MD03G1231600.v1.1

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004657
Physical Location & Seq
Reverse (-)
31709118 .. 31712521
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1231600.v1.1.491

Sequence Viewer

Length: 396 bp
ATGGGAAGGATATTTGTGGTAGAGCTGGAGGGAAGGTGCTACAAATGCAAGTTCTGCAAAACTCACTTGGCCCTCTTCTCCGACTGTGTCTCTCGGGCTTTTCATTGCCGCCGAGGAAAAGCATACCTCTTCAATAATGCGGTGAATACCACTACTGGAACATTAGAGGAGAGGATGATGCTCTCGGGTTTGCATACTGTTGCAGATATATTTTGCTGCTCTTGTGGCCAAATTGTTGGCTGGAAATATGAATCTGCACACGAGAACAGCCAGAAGTATAAAGAGGGAAAGTTCGTCCTTGAAAGAGGAAGGATCGTAGACGAAGTAGATTTTTCCGCAGAGTACTTTGTTGATACTCGTCCTAGCATGAGTGGTAGTGACGCAGACGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

132

Amino Acids

14.83

Weight (kDa)

6.41

Isoelectric Point (pI)

15.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 13 - 105 1.5e-11 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 318
AciI CCGC 3 cut(s) 109, 140, 336
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 1 cut(s) 226
AfaI GTAC 1 cut(s) 344
AgsI TTSAA 2 cut(s) 133, 302
AluBI AGCT 1 cut(s) 25
AluI AGCT 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 1 cut(s) 320
Ama87I CYCGRG 2 cut(s) 93, 184
AoxI GGCC 2 cut(s) 69, 226
ApeKI GCWGC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 154
AvaI CYCGRG 2 cut(s) 93, 184
BalI TGGCCA 1 cut(s) 228
BauI CACGAG 1 cut(s) 260
BbvI GCAGC 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 1 cut(s) 363
BisI GCNGC 2 cut(s) 109, 217
BlsI GCNGC 2 cut(s) 110, 218
BmcAI AGTACT 1 cut(s) 344
BmeT110I CYCGRG 2 cut(s) 93, 184
BmgT120I GGNCC 1 cut(s) 70
BmsI GCATC 2 cut(s) 168, 379
BpmI CTGGAG 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 180
BseMI GCAATG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 203
BsgI GTGCAG 1 cut(s) 240
BshFI GGCC 2 cut(s) 71, 228
BsiHKCI CYCGRG 2 cut(s) 93, 184
BsmAI GTCTC 1 cut(s) 94
BsnI GGCC 2 cut(s) 71, 228
BsoBI CYCGRG 2 cut(s) 93, 184
Bsp143I GATC 1 cut(s) 312
BspACI CCGC 3 cut(s) 109, 140, 336
BspANI GGCC 2 cut(s) 71, 228
BspPI GGATC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 103
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 1 cut(s) 312
BssSI CACGAG 1 cut(s) 260
Bst2BI CACGAG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 86, 199
Bst6I CTCTTC 2 cut(s) 80, 134
BstAPI GCANNNNNTGC 2 cut(s) 54, 389
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 1 cut(s) 315
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 1 cut(s) 312
BstMWI GCNNNNNNNGC 4 cut(s) 45, 54, 225, 389
BstV1I GCAGC 1 cut(s) 203
BstXI CCANNNNNNTGG 1 cut(s) 236
BsuRI GGCC 2 cut(s) 71, 228
BtsCI GGATG 1 cut(s) 180
Cfr13I GGNCC 1 cut(s) 70
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 343
CviAII CATG 1 cut(s) 367
CviJI RGCY 6 cut(s) 25, 71, 98, 228, 240, 270
CviKI_1 RGCY 6 cut(s) 25, 71, 98, 228, 240, 270
CviQI GTAC 1 cut(s) 343
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
EaeI YGGCCR 1 cut(s) 226
Eam1104I CTCTTC 2 cut(s) 80, 134
EarI CTCTTC 2 cut(s) 80, 134
Eco88I CYCGRG 2 cut(s) 93, 184
EcoT22I ATGCAT 1 cut(s) 394
FaeI CATG 1 cut(s) 370
FaiI YATR 7 cut(s) 124, 195, 209, 249, 279, 368, 394
FatI CATG 1 cut(s) 366
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 2 cut(s) 109, 217
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 2 cut(s) 109, 217
FspBI CTAG 1 cut(s) 363
GluI GCNGC 2 cut(s) 109, 217
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 2 cut(s) 71, 228
HgaI GACGC 1 cut(s) 389
Hin1II CATG 1 cut(s) 370
HinfI GANTC 1 cut(s) 251
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 82
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 2 cut(s) 27, 303
HpyCH4III ACNGT 2 cut(s) 86, 199
HpyCH4V TGCA 6 cut(s) 48, 57, 193, 203, 257, 392
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 54, 225, 389
Hsp92II CATG 1 cut(s) 370
Kzo9I GATC 1 cut(s) 312
LpnPI CCDG 4 cut(s) 11, 141, 226, 284
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 2 cut(s) 168, 379
MaeI CTAG 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 377
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 2 cut(s) 67, 121
MlsI TGGCCA 1 cut(s) 228
MluCI AATT 1 cut(s) 231
MluNI TGGCCA 1 cut(s) 228
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 8 cut(s) 22, 83, 107, 137, 160, 165, 277, 299
Mox20I TGGCCA 1 cut(s) 228
Mph1103I ATGCAT 1 cut(s) 394
MscI TGGCCA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 228
MwoI GCNNNNNNNGC 4 cut(s) 45, 54, 225, 389
NdeII GATC 1 cut(s) 312
NlaIII CATG 1 cut(s) 370
NmeAIII GCCGAG 1 cut(s) 137
NmuCI GTSAC 1 cut(s) 377
NsiI ATGCAT 1 cut(s) 394
PfeI GAWTC 1 cut(s) 251
PflFI GACNNNGTC 1 cut(s) 86
PkrI GCNGC 2 cut(s) 110, 218
PspPI GGNCC 1 cut(s) 70
PsyI GACNNNGTC 1 cut(s) 86
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
SatI GCNGC 2 cut(s) 109, 217
Sau3AI GATC 1 cut(s) 312
Sau96I GGNCC 1 cut(s) 70
ScaI AGTACT 1 cut(s) 344
SetI ASST 3 cut(s) 27, 38, 129
SfaNI GCATC 2 cut(s) 168, 379
Sse9I AATT 1 cut(s) 231
SsiI CCGC 3 cut(s) 109, 140, 336
SspMI CTAG 1 cut(s) 363
TaaI ACNGT 2 cut(s) 86, 199
TasI AATT 1 cut(s) 231
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 1 cut(s) 251
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 1 cut(s) 216
Tsp45I GTSAC 1 cut(s) 377
TspDTI ATGAA 2 cut(s) 92, 264
Tth111I GACNNNGTC 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 318
XspI CTAG 1 cut(s) 363
ZrmI AGTACT 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.