Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003431

Physical Location & Seq

Genomic Coordinates Forward (+)

27126523 .. 27129489

Gene Structure 2967 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1198200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGCAGCACGACGAGGTTATATGGCAGGTTATCCGCCACAAGCACTGCAGCTACATGTCCAAAATTGAAACTGGGATTTTCTGTAGAAACCCCTATAACGTTACCGGGATTTGTAACCGGAGCTCCTGCCCTCTAGCTAATAGTCGTTACGCCACCATTCGCGACCATGACGGAGTCTTCTATCTTTATATGAAAACTATAGAGAGAGCTCATATGCCGAACAAATTATGGGAAAGAGTTAAGTTGCCGCGGAATTATGAGAAAGCACTTGAAATTATTGACAAGCATCTGATGTATTGGCCTAAGTTTCTCGTGCACAAAACAAAGCAACGGCTAACTAAAATGACTCAGATGCGGATACGCATGAGGAAGCTTGCTTTGAAAACAAGGGAGAAAATAATGACAACACCAAGGAAACAGATAAAGAGAGAGGCTAGAAGAGAGGAAAAGGCTGAAAGAGCTGCACTATTGGACCAGAGCATTGAGAAAGAACTATTAGAACGCCTTAACAAAAATGTTTATGGAGATATATACAACTATCCCTTTAAACAATACCAAAAAGTTCTTGATGGGGCAGAGGTAGAGACTGAAAGGGAAGAAGAAGAGGAGGAGGAGGAACCTGAGATAGAATATGTTGAAGGCTATGATGAACTTGAAGAGGAAGATGATATAGAAGATTTTGCTGGTCTTGCAATGGACAATTCGCATGCAGATGACGATAATGTTGGAAGCGATGAAGAGGCAGTAGTTGCTCGCAAGAGAGGGCGAAAGGAATCTGCCTTTTCTTCTAAAAAGTTTGAGAAAGATGAACCTGCTGTCAAATCGAAGAAAAAGCCGAGGGTGCTTGTTGAGGTGGAGCATGAAGATACTGGTGCTAGGCAAAAAGCAGTCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mak16 Ribosomal_L28e

Protein Analysis

299

Amino Acids

35.36

Weight (kDa)

6.36

Isoelectric Point (pI)

55.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribosomal_L28e	PF01778	6 - 118	6.6e-39	Ribosomal L28e protein family
Mak16	PF04874	138 - 226	1.4e-22	Mak16 protein C-terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23280
fragaria_vesca	FvH4_4g25580
malus_domestica	MD03G1198200.v1.1 MD11G1214400.v1.1
prunus_persica	Prupe.1G244800_v2.0.a1 Prupe.1G244800_v2.0.a1 Prupe.8G011700_v2.0.a1 Prupe.8G011700_v2.0.a1 Prupe.8G011700_v2.0.a1 Prupe.8G016700_v2.0.a1 Prupe.8G016700_v2.0.a1 Prupe.8G016700_v2.0.a1 Prupe.8G016700_v2.0.a1
pyrus_communis	pycom03g14980 pycom11g18880
rosa_chinensis	RchiOBHm_Chr4g0433011 RchiOBHm_Chr7g0190541
rosa_laevigata	RLG00000004517 RLG00000006793
rosa_multiflora	Rmu_sc0001762.1_g000037 Rmu_sc0001784.1_g000019
rosa_roxburghii	Rroxscaffold_3G00264520 Rroxscaffold_5G00374450
rosa_rugosa	Rorug04G0265000 Rorug04G0265000 Rorug06G0505000
rosa_samantha	Rh4AG318600 Rh4BG326700 Rh4CG342100 Rh4DG321900 Rh7AG111500 Rh7BG114500 Rh7CG116300 Rh7DG115400
rosa_wichuraiana	Rw4G027710 Rw7G009730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	16, 820
AccII	CGCG	2 cut(s)	162, 250
AciI	CCGC	4 cut(s)	34, 248, 250, 355
AclI	AACGTT	1 cut(s)	99
AflIII	ACRYGT	1 cut(s)	54
AgsI	TTSAA	5 cut(s)	68, 272, 382, 638, 656
AluBI	AGCT	6 cut(s)	51, 123, 137, 209, 373, 461
AluI	AGCT	6 cut(s)	51, 123, 137, 209, 373, 461
Alw21I	GWGCWC	3 cut(s)	125, 211, 318
Alw26I	GTCTC	1 cut(s)	578
Alw44I	GTGCAC	1 cut(s)	314
AoxI	GGCC	1 cut(s)	299
ApaLI	GTGCAC	1 cut(s)	314
ApeKI	GCWGC	3 cut(s)	4, 48, 461
AspS9I	GGNCC	1 cut(s)	472
AsuC2I	CCSGG	1 cut(s)	106
AvaII	GGWCC	1 cut(s)	472
BaeGI	GKGCMC	1 cut(s)	318
BanII	GRGCYC	2 cut(s)	125, 211
BauI	CACGAG	1 cut(s)	311
BbsI	GAAGAC	1 cut(s)	169
Bbv12I	GWGCWC	3 cut(s)	125, 211, 318
BbvI	GCAGC	3 cut(s)	16, 60, 448
BccI	CCATC	1 cut(s)	563
BceAI	ACGGC	1 cut(s)	347
BciVI	GTATCC	1 cut(s)	351
BcnI	CCSGG	1 cut(s)	106
BcoDI	GTCTC	1 cut(s)	578
BfaI	CTAG	3 cut(s)	134, 435, 876
BfmI	CTRYAG	3 cut(s)	46, 82, 198
BfuAI	ACCTGC	2 cut(s)	16, 820
BfuI	GTATCC	1 cut(s)	351
BisI	GCNGC	4 cut(s)	5, 49, 248, 462
BlsI	GCNGC	4 cut(s)	6, 50, 249, 463
Bme1390I	CCNGG	1 cut(s)	106
Bme18I	GGWCC	1 cut(s)	472
BmgT120I	GGNCC	1 cut(s)	472
BmiI	GGNNCC	1 cut(s)	618
BmrFI	CCNGG	1 cut(s)	106
BmrI	ACTGGG	1 cut(s)	81
BmsI	GCATC	2 cut(s)	295, 342
BmuI	ACTGGG	1 cut(s)	81
BpiI	GAAGAC	1 cut(s)	169
BpuMI	CCSGG	1 cut(s)	106
BsaJI	CCNNGG	3 cut(s)	248, 410, 836
BsaWI	WCCGGW	1 cut(s)	117
BsaXI	ACNNNNNCTCC	2 cut(s)	107, 137
Bse1I	ACTGG	3 cut(s)	76, 874, 892
Bse3DI	GCAATG	1 cut(s)	699
BseDI	CCNNGG	3 cut(s)	248, 410, 836
BseMI	GCAATG	1 cut(s)	699
BseMII	CTCAG	2 cut(s)	362, 612
BseNI	ACTGG	3 cut(s)	76, 874, 892
BseRI	GAGGAG	3 cut(s)	620, 623, 626
BseSI	GKGCMC	1 cut(s)	318
BseXI	GCAGC	3 cut(s)	16, 60, 448
BsgI	GTGCAG	1 cut(s)	447
Bsh1236I	CGCG	2 cut(s)	162, 250
BshFI	GGCC	1 cut(s)	301
BsiHKAI	GWGCWC	3 cut(s)	125, 211, 318
BsiSI	CCGG	2 cut(s)	105, 118
BsmAI	GTCTC	1 cut(s)	578
BsnI	GGCC	1 cut(s)	301
Bsp1286I	GDGCHC	3 cut(s)	125, 211, 318
Bsp68I	TCGCGA	1 cut(s)	162
BspACI	CCGC	4 cut(s)	34, 248, 250, 355
BspANI	GGCC	1 cut(s)	301
BspCNI	CTCAG	2 cut(s)	361, 613
BspFNI	CGCG	2 cut(s)	162, 250
BspLI	GGNNCC	1 cut(s)	618
BspMAI	CTGCAG	1 cut(s)	50
BspMI	ACCTGC	2 cut(s)	16, 820
BsrDI	GCAATG	1 cut(s)	699
BsrI	ACTGG	3 cut(s)	76, 874, 892
BssECI	CCNNGG	3 cut(s)	248, 410, 836
BssSI	CACGAG	1 cut(s)	311
BssT1I	CCWWGG	1 cut(s)	410
Bst2BI	CACGAG	1 cut(s)	311
Bst6I	CTCTTC	4 cut(s)	433, 597, 651, 732
BstAPI	GCANNNNNTGC	1 cut(s)	749
BstC8I	GCNNGC	3 cut(s)	375, 708, 754
BstDEI	CTNAG	3 cut(s)	303, 348, 621
BstDSI	CCRYGG	1 cut(s)	248
BstFNI	CGCG	2 cut(s)	162, 250
BstMAI	GTCTC	1 cut(s)	578
BstMWI	GCNNNNNNNGC	4 cut(s)	458, 689, 749, 841
BstNSI	RCATGY	2 cut(s)	58, 710
BstSCI	CCNGG	1 cut(s)	104
BstSFI	CTRYAG	3 cut(s)	46, 82, 198
BstSLI	GKGCMC	1 cut(s)	318
BstUI	CGCG	2 cut(s)	162, 250
BstV1I	GCAGC	3 cut(s)	16, 60, 448
BstV2I	GAAGAC	1 cut(s)	169
BsuI	GTATCC	1 cut(s)	351
BsuRI	GGCC	1 cut(s)	301
BtgI	CCRYGG	1 cut(s)	248
BtgZI	GCGATG	1 cut(s)	747
BtsI	GCAGTG	1 cut(s)	43
BtsIMutI	CAGTG	1 cut(s)	43
BtuMI	TCGCGA	1 cut(s)	162
BveI	ACCTGC	2 cut(s)	16, 820
Cac8I	GCNNGC	3 cut(s)	375, 708, 754
Cfr13I	GGNCC	1 cut(s)	472
Cfr42I	CCGCGG	1 cut(s)	251
CviAII	CATG	5 cut(s)	55, 167, 364, 707, 860
DdeI	CTNAG	3 cut(s)	303, 348, 621
DraI	TTTAAA	1 cut(s)	547
Eam1104I	CTCTTC	4 cut(s)	433, 597, 651, 732
EarI	CTCTTC	4 cut(s)	433, 597, 651, 732
EciI	GGCGGA	1 cut(s)	23
Ecl136II	GAGCTC	2 cut(s)	123, 209
Eco130I	CCWWGG	1 cut(s)	410
Eco24I	GRGCYC	2 cut(s)	125, 211
Eco47I	GGWCC	1 cut(s)	472
Eco53kI	GAGCTC	2 cut(s)	123, 209
EcoICRI	GAGCTC	2 cut(s)	123, 209
EcoT14I	CCWWGG	1 cut(s)	410
EcoT38I	GRGCYC	2 cut(s)	125, 211
ErhI	CCWWGG	1 cut(s)	410
FaeI	CATG	5 cut(s)	58, 170, 367, 710, 863
FatI	CATG	5 cut(s)	54, 166, 363, 706, 859
FauNDI	CATATG	1 cut(s)	213
Fnu4HI	GCNGC	4 cut(s)	5, 49, 248, 462
FriOI	GRGCYC	2 cut(s)	125, 211
Fsp4HI	GCNGC	4 cut(s)	5, 49, 248, 462
FspBI	CTAG	3 cut(s)	134, 435, 876
GluI	GCNGC	4 cut(s)	5, 49, 248, 462
HaeIII	GGCC	1 cut(s)	301
HapII	CCGG	2 cut(s)	105, 118
Hin1II	CATG	5 cut(s)	58, 170, 367, 710, 863
HindIII	AAGCTT	1 cut(s)	371
HinfI	GANTC	3 cut(s)	174, 346, 773
HpaII	CCGG	2 cut(s)	105, 118
Hpy166II	GTNNAC	1 cut(s)	316
Hpy188I	TCNGA	2 cut(s)	291, 351
Hpy188III	TCNNGA	2 cut(s)	161, 566
Hpy8I	GTNNAC	1 cut(s)	316
Hpy99I	CGWCG	1 cut(s)	14
HpyAV	CCTTC	1 cut(s)	632
HpyCH4IV	ACGT	1 cut(s)	99
HpyCH4V	TGCA	6 cut(s)	4, 48, 316, 464, 692, 710
HpyF10VI	GCNNNNNNNGC	4 cut(s)	458, 689, 749, 841
HpyF3I	CTNAG	3 cut(s)	303, 348, 621
HpySE526I	ACGT	1 cut(s)	99
Hsp92II	CATG	5 cut(s)	58, 170, 367, 710, 863
KspI	CCGCGG	1 cut(s)	251
LmnI	GCTCC	3 cut(s)	120, 128, 856
Lsp1109I	GCAGC	3 cut(s)	16, 60, 448
LweI	GCATC	2 cut(s)	295, 342
MaeI	CTAG	3 cut(s)	134, 435, 876
MaeII	ACGT	1 cut(s)	99
MaeIII	GTNAC	3 cut(s)	100, 113, 146
MhlI	GDGCHC	3 cut(s)	125, 211, 318
MluCI	AATT	5 cut(s)	63, 224, 253, 273, 700
MlyI	GAGTC	2 cut(s)	183, 340
MmeI	TCCRAC	1 cut(s)	706
MseI	TTAA	3 cut(s)	240, 507, 546
MslI	CAYNNNNRTG	1 cut(s)	711
MspA1I	CMGCKG	1 cut(s)	250
MspI	CCGG	2 cut(s)	105, 118
MspR9I	CCNGG	1 cut(s)	106
MvnI	CGCG	2 cut(s)	162, 250
MwoI	GCNNNNNNNGC	4 cut(s)	458, 689, 749, 841
NciI	CCSGG	1 cut(s)	106
NdeI	CATATG	1 cut(s)	213
NlaIII	CATG	5 cut(s)	58, 170, 367, 710, 863
NlaIV	GGNNCC	1 cut(s)	618
NmeAIII	GCCGAG	1 cut(s)	861
NruI	TCGCGA	1 cut(s)	162
NspI	RCATGY	2 cut(s)	58, 710
PaeI	GCATGC	1 cut(s)	710
PciI	ACATGT	1 cut(s)	54
PfeI	GAWTC	1 cut(s)	773
PflFI	GACNNNGTC	1 cut(s)	173
PkrI	GCNGC	4 cut(s)	6, 50, 249, 463
PleI	GAGTC	2 cut(s)	182, 340
PpsI	GAGTC	2 cut(s)	182, 340
PscI	ACATGT	1 cut(s)	54
Psp124BI	GAGCTC	2 cut(s)	125, 211
Psp1406I	AACGTT	1 cut(s)	99
PspN4I	GGNNCC	1 cut(s)	618
PspPI	GGNCC	1 cut(s)	472
PstI	CTGCAG	1 cut(s)	50
PsyI	GACNNNGTC	1 cut(s)	173
RruI	TCGCGA	1 cut(s)	162
RseI	CAYNNNNRTG	1 cut(s)	711
SacI	GAGCTC	2 cut(s)	125, 211
SacII	CCGCGG	1 cut(s)	251
SaqAI	TTAA	3 cut(s)	240, 507, 546
SatI	GCNGC	4 cut(s)	5, 49, 248, 462
Sau96I	GGNCC	1 cut(s)	472
SchI	GAGTC	2 cut(s)	183, 340
ScrFI	CCNGG	1 cut(s)	106
SduI	GDGCHC	3 cut(s)	125, 211, 318
SfaNI	GCATC	2 cut(s)	295, 342
SfcI	CTRYAG	3 cut(s)	46, 82, 198
Sfr303I	CCGCGG	1 cut(s)	251
SgrBI	CCGCGG	1 cut(s)	251
SinI	GGWCC	1 cut(s)	472
SmiMI	CAYNNNNRTG	1 cut(s)	711
SphI	GCATGC	1 cut(s)	710
Sse9I	AATT	5 cut(s)	63, 224, 253, 273, 700
SsiI	CCGC	4 cut(s)	34, 248, 250, 355
SspMI	CTAG	3 cut(s)	134, 435, 876
SstI	GAGCTC	2 cut(s)	125, 211
StyD4I	CCNGG	1 cut(s)	104
StyI	CCWWGG	1 cut(s)	410
TaiI	ACGT	1 cut(s)	102
TaqI	TCGA	1 cut(s)	824
TasI	AATT	5 cut(s)	63, 224, 253, 273, 700
TauI	GCSGC	1 cut(s)	250
TfiI	GAWTC	1 cut(s)	773
Tru1I	TTAA	3 cut(s)	240, 507, 546
Tru9I	TTAA	3 cut(s)	240, 507, 546
TscAI	CASTG	1 cut(s)	50
TseI	GCWGC	3 cut(s)	4, 48, 461
TspDTI	ATGAA	5 cut(s)	206, 663, 750, 822, 876
TspGWI	ACGGA	1 cut(s)	186
TspRI	CASTG	1 cut(s)	50
Tth111I	GACNNNGTC	1 cut(s)	173
VneI	GTGCAC	1 cut(s)	314
VpaK11BI	GGWCC	1 cut(s)	472
XceI	RCATGY	2 cut(s)	58, 710
XspI	CTAG	3 cut(s)	134, 435, 876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.