Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002345

Physical Location & Seq

Genomic Coordinates Forward (+)

31522822 .. 31527822

Gene Structure 5001 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1229900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGATTGTTTAAAGGCTGCTATTCATCATAAGGGTTCTTTGTGCTGCCAGCTATTATTATTCTTTATCTGGTTATCCAGTTTCCAAGATGTCGTGGCAGTGCAGACAGTATTTGACCCAAGTCATGCTTCTTCCGTGGCAGAGCTAGGCCTAGCTAATCCACCTACTACTGGGTTCTTTAATCCCATTGAAATATCACCAGCTGTTATTCCAAACTACCCAAATCCTGCAGATGAACCTTCACAACCGATGTACCCGAATTTTCCGACAAGATATGAGCCTGTTTTAACCGGGAAGTGTCCTGTGAATTTTTCTACCATATCAAGTGTAATAGAAAAGACAGCTACTGATTGCTTTCAACCTTTGGCGGCAATATTAGGAAATGTAATGTGTTGCCCGCAGTTTAGTAGTTTAATCCGCATCTTCCAGGGTCTCTATAGCTTCAACTCTGATAAGTTGGTTCTGCAAAATTCAGTCGCGAATGATTGTTTTAAAGATATCATCAGTATCTTAGCCAGTAGGGGTGCCAACAGTTCAGTACCTACACTCTGCTCCATAAATTCGTCAAATCTTACTGGTGGGTCCTGTCCAGTGAAGGACATTAATGCCTTTGAGAAAACAGTAAATACAAGCAGTTTAGTGGAGGCTTGCACAACTGTTGACCCTCTCAAAGAGTGCTGCAGACCAATTTGCCAGCATGCAATAGCGGATGCTGCACTAAGGATCTCAGGAAAACAATTGATGATGAATGAGAATAAAAATTTAGTGGGTGGGCTTAATTATACTGATACTCTAAGTGATTGTAAAGGTGTGGTATTTTCATATCTTTCAAGGAAACTCTCGTCAGATGCTGCCAATACTGCATTTCGAATATTATCTGCCTGCAAAGTTAACAAAGTTTGCCCTTTGGAATTTAAGCAGCCTTCAGAAGTAGTTAAATCATGTCGCAATGTTGCTGCCCCGAGTCCTTCCTGCTGTAGCTCATTAAACACTTATATTTCGGGAATACAAAAGCAAATGTTAATTACAAATAGACAAGCTATAATCTGTGCTTCTGTGTTTGGGTCCATGTTGAGAAAAGGCGGGGTGATGGAAAATGTTTATGAGCTTTGTGATATTGACTTGAAAGACTTTAGCATTCAAGAATACGTGCAGCAAGGGTGTTTACTTCGAAGCTTACCTGCGGATGCGATAATTGACAATACAACAGGTTTCAGCTTTTCATGTGATTTGAGTGACAACACTGCAGCGCCATGGCCTTCATCTTCCTCAGATTCATCGTTGTCCTTTTGTGCACCTGAGATGTCATTGCCTGCACTACCAACATCGGAGACTTTTAAAAATCCTGGCTGTCGTGGTGGTGAGCTGGAATTTATGGTACCCGTTTTTTCATTTTTTGTTTTGGGTACTCTGTTGTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

474

Amino Acids

51.09

Weight (kDa)

5.27

Isoelectric Point (pI)

46.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPARK	PF19160	97 - 249	9.8e-22	SPARK
At1g61900	PF26584	299 - 372	1.1e-32	At1g61900-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002345)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30700 AT2G30700
fragaria_vesca	FvH4_4g10670 FvH4_4g10670 FvH4_4g10670 FvH4_4g10670
malus_domestica	MD03G1229900.v1.1 MD11G1250400.v1.1
prunus_persica	Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.1G201300_v2.0.a1 Prupe.4G179400_v2.0.a1 Prupe.4G179400_v2.0.a1 Prupe.4G179400_v2.0.a1 Prupe.4G179400_v2.0.a1 Prupe.4G179400_v2.0.a1 Prupe.4G179400_v2.0.a1
pyrus_communis	pycom03g17750 pycom11g22160
rosa_chinensis	RchiOBHm_Chr4g0406321
rosa_laevigata	RLG00000008775
rosa_multiflora	Rmu_sc0003452.1_g000013 Rmu_sc0010810.1_g000001
rosa_roxburghii	Rroxscaffold_5G00351050
rosa_rugosa	Rorug04G0068700 Rorug04G0068800 Rorug04G0068900 Rorug04G0069000 Rorug04G0069100
rosa_samantha	Rh4AG137300 Rh4AG137500 Rh4BG134900 Rh4CG145500 Rh4DG131700
rosa_wichuraiana	Rw4G011110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1189
Acc65I	GGTACC	1 cut(s)	1378
AccB1I	GGYRCC	2 cut(s)	524, 1378
AccII	CGCG	1 cut(s)	479
AciI	CCGC	6 cut(s)	368, 398, 418, 707, 1083, 1184
AclWI	GGATC	1 cut(s)	731
AcsI	RAATTY	7 cut(s)	259, 307, 469, 559, 760, 911, 1370
AcuI	CTGAAG	1 cut(s)	909
AfaI	GTAC	5 cut(s)	254, 540, 1380, 1408, 1418
AfiI	CCNNNNNNNGG	1 cut(s)	170
AgsI	TTSAA	6 cut(s)	191, 359, 445, 831, 1126, 1142
AjnI	CCWGG	2 cut(s)	426, 1345
AjuI	GAANNNNNNNTTGG	2 cut(s)	112, 144
Alw21I	GWGCWC	1 cut(s)	1297
Alw26I	GTCTC	2 cut(s)	437, 1325
Alw44I	GTGCAC	1 cut(s)	1293
AlwI	GGATC	1 cut(s)	731
AlwNI	CAGNNNCTG	2 cut(s)	347, 851
Ama87I	CYCGRG	1 cut(s)	961
AoxI	GGCC	2 cut(s)	148, 1256
ApaLI	GTGCAC	1 cut(s)	1293
ApeKI	GCWGC	9 cut(s)	17, 45, 678, 713, 851, 919, 956, 1153, 1247
ApoI	RAATTY	7 cut(s)	259, 307, 469, 559, 760, 911, 1370
AseI	ATTAAT	1 cut(s)	603
Asp718I	GGTACC	1 cut(s)	1378
AspLEI	GCGC	1 cut(s)	1252
AspS9I	GGNCC	2 cut(s)	582, 1065
AsuC2I	CCSGG	1 cut(s)	292
AsuHPI	GGTGA	3 cut(s)	189, 1099, 1373
AsuII	TTCGAA	2 cut(s)	868, 1171
AvaI	CYCGRG	1 cut(s)	961
AvaII	GGWCC	2 cut(s)	582, 1065
BaeGI	GKGCMC	1 cut(s)	1297
BaeI	ACNNNNGTAYC	2 cut(s)	236, 269
BanI	GGYRCC	2 cut(s)	524, 1378
Bbv12I	GWGCWC	1 cut(s)	1297
BbvI	GCAGC	9 cut(s)	4, 32, 665, 700, 838, 931, 943, 1165, 1259
BccI	CCATC	1 cut(s)	1084
BciT130I	CCWGG	2 cut(s)	428, 1347
BcnI	CCSGG	1 cut(s)	292
BcoDI	GTCTC	2 cut(s)	437, 1325
BfaI	CTAG	2 cut(s)	146, 152
BfmI	CTRYAG	5 cut(s)	228, 436, 679, 976, 1245
BfoI	RGCGCY	1 cut(s)	1253
BfuAI	ACCTGC	1 cut(s)	1189
Bme1390I	CCNGG	3 cut(s)	292, 428, 1347
Bme18I	GGWCC	2 cut(s)	582, 1065
BmeT110I	CYCGRG	1 cut(s)	961
BmgT120I	GGNCC	2 cut(s)	582, 1065
BmiI	GGNNCC	4 cut(s)	526, 583, 1066, 1380
BmrFI	CCNGG	3 cut(s)	292, 428, 1347
BmrI	ACTGGG	1 cut(s)	180
BmsI	GCATC	4 cut(s)	429, 700, 838, 1177
BmuI	ACTGGG	1 cut(s)	180
BoxI	GACNNNNGTC	1 cut(s)	120
Bpu14I	TTCGAA	2 cut(s)	868, 1171
BpuMI	CCSGG	1 cut(s)	292
BsaAI	YACGTR	1 cut(s)	1150
BsaI	GGTCTC	1 cut(s)	437
BsaJI	CCNNGG	3 cut(s)	135, 427, 1253
Bsc4I	CCNNNNNNNGG	1 cut(s)	170
Bse1I	ACTGG	5 cut(s)	78, 175, 516, 580, 590
Bse3DI	GCAATG	2 cut(s)	955, 1307
BseBI	CCWGG	2 cut(s)	428, 1347
BseDI	CCNNGG	3 cut(s)	135, 427, 1253
BseGI	GGATG	2 cut(s)	715, 1192
BseLI	CCNNNNNNNGG	1 cut(s)	170
BseMI	GCAATG	2 cut(s)	955, 1307
BseMII	CTCAG	3 cut(s)	741, 1284, 1290
BseNI	ACTGG	5 cut(s)	78, 175, 516, 580, 590
BseSI	GKGCMC	1 cut(s)	1297
BseXI	GCAGC	9 cut(s)	4, 32, 665, 700, 838, 931, 943, 1165, 1259
BsgI	GTGCAG	4 cut(s)	122, 699, 1172, 1299
Bsh1236I	CGCG	1 cut(s)	479
BshFI	GGCC	2 cut(s)	150, 1258
BshNI	GGYRCC	2 cut(s)	524, 1378
BsiHKAI	GWGCWC	1 cut(s)	1297
BsiHKCI	CYCGRG	1 cut(s)	961
BsiSI	CCGG	1 cut(s)	291
BslI	CCNNNNNNNGG	1 cut(s)	170
BsmAI	GTCTC	2 cut(s)	437, 1325
BsmI	GAATGC	1 cut(s)	1137
BsnI	GGCC	2 cut(s)	150, 1258
Bso31I	GGTCTC	1 cut(s)	437
BsoBI	CYCGRG	1 cut(s)	961
Bsp119I	TTCGAA	2 cut(s)	868, 1171
Bsp1286I	GDGCHC	1 cut(s)	1297
Bsp143I	GATC	1 cut(s)	723
Bsp19I	CCATGG	1 cut(s)	1253
Bsp68I	TCGCGA	1 cut(s)	479
BspACI	CCGC	6 cut(s)	368, 398, 418, 707, 1083, 1184
BspANI	GGCC	2 cut(s)	150, 1258
BspCNI	CTCAG	3 cut(s)	740, 1283, 1291
BspFNI	CGCG	1 cut(s)	479
BspLI	GGNNCC	4 cut(s)	526, 583, 1066, 1380
BspMAI	CTGCAG	3 cut(s)	232, 683, 1249
BspMI	ACCTGC	1 cut(s)	1189
BspPI	GGATC	1 cut(s)	731
BspT104I	TTCGAA	2 cut(s)	868, 1171
BspT107I	GGYRCC	2 cut(s)	524, 1378
BspTNI	GGTCTC	1 cut(s)	437
BsrDI	GCAATG	2 cut(s)	955, 1307
BsrI	ACTGG	5 cut(s)	78, 175, 516, 580, 590
BssECI	CCNNGG	3 cut(s)	135, 427, 1253
BssMI	GATC	1 cut(s)	723
BssT1I	CCWWGG	1 cut(s)	1253
Bst2UI	CCWGG	2 cut(s)	428, 1347
Bst4CI	ACNGT	4 cut(s)	109, 533, 622, 658
BstBAI	YACGTR	1 cut(s)	1150
BstBI	TTCGAA	2 cut(s)	868, 1171
BstC8I	GCNNGC	7 cut(s)	50, 398, 649, 695, 699, 883, 1314
BstDEI	CTNAG	6 cut(s)	511, 719, 727, 794, 1270, 1299
BstDSI	CCRYGG	2 cut(s)	135, 1253
BstF5I	GGATG	2 cut(s)	715, 1192
BstFNI	CGCG	1 cut(s)	479
BstH2I	RGCGCY	1 cut(s)	1253
BstHHI	GCGC	1 cut(s)	1252
BstKTI	GATC	1 cut(s)	726
BstMAI	GTCTC	2 cut(s)	437, 1325
BstMBI	GATC	1 cut(s)	723
BstMWI	GCNNNNNNNGC	2 cut(s)	713, 860
BstNI	CCWGG	2 cut(s)	428, 1347
BstNSI	RCATGY	1 cut(s)	701
BstPAI	GACNNNNGTC	1 cut(s)	120
BstSCI	CCNGG	3 cut(s)	290, 426, 1345
BstSFI	CTRYAG	5 cut(s)	228, 436, 679, 976, 1245
BstSLI	GKGCMC	1 cut(s)	1297
BstUI	CGCG	1 cut(s)	479
BstV1I	GCAGC	9 cut(s)	4, 32, 665, 700, 838, 931, 943, 1165, 1259
BstX2I	RGATCY	1 cut(s)	723
BstYI	RGATCY	1 cut(s)	723
BsuRI	GGCC	2 cut(s)	150, 1258
BtgI	CCRYGG	2 cut(s)	135, 1253
BtsCI	GGATG	2 cut(s)	715, 1192
BtsI	GCAGTG	2 cut(s)	105, 1242
BtsIMutI	CAGTG	3 cut(s)	105, 597, 1242
BtuMI	TCGCGA	1 cut(s)	479
BveI	ACCTGC	1 cut(s)	1189
Cac8I	GCNNGC	7 cut(s)	50, 398, 649, 695, 699, 883, 1314
CaiI	CAGNNNCTG	2 cut(s)	347, 851
CfoI	GCGC	1 cut(s)	1252
Cfr13I	GGNCC	2 cut(s)	582, 1065
Csp6I	GTAC	5 cut(s)	253, 539, 1379, 1407, 1417
CviAII	CATG	6 cut(s)	125, 698, 942, 1069, 1224, 1254
CviQI	GTAC	5 cut(s)	253, 539, 1379, 1407, 1417
DdeI	CTNAG	6 cut(s)	511, 719, 727, 794, 1270, 1299
DpnI	GATC	1 cut(s)	725
DpnII	GATC	1 cut(s)	723
DraI	TTTAAA	3 cut(s)	12, 493, 1339
Eco130I	CCWWGG	1 cut(s)	1253
Eco147I	AGGCCT	1 cut(s)	150
Eco31I	GGTCTC	1 cut(s)	437
Eco32I	GATATC	1 cut(s)	499
Eco47I	GGWCC	2 cut(s)	582, 1065
Eco57I	CTGAAG	1 cut(s)	909
Eco88I	CYCGRG	1 cut(s)	961
EcoO109I	RGGNCCY	1 cut(s)	582
EcoRII	CCWGG	2 cut(s)	426, 1345
EcoRV	GATATC	1 cut(s)	499
EcoT14I	CCWWGG	1 cut(s)	1253
ErhI	CCWWGG	1 cut(s)	1253
FaeI	CATG	6 cut(s)	128, 701, 945, 1072, 1227, 1257
FalI	AAGNNNNNCTT	2 cut(s)	112, 144
FatI	CATG	6 cut(s)	124, 697, 941, 1068, 1223, 1253
FauI	CCCGC	2 cut(s)	405, 1076
FokI	GGATG	2 cut(s)	722, 1199
FspBI	CTAG	2 cut(s)	146, 152
GlaI	GCGC	1 cut(s)	1251
HaeII	RGCGCY	1 cut(s)	1253
HaeIII	GGCC	2 cut(s)	150, 1258
HapII	CCGG	1 cut(s)	291
HhaI	GCGC	1 cut(s)	1252
Hin1II	CATG	6 cut(s)	128, 701, 945, 1072, 1227, 1257
Hin6I	GCGC	1 cut(s)	1250
HinP1I	GCGC	1 cut(s)	1250
HincII	GTYRAC	2 cut(s)	661, 892
HindII	GTYRAC	2 cut(s)	661, 892
HindIII	AAGCTT	1 cut(s)	1174
HinfI	GANTC	2 cut(s)	964, 1274
HpaI	GTTAAC	1 cut(s)	892
HpaII	CCGG	1 cut(s)	291
HphI	GGTGA	3 cut(s)	189, 1099, 1373
Hpy166II	GTNNAC	4 cut(s)	661, 892, 1166, 1295
Hpy188I	TCNGA	6 cut(s)	267, 451, 847, 928, 1273, 1330
Hpy188III	TCNNGA	4 cut(s)	478, 729, 1002, 1142
Hpy8I	GTNNAC	4 cut(s)	661, 892, 1166, 1295
HpyAV	CCTTC	5 cut(s)	249, 589, 933, 978, 1269
HpyCH4III	ACNGT	4 cut(s)	109, 533, 622, 658
HpyCH4IV	ACGT	1 cut(s)	1149
HpyF10VI	GCNNNNNNNGC	2 cut(s)	713, 860
HpyF3I	CTNAG	6 cut(s)	511, 719, 727, 794, 1270, 1299
HpySE526I	ACGT	1 cut(s)	1149
Hsp92II	CATG	6 cut(s)	128, 701, 945, 1072, 1227, 1257
HspAI	GCGC	1 cut(s)	1250
KpnI	GGTACC	1 cut(s)	1382
KspAI	GTTAAC	1 cut(s)	892
Kzo9I	GATC	1 cut(s)	723
LmnI	GCTCC	1 cut(s)	557
Lsp1109I	GCAGC	9 cut(s)	4, 32, 665, 700, 838, 931, 943, 1165, 1259
LweI	GCATC	4 cut(s)	429, 700, 838, 1177
MaeI	CTAG	2 cut(s)	146, 152
MaeII	ACGT	1 cut(s)	1149
MaeIII	GTNAC	1 cut(s)	1235
MalI	GATC	1 cut(s)	725
MboI	GATC	1 cut(s)	723
MboII	GAAGA	3 cut(s)	123, 415, 1257
MfeI	CAATTG	1 cut(s)	737
MflI	RGATCY	1 cut(s)	723
MhlI	GDGCHC	1 cut(s)	1297
MlyI	GAGTC	1 cut(s)	973
MmeI	TCCRAC	1 cut(s)	290
MnlI	CCTC	3 cut(s)	637, 675, 1279
MspA1I	CMGCKG	1 cut(s)	203
MspI	CCGG	1 cut(s)	291
MspR9I	CCNGG	3 cut(s)	292, 428, 1347
MunI	CAATTG	1 cut(s)	737
Mva1269I	GAATGC	1 cut(s)	1137
MvaI	CCWGG	2 cut(s)	428, 1347
MvnI	CGCG	1 cut(s)	479
MwoI	GCNNNNNNNGC	2 cut(s)	713, 860
NciI	CCSGG	1 cut(s)	292
NcoI	CCATGG	1 cut(s)	1253
NdeII	GATC	1 cut(s)	723
NlaIII	CATG	6 cut(s)	128, 701, 945, 1072, 1227, 1257
NlaIV	GGNNCC	4 cut(s)	526, 583, 1066, 1380
NmuCI	GTSAC	1 cut(s)	1235
NruI	TCGCGA	1 cut(s)	479
NspI	RCATGY	1 cut(s)	701
NspV	TTCGAA	2 cut(s)	868, 1171
PaeI	GCATGC	1 cut(s)	701
PceI	AGGCCT	1 cut(s)	150
PctI	GAATGC	1 cut(s)	1137
PfeI	GAWTC	1 cut(s)	1274
PleI	GAGTC	1 cut(s)	972
PpsI	GAGTC	1 cut(s)	972
Ppu21I	YACGTR	1 cut(s)	1150
PpuMI	RGGWCCY	1 cut(s)	582
PshAI	GACNNNNGTC	1 cut(s)	120
PshBI	ATTAAT	1 cut(s)	603
Psp5II	RGGWCCY	1 cut(s)	582
Psp6I	CCWGG	2 cut(s)	426, 1345
PspGI	CCWGG	2 cut(s)	426, 1345
PspN4I	GGNNCC	4 cut(s)	526, 583, 1066, 1380
PspPI	GGNCC	2 cut(s)	582, 1065
PspPPI	RGGWCCY	1 cut(s)	582
PstI	CTGCAG	3 cut(s)	232, 683, 1249
PstNI	CAGNNNCTG	2 cut(s)	347, 851
PsuI	RGATCY	1 cut(s)	723
PvuII	CAGCTG	1 cut(s)	203
RruI	TCGCGA	1 cut(s)	479
RsaI	GTAC	5 cut(s)	254, 540, 1380, 1408, 1418
RsaNI	GTAC	5 cut(s)	253, 539, 1379, 1407, 1417
Sau3AI	GATC	1 cut(s)	723
Sau96I	GGNCC	2 cut(s)	582, 1065
SchI	GAGTC	1 cut(s)	973
ScrFI	CCNGG	3 cut(s)	292, 428, 1347
SduI	GDGCHC	1 cut(s)	1297
SfaNI	GCATC	4 cut(s)	429, 700, 838, 1177
SfcI	CTRYAG	5 cut(s)	228, 436, 679, 976, 1245
SfuI	TTCGAA	2 cut(s)	868, 1171
SinI	GGWCC	2 cut(s)	582, 1065
SphI	GCATGC	1 cut(s)	701
SseBI	AGGCCT	1 cut(s)	150
SsiI	CCGC	6 cut(s)	368, 398, 418, 707, 1083, 1184
SspI	AATATT	2 cut(s)	375, 873
SspMI	CTAG	2 cut(s)	146, 152
StuI	AGGCCT	1 cut(s)	150
StyD4I	CCNGG	3 cut(s)	290, 426, 1345
StyI	CCWWGG	1 cut(s)	1253
TaaI	ACNGT	4 cut(s)	109, 533, 622, 658
TaiI	ACGT	1 cut(s)	1152
TaqI	TCGA	2 cut(s)	868, 1171
TatI	WGTACW	1 cut(s)	1416
TauI	GCSGC	1 cut(s)	371
TfiI	GAWTC	1 cut(s)	1274
TscAI	CASTG	3 cut(s)	105, 597, 1249
TseFI	GTSAC	1 cut(s)	1235
TseI	GCWGC	9 cut(s)	17, 45, 678, 713, 851, 919, 956, 1153, 1247
Tsp45I	GTSAC	1 cut(s)	1235
TspDTI	ATGAA	8 cut(s)	14, 249, 761, 810, 1212, 1251, 1266, 1380
TspGWI	ACGGA	1 cut(s)	124
TspRI	CASTG	3 cut(s)	105, 597, 1249
VneI	GTGCAC	1 cut(s)	1293
VpaK11BI	GGWCC	2 cut(s)	582, 1065
VspI	ATTAAT	1 cut(s)	603
XapI	RAATTY	7 cut(s)	259, 307, 469, 559, 760, 911, 1370
XceI	RCATGY	1 cut(s)	701
XspI	CTAG	2 cut(s)	146, 152

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.