MD03G1235300.v1.1

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013014
Physical Location & Seq
Forward (+)
32143155 .. 32146352
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1235300.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGCCTCGACCTCTCGCACTCATGGCGTCACGGGCCGGATCCGAACCGCCACGGCCAATCTCCACGAGGACAACCAGTCTCTTATCTCCGATATTGGGAAAGCTTTCAGTTTGATGAAGGAAATTGCAGTCGATTTCGAGAGTGCTAAGCAATTTGAGAAGGTGAAGGAGCTCGAAAATGCCGTTGTTGAATTGGTGGCTACTTATGAAGACTGTTCACATTTTTCGTCAGCAATTGAATCTGTTGGACATAAATACCAACCTGGGCCTGAGCTTACTGATTTCGATAAGTTGTTCAAGAATGAGGTTGCACAGCTGAAGGCAAACTCATCTTCAGTTCCCCAAAACCATCCTCTGATACGTCAATTCCGGGAAGTTGTCTGGAAAGTCCATCACGCAGGAGAACCTATGCCAGGTGAAGAGCAGGAGGACATTGTAATGACGAGCAACCAAAGCACTATTTTGAATGTCACTTGTCCATTAAGTGGAAAGCCTGTCACTGAACTGGAGCATCCAGTTCGCAGTGTCGTATGCAAGCATGTTTACGACAAAGGACATATCATGCATTACTTACGGGAAAACAACACACGATGCCCTGTTGCAGCTTGCCCTGCGAAATTGAAGTCAGATAAAGTGAAGGATGATCCACTGTTAGCCGCTGATATTGATGAATTGCGTAAAACGCAGAATCAGAATGTCATGACTGATGTGATGGACGTCACTGAGTTTGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

246

Amino Acids

27.44

Weight (kDa)

5.48

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Nse PF11789 143 - 203 2.7e-13 Zinc-finger of the MIZ type in Nse subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 720
AccB7I CCANNNNNTGG 1 cut(s) 485
AciI CCGC 2 cut(s) 48, 657
AclWI GGATC 3 cut(s) 34, 47, 638
AcoI YGGCCR 1 cut(s) 54
AcuI CTGAAG 2 cut(s) 318, 338
AcyI GRCGYC 2 cut(s) 27, 717
AfiI CCNNNNNNNGG 3 cut(s) 96, 413, 485
AgsI TTSAA 6 cut(s) 191, 239, 298, 466, 622, 731
AhdI GACNNNNNGTC 1 cut(s) 76
AjnI CCWGG 2 cut(s) 262, 412
AluBI AGCT 5 cut(s) 104, 172, 274, 316, 605
AluI AGCT 5 cut(s) 104, 172, 274, 316, 605
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 3 cut(s) 34, 47, 638
AoxI GGCC 4 cut(s) 3, 34, 54, 266
ApeKI GCWGC 1 cut(s) 602
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 1 cut(s) 104
AspS9I GGNCC 2 cut(s) 34, 266
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 2 cut(s) 175, 428
BamHI GGATCC 1 cut(s) 39
BanII GRGCYC 1 cut(s) 174
BauI CACGAG 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 614
BccI CCATC 3 cut(s) 357, 399, 706
BceAI ACGGC 2 cut(s) 69, 167
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 2 cut(s) 264, 414
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 84
BisI GCNGC 2 cut(s) 603, 657
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 2 cut(s) 604, 658
Bme1390I CCNGG 3 cut(s) 264, 371, 414
BmeRI GACNNNNNGTC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 34, 266
BmiI GGNNCC 1 cut(s) 41
BmrFI CCNGG 3 cut(s) 264, 371, 414
BmsI GCATC 2 cut(s) 520, 581
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 371
BsaHI GRCGYC 2 cut(s) 27, 717
BsaJI CCNNGG 2 cut(s) 51, 263
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 413, 485
Bse1I ACTGG 3 cut(s) 76, 510, 515
BseBI CCWGG 2 cut(s) 264, 414
BseDI CCNNGG 2 cut(s) 51, 263
BseGI GGATG 3 cut(s) 349, 511, 646
BseLI CCNNNNNNNGG 3 cut(s) 96, 413, 485
BseMII CTCAG 2 cut(s) 261, 714
BseNI ACTGG 3 cut(s) 76, 510, 515
BseXI GCAGC 1 cut(s) 614
BshFI GGCC 4 cut(s) 5, 36, 56, 268
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 2 cut(s) 37, 370
BslI CCNNNNNNNGG 3 cut(s) 96, 413, 485
BsmAI GTCTC 1 cut(s) 84
BsnI GGCC 4 cut(s) 5, 36, 56, 268
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 39, 643
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 2 cut(s) 48, 657
BspANI GGCC 4 cut(s) 5, 36, 56, 268
BspCNI CTCAG 2 cut(s) 262, 715
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 1 cut(s) 41
BspPI GGATC 3 cut(s) 34, 47, 638
BspQI GCTCTTC 1 cut(s) 414
BsrI ACTGG 3 cut(s) 76, 510, 515
BssECI CCNNGG 2 cut(s) 51, 263
BssMI GATC 2 cut(s) 39, 643
BssNI GRCGYC 2 cut(s) 27, 717
BssSI CACGAG 1 cut(s) 65
Bst2BI CACGAG 1 cut(s) 65
Bst2UI CCWGG 2 cut(s) 264, 414
Bst4CI ACNGT 2 cut(s) 215, 651
Bst6I CTCTTC 2 cut(s) 414, 726
BstACI GRCGYC 2 cut(s) 27, 717
BstC8I GCNNGC 2 cut(s) 536, 607
BstDEI CTNAG 3 cut(s) 147, 270, 723
BstDSI CCRYGG 1 cut(s) 51
BstENI CCTNNNNNAGG 1 cut(s) 411
BstF5I GGATG 3 cut(s) 349, 511, 646
BstKTI GATC 2 cut(s) 42, 646
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 2 cut(s) 39, 643
BstMWI GCNNNNNNNGC 5 cut(s) 24, 33, 453, 611, 682
BstNI CCWGG 2 cut(s) 264, 414
BstNSI RCATGY 1 cut(s) 542
BstSCI CCNGG 3 cut(s) 262, 369, 412
BstV1I GCAGC 1 cut(s) 614
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 39
BstYI RGATCY 1 cut(s) 39
BsuRI GGCC 4 cut(s) 5, 36, 56, 268
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 3 cut(s) 349, 511, 646
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 4 cut(s) 498, 529, 647, 720
Cac8I GCNNGC 2 cut(s) 536, 607
CciI TCATGA 1 cut(s) 699
Cfr13I GGNCC 2 cut(s) 34, 266
CseI GACGC 1 cut(s) 16
CviAII CATG 4 cut(s) 23, 539, 562, 700
DdeI CTNAG 3 cut(s) 147, 270, 723
DpnI GATC 2 cut(s) 41, 645
DpnII GATC 2 cut(s) 39, 643
DriI GACNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 414, 726
Eam1105I GACNNNNNGTC 1 cut(s) 76
EarI CTCTTC 2 cut(s) 414, 726
Ecl136II GAGCTC 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 318, 338
EcoICRI GAGCTC 1 cut(s) 172
EcoNI CCTNNNNNAGG 1 cut(s) 411
EcoRII CCWGG 2 cut(s) 262, 412
EcoT22I ATGCAT 1 cut(s) 567
EcoT38I GRGCYC 1 cut(s) 174
FaeI CATG 4 cut(s) 26, 542, 565, 703
FaiI YATR 9 cut(s) 24, 207, 252, 410, 532, 540, 558, 563, 701
FatI CATG 4 cut(s) 22, 538, 561, 699
Fnu4HI GCNGC 2 cut(s) 603, 657
FokI GGATG 3 cut(s) 336, 498, 653
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 603, 657
GluI GCNGC 2 cut(s) 603, 657
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 4 cut(s) 5, 36, 56, 268
HapII CCGG 2 cut(s) 37, 370
HgaI GACGC 1 cut(s) 16
Hin1I GRCGYC 2 cut(s) 27, 717
Hin1II CATG 4 cut(s) 26, 542, 565, 703
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 2 cut(s) 239, 688
HpaII CCGG 2 cut(s) 37, 370
HphI GGTGA 2 cut(s) 175, 428
Hpy166II GTNNAC 2 cut(s) 218, 544
Hpy188I TCNGA 5 cut(s) 44, 91, 357, 628, 693
Hpy188III TCNNGA 4 cut(s) 139, 298, 382, 700
Hpy8I GTNNAC 2 cut(s) 218, 544
HpyAV CCTTC 5 cut(s) 112, 154, 160, 313, 631
HpyCH4III ACNGT 2 cut(s) 215, 651
HpyCH4IV ACGT 2 cut(s) 361, 717
HpyCH4V TGCA 5 cut(s) 128, 311, 534, 565, 602
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 33, 453, 611, 682
HpyF3I CTNAG 3 cut(s) 147, 270, 723
HpySE526I ACGT 2 cut(s) 361, 717
Hsp92I GRCGYC 2 cut(s) 27, 717
Hsp92II CATG 4 cut(s) 26, 542, 565, 703
Kzo9I GATC 2 cut(s) 39, 643
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 2 cut(s) 169, 508
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 2 cut(s) 520, 581
MaeII ACGT 2 cut(s) 361, 717
MaeIII GTNAC 4 cut(s) 28, 469, 496, 718
MalI GATC 2 cut(s) 41, 645
MboI GATC 2 cut(s) 39, 643
MboII GAAGA 3 cut(s) 221, 324, 431
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 7 cut(s) 123, 152, 191, 234, 365, 617, 671
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 6 cut(s) 16, 22, 61, 298, 363, 421
Mph1103I ATGCAT 1 cut(s) 567
MroXI GAANNNNTTC 1 cut(s) 104
MseI TTAA 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 437
MspA1I CMGCKG 2 cut(s) 316, 659
MspI CCGG 2 cut(s) 37, 370
MspR9I CCNGG 3 cut(s) 264, 371, 414
MunI CAATTG 1 cut(s) 234
MvaI CCWGG 2 cut(s) 264, 414
MwoI GCNNNNNNNGC 5 cut(s) 24, 33, 453, 611, 682
NciI CCSGG 1 cut(s) 371
NdeII GATC 2 cut(s) 39, 643
NlaIII CATG 4 cut(s) 26, 542, 565, 703
NlaIV GGNNCC 1 cut(s) 41
NmuCI GTSAC 4 cut(s) 28, 469, 496, 718
NsiI ATGCAT 1 cut(s) 567
NspI RCATGY 1 cut(s) 542
PagI TCATGA 1 cut(s) 699
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 2 cut(s) 239, 688
PflMI CCANNNNNTGG 1 cut(s) 485
PfoI TCCNGGA 1 cut(s) 369
PkrI GCNGC 2 cut(s) 604, 658
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 262, 412
PspGI CCWGG 2 cut(s) 262, 412
PspN4I GGNNCC 1 cut(s) 41
PspPI GGNCC 2 cut(s) 34, 266
PsuI RGATCY 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 437
SacI GAGCTC 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 2 cut(s) 603, 657
Sau3AI GATC 2 cut(s) 39, 643
Sau96I GGNCC 2 cut(s) 34, 266
ScrFI CCNGG 3 cut(s) 264, 371, 414
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 2 cut(s) 520, 581
SmiMI CAYNNNNRTG 1 cut(s) 437
Sse9I AATT 7 cut(s) 123, 152, 191, 234, 365, 617, 671
SsiI CCGC 2 cut(s) 48, 657
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 262, 369, 412
TaaI ACNGT 2 cut(s) 215, 651
TaiI ACGT 2 cut(s) 364, 720
TaqI TCGA 5 cut(s) 8, 132, 138, 174, 285
TasI AATT 7 cut(s) 123, 152, 191, 234, 365, 617, 671
TauI GCSGC 1 cut(s) 659
TfiI GAWTC 2 cut(s) 239, 688
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 4 cut(s) 505, 529, 654, 727
TseFI GTSAC 4 cut(s) 28, 469, 496, 718
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 4 cut(s) 28, 469, 496, 718
TspDTI ATGAA 3 cut(s) 131, 222, 684
TspRI CASTG 4 cut(s) 505, 529, 654, 727
Van91I CCANNNNNTGG 1 cut(s) 485
XagI CCTNNNNNAGG 1 cut(s) 411
XceI RCATGY 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 104
ZraI GACGTC 1 cut(s) 718
Zsp2I ATGCAT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.