MD05G1311500.v1.1

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004657
Physical Location & Seq
Forward (+)
44149607 .. 44154358
4752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1311500.v1.1.491

Sequence Viewer

Length: 384 bp
ATGGGGAGAATTTTTCTGATTGAATTGGACGGCAGAACTTACAGATGCAGATTCTGTGACAGCGCTCTTGCCCTTGCCGACGATGTCCTCTCTCGGTCTTTCAACAGCCATCAAGGAAGGGCATATCTGTTCAGCAATGTGGTGAACATAACGCTCGGTCCTGAGGAAGATAGGCTAATGTACTCTGGTTTGCATACTGTGGAAGATATTTTTTGTTGCTGCTGCGGACAGATTCTTGGCTGGAAATATGTAATTGCACACGAAAAAAACCAAAAATACAAGGAAGGAAAGTTTGTGCTCGAAAGATGGAGGATAGTGGAGGATGTAGCTGAGGAATTCAACTTGGACGCTCGTCTTGGTTCAAGTGATGGAGAAAATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

128

Amino Acids

14.58

Weight (kDa)

5.17

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 14 - 105 1.1e-09 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 225
AcsI RAATTY 2 cut(s) 9, 335
AfaI GTAC 1 cut(s) 182
AfeI AGCGCT 1 cut(s) 64
AgsI TTSAA 4 cut(s) 23, 103, 340, 363
AluBI AGCT 1 cut(s) 329
AluI AGCT 1 cut(s) 329
Alw21I GWGCWC 1 cut(s) 300
AlwNI CAGNNNCTG 1 cut(s) 54
Aor51HI AGCGCT 1 cut(s) 64
ApeKI GCWGC 2 cut(s) 219, 222
ApoI RAATTY 2 cut(s) 9, 335
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 158
AxyI CCTNAGG 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 206, 209
BccI CCATC 3 cut(s) 117, 300, 362
BceAI ACGGC 1 cut(s) 46
BfaI CTAG 1 cut(s) 382
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 2 cut(s) 220, 223
BlsI GCNGC 2 cut(s) 221, 224
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmsI GCATC 1 cut(s) 35
BoxI GACNNNNGTC 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 330
Bse21I CCTNAGG 1 cut(s) 162
Bse3DI GCAATG 1 cut(s) 142
BseGI GGATG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 153, 321
BseXI GCAGC 2 cut(s) 206, 209
BsiHKAI GWGCWC 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 300
BspACI CCGC 1 cut(s) 225
BspCNI CTCAG 2 cut(s) 154, 322
BsrDI GCAATG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 199
BstDEI CTNAG 2 cut(s) 162, 330
BstF5I GGATG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 1 cut(s) 65
BstPAI GACNNNNGTC 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 206, 209
Bsu36I CCTNAGG 1 cut(s) 162
BtsCI GGATG 1 cut(s) 328
CaiI CAGNNNCTG 1 cut(s) 54
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 181
CviJI RGCY 4 cut(s) 108, 175, 240, 329
CviKI_1 RGCY 4 cut(s) 108, 175, 240, 329
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 2 cut(s) 162, 330
Eco47I GGWCC 1 cut(s) 158
Eco47III AGCGCT 1 cut(s) 64
Eco81I CCTNAGG 1 cut(s) 162
EcoRI GAATTC 1 cut(s) 335
FaiI YATR 4 cut(s) 124, 149, 195, 249
Fnu4HI GCNGC 2 cut(s) 220, 223
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 2 cut(s) 220, 223
FspBI CTAG 1 cut(s) 382
GlaI GCGC 1 cut(s) 64
GluI GCNGC 2 cut(s) 220, 223
HaeII RGCGCY 1 cut(s) 66
HgaI GACGC 1 cut(s) 356
HhaI GCGC 1 cut(s) 65
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HinfI GANTC 2 cut(s) 51, 232
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 111, 278
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4V TGCA 3 cut(s) 48, 193, 257
HpyF3I CTNAG 2 cut(s) 162, 330
HspAI GCGC 1 cut(s) 63
LpnPI CCDG 3 cut(s) 171, 174, 226
Lsp1109I GCAGC 2 cut(s) 206, 209
LweI GCATC 1 cut(s) 35
MaeI CTAG 1 cut(s) 382
MaeIII GTNAC 1 cut(s) 56
MboII GAAGA 2 cut(s) 179, 215
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 4 cut(s) 9, 23, 252, 335
MnlI CCTC 5 cut(s) 98, 157, 303, 313, 325
NmuCI GTSAC 1 cut(s) 56
PfeI GAWTC 2 cut(s) 51, 232
PflFI GACNNNGTC 1 cut(s) 83
PkrI GCNGC 2 cut(s) 221, 224
PshAI GACNNNNGTC 1 cut(s) 351
PspPI GGNCC 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 54
PsyI GACNNNGTC 1 cut(s) 83
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SatI GCNGC 2 cut(s) 220, 223
Sau96I GGNCC 1 cut(s) 158
SduI GDGCHC 1 cut(s) 300
SetI ASST 1 cut(s) 331
SfaNI GCATC 1 cut(s) 35
SinI GGWCC 1 cut(s) 158
Sse9I AATT 4 cut(s) 9, 23, 252, 335
SsiI CCGC 1 cut(s) 225
SspMI CTAG 1 cut(s) 382
TaaI ACNGT 1 cut(s) 199
TaqI TCGA 1 cut(s) 300
TaqII GACCGA 2 cut(s) 84, 146
TasI AATT 4 cut(s) 9, 23, 252, 335
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 2 cut(s) 51, 232
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 2 cut(s) 219, 222
Tsp45I GTSAC 1 cut(s) 56
Tth111I GACNNNGTC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 2 cut(s) 9, 335
XspI CTAG 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.