pycom03g18180

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0013014
Physical Location & Seq
Forward (+)
19207084 .. 19210139
3056 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g18180.1

Sequence Viewer

Length: 1047 bp
ATGTATATATTGAAACCTCGACCGTTGGATCATGCCAATGAATCTTGTATAAGGCTAAGATCAAAATGGGATATGTTTCGCTTTGGCCTTGTGATATACGACTCGTCGCATTTCAGAAGAGGAATGGACGATGCCGTTTCGTACTTTTTGTTCTTTCTACTCCGCAGTCTCCCTCCAAAATTGCCCATATATCTCTGTAGTCTGTACTCGAGAACTGTTGCCCTCGCTCCCACCAGCGAAGAACCCGCCAATCAGCCAACCGGTTCGCCCGAACCCGGTTGTCGGAAACCCCTACAACCCTCATCCCTCCATGGCCTCGACCTCTCGCACTCATGGCGTCACGGGCCGGATCCGAACCGCCACCGTCAATCTCCACGAGGACAACCAGTCTCTTATCTCCGTAATGGGAAAGCTTTCAGTTTGATGAAGGAAATTGCAGTCGATTTCGAGAGGGCTAAGCAATTTGAGAAGGTGAAGGAGCTTGAAAATGCCGTTGTTGAATTGGTGGCTACTTATGAAGACTGTTCGCATTTTTCGTCAGCAATTGAATCTGTTGGACATAAATACCAACCTGGGCCTGAGCTTACTGATTTCGATAAGTTGTTCAAGAATGAGGTTGCACAGCTGAAGGCAAACTCATCTTCAGTTCCCCAAAACCATCCTTTGATACGTCAATTCCGGGAAGCTGTCTGGAAGGTCCATCACGCAGGAGAACCTATGCCAGGTGAAGAGCAGGAGGACATTGTAATGACAAGCAACCAAAGCAGTATTTTGAATGTCACTTGTCCGTTAAGTGGAAAGCCTGTCACTGAACTGGAGCATCCAGTTCGCAGTGTCGTATGCAAGCATGTTTACGACAAAGGGCATATCATGCATTACTTACGGGAAAACAACACACGATGCCCTGTTGCAGCTTGCCCTGCGAAATTGAAGTCAGATAAAGTGAAGGATGATCCACTGTTAGCCGCTGATATTGATGAATTGCGTAAATCGCAGAATCAGAATGTCATGACTGATGTGATGGACGTCACTGAGTTTGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

349

Amino Acids

39.55

Weight (kDa)

6.57

Isoelectric Point (pI)

54.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Nse PF11789 246 - 306 2.8e-13 Zinc-finger of the MIZ type in Nse subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1029
AciI CCGC 4 cut(s) 163, 246, 358, 966
AclWI GGATC 4 cut(s) 36, 344, 357, 947
AcuI CTGAAG 2 cut(s) 627, 647
AcyI GRCGYC 2 cut(s) 337, 1026
AfaI GTAC 2 cut(s) 143, 206
AfiI CCNNNNNNNGG 4 cut(s) 275, 282, 722, 794
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 8 cut(s) 13, 485, 500, 548, 607, 775, 931, 1040
AhdI GACNNNNNGTC 1 cut(s) 386
AjnI CCWGG 2 cut(s) 571, 721
AluBI AGCT 6 cut(s) 413, 481, 583, 625, 686, 914
AluI AGCT 6 cut(s) 413, 481, 583, 625, 686, 914
Alw26I GTCTC 2 cut(s) 173, 394
AlwI GGATC 4 cut(s) 36, 344, 357, 947
Ama87I CYCGRG 1 cut(s) 208
AoxI GGCC 4 cut(s) 85, 313, 344, 575
ApeKI GCWGC 1 cut(s) 911
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
AsiGI ACCGGT 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 413
AspS9I GGNCC 3 cut(s) 344, 575, 697
AsuC2I CCSGG 2 cut(s) 276, 680
AsuHPI GGTGA 2 cut(s) 484, 737
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 1 cut(s) 697
BamHI GGATCC 1 cut(s) 349
BauI CACGAG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 525
BbvI GCAGC 1 cut(s) 923
BccI CCATC 3 cut(s) 666, 708, 1015
BceAI ACGGC 2 cut(s) 119, 476
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 2 cut(s) 573, 723
BcnI CCSGG 2 cut(s) 276, 680
BcoDI GTCTC 2 cut(s) 173, 394
BfmI CTRYAG 1 cut(s) 196
BisI GCNGC 2 cut(s) 912, 966
BlpI GCTNAGC 1 cut(s) 456
BlsI GCNGC 2 cut(s) 913, 967
Bme1390I CCNGG 4 cut(s) 276, 573, 680, 723
Bme18I GGWCC 1 cut(s) 697
BmeRI GACNNNNNGTC 1 cut(s) 386
BmeT110I CYCGRG 1 cut(s) 208
BmgT120I GGNCC 3 cut(s) 344, 575, 697
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 4 cut(s) 276, 573, 680, 723
BmsI GCATC 3 cut(s) 121, 829, 890
BpiI GAAGAC 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 836
Bpu10I CCTNAGC 1 cut(s) 579
Bpu1102I GCTNAGC 1 cut(s) 456
BpuMI CCSGG 2 cut(s) 276, 680
BsaHI GRCGYC 2 cut(s) 337, 1026
BsaJI CCNNGG 2 cut(s) 310, 572
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 4 cut(s) 275, 282, 722, 794
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 3 cut(s) 386, 819, 824
BseBI CCWGG 2 cut(s) 573, 723
BseDI CCNNGG 2 cut(s) 310, 572
BseGI GGATG 4 cut(s) 302, 658, 820, 955
BseLI CCNNNNNNNGG 4 cut(s) 275, 282, 722, 794
BseMII CTCAG 2 cut(s) 570, 1023
BseNI ACTGG 3 cut(s) 386, 819, 824
BseXI GCAGC 1 cut(s) 923
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 4 cut(s) 87, 315, 346, 577
BshTI ACCGGT 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 4 cut(s) 261, 276, 347, 679
BslI CCNNNNNNNGG 4 cut(s) 275, 282, 722, 794
BsmAI GTCTC 2 cut(s) 173, 394
BsnI GGCC 4 cut(s) 87, 315, 346, 577
BsoBI CYCGRG 1 cut(s) 208
Bsp143I GATC 4 cut(s) 28, 59, 349, 952
Bsp1720I GCTNAGC 1 cut(s) 456
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 4 cut(s) 163, 246, 358, 966
BspANI GGCC 4 cut(s) 87, 315, 346, 577
BspCNI CTCAG 2 cut(s) 571, 1024
BspHI TCATGA 1 cut(s) 1008
BspLI GGNNCC 1 cut(s) 351
BspPI GGATC 4 cut(s) 36, 344, 357, 947
BspQI GCTCTTC 1 cut(s) 723
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 3 cut(s) 386, 819, 824
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 2 cut(s) 310, 572
BssMI GATC 4 cut(s) 28, 59, 349, 952
BssNI GRCGYC 2 cut(s) 337, 1026
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 310
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 2 cut(s) 573, 723
Bst4CI ACNGT 5 cut(s) 24, 217, 365, 524, 960
Bst6I CTCTTC 3 cut(s) 112, 723, 1035
BstACI GRCGYC 2 cut(s) 337, 1026
BstAPI GCANNNNNTGC 1 cut(s) 871
BstC8I GCNNGC 2 cut(s) 845, 916
BstDEI CTNAG 4 cut(s) 56, 456, 579, 1032
BstDSI CCRYGG 1 cut(s) 310
BstENI CCTNNNNNAGG 1 cut(s) 720
BstF5I GGATG 4 cut(s) 302, 658, 820, 955
BstKTI GATC 4 cut(s) 31, 62, 352, 955
BstMAI GTCTC 2 cut(s) 173, 394
BstMBI GATC 4 cut(s) 28, 59, 349, 952
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 6 cut(s) 334, 343, 762, 871, 920, 991
BstNI CCWGG 2 cut(s) 573, 723
BstNSI RCATGY 1 cut(s) 851
BstSCI CCNGG 4 cut(s) 274, 571, 678, 721
BstSFI CTRYAG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 923
BstV2I GAAGAC 1 cut(s) 525
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 4 cut(s) 87, 315, 346, 577
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 4 cut(s) 302, 658, 820, 955
BtsI GCAGTG 1 cut(s) 838
BtsIMutI CAGTG 4 cut(s) 807, 838, 956, 1029
Cac8I GCNNGC 2 cut(s) 845, 916
CciI TCATGA 1 cut(s) 1008
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 3 cut(s) 344, 575, 697
CseI GACGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 142, 205
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 6 cut(s) 32, 311, 333, 848, 871, 1009
CviQI GTAC 2 cut(s) 142, 205
DdeI CTNAG 4 cut(s) 56, 456, 579, 1032
DpnI GATC 4 cut(s) 30, 61, 351, 954
DpnII GATC 4 cut(s) 28, 59, 349, 952
DriI GACNNNNNGTC 1 cut(s) 386
Eam1104I CTCTTC 3 cut(s) 112, 723, 1035
Eam1105I GACNNNNNGTC 1 cut(s) 386
EarI CTCTTC 3 cut(s) 112, 723, 1035
Eco130I CCWWGG 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 697
Eco57I CTGAAG 2 cut(s) 627, 647
Eco88I CYCGRG 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoRII CCWGG 2 cut(s) 571, 721
EcoT14I CCWWGG 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 876
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 6 cut(s) 35, 314, 336, 851, 874, 1012
FatI CATG 6 cut(s) 31, 310, 332, 847, 870, 1008
FauI CCCGC 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 912, 966
FokI GGATG 4 cut(s) 289, 645, 807, 962
Fsp4HI GCNGC 2 cut(s) 912, 966
GluI GCNGC 2 cut(s) 912, 966
GsuI CTGGAG 1 cut(s) 836
HaeIII GGCC 4 cut(s) 87, 315, 346, 577
HapII CCGG 4 cut(s) 261, 276, 347, 679
HgaI GACGC 1 cut(s) 326
Hin1I GRCGYC 2 cut(s) 337, 1026
Hin1II CATG 6 cut(s) 35, 314, 336, 851, 874, 1012
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 4 cut(s) 41, 101, 548, 997
HpaII CCGG 4 cut(s) 261, 276, 347, 679
HphI GGTGA 2 cut(s) 484, 737
Hpy166II GTNNAC 1 cut(s) 853
Hpy188I TCNGA 5 cut(s) 116, 285, 354, 937, 1002
Hpy188III TCNNGA 5 cut(s) 210, 448, 607, 691, 1009
Hpy8I GTNNAC 1 cut(s) 853
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 6 cut(s) 421, 463, 469, 622, 688, 940
HpyCH4III ACNGT 5 cut(s) 24, 217, 365, 524, 960
HpyCH4IV ACGT 2 cut(s) 670, 1026
HpyCH4V TGCA 5 cut(s) 437, 620, 843, 874, 911
HpyF10VI GCNNNNNNNGC 6 cut(s) 334, 343, 762, 871, 920, 991
HpyF3I CTNAG 4 cut(s) 56, 456, 579, 1032
HpySE526I ACGT 2 cut(s) 670, 1026
Hsp92I GRCGYC 2 cut(s) 337, 1026
Hsp92II CATG 6 cut(s) 35, 314, 336, 851, 874, 1012
Kzo9I GATC 4 cut(s) 28, 59, 349, 952
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 3 cut(s) 232, 478, 817
Lsp1109I GCAGC 1 cut(s) 923
LweI GCATC 3 cut(s) 121, 829, 890
MaeII ACGT 2 cut(s) 670, 1026
MaeIII GTNAC 4 cut(s) 338, 778, 805, 1027
MalI GATC 4 cut(s) 30, 61, 351, 954
MboI GATC 4 cut(s) 28, 59, 349, 952
MboII GAAGA 5 cut(s) 129, 251, 530, 633, 740
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 1 cut(s) 349
MluCI AATT 8 cut(s) 179, 432, 461, 500, 543, 674, 926, 980
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 3 cut(s) 6, 263, 535
Mph1103I ATGCAT 1 cut(s) 876
MroXI GAANNNNTTC 1 cut(s) 413
MseI TTAA 1 cut(s) 791
MslI CAYNNNNRTG 2 cut(s) 36, 746
MspA1I CMGCKG 2 cut(s) 625, 968
MspI CCGG 4 cut(s) 261, 276, 347, 679
MspR9I CCNGG 4 cut(s) 276, 573, 680, 723
MunI CAATTG 1 cut(s) 543
MvaI CCWGG 2 cut(s) 573, 723
MwoI GCNNNNNNNGC 6 cut(s) 334, 343, 762, 871, 920, 991
NciI CCSGG 2 cut(s) 276, 680
NcoI CCATGG 1 cut(s) 310
NdeII GATC 4 cut(s) 28, 59, 349, 952
NlaIII CATG 6 cut(s) 35, 314, 336, 851, 874, 1012
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 4 cut(s) 338, 778, 805, 1027
NsiI ATGCAT 1 cut(s) 876
NspI RCATGY 1 cut(s) 851
PaeR7I CTCGAG 1 cut(s) 208
PagI TCATGA 1 cut(s) 1008
PciSI GCTCTTC 1 cut(s) 723
PcsI WCGNNNNNNNCGW 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 3 cut(s) 41, 548, 997
PfoI TCCNGGA 1 cut(s) 678
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 2 cut(s) 913, 967
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 571, 721
PspGI CCWGG 2 cut(s) 571, 721
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 3 cut(s) 344, 575, 697
PsrI GAACNNNNNNTAC 2 cut(s) 134, 166
PsuI RGATCY 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 2 cut(s) 143, 206
RsaNI GTAC 2 cut(s) 142, 205
RseI CAYNNNNRTG 2 cut(s) 36, 746
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 1 cut(s) 791
SatI GCNGC 2 cut(s) 912, 966
Sau3AI GATC 4 cut(s) 28, 59, 349, 952
Sau96I GGNCC 3 cut(s) 344, 575, 697
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 4 cut(s) 276, 573, 680, 723
SfaNI GCATC 3 cut(s) 121, 829, 890
SfcI CTRYAG 1 cut(s) 196
Sfr274I CTCGAG 1 cut(s) 208
SinI GGWCC 1 cut(s) 697
SlaI CTCGAG 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 36, 746
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
Sse9I AATT 8 cut(s) 179, 432, 461, 500, 543, 674, 926, 980
SsiI CCGC 4 cut(s) 163, 246, 358, 966
StyD4I CCNGG 4 cut(s) 274, 571, 678, 721
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 5 cut(s) 24, 217, 365, 524, 960
TaiI ACGT 2 cut(s) 673, 1029
TaqI TCGA 6 cut(s) 19, 209, 318, 441, 447, 594
TasI AATT 8 cut(s) 179, 432, 461, 500, 543, 674, 926, 980
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 968
TfiI GAWTC 3 cut(s) 41, 548, 997
Tru1I TTAA 1 cut(s) 791
Tru9I TTAA 1 cut(s) 791
TscAI CASTG 4 cut(s) 814, 838, 963, 1036
TseFI GTSAC 4 cut(s) 338, 778, 805, 1027
TseI GCWGC 1 cut(s) 911
Tsp45I GTSAC 4 cut(s) 338, 778, 805, 1027
TspDTI ATGAA 4 cut(s) 54, 440, 531, 993
TspGWI ACGGA 2 cut(s) 389, 777
TspRI CASTG 4 cut(s) 814, 838, 963, 1036
VpaK11BI GGWCC 1 cut(s) 697
XagI CCTNNNNNAGG 1 cut(s) 720
XceI RCATGY 1 cut(s) 851
XhoI CTCGAG 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 413
ZraI GACGTC 1 cut(s) 1027
Zsp2I ATGCAT 1 cut(s) 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.