Rorug04G0068700

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002345
Physical Location & Seq
Reverse (-)
10715452 .. 10716650
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0068700.1

Sequence Viewer

Length: 1083 bp
ATGGTCCGAATTACCAAAGGAACTCAGTCAACATATCCTATCAACATCCACCCTCAAAACCATCATGCGCTCTTTAGTCCCTCTTGGTCTGCTGCAGTTGATGAAATCACCAAGTCACCATCCTTCACTTGGCTACCCGAAACAACGTGGCTAGTTCTTCCCCGAGATCTGAATAAAGGTGAGGCCATTGATGGGTCTAGGTTTTACAGTCTTAAAGGAAACTTTTACAACTCGAACATAAACATGCCCGAGGAGTCCAGAGATGCAGTTTGCCTTGGATCCTCTCACGGTTGGCTAGTTTTGTTGAACAACCAGCTCGAGTTTATGATCTCAACTCCATTCTTATCTGGAATTCAACTTCCCCTGCCACCACTCAAGACTTTTCCAGATATACTTGGCATTACATATCAGAAAGCATTTGGCGAGGCTGGGCTGTCTTCACGTCCGACATGCAGCAACAAAGACAAGATTGGAGTCATGGTGATGTACATCTTTGAAGACAGAGATAATTGCAGGTACGACAGCCTTGCTTTCTGCACAACATCTGATTATAAGTGGACAAAACTTGAGGAGCGCAGATGGTACAAAGAAATCATGGGGTGTGGTAATGAGTTCTATGTCTTAAAGGAAGACTATAGTGTTGATGTCTGGGATTTCGGCACATCTTCCATTCCCATGAAGAAGATGGAAATCAAACCTGATGCATGTGCAATACTTGACGAAGTAAGAGGAAGGTCTCCCAAGGTTTACATGATCAAAAGAGTGGGGACTGGTCTAATGCTGGTAGTGGAATATGTTCATATCTCACAAATTCAATTCCGTGTTTATAAGCTGGAGGAGACAACCCCTGTGTGGCAGGAGGTGCAATCGATTGGGAGTAATGCCTTGGTTTTAGGTTCGAACAATTCCATAGTAATGTCATTCCATGATTCCCTACGCTGTGATGATGATTCGATTTACTATGCGCAGAAGTCAAGACCTGAACCCTATAGTTTACATGTGTTTAGTTTGAAAGACAATTCAAGGAAAAAAGTTTGTGAACTGCCAGTGGACTTTGAACCACTAGCTTTTTCGGGATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

41.05

Weight (kDa)

5.87

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 68 - 335 9.4e-35 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 552, 828
Acc16I TGCGCA 1 cut(s) 966
Acc36I ACCTGC 1 cut(s) 504
AclWI GGATC 3 cut(s) 273, 286, 1071
AcsI RAATTY 2 cut(s) 351, 810
AfaI GTAC 3 cut(s) 488, 518, 584
AfiI CCNNNNNNNGG 3 cut(s) 129, 192, 852
AflIII ACRYGT 1 cut(s) 997
AgsI TTSAA 7 cut(s) 307, 356, 497, 815, 1012, 1023, 1058
AjiI CACGTC 1 cut(s) 443
AluBI AGCT 3 cut(s) 316, 832, 1067
AluI AGCT 3 cut(s) 316, 832, 1067
Alw26I GTCTC 2 cut(s) 741, 833
AlwI GGATC 3 cut(s) 273, 286, 1071
Ama87I CYCGRG 3 cut(s) 162, 248, 317
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 2 cut(s) 92, 453
ApoI RAATTY 2 cut(s) 351, 810
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 3 cut(s) 70, 576, 967
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 4 cut(s) 100, 108, 191, 493
AsuII TTCGAA 1 cut(s) 899
AvaI CYCGRG 3 cut(s) 162, 248, 317
AvaII GGWCC 1 cut(s) 4
BamHI GGATCC 2 cut(s) 278, 1076
BbsI GAAGAC 3 cut(s) 429, 504, 636
BbvI GCAGC 2 cut(s) 79, 465
BccI CCATC 5 cut(s) 69, 127, 185, 573, 679
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BclI TGATCA 1 cut(s) 753
BcoDI GTCTC 2 cut(s) 741, 833
BfaI CTAG 4 cut(s) 152, 198, 296, 1064
BfmI CTRYAG 3 cut(s) 93, 634, 988
BfuAI ACCTGC 1 cut(s) 504
BglII AGATCT 1 cut(s) 166
BisI GCNGC 2 cut(s) 93, 454
BlsI GCNGC 2 cut(s) 94, 455
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 3 cut(s) 162, 248, 317
BmgBI CACGTC 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 280, 1078
BmsI GCATC 2 cut(s) 253, 691
BpiI GAAGAC 3 cut(s) 429, 504, 636
BpmI CTGGAG 1 cut(s) 854
Bpu14I TTCGAA 1 cut(s) 899
BpuEI CTTGAG 2 cut(s) 359, 587
Bsa29I ATCGAT 1 cut(s) 869
BsaBI GATNNNNATC 2 cut(s) 488, 689
BsaI GGTCTC 1 cut(s) 741
BsaJI CCNNGG 4 cut(s) 249, 274, 741, 885
BsaXI ACNNNNNCTCC 2 cut(s) 465, 495
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 192, 852
Bse1I ACTGG 2 cut(s) 775, 1046
Bse8I GATNNNNATC 2 cut(s) 488, 689
BseCI ATCGAT 1 cut(s) 869
BseDI CCNNGG 4 cut(s) 249, 274, 741, 885
BseGI GGATG 2 cut(s) 45, 119
BseJI GATNNNNATC 2 cut(s) 488, 689
BseLI CCNNNNNNNGG 3 cut(s) 129, 192, 852
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 2 cut(s) 775, 1046
BseRI GAGGAG 3 cut(s) 266, 584, 851
BseXI GCAGC 2 cut(s) 79, 465
BseYI CCCAGC 1 cut(s) 428
BsgI GTGCAG 1 cut(s) 520
BshFI GGCC 1 cut(s) 185
BshVI ATCGAT 1 cut(s) 869
BsiHKCI CYCGRG 3 cut(s) 162, 248, 317
BslFI GGGAC 2 cut(s) 63, 781
BslI CCNNNNNNNGG 3 cut(s) 129, 192, 852
BsmAI GTCTC 2 cut(s) 741, 833
BsmFI GGGAC 2 cut(s) 63, 781
BsnI GGCC 1 cut(s) 185
Bso31I GGTCTC 1 cut(s) 741
BsoBI CYCGRG 3 cut(s) 162, 248, 317
Bsp119I TTCGAA 1 cut(s) 899
Bsp1407I TGTACA 1 cut(s) 486
Bsp143I GATC 5 cut(s) 166, 278, 327, 753, 1076
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 1 cut(s) 37
BspDI ATCGAT 1 cut(s) 869
BspLI GGNNCC 2 cut(s) 280, 1078
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 1 cut(s) 504
BspPI GGATC 3 cut(s) 273, 286, 1071
BspT104I TTCGAA 1 cut(s) 899
BspTNI GGTCTC 1 cut(s) 741
BsrGI TGTACA 1 cut(s) 486
BsrI ACTGG 2 cut(s) 775, 1046
BssECI CCNNGG 4 cut(s) 249, 274, 741, 885
BssMI GATC 5 cut(s) 166, 278, 327, 753, 1076
BssT1I CCWWGG 3 cut(s) 274, 741, 885
Bst4CI ACNGT 2 cut(s) 209, 290
BstAPI GCANNNNNTGC 1 cut(s) 862
BstAUI TGTACA 1 cut(s) 486
BstBI TTCGAA 1 cut(s) 899
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 45, 119
BstHHI GCGC 3 cut(s) 70, 576, 967
BstKTI GATC 5 cut(s) 169, 281, 330, 756, 1079
BstMAI GTCTC 2 cut(s) 741, 833
BstMBI GATC 5 cut(s) 166, 278, 327, 753, 1076
BstMWI GCNNNNNNNGC 1 cut(s) 862
BstNSI RCATGY 4 cut(s) 247, 453, 708, 1001
BstSFI CTRYAG 3 cut(s) 93, 634, 988
BstV1I GCAGC 2 cut(s) 79, 465
BstV2I GAAGAC 3 cut(s) 429, 504, 636
BstX2I RGATCY 3 cut(s) 166, 278, 1076
BstYI RGATCY 3 cut(s) 166, 278, 1076
Bsu15I ATCGAT 1 cut(s) 869
BsuRI GGCC 1 cut(s) 185
BsuTUI ATCGAT 1 cut(s) 869
BtrI CACGTC 1 cut(s) 443
BtsCI GGATG 2 cut(s) 45, 119
BtsIMutI CAGTG 1 cut(s) 1053
BveI ACCTGC 1 cut(s) 504
CfoI GCGC 3 cut(s) 70, 576, 967
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 869
Csp6I GTAC 3 cut(s) 487, 517, 583
CviQI GTAC 3 cut(s) 487, 517, 583
DdeI CTNAG 1 cut(s) 24
DpnI GATC 5 cut(s) 168, 280, 329, 755, 1078
DpnII GATC 5 cut(s) 166, 278, 327, 753, 1076
Eco130I CCWWGG 3 cut(s) 274, 741, 885
Eco31I GGTCTC 1 cut(s) 741
Eco47I GGWCC 1 cut(s) 4
Eco88I CYCGRG 3 cut(s) 162, 248, 317
EcoRI GAATTC 1 cut(s) 351
EcoT14I CCWWGG 3 cut(s) 274, 741, 885
EcoT22I ATGCAT 1 cut(s) 706
ErhI CCWWGG 3 cut(s) 274, 741, 885
FaqI GGGAC 2 cut(s) 63, 781
FbaI TGATCA 1 cut(s) 753
Fnu4HI GCNGC 2 cut(s) 93, 454
FokI GGATG 2 cut(s) 32, 106
Fsp4HI GCNGC 2 cut(s) 93, 454
FspBI CTAG 4 cut(s) 152, 198, 296, 1064
FspI TGCGCA 1 cut(s) 966
GlaI GCGC 3 cut(s) 69, 575, 966
GluI GCNGC 2 cut(s) 93, 454
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 1 cut(s) 854
HaeIII GGCC 1 cut(s) 185
HhaI GCGC 3 cut(s) 70, 576, 967
Hin6I GCGC 3 cut(s) 68, 574, 965
HinP1I GCGC 3 cut(s) 68, 574, 965
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 4 cut(s) 254, 474, 929, 950
HphI GGTGA 4 cut(s) 100, 108, 191, 493
Hpy166II GTNNAC 6 cut(s) 30, 558, 748, 995, 1040, 1051
Hpy188I TCNGA 5 cut(s) 8, 171, 411, 447, 547
Hpy188III TCNNGA 6 cut(s) 258, 348, 376, 386, 975, 1074
Hpy8I GTNNAC 6 cut(s) 30, 558, 748, 995, 1040, 1051
HpyAV CCTTC 2 cut(s) 133, 726
HpyCH4III ACNGT 2 cut(s) 209, 290
HpyCH4IV ACGT 2 cut(s) 146, 442
HpyCH4V TGCA 8 cut(s) 95, 266, 453, 513, 537, 704, 710, 865
HpyF10VI GCNNNNNNNGC 1 cut(s) 862
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 2 cut(s) 146, 442
HspAI GCGC 3 cut(s) 68, 574, 965
Ksp22I TGATCA 1 cut(s) 753
Kzo9I GATC 5 cut(s) 166, 278, 327, 753, 1076
LmnI GCTCC 1 cut(s) 571
Lsp1109I GCAGC 2 cut(s) 79, 465
LweI GCATC 2 cut(s) 253, 691
MaeI CTAG 4 cut(s) 152, 198, 296, 1064
MaeII ACGT 2 cut(s) 146, 442
MaeIII GTNAC 1 cut(s) 114
MalI GATC 5 cut(s) 168, 280, 329, 755, 1078
MboI GATC 5 cut(s) 166, 278, 327, 753, 1076
MboII GAAGA 7 cut(s) 149, 429, 509, 641, 657, 691, 694
MflI RGATCY 3 cut(s) 166, 278, 1076
MluCI AATT 7 cut(s) 9, 351, 508, 810, 815, 904, 1018
MlyI GAGTC 2 cut(s) 263, 483
MmeI TCCRAC 1 cut(s) 470
Mph1103I ATGCAT 1 cut(s) 706
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 2 cut(s) 213, 623
MslI CAYNNNNRTG 4 cut(s) 242, 482, 674, 914
MwoI GCNNNNNNNGC 1 cut(s) 862
NdeII GATC 5 cut(s) 166, 278, 327, 753, 1076
NlaIV GGNNCC 2 cut(s) 280, 1078
NmuCI GTSAC 1 cut(s) 114
NsbI TGCGCA 1 cut(s) 966
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 4 cut(s) 247, 453, 708, 1001
NspV TTCGAA 1 cut(s) 899
PaeR7I CTCGAG 1 cut(s) 317
PciI ACATGT 1 cut(s) 997
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 929, 950
PkrI GCNGC 2 cut(s) 94, 455
PleI GAGTC 2 cut(s) 262, 482
PpsI GAGTC 2 cut(s) 262, 482
PscI ACATGT 1 cut(s) 997
PsiI TTATAA 2 cut(s) 552, 828
PspFI CCCAGC 1 cut(s) 428
PspN4I GGNNCC 2 cut(s) 280, 1078
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 317
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 3 cut(s) 166, 278, 1076
RsaI GTAC 3 cut(s) 488, 518, 584
RsaNI GTAC 3 cut(s) 487, 517, 583
RseI CAYNNNNRTG 4 cut(s) 242, 482, 674, 914
SaqAI TTAA 2 cut(s) 213, 623
SatI GCNGC 2 cut(s) 93, 454
Sau3AI GATC 5 cut(s) 166, 278, 327, 753, 1076
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 263, 483
SfaNI GCATC 2 cut(s) 253, 691
SfcI CTRYAG 3 cut(s) 93, 634, 988
Sfr274I CTCGAG 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 899
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 4 cut(s) 242, 482, 674, 914
SmlI CTYRAG 3 cut(s) 317, 374, 566
SmoI CTYRAG 3 cut(s) 317, 374, 566
Sse9I AATT 7 cut(s) 9, 351, 508, 810, 815, 904, 1018
SspMI CTAG 4 cut(s) 152, 198, 296, 1064
StyI CCWWGG 3 cut(s) 274, 741, 885
TaaI ACNGT 2 cut(s) 209, 290
TaiI ACGT 2 cut(s) 149, 445
TaqI TCGA 5 cut(s) 233, 318, 869, 899, 953
TasI AATT 7 cut(s) 9, 351, 508, 810, 815, 904, 1018
TatI WGTACW 1 cut(s) 486
TfiI GAWTC 2 cut(s) 929, 950
Tru1I TTAA 2 cut(s) 213, 623
Tru9I TTAA 2 cut(s) 213, 623
TscAI CASTG 1 cut(s) 1053
TseFI GTSAC 1 cut(s) 114
TseI GCWGC 2 cut(s) 92, 453
Tsp45I GTSAC 1 cut(s) 114
TspDTI ATGAA 3 cut(s) 117, 692, 788
TspGWI ACGGA 1 cut(s) 809
TspRI CASTG 1 cut(s) 1053
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 351, 810
XceI RCATGY 4 cut(s) 247, 453, 708, 1001
XcmI CCANNNNNNNNNTGG 2 cut(s) 126, 682
XhoI CTCGAG 1 cut(s) 317
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 4 cut(s) 152, 198, 296, 1064
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.