MD03G1179300.v1.1

Belongs to the Nudix hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004263
Physical Location & Seq
Reverse (-)
24638815 .. 24641458
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1179300.v1.1.491

Sequence Viewer

Length: 585 bp
ATGTTTAAAGAGGGTTTTCGGTACCATCATGCTGAGCCGCACTACCTCATGCTTGTTTATTGGATTCCTGAAATTAATGATACTCTACCTGCAAATGCATCACACCGACTAGGTGTTGGTGCTATTGTTTTGAATGATAAAAAAGAGATGCTTGTAGTCCAGGAAAAGAGTGGCAGGTTCCAAGGAGCGGGGGTGTGGAAAATCCCAACTGGAGTCGTTGACGAGGGTGAAAATATATTTGTAGCTGTCGTAAGAGAAGTTAAAGAAGAAACAGGACACACAAGACCCCTTGAGAAGTCGGATCTAATCTTCTTGTGCATGCTGCGTCCTCTAACCTTTGACATCCAAAAGCAAGAACTGGAGATCAAGGCGGCCAAGTGGATTCCACTTGAGGAGTTTGCAGCTCAACCAGTCACCCAGAAACATGAGCTCTTCAAGTACATTCGTGAACTTTGCTCGGCAAAGTTAGACAGAGAATATGCTGGATTTTCTCCACTGCCTATAACATCAGTTTTTGACCATAAGTTGAGTTATTTGTATGTAAATAGGCAGGATAAGCAGTGCCTATGTTCTTCTGATCAGTGA

Protein Analysis

195

Amino Acids

22.38

Weight (kDa)

6.17

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 35 - 142 7.9e-19 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 97, 165
Acc65I GGTACC 1 cut(s) 21
AccB1I GGYRCC 1 cut(s) 21
AccBSI CCGCTC 1 cut(s) 188
AciI CCGC 3 cut(s) 38, 188, 371
AclWI GGATC 1 cut(s) 309
AcoI YGGCCR 1 cut(s) 372
AfaI GTAC 2 cut(s) 23, 440
AfiI CCNNNNNNNGG 1 cut(s) 187
AgsI TTSAA 2 cut(s) 133, 436
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 3 cut(s) 245, 404, 430
AluI AGCT 3 cut(s) 245, 404, 430
Alw21I GWGCWC 1 cut(s) 432
AlwI GGATC 1 cut(s) 309
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 322, 401
AseI ATTAAT 1 cut(s) 75
Asp718I GGTACC 1 cut(s) 21
AsuHPI GGTGA 2 cut(s) 239, 406
BanI GGYRCC 1 cut(s) 21
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 309, 413
BccI CCATC 1 cut(s) 33
BciT130I CCWGG 1 cut(s) 161
BclI TGATCA 1 cut(s) 577
BfaI CTAG 1 cut(s) 110
BfuAI ACCTGC 2 cut(s) 97, 165
BisI GCNGC 4 cut(s) 38, 323, 372, 402
BlpI GCTNAGC 1 cut(s) 33
BlsI GCNGC 4 cut(s) 39, 324, 373, 403
Bme1390I CCNGG 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 23, 179
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 2 cut(s) 107, 138
BpmI CTGGAG 2 cut(s) 231, 380
Bpu1102I GCTNAGC 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 311, 410
BsaJI CCNNGG 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse1I ACTGG 3 cut(s) 214, 363, 410
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 3 cut(s) 214, 363, 410
BseRI GAGGAG 1 cut(s) 407
BseXI GCAGC 2 cut(s) 309, 413
BshFI GGCC 1 cut(s) 374
BshNI GGYRCC 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 432
BslI CCNNNNNNNGG 1 cut(s) 187
BsnI GGCC 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 3 cut(s) 301, 363, 577
Bsp1720I GCTNAGC 1 cut(s) 33
BspACI CCGC 3 cut(s) 38, 188, 371
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 2 cut(s) 23, 179
BspMI ACCTGC 2 cut(s) 97, 165
BspPI GGATC 1 cut(s) 309
BspQI GCTCTTC 1 cut(s) 437
BspT107I GGYRCC 1 cut(s) 21
BsrBI CCGCTC 1 cut(s) 188
BsrI ACTGG 3 cut(s) 214, 363, 410
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 3 cut(s) 301, 363, 577
BssT1I CCWWGG 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 161
Bst6I CTCTTC 1 cut(s) 437
BstC8I GCNNGC 1 cut(s) 320
BstDEI CTNAG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 342
BstKTI GATC 3 cut(s) 304, 366, 580
BstMBI GATC 3 cut(s) 301, 363, 577
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNI CCWGG 1 cut(s) 161
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 309, 413
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 1 cut(s) 374
BtsCI GGATG 1 cut(s) 342
BtsI GCAGTG 2 cut(s) 494, 566
BtsIMutI CAGTG 2 cut(s) 494, 566
BveI ACCTGC 2 cut(s) 97, 165
Cac8I GCNNGC 1 cut(s) 320
CseI GACGC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 22, 439
CviAII CATG 4 cut(s) 29, 49, 319, 425
CviJI RGCY 5 cut(s) 37, 245, 374, 404, 430
CviKI_1 RGCY 5 cut(s) 37, 245, 374, 404, 430
CviQI GTAC 2 cut(s) 22, 439
DdeI CTNAG 1 cut(s) 33
DpnI GATC 3 cut(s) 303, 365, 579
DpnII GATC 3 cut(s) 301, 363, 577
DraI TTTAAA 1 cut(s) 7
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 432
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 181
EcoT22I ATGCAT 1 cut(s) 100
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 181
FaeI CATG 4 cut(s) 32, 52, 322, 428
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FatI CATG 4 cut(s) 28, 48, 318, 424
FauI CCCGC 1 cut(s) 181
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 4 cut(s) 38, 323, 372, 402
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 4 cut(s) 38, 323, 372, 402
FspBI CTAG 1 cut(s) 110
GluI GCNGC 4 cut(s) 38, 323, 372, 402
GsuI CTGGAG 2 cut(s) 231, 380
HaeIII GGCC 1 cut(s) 374
HgaI GACGC 1 cut(s) 314
Hin1II CATG 4 cut(s) 32, 52, 322, 428
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 3 cut(s) 64, 213, 382
HphI GGTGA 2 cut(s) 239, 406
Hpy166II GTNNAC 2 cut(s) 220, 449
Hpy188I TCNGA 2 cut(s) 301, 577
Hpy188III TCNNGA 2 cut(s) 68, 446
Hpy8I GTNNAC 2 cut(s) 220, 449
HpyCH4V TGCA 4 cut(s) 92, 98, 318, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpyF3I CTNAG 1 cut(s) 33
Hsp92II CATG 4 cut(s) 32, 52, 322, 428
KpnI GGTACC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 3 cut(s) 301, 363, 577
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 1 cut(s) 185
Lsp1109I GCAGC 2 cut(s) 309, 413
LweI GCATC 2 cut(s) 107, 138
MaeI CTAG 1 cut(s) 110
MaeIII GTNAC 1 cut(s) 412
MalI GATC 3 cut(s) 303, 365, 579
MbiI CCGCTC 1 cut(s) 188
MboI GATC 3 cut(s) 301, 363, 577
MboII GAAGA 4 cut(s) 278, 301, 424, 564
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 1 cut(s) 72
MlyI GAGTC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 5 cut(s) 4, 56, 217, 339, 385
Mph1103I ATGCAT 1 cut(s) 100
MseI TTAA 3 cut(s) 6, 75, 261
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 1 cut(s) 556
NdeII GATC 3 cut(s) 301, 363, 577
NlaIII CATG 4 cut(s) 32, 52, 322, 428
NlaIV GGNNCC 2 cut(s) 23, 179
NmeAIII GCCGAG 1 cut(s) 437
NmuCI GTSAC 1 cut(s) 412
NsiI ATGCAT 1 cut(s) 100
NspI RCATGY 1 cut(s) 322
PaeI GCATGC 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 437
PfeI GAWTC 2 cut(s) 64, 382
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 4 cut(s) 39, 324, 373, 403
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PshBI ATTAAT 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 23, 179
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 2 cut(s) 23, 440
RsaNI GTAC 2 cut(s) 22, 439
SacI GAGCTC 1 cut(s) 432
SapI GCTCTTC 1 cut(s) 437
SaqAI TTAA 3 cut(s) 6, 75, 261
SatI GCNGC 4 cut(s) 38, 323, 372, 402
Sau3AI GATC 3 cut(s) 301, 363, 577
SchI GAGTC 1 cut(s) 222
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 1 cut(s) 432
SetI ASST 8 cut(s) 48, 91, 115, 179, 247, 338, 406, 432
SfaNI GCATC 2 cut(s) 107, 138
SmlI CTYRAG 2 cut(s) 290, 389
SmoI CTYRAG 2 cut(s) 290, 389
SphI GCATGC 1 cut(s) 322
Sse9I AATT 1 cut(s) 72
SsiI CCGC 3 cut(s) 38, 188, 371
SspMI CTAG 1 cut(s) 110
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 181
TasI AATT 1 cut(s) 72
TatI WGTACW 1 cut(s) 438
TauI GCSGC 2 cut(s) 40, 374
TfiI GAWTC 2 cut(s) 64, 382
Tru1I TTAA 3 cut(s) 6, 75, 261
Tru9I TTAA 3 cut(s) 6, 75, 261
TscAI CASTG 2 cut(s) 501, 566
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 2 cut(s) 322, 401
Tsp45I GTSAC 1 cut(s) 412
TspRI CASTG 2 cut(s) 501, 566
VspI ATTAAT 1 cut(s) 75
XceI RCATGY 1 cut(s) 322
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XspI CTAG 1 cut(s) 110
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.