cut(s) | 382, 1698, 1751, 1951 |
| PspPPI | RGGWCCY | 1 cut(s) | 1951 |
| PstI | CTGCAG | 1 cut(s) | 1865 |
| PsuI | RGATCY | 4 cut(s) | 510, 682, 738, 1426 |
| PsyI | GACNNNGTC | 2 cut(s) | 242, 2066 |
| PvuI | CGATCG | 1 cut(s) | 2140 |
| PvuII | CAGCTG | 1 cut(s) | 1084 |
| RruI | TCGCGA | 1 cut(s) | 1398 |
| RsaI | GTAC | 1 cut(s) | 1683 |
| RsaNI | GTAC | 1 cut(s) | 1682 |
| RseI | CAYNNNNRTG | 1 cut(s) | 545 |
| SacI | GAGCTC | 1 cut(s) | 924 |
| SaqAI | TTAA | 9 cut(s) | 87, 276, 432, 441, 630, 780, 899, 1077, 1830 |
| SatI | GCNGC | 3 cut(s) | 174, 1085, 1276 |
| Sau96I | GGNCC | 4 cut(s) | 382, 1698, 1751, 1951 |
| ScaI | AGTACT | 1 cut(s) | 1683 |
| SchI | GAGTC | 3 cut(s) | 322, 698, 714 |
| ScrFI | CCNGG | 2 cut(s) | 1113, 1677 |
| SduI | GDGCHC | 2 cut(s) | 703, 924 |
| SfaNI | GCATC | 6 cut(s) | 160, 1060, 1285, 1287, 1594, 1806 |
| SfcI | CTRYAG | 3 cut(s) | 786, 1553, 1861 |
| SinI | GGWCC | 2 cut(s) | 1698, 1951 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 545 |
| SmlI | CTYRAG | 3 cut(s) | 310, 1544, 1871 |
| SmoI | CTYRAG | 3 cut(s) | 310, 1544, 1871 |
| SphI | GCATGC | 1 cut(s) | 1061 |
| SspI | AATATT | 1 cut(s) | 1225 |
| SspMI | CTAG | 6 cut(s) | 1040, 1137, 1352, 1422, 1658, 1709 |
| SstI | GAGCTC | 1 cut(s) | 924 |
| StyD4I | CCNGG | 2 cut(s) | 1111, 1675 |
| StyI | CCWWGG | 2 cut(s) | 1657, 1954 |
| TaaI | ACNGT | 3 cut(s) | 188, 610, 1027 |
| TaiI | ACGT | 2 cut(s) | 371, 1573 |
| TaqI | TCGA | 8 cut(s) | 301, 951, 1246, 1261, 1412, 1435, 2136, 2140 |
| TatI | WGTACW | 1 cut(s) | 1681 |
| TfiI | GAWTC | 4 cut(s) | 911, 944, 1522, 1879 |
| Tru1I | TTAA | 9 cut(s) | 87, 276, 432, 441, 630, 780, 899, 1077, 1830 |
| Tru9I | TTAA | 9 cut(s) | 87, 276, 432, 441, 630, 780, 899, 1077, 1830 |
| TscAI | CASTG | 2 cut(s) | 1107, 1509 |
| TseFI | GTSAC | 1 cut(s) | 52 |
| TseI | GCWGC | 3 cut(s) | 173, 1084, 1275 |
| Tsp45I | GTSAC | 1 cut(s) | 52 |
| TspDTI | ATGAA | 3 cut(s) | 285, 1188, 1625 |
| TspGWI | ACGGA | 4 cut(s) | 23, 740, 856, 2084 |
| TspRI | CASTG | 2 cut(s) | 1107, 1509 |
| Tth111I | GACNNNGTC | 2 cut(s) | 242, 2066 |
| VpaK11BI | GGWCC | 2 cut(s) | 1698, 1951 |
| XapI | RAATTY | 6 cut(s) | 825, 844, 1090, 1477, 1762, 2090 |
| XbaI | TCTAGA | 1 cut(s) | 1421 |
| XceI | RCATGY | 3 cut(s) | 344, 1061, 1735 |
| XmaJI | CCTAGG | 1 cut(s) | 1657 |
| XmiI | GTMKAC | 2 cut(s) | 1736, 1909 |
| XspI | CTAG | 6 cut(s) | 1040, 1137, 1352, 1422, 1658, 1709 |
| ZrmI | AGTACT | 1 cut(s) | 1683 |
| Zsp2I | ATGCAT | 2 cut(s) | 1063, 1386 |