MD03G1194100.v1.1
NAC Family

Nascent polypeptide-associated complex subunit alpha-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011752
Physical Location & Seq
Reverse (-)
26627219 .. 26630967
3749 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1194100.v1.1.491

Sequence Viewer

Length: 624 bp
ATGTCTTCGCTGCTTGGCATCATGACTGCCCAAACTCAAGAAGAGCTCCTTGCTGCCCAGCTTGAACAGCAAAAAGTTCATGATGATGAACCGATAGTCGAGGACGAAGATGATGACGATGATGATGATGATGAAGATGACGACCATGATGACGATGTTGAAGGACAAGGAGATGGAAGTGGTAGGTCTAAGCAGAGCAGAAGTGAGAAGAAGAGTCGCAAGGCGATGTTGAAACTTGGAATGAAAGCAATTCCAGGTGTCAGCCGTGTGACTGTCAAGAAGAGCAAGAATATTCTGTTTGTCATCTCAAAGCCAGATGTCTTTAAGAGCCCCGCTTCCGATACATATGTCATTTTTGGGGAAGCAAAGATTGAGGACTTGAGCTCACAACTTCAAACTCAGGCCGCGGAGCAGTTCAAGGCTCCTAACCTAAATACTGTGACAGCAAAGCCCGAGCCATCAACTTTGGCTCAAGACGATGAAGATGTGGACGAAACTGGTGTAGAACCCAAGGACATCGAGTTGGTGATGACTCAAGCTGGGGTTTCAAGATCAAGAGCAGTTAAGGCACTCAAAGCAGCAAATGGTGACATTGTTTCTGCCATCATGGAATTAACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAC

Protein Analysis

208

Amino Acids

22.52

Weight (kDa)

4.36

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAC PF01849 69 - 124 5.5e-23 NAC domain
UBA_HYPK PF19026 167 - 206 7.3e-13 HYPK UBA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011752)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12390 AT5G13850
fragaria_vesca FvH4_7g04351
malus_domestica MD03G1194100.v1.1 MD11G1210600.v1.1
prunus_persica Prupe.2G049200_v2.0.a1 Prupe.7G020000_v2.0.a1
pyrus_communis pycom03g14650 pycom11g18620
rosa_chinensis RchiOBHm_Chr1g0329361
rosa_laevigata RLG00000029899
rosa_multiflora Rmu_sc0001359.1_g000007
rosa_roxburghii Rroxscaffold_4G00321180
rosa_rugosa Rorug01G0078800
rosa_samantha Rh1AG097100 Rh1BG077700 Rh1CG094000 Rh1DG100700
rosa_wichuraiana Rw1G007600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 407
AciI CCGC 3 cut(s) 333, 405, 407
AgsI TTSAA 6 cut(s) 65, 161, 232, 395, 418, 549
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 4 cut(s) 46, 61, 384, 539
AluI AGCT 4 cut(s) 46, 61, 384, 539
Alw21I GWGCWC 2 cut(s) 48, 386
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 3 cut(s) 10, 53, 578
AsuHPI GGTGA 2 cut(s) 538, 599
AvaI CYCGRG 1 cut(s) 452
BaeI ACNNNNGTAYC 2 cut(s) 333, 366
BanII GRGCYC 3 cut(s) 48, 332, 386
Bbv12I GWGCWC 2 cut(s) 48, 386
BbvI GCAGC 2 cut(s) 40, 590
BccI CCATC 3 cut(s) 167, 466, 611
BceAI ACGGC 1 cut(s) 249
BciT130I CCWGG 1 cut(s) 255
BisI GCNGC 4 cut(s) 11, 54, 405, 579
BlsI GCNGC 4 cut(s) 12, 55, 406, 580
Bme1390I CCNGG 1 cut(s) 255
BmeT110I CYCGRG 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 1 cut(s) 27
BpuEI CTTGAG 4 cut(s) 21, 400, 456, 519
BsaJI CCNNGG 2 cut(s) 405, 510
Bse1I ACTGG 1 cut(s) 502
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 2 cut(s) 405, 510
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 1 cut(s) 502
BseXI GCAGC 2 cut(s) 40, 590
BseYI CCCAGC 2 cut(s) 57, 539
Bsh1236I CGCG 1 cut(s) 407
BshFI GGCC 1 cut(s) 404
BsiHKAI GWGCWC 2 cut(s) 48, 386
BsiHKCI CYCGRG 1 cut(s) 452
BsnI GGCC 1 cut(s) 404
BsoBI CYCGRG 1 cut(s) 452
Bsp1286I GDGCHC 3 cut(s) 48, 332, 386
Bsp143I GATC 1 cut(s) 551
BspACI CCGC 3 cut(s) 333, 405, 407
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 407
BspHI TCATGA 2 cut(s) 21, 79
BspLI GGNNCC 1 cut(s) 423
BspQI GCTCTTC 2 cut(s) 36, 275
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 2 cut(s) 405, 510
BssMI GATC 1 cut(s) 551
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 2 cut(s) 274, 439
Bst6I CTCTTC 3 cut(s) 36, 206, 275
BstDEI CTNAG 2 cut(s) 189, 399
BstDSI CCRYGG 1 cut(s) 405
BstFNI CGCG 1 cut(s) 407
BstKTI GATC 1 cut(s) 554
BstMBI GATC 1 cut(s) 551
BstMWI GCNNNNNNNGC 3 cut(s) 67, 566, 575
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 2 cut(s) 40, 590
BsuRI GGCC 1 cut(s) 404
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 1 cut(s) 239
CciI TCATGA 2 cut(s) 21, 79
Cfr42I CCGCGG 1 cut(s) 408
CviAII CATG 4 cut(s) 22, 80, 146, 607
DdeI CTNAG 2 cut(s) 189, 399
DpnI GATC 1 cut(s) 553
DpnII GATC 1 cut(s) 551
Eam1104I CTCTTC 3 cut(s) 36, 206, 275
EarI CTCTTC 3 cut(s) 36, 206, 275
Ecl136II GAGCTC 2 cut(s) 46, 384
Eco130I CCWWGG 1 cut(s) 510
Eco24I GRGCYC 3 cut(s) 48, 332, 386
Eco53kI GAGCTC 2 cut(s) 46, 384
Eco88I CYCGRG 1 cut(s) 452
EcoICRI GAGCTC 2 cut(s) 46, 384
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 510
EcoT38I GRGCYC 3 cut(s) 48, 332, 386
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 4 cut(s) 25, 83, 149, 610
FaiI YATR 6 cut(s) 23, 81, 147, 346, 348, 608
FalI AAGNNNNNCTT 2 cut(s) 33, 65
FatI CATG 4 cut(s) 21, 79, 145, 606
FauI CCCGC 1 cut(s) 340
FauNDI CATATG 1 cut(s) 346
Fnu4HI GCNGC 4 cut(s) 11, 54, 405, 579
FriOI GRGCYC 3 cut(s) 48, 332, 386
Fsp4HI GCNGC 4 cut(s) 11, 54, 405, 579
GluI GCNGC 4 cut(s) 11, 54, 405, 579
GsaI CCCAGC 2 cut(s) 61, 543
HaeIII GGCC 1 cut(s) 404
Hin1II CATG 4 cut(s) 25, 83, 149, 610
HinfI GANTC 2 cut(s) 214, 532
HphI GGTGA 2 cut(s) 538, 599
Hpy166II GTNNAC 1 cut(s) 490
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 7 cut(s) 22, 38, 80, 277, 473, 549, 555
Hpy8I GTNNAC 1 cut(s) 490
HpyAV CCTTC 1 cut(s) 155
HpyCH4III ACNGT 2 cut(s) 274, 439
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 566, 575
HpyF3I CTNAG 2 cut(s) 189, 399
Hsp92II CATG 4 cut(s) 25, 83, 149, 610
KspI CCGCGG 1 cut(s) 408
Kzo9I GATC 1 cut(s) 551
LguI GCTCTTC 2 cut(s) 36, 275
LmnI GCTCC 3 cut(s) 51, 409, 427
LpnPI CCDG 7 cut(s) 71, 240, 267, 327, 386, 483, 525
Lsp1109I GCAGC 2 cut(s) 40, 590
LweI GCATC 1 cut(s) 27
MaeIII GTNAC 3 cut(s) 268, 439, 587
MalI GATC 1 cut(s) 553
MboI GATC 1 cut(s) 551
MboII GAAGA 7 cut(s) 53, 119, 146, 220, 223, 292, 494
MhlI GDGCHC 3 cut(s) 48, 332, 386
MluCI AATT 2 cut(s) 249, 611
MlyI GAGTC 2 cut(s) 223, 526
MnlI CCTC 2 cut(s) 94, 367
MseI TTAA 3 cut(s) 324, 564, 614
MslI CAYNNNNRTG 1 cut(s) 84
MspA1I CMGCKG 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 255
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 3 cut(s) 67, 566, 575
NdeI CATATG 1 cut(s) 346
NdeII GATC 1 cut(s) 551
NlaIII CATG 4 cut(s) 25, 83, 149, 610
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 3 cut(s) 268, 439, 587
PagI TCATGA 2 cut(s) 21, 79
PciSI GCTCTTC 2 cut(s) 36, 275
PkrI GCNGC 4 cut(s) 12, 55, 406, 580
PleI GAGTC 2 cut(s) 222, 526
PpsI GAGTC 2 cut(s) 222, 526
Psp124BI GAGCTC 2 cut(s) 48, 386
Psp6I CCWGG 1 cut(s) 253
PspFI CCCAGC 2 cut(s) 57, 539
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 423
RseI CAYNNNNRTG 1 cut(s) 84
SacI GAGCTC 2 cut(s) 48, 386
SacII CCGCGG 1 cut(s) 408
SapI GCTCTTC 2 cut(s) 36, 275
SaqAI TTAA 3 cut(s) 324, 564, 614
SatI GCNGC 4 cut(s) 11, 54, 405, 579
Sau3AI GATC 1 cut(s) 551
SchI GAGTC 2 cut(s) 223, 526
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 3 cut(s) 48, 332, 386
SetI ASST 7 cut(s) 48, 63, 188, 259, 386, 432, 541
SfaNI GCATC 1 cut(s) 27
Sfr303I CCGCGG 1 cut(s) 408
SgrBI CCGCGG 1 cut(s) 408
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 4 cut(s) 36, 379, 471, 534
SmoI CTYRAG 4 cut(s) 36, 379, 471, 534
Sse9I AATT 2 cut(s) 249, 611
SsiI CCGC 3 cut(s) 333, 405, 407
SspI AATATT 1 cut(s) 292
SstI GAGCTC 2 cut(s) 48, 386
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 274, 439
TaqI TCGA 2 cut(s) 99, 519
TasI AATT 2 cut(s) 249, 611
TauI GCSGC 1 cut(s) 407
Tru1I TTAA 3 cut(s) 324, 564, 614
Tru9I TTAA 3 cut(s) 324, 564, 614
TseFI GTSAC 3 cut(s) 268, 439, 587
TseI GCWGC 3 cut(s) 10, 53, 578
Tsp45I GTSAC 3 cut(s) 268, 439, 587
TspDTI ATGAA 5 cut(s) 68, 102, 147, 257, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.