Rh4BG134900

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002345
Physical Location & Seq
Reverse (-)
22575409 .. 22581681
6273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG134900.1

Sequence Viewer

Length: 1284 bp
ATGGACTGTTGTCAGACTCTTAGTCATCTAAAAGGTTCTTTGTGCTGTCAGTTATTATTATTCTTTATCTGGTTATCCAGTTTTCAAGATGCTGTGACATTGGAGACGGTCTTTGAGCAAAGTCATGTTCCTCAAATGCCAGAGCTTGCTAAACCACCTTCTAGTGGAATCTTTGGTCCTATAGAAATATCACCTGCAGTTATTCCACATTATCCAGATCCTGACGAACCCTTTCCGCCGATGTACCCTACATTTCCAACCAGATATGAGCCTGTTTTAACTGGGAAATGTCCTGTCAATTTCTCCGCTATATCGAGTATCATTGAAAAAACAGCATCTGATTGTTCACAACCTTTGGCAGCACTTGTAGGAAATGTAATATGTTGTCCACAGTTCAGTAGTTTGATCCGCATCTTCCAGGGTCTCTACAGCTTCAACTCGGCTAAGTTGGTTTTGCAAGATTCAGTTGCAAGTGATTGTTTTACAGATATAATCAGTATCTTAGCCAGTAGGGGTGCAAACAGTACAATACCTACACTTTGCTCTGTAAAATCTTCAAATCTTACTGGTGGGTCCTGTCCAGTGAAGGATGTTGATACCTTTGAAAAAACAGTAAACACAAGCAGATTACTGGAGGCCTGCATAGCTGTTGATCCTCTTAAAGAGTGCTGCCGACCAATTTGTCAACCTGCAATCATGGATGCTGCGCTTCAGATCTCCGGGAAGCAACTGCAGTTGGATGAGAATAAAAATTTGGTTGGTGAGCCGAATCATATCGACACTCCAAGTGACTGTAAAGGAGTGGTTTTTGCATATCTTTCAAGGAAACTCTCGTCAGAAGTTGTAAATACTGCATTTCGAATATTATCTGCCTGCAAAGTTAATAAAGTTTGTCCTTTGGATTTTAAGGAGCCTTCTGAAGTAATTAAAGCATGCCGCAATGTTGCTGCCCCAAGTCCTTCCTGCTGTAGCTCATTAAACACCTACATTGCAGGAATACAGAAGCAAATGTTGATTACAAACAGGCAAGCTATAATCTGTGCTACAGTGTTTGGTTCTATCTTAAGGAAAGGCGGGGTAATGACAAATATTTACGAGCTTTGTGATGTTGACTTAAAAGACTTTAGCATTCAAGGTATATATCCCGCTTGCCATTCATGTGTAAATTCTACCTTTTGTTCCATTATTGCATCTGATCTATCCTTCTTTTCTGGATATCTATGGGCACTGGAGCAGCATATGGTCAACAAGGTATGCCATTGTTATGTTTTATACTTTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

46.56

Weight (kDa)

5.68

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPARK PF19160 94 - 248 8.8e-23 SPARK
At1g61900 PF26584 296 - 369 6e-32 At1g61900-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 202
AasI GACNNNNNNGTC 1 cut(s) 681
Acc36I ACCTGC 2 cut(s) 202, 697
AciI CCGC 6 cut(s) 236, 306, 409, 937, 1074, 1146
AclWI GGATC 3 cut(s) 212, 400, 647
AcsI RAATTY 2 cut(s) 751, 1165
AcuI CTGAAG 2 cut(s) 695, 939
AfaI GTAC 2 cut(s) 245, 526
AfiI CCNNNNNNNGG 1 cut(s) 164
AflII CTTAAG 1 cut(s) 1063
AgsI TTSAA 7 cut(s) 86, 326, 436, 558, 605, 822, 1133
AhdI GACNNNNNGTC 1 cut(s) 21
AjnI CCWGG 1 cut(s) 417
AjuI GAANNNNNNNTTGG 2 cut(s) 737, 769
AluBI AGCT 6 cut(s) 145, 432, 647, 972, 1031, 1099
AluI AGCT 6 cut(s) 145, 432, 647, 972, 1031, 1099
Alw26I GTCTC 2 cut(s) 98, 428
AlwI GGATC 3 cut(s) 212, 400, 647
AlwNI CAGNNNCTG 2 cut(s) 221, 338
AoxI GGCC 1 cut(s) 636
ApeKI GCWGC 5 cut(s) 359, 669, 704, 947, 1234
ApoI RAATTY 2 cut(s) 751, 1165
Asp700I GAANNNNTTC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 2 cut(s) 176, 573
AsuC2I CCSGG 1 cut(s) 721
AsuHPI GGTGA 2 cut(s) 183, 773
AsuII TTCGAA 1 cut(s) 859
AvaII GGWCC 2 cut(s) 176, 573
BaeGI GKGCMC 1 cut(s) 1228
BbvI GCAGC 5 cut(s) 371, 656, 691, 934, 1246
BciT130I CCWGG 1 cut(s) 419
BcnI CCSGG 1 cut(s) 721
BcoDI GTCTC 2 cut(s) 98, 428
BfaI CTAG 2 cut(s) 162, 1282
BfmI CTRYAG 6 cut(s) 180, 195, 427, 731, 967, 1044
BfrI CTTAAG 1 cut(s) 1063
BfuAI ACCTGC 2 cut(s) 202, 697
BglII AGATCT 1 cut(s) 714
BisI GCNGC 6 cut(s) 360, 670, 705, 937, 948, 1235
BlsI GCNGC 6 cut(s) 361, 671, 706, 938, 949, 1236
Bme1390I CCNGG 2 cut(s) 419, 721
Bme18I GGWCC 2 cut(s) 176, 573
BmeRI GACNNNNNGTC 1 cut(s) 21
BmgT120I GGNCC 2 cut(s) 176, 573
BmiI GGNNCC 2 cut(s) 574, 912
BmrFI CCNGG 2 cut(s) 419, 721
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 5 cut(s) 79, 344, 420, 691, 1199
BmuI ACTGGG 1 cut(s) 291
BoxI GACNNNNGTC 1 cut(s) 9
BpmI CTGGAG 2 cut(s) 653, 1250
Bpu14I TTCGAA 1 cut(s) 859
BpuMI CCSGG 1 cut(s) 721
BsaBI GATNNNNATC 1 cut(s) 410
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 418
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse1I ACTGG 7 cut(s) 78, 286, 507, 571, 581, 636, 1233
Bse3DI GCAATG 2 cut(s) 946, 987
Bse8I GATNNNNATC 1 cut(s) 410
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 3 cut(s) 595, 706, 745
BseJI GATNNNNATC 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 2 cut(s) 946, 987
BseNI ACTGG 7 cut(s) 78, 286, 507, 571, 581, 636, 1233
BseSI GKGCMC 1 cut(s) 1228
BseXI GCAGC 5 cut(s) 371, 656, 691, 934, 1246
BshFI GGCC 1 cut(s) 638
BsiSI CCGG 1 cut(s) 720
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 2 cut(s) 98, 428
BsmBI CGTCTC 1 cut(s) 98
BsmI GAATGC 1 cut(s) 1128
BsnI GGCC 1 cut(s) 638
Bso31I GGTCTC 1 cut(s) 428
Bsp119I TTCGAA 1 cut(s) 859
Bsp1286I GDGCHC 1 cut(s) 1228
Bsp143I GATC 5 cut(s) 217, 405, 652, 714, 1195
BspACI CCGC 6 cut(s) 236, 306, 409, 937, 1074, 1146
BspANI GGCC 1 cut(s) 638
BspLI GGNNCC 2 cut(s) 574, 912
BspMAI CTGCAG 2 cut(s) 199, 735
BspMI ACCTGC 2 cut(s) 202, 697
BspPI GGATC 3 cut(s) 212, 400, 647
BspT104I TTCGAA 1 cut(s) 859
BspTI CTTAAG 1 cut(s) 1063
BspTNI GGTCTC 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 946, 987
BsrI ACTGG 7 cut(s) 78, 286, 507, 571, 581, 636, 1233
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 5 cut(s) 217, 405, 652, 714, 1195
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 7 cut(s) 8, 109, 393, 524, 613, 794, 1048
BstAFI CTTAAG 1 cut(s) 1063
BstBI TTCGAA 1 cut(s) 859
BstC8I GCNNGC 6 cut(s) 147, 640, 874, 934, 1029, 1150
BstDEI CTNAG 3 cut(s) 20, 444, 502
BstF5I GGATG 3 cut(s) 595, 706, 745
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 5 cut(s) 220, 408, 655, 717, 1198
BstMAI GTCTC 2 cut(s) 98, 428
BstMBI GATC 5 cut(s) 217, 405, 652, 714, 1195
BstMWI GCNNNNNNNGC 1 cut(s) 644
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 1 cut(s) 936
BstPAI GACNNNNGTC 1 cut(s) 9
BstSCI CCNGG 2 cut(s) 417, 719
BstSFI CTRYAG 6 cut(s) 180, 195, 427, 731, 967, 1044
BstSLI GKGCMC 1 cut(s) 1228
BstV1I GCAGC 5 cut(s) 371, 656, 691, 934, 1246
BstX2I RGATCY 2 cut(s) 217, 714
BstYI RGATCY 2 cut(s) 217, 714
BsuRI GGCC 1 cut(s) 638
BtsCI GGATG 3 cut(s) 595, 706, 745
BtsIMutI CAGTG 3 cut(s) 588, 1053, 1226
BveI ACCTGC 2 cut(s) 202, 697
Cac8I GCNNGC 6 cut(s) 147, 640, 874, 934, 1029, 1150
CaiI CAGNNNCTG 2 cut(s) 221, 338
CfoI GCGC 1 cut(s) 709
Cfr13I GGNCC 2 cut(s) 176, 573
Csp6I GTAC 2 cut(s) 244, 525
CviAII CATG 4 cut(s) 125, 697, 933, 1158
CviQI GTAC 2 cut(s) 244, 525
DdeI CTNAG 3 cut(s) 20, 444, 502
DpnI GATC 5 cut(s) 219, 407, 654, 716, 1197
DpnII GATC 5 cut(s) 217, 405, 652, 714, 1195
DrdI GACNNNNNNGTC 1 cut(s) 681
DriI GACNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 681
Eam1105I GACNNNNNGTC 1 cut(s) 21
EciI GGCGGA 1 cut(s) 225
Eco147I AGGCCT 1 cut(s) 638
Eco31I GGTCTC 1 cut(s) 428
Eco32I GATATC 1 cut(s) 1217
Eco47I GGWCC 2 cut(s) 176, 573
Eco57I CTGAAG 2 cut(s) 695, 939
EcoO109I RGGNCCY 1 cut(s) 573
EcoRII CCWGG 1 cut(s) 417
EcoRV GATATC 1 cut(s) 1217
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 4 cut(s) 128, 700, 936, 1161
FatI CATG 4 cut(s) 124, 696, 932, 1157
FauI CCCGC 2 cut(s) 1067, 1153
FauNDI CATATG 1 cut(s) 1239
Fnu4HI GCNGC 6 cut(s) 360, 670, 705, 937, 948, 1235
FokI GGATG 3 cut(s) 602, 713, 752
Fsp4HI GCNGC 6 cut(s) 360, 670, 705, 937, 948, 1235
FspBI CTAG 2 cut(s) 162, 1282
GlaI GCGC 1 cut(s) 708
GluI GCNGC 6 cut(s) 360, 670, 705, 937, 948, 1235
GsuI CTGGAG 2 cut(s) 653, 1250
HaeIII GGCC 1 cut(s) 638
HapII CCGG 1 cut(s) 720
HhaI GCGC 1 cut(s) 709
Hin1II CATG 4 cut(s) 128, 700, 936, 1161
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HincII GTYRAC 3 cut(s) 686, 1111, 1246
HindII GTYRAC 3 cut(s) 686, 1111, 1246
HinfI GANTC 4 cut(s) 16, 168, 461, 769
HpaII CCGG 1 cut(s) 720
HphI GGTGA 2 cut(s) 183, 773
Hpy166II GTNNAC 6 cut(s) 347, 389, 616, 686, 1111, 1246
Hpy188I TCNGA 6 cut(s) 15, 340, 714, 838, 919, 1195
Hpy188III TCNNGA 4 cut(s) 86, 215, 221, 1212
Hpy8I GTNNAC 6 cut(s) 347, 389, 616, 686, 1111, 1246
HpyAV CCTTC 5 cut(s) 168, 580, 924, 969, 1213
HpyCH4III ACNGT 7 cut(s) 8, 109, 393, 524, 613, 794, 1048
HpyF10VI GCNNNNNNNGC 1 cut(s) 644
HpyF3I CTNAG 3 cut(s) 20, 444, 502
Hsp92II CATG 4 cut(s) 128, 700, 936, 1161
HspAI GCGC 1 cut(s) 707
Kzo9I GATC 5 cut(s) 217, 405, 652, 714, 1195
LmnI GCTCC 2 cut(s) 910, 1231
Lsp1109I GCAGC 5 cut(s) 371, 656, 691, 934, 1246
LweI GCATC 5 cut(s) 79, 344, 420, 691, 1199
MaeI CTAG 2 cut(s) 162, 1282
MaeIII GTNAC 2 cut(s) 94, 788
MalI GATC 5 cut(s) 219, 407, 654, 716, 1197
MboI GATC 5 cut(s) 217, 405, 652, 714, 1195
MboII GAAGA 2 cut(s) 406, 546
MflI RGATCY 2 cut(s) 217, 714
MhlI GDGCHC 1 cut(s) 1228
MluCI AATT 5 cut(s) 298, 678, 751, 924, 1165
MlyI GAGTC 1 cut(s) 10
MmeI TCCRAC 2 cut(s) 281, 717
MnlI CCTC 3 cut(s) 141, 628, 666
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 8 cut(s) 278, 660, 882, 906, 927, 977, 1064, 1115
MslI CAYNNNNRTG 2 cut(s) 1158, 1263
MspCI CTTAAG 1 cut(s) 1063
MspI CCGG 1 cut(s) 720
MspR9I CCNGG 2 cut(s) 419, 721
Mva1269I GAATGC 1 cut(s) 1128
MvaI CCWGG 1 cut(s) 419
MwoI GCNNNNNNNGC 1 cut(s) 644
NciI CCSGG 1 cut(s) 721
NdeI CATATG 1 cut(s) 1239
NdeII GATC 5 cut(s) 217, 405, 652, 714, 1195
NlaIII CATG 4 cut(s) 128, 700, 936, 1161
NlaIV GGNNCC 2 cut(s) 574, 912
NmeAIII GCCGAG 1 cut(s) 419
NmuCI GTSAC 2 cut(s) 94, 788
NspI RCATGY 1 cut(s) 936
NspV TTCGAA 1 cut(s) 859
PaeI GCATGC 1 cut(s) 936
PaqCI CACCTGC 1 cut(s) 202
PceI AGGCCT 1 cut(s) 638
PctI GAATGC 1 cut(s) 1128
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 3 cut(s) 168, 461, 769
PfoI TCCNGGA 1 cut(s) 719
PkrI GCNGC 6 cut(s) 361, 671, 706, 938, 949, 1236
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 573
PshAI GACNNNNGTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 417
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 574, 912
PspPI GGNCC 2 cut(s) 176, 573
PspPPI RGGWCCY 1 cut(s) 573
PstI CTGCAG 2 cut(s) 199, 735
PstNI CAGNNNCTG 2 cut(s) 221, 338
PsuI RGATCY 2 cut(s) 217, 714
RsaI GTAC 2 cut(s) 245, 526
RsaNI GTAC 2 cut(s) 244, 525
RseI CAYNNNNRTG 2 cut(s) 1158, 1263
SaqAI TTAA 8 cut(s) 278, 660, 882, 906, 927, 977, 1064, 1115
SatI GCNGC 6 cut(s) 360, 670, 705, 937, 948, 1235
Sau3AI GATC 5 cut(s) 217, 405, 652, 714, 1195
Sau96I GGNCC 2 cut(s) 176, 573
SchI GAGTC 1 cut(s) 10
ScrFI CCNGG 2 cut(s) 419, 721
SduI GDGCHC 1 cut(s) 1228
SfaNI GCATC 5 cut(s) 79, 344, 420, 691, 1199
SfcI CTRYAG 6 cut(s) 180, 195, 427, 731, 967, 1044
SfuI TTCGAA 1 cut(s) 859
SinI GGWCC 2 cut(s) 176, 573
SmiMI CAYNNNNRTG 2 cut(s) 1158, 1263
SmlI CTYRAG 1 cut(s) 1063
SmoI CTYRAG 1 cut(s) 1063
SphI GCATGC 1 cut(s) 936
Sse9I AATT 5 cut(s) 298, 678, 751, 924, 1165
SseBI AGGCCT 1 cut(s) 638
SsiI CCGC 6 cut(s) 236, 306, 409, 937, 1074, 1146
SspI AATATT 2 cut(s) 864, 1090
SspMI CTAG 2 cut(s) 162, 1282
StuI AGGCCT 1 cut(s) 638
StyD4I CCNGG 2 cut(s) 417, 719
TaaI ACNGT 7 cut(s) 8, 109, 393, 524, 613, 794, 1048
TaqI TCGA 3 cut(s) 314, 777, 859
TasI AATT 5 cut(s) 298, 678, 751, 924, 1165
TatI WGTACW 1 cut(s) 524
TauI GCSGC 1 cut(s) 939
TfiI GAWTC 3 cut(s) 168, 461, 769
Tru1I TTAA 8 cut(s) 278, 660, 882, 906, 927, 977, 1064, 1115
Tru9I TTAA 8 cut(s) 278, 660, 882, 906, 927, 977, 1064, 1115
TscAI CASTG 3 cut(s) 588, 1053, 1233
TseFI GTSAC 2 cut(s) 94, 788
TseI GCWGC 5 cut(s) 359, 669, 704, 947, 1234
Tsp45I GTSAC 2 cut(s) 94, 788
TspDTI ATGAA 1 cut(s) 1146
TspRI CASTG 3 cut(s) 588, 1053, 1233
Vha464I CTTAAG 1 cut(s) 1063
VpaK11BI GGWCC 2 cut(s) 176, 573
XapI RAATTY 2 cut(s) 751, 1165
XceI RCATGY 1 cut(s) 936
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 2 cut(s) 162, 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.