Rroxscaffold_1G00048090

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013014
Physical Location & Seq
Forward (+)
68114029 .. 68118449
4421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00048090.1

Sequence Viewer

Length: 783 bp
ATGGCCTCGACCTCCTCTCGGACTCATGGCATCGCCGGTCGGATCCGAACCGCCGCAACCAATCTTTACTCCGACAACCAGTCCCTGCTCGGCGATATTCGAAAATCTTTCAATGTGTTGAAGGAAGTTGCTGTTGATTTGGAGAGAGCCAATCAGTTTGACAAGGTGAAGGAGATTGAAAATGCTGCTGTTGAATTGGTGGCTACTTGTGAACACTGTATGCATCTCTCATCAGCAATTCAATATGTTGGAGATAACTACGAACCTCCTCCAGAGCTTACTGATTTTGAGAAGTTGCTCAAAAATGAGGTTGCAAAACAAAAGGCTAAATCATCTTCAACTACAGTAAACCAGTCTATGATACGTCAATTTCAGGAAGCTGTCTGGAATGTTCATCATGCAGGACAGCCTATGCCAGGTGACGACCAGGAGGACATCGTAATGACTAGTACTGATGGCAATCTTCGGAATACCACTTGCCCAGTAAGTGGAAAGGCTATCACGGATTTGGAAGATCCAGTCTGCAGTGTTGACTGCAAGCATATATATCAAACAAAGGAGATTATGCATTACTTGCAGTTAAAGGGGGGACGTGGACCATGCCCAATTGCAGGTTGTCCTAGGATGTTGCAGGCGGACAAATTGGTTCGTGATCCATTGCTACTAGTTGAAATTCAAGAATTGCGTGATATGAGGAAGCACCAGACCGCTGATACTAACGAGCTCATATCCGCCCAAGTTGACGCATTGTCTTTCTTAATTTGCTTGCAAGCAACCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

260

Amino Acids

28.83

Weight (kDa)

5.5

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Nse PF11789 145 - 206 2.3e-08 Zinc-finger of the MIZ type in Nse subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 602
AccB7I CCANNNNNTGG 1 cut(s) 488
AciI CCGC 5 cut(s) 51, 54, 635, 708, 732
AclWI GGATC 4 cut(s) 37, 50, 509, 647
AcsI RAATTY 1 cut(s) 672
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 4 cut(s) 18, 416, 488, 611
AgsI TTSAA 8 cut(s) 112, 121, 179, 194, 242, 339, 671, 677
AhdI GACNNNNNGTC 2 cut(s) 79, 748
AhlI ACTAGT 2 cut(s) 446, 664
AjiI CACGTC 1 cut(s) 593
AjnI CCWGG 2 cut(s) 415, 426
AluBI AGCT 3 cut(s) 277, 380, 724
AluI AGCT 3 cut(s) 277, 380, 724
Alw21I GWGCWC 1 cut(s) 726
AlwI GGATC 4 cut(s) 37, 50, 509, 647
AlwNI CAGNNNCTG 1 cut(s) 85
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 1 cut(s) 672
AspA2I CCTAGG 1 cut(s) 620
AspS9I GGNCC 1 cut(s) 596
AsuHPI GGTGA 2 cut(s) 178, 431
AsuII TTCGAA 1 cut(s) 100
AvaII GGWCC 1 cut(s) 596
AvrII CCTAGG 1 cut(s) 620
BamHI GGATCC 1 cut(s) 42
BanII GRGCYC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 1 cut(s) 172
BccI CCATC 1 cut(s) 449
BciT130I CCWGG 2 cut(s) 417, 428
BcuI ACTAGT 2 cut(s) 446, 664
BfaI CTAG 3 cut(s) 447, 621, 665
BfmI CTRYAG 2 cut(s) 342, 523
BfuAI ACCTGC 1 cut(s) 602
BisI GCNGC 2 cut(s) 54, 186
BlnI CCTAGG 1 cut(s) 620
BlsI GCNGC 2 cut(s) 55, 187
BmcAI AGTACT 1 cut(s) 451
Bme1390I CCNGG 2 cut(s) 417, 428
Bme18I GGWCC 1 cut(s) 596
BmeRI GACNNNNNGTC 2 cut(s) 79, 748
BmgBI CACGTC 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 596
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 2 cut(s) 417, 428
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 2 cut(s) 39, 232
BmuI ACTGGG 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 255
Bpu14I TTCGAA 1 cut(s) 100
BsaBI GATNNNNATC 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 620
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 416, 488, 611
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 4 cut(s) 79, 352, 482, 518
Bse3DI GCAATG 1 cut(s) 656
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 2 cut(s) 417, 428
BseDI CCNNGG 1 cut(s) 620
BseGI GGATG 1 cut(s) 630
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 4 cut(s) 18, 416, 488, 611
BseMI GCAATG 1 cut(s) 656
BseNI ACTGG 4 cut(s) 79, 352, 482, 518
BseRI GAGGAG 2 cut(s) 4, 258
BseXI GCAGC 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 726
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 2 cut(s) 67, 603
BslI CCNNNNNNNGG 4 cut(s) 18, 416, 488, 611
BsmFI GGGAC 2 cut(s) 67, 603
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 726
Bsp143I GATC 3 cut(s) 42, 514, 652
BspACI CCGC 5 cut(s) 51, 54, 635, 708, 732
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 1 cut(s) 44
BspMAI CTGCAG 1 cut(s) 527
BspMI ACCTGC 1 cut(s) 602
BspPI GGATC 4 cut(s) 37, 50, 509, 647
BspT104I TTCGAA 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 656
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 4 cut(s) 79, 352, 482, 518
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 620
BssMI GATC 3 cut(s) 42, 514, 652
BssT1I CCWWGG 1 cut(s) 620
Bst2UI CCWGG 2 cut(s) 417, 428
Bst4CI ACNGT 2 cut(s) 218, 346
BstAPI GCANNNNNTGC 1 cut(s) 574
BstBI TTCGAA 1 cut(s) 100
BstC8I GCNNGC 4 cut(s) 539, 633, 767, 771
BstENI CCTNNNNNAGG 1 cut(s) 414
BstF5I GGATG 1 cut(s) 630
BstKTI GATC 3 cut(s) 45, 517, 655
BstMBI GATC 3 cut(s) 42, 514, 652
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 1 cut(s) 574
BstNI CCWGG 2 cut(s) 417, 428
BstSCI CCNGG 2 cut(s) 415, 426
BstSFI CTRYAG 2 cut(s) 342, 523
BstV1I GCAGC 1 cut(s) 172
BstX2I RGATCY 2 cut(s) 42, 514
BstYI RGATCY 2 cut(s) 42, 514
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 16
BtrI CACGTC 1 cut(s) 593
BtsCI GGATG 1 cut(s) 630
BtsI GCAGTG 1 cut(s) 532
BtsIMutI CAGTG 2 cut(s) 214, 532
BveI ACCTGC 1 cut(s) 602
Cac8I GCNNGC 4 cut(s) 539, 633, 767, 771
CaiI CAGNNNCTG 1 cut(s) 85
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 596
CseI GACGC 1 cut(s) 752
Csp6I GTAC 1 cut(s) 450
CviAII CATG 3 cut(s) 26, 398, 600
CviJI RGCY 9 cut(s) 5, 149, 203, 277, 326, 380, 409, 497, 724
CviKI_1 RGCY 9 cut(s) 5, 149, 203, 277, 326, 380, 409, 497, 724
CviQI GTAC 1 cut(s) 450
DpnI GATC 3 cut(s) 44, 516, 654
DpnII GATC 3 cut(s) 42, 514, 652
DriI GACNNNNNGTC 2 cut(s) 79, 748
Eam1105I GACNNNNNGTC 2 cut(s) 79, 748
EciI GGCGGA 2 cut(s) 650, 721
Ecl136II GAGCTC 1 cut(s) 724
Eco130I CCWWGG 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 726
Eco47I GGWCC 1 cut(s) 596
Eco53kI GAGCTC 1 cut(s) 724
EcoICRI GAGCTC 1 cut(s) 724
EcoNI CCTNNNNNAGG 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 415, 426
EcoT14I CCWWGG 1 cut(s) 620
EcoT22I ATGCAT 2 cut(s) 225, 570
EcoT38I GRGCYC 1 cut(s) 726
ErhI CCWWGG 1 cut(s) 620
FaeI CATG 3 cut(s) 29, 401, 603
FaqI GGGAC 2 cut(s) 67, 603
FatI CATG 3 cut(s) 25, 397, 599
Fnu4HI GCNGC 2 cut(s) 54, 186
FokI GGATG 1 cut(s) 637
FriOI GRGCYC 1 cut(s) 726
Fsp4HI GCNGC 2 cut(s) 54, 186
FspBI CTAG 3 cut(s) 447, 621, 665
GluI GCNGC 2 cut(s) 54, 186
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 752
Hin1II CATG 3 cut(s) 29, 401, 603
HincII GTYRAC 2 cut(s) 532, 742
HindII GTYRAC 2 cut(s) 532, 742
HinfI GANTC 1 cut(s) 22
HpaII CCGG 1 cut(s) 36
HphI GGTGA 2 cut(s) 178, 431
Hpy166II GTNNAC 5 cut(s) 212, 349, 532, 596, 742
Hpy188I TCNGA 5 cut(s) 21, 42, 47, 73, 468
Hpy188III TCNNGA 5 cut(s) 272, 374, 385, 650, 677
Hpy8I GTNNAC 5 cut(s) 212, 349, 532, 596, 742
HpyAV CCTTC 2 cut(s) 115, 163
HpyCH4III ACNGT 2 cut(s) 218, 346
HpyCH4IV ACGT 2 cut(s) 364, 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 574
HpySE526I ACGT 2 cut(s) 364, 592
Hsp92II CATG 3 cut(s) 29, 401, 603
Kzo9I GATC 3 cut(s) 42, 514, 652
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 2 cut(s) 39, 232
MaeI CTAG 3 cut(s) 447, 621, 665
MaeII ACGT 2 cut(s) 364, 592
MaeIII GTNAC 1 cut(s) 419
MalI GATC 3 cut(s) 44, 516, 654
MboI GATC 3 cut(s) 42, 514, 652
MboII GAAGA 3 cut(s) 327, 455, 524
MfeI CAATTG 1 cut(s) 606
MflI RGATCY 2 cut(s) 42, 514
MhlI GDGCHC 1 cut(s) 726
MluCI AATT 8 cut(s) 194, 237, 368, 606, 641, 672, 680, 759
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 3 cut(s) 20, 96, 229
MnlI CCTC 8 cut(s) 16, 22, 25, 276, 279, 301, 424, 687
Mph1103I ATGCAT 2 cut(s) 225, 570
MseI TTAA 3 cut(s) 581, 758, 781
MslI CAYNNNNRTG 1 cut(s) 440
MspA1I CMGCKG 1 cut(s) 710
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 417, 428
MunI CAATTG 1 cut(s) 606
MvaI CCWGG 2 cut(s) 417, 428
MwoI GCNNNNNNNGC 1 cut(s) 574
NdeII GATC 3 cut(s) 42, 514, 652
NlaIII CATG 3 cut(s) 29, 401, 603
NlaIV GGNNCC 1 cut(s) 44
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 419
NsiI ATGCAT 2 cut(s) 225, 570
NspV TTCGAA 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 488
PkrI GCNGC 2 cut(s) 55, 187
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp124BI GAGCTC 1 cut(s) 726
Psp6I CCWGG 2 cut(s) 415, 426
PspGI CCWGG 2 cut(s) 415, 426
PspN4I GGNNCC 1 cut(s) 44
PspPI GGNCC 1 cut(s) 596
PstI CTGCAG 1 cut(s) 527
PstNI CAGNNNCTG 1 cut(s) 85
PsuI RGATCY 2 cut(s) 42, 514
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 440
SacI GAGCTC 1 cut(s) 726
SaqAI TTAA 3 cut(s) 581, 758, 781
SatI GCNGC 2 cut(s) 54, 186
Sau3AI GATC 3 cut(s) 42, 514, 652
Sau96I GGNCC 1 cut(s) 596
ScaI AGTACT 1 cut(s) 451
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 417, 428
SduI GDGCHC 1 cut(s) 726
SfaNI GCATC 2 cut(s) 39, 232
SfcI CTRYAG 2 cut(s) 342, 523
SfuI TTCGAA 1 cut(s) 100
SinI GGWCC 1 cut(s) 596
SmiMI CAYNNNNRTG 1 cut(s) 440
SpeI ACTAGT 2 cut(s) 446, 664
Sse9I AATT 8 cut(s) 194, 237, 368, 606, 641, 672, 680, 759
SsiI CCGC 5 cut(s) 51, 54, 635, 708, 732
SspMI CTAG 3 cut(s) 447, 621, 665
SstI GAGCTC 1 cut(s) 726
StyD4I CCNGG 2 cut(s) 415, 426
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 2 cut(s) 218, 346
TaiI ACGT 2 cut(s) 367, 595
TaqI TCGA 2 cut(s) 8, 100
TasI AATT 8 cut(s) 194, 237, 368, 606, 641, 672, 680, 759
TatI WGTACW 1 cut(s) 449
TauI GCSGC 1 cut(s) 56
Tru1I TTAA 3 cut(s) 581, 758, 781
Tru9I TTAA 3 cut(s) 581, 758, 781
TscAI CASTG 2 cut(s) 221, 532
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 2 cut(s) 221, 532
Van91I CCANNNNNTGG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 414
XapI RAATTY 1 cut(s) 672
XmaJI CCTAGG 1 cut(s) 620
XspI CTAG 3 cut(s) 447, 621, 665
ZrmI AGTACT 1 cut(s) 451
Zsp2I ATGCAT 2 cut(s) 225, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.