Rorug05G0131000

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013014
Physical Location & Seq
Forward (+)
11642555 .. 11644353
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0131000.1

Sequence Viewer

Length: 1308 bp
ATGGCCGATGATGATGGTGTTCAACTTCAAGGGAAGGAGCTATATAAGCACCAGTTTAAGCATACTTTCACCACACGCTTGACAGAGATTCTGGCTTTTGATCCCAACTCTGTGGTTTCAGAGAGGGGTGTGATAGAGATTCTGGAGAAGTTAAACCGAGCGGCAATCATGTATGGCCGGAGCTACATTGCCGAAGATGAGTCCGAGTATGTTGATTGCTCTAATGTGATTGGGTTTGGTGTGCCCCTGATTGGCCAGGAGAGGTGTTTCATGCAGTTGCTCAGAGAGTGTGATCCACAATCGGAAGTGGGTAGGAAATTGTGGAACATGAAACACCAAGCAGCTCTTGTTGGTGAAAAAGTCTCGCAGCATCTTATAAGGATGATGGTGGTGATGAACACAGAGAACAAAAAGGAAATGATGAAGAAGTTCAGAGATACTGTGCATCTGTTTCCATTCTTAGTGGATGCTTTTGAATGGCCGGGGAGAAGTGAATATTTTGGAGTAGTGTATAACAGGATCAACGTTCTTATGGAACACGCACGCAAAGAATTCATCCTGCAGGTTAAGAGATCAAGAATGCCAGCTGGGAGTCTGGGATATGTTGAAGACCTTCTCATAAATTTGGACAACAAAAACGAGCGTGATGAATTTCTTGATGACCTCCTTGATCAGATTGTAGAATTTATATCAATGCTGGAGTCTCTGCTGAGCTTTCCACTACTGCCCGATGGGCCAAGGTTGCTCTCTAGAAATATAAGATCAGTAGCTTGGCTGCGTAAGGAGCTTGTGGATATGGAAATAAAGATGCACAACAATATTGGAAAAAGGGTAAGAGAACTGATGGTGAAGTATAAAGGGAATAAGTATTTCAATGATACTGCTGCATTTCTCACGAGAGTGTATTCACAAAGACATGATATGTGGGAGGATTTGGAAAGTGTGGTGAAAGCGGTGAAGCTGGGACCCCAATCCCGAGGGCGAGTCAGACTCCATGTTGGAGGAACTATCATTCAAAAGTACCGGAATAGCGCACTGATTCGGATAGTAGGAATCAACACCAGGAAGGAAGCTGAGTGGTTCACTGGAAAGCACATTGTCTACTTCTACAGAGCTAACGTGAAGAAAAACAACAGCCACTACCGCTGCATTTGGGGAAAGGTTACTAGGCCTCATGATAATAGTGGCATTCTTTGTGCTAGGTTCATCTCCAATTTACTCCCCAGGCATGGTTGCATGAGGGTATATATGTATCGTGCCAGGATCAACTACGAGTTGCACAGGCCGCCTTTCGTCTATGGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

435

Amino Acids

50.96

Weight (kDa)

9.32

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L35Ae PF01247 340 - 416 7.6e-21 Ribosomal protein L35Ae
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
Acc36I ACCTGC 1 cut(s) 553
AccBSI CCGCTC 1 cut(s) 161
AccI GTMKAC 1 cut(s) 1101
AciI CCGC 4 cut(s) 161, 953, 1144, 1286
AclI AACGTT 1 cut(s) 525
AclWI GGATC 4 cut(s) 95, 287, 527, 1271
AcoI YGGCCR 4 cut(s) 3, 175, 253, 479
AcsI RAATTY 4 cut(s) 551, 622, 650, 683
AfaI GTAC 1 cut(s) 1022
AfiI CCNNNNNNNGG 2 cut(s) 251, 1229
AgsI TTSAA 6 cut(s) 23, 29, 476, 608, 874, 1016
AjnI CCWGG 4 cut(s) 255, 1061, 1223, 1259
AleI CACNNNNGTG 1 cut(s) 899
Alw26I GTCTC 3 cut(s) 367, 708, 1296
AlwI GGATC 4 cut(s) 95, 287, 527, 1271
Ama87I CYCGRG 1 cut(s) 975
AoxI GGCC 7 cut(s) 3, 175, 253, 479, 734, 1169, 1283
ApeKI GCWGC 5 cut(s) 341, 367, 775, 884, 1146
ApoI RAATTY 4 cut(s) 551, 622, 650, 683
Asp700I GAANNNNTTC 2 cut(s) 428, 612
AspLEI GCGC 1 cut(s) 1034
AspS9I GGNCC 2 cut(s) 734, 965
AsuC2I CCSGG 1 cut(s) 483
AsuHPI GGTGA 6 cut(s) 61, 365, 403, 859, 958, 967
AvaI CYCGRG 1 cut(s) 975
AvaII GGWCC 1 cut(s) 965
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 255
BauI CACGAG 1 cut(s) 895
BbsI GAAGAC 1 cut(s) 615
BbvI GCAGC 5 cut(s) 353, 379, 762, 871, 1133
BccI CCATC 4 cut(s) 8, 379, 725, 838
BciT130I CCWGG 4 cut(s) 257, 1063, 1225, 1261
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 1 cut(s) 483
BcoDI GTCTC 3 cut(s) 367, 708, 1296
BfaI CTAG 3 cut(s) 750, 1167, 1200
BfmI CTRYAG 2 cut(s) 560, 1108
BfuAI ACCTGC 1 cut(s) 553
BglI GCCNNNNNGGC 1 cut(s) 733
BisI GCNGC 7 cut(s) 162, 342, 368, 776, 885, 1147, 1286
BlpI GCTNAGC 1 cut(s) 710
BlsI GCNGC 7 cut(s) 163, 343, 369, 777, 886, 1148, 1287
Bme1390I CCNGG 5 cut(s) 257, 483, 1063, 1225, 1261
Bme18I GGWCC 1 cut(s) 965
BmeT110I CYCGRG 1 cut(s) 975
BmgT120I GGNCC 2 cut(s) 734, 965
BmiI GGNNCC 2 cut(s) 966, 967
BmrFI CCNGG 5 cut(s) 257, 483, 1063, 1225, 1261
BmsI GCATC 4 cut(s) 379, 454, 457, 798
BpiI GAAGAC 1 cut(s) 615
BplI GAGNNNNNCTC 2 cut(s) 975, 1007
BpmI CTGGAG 2 cut(s) 164, 719
Bpu1102I GCTNAGC 1 cut(s) 710
BpuMI CCSGG 1 cut(s) 483
BsaJI CCNNGG 4 cut(s) 482, 737, 976, 1223
BsaWI WCCGGW 1 cut(s) 1023
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 1229
Bse1I ACTGG 2 cut(s) 52, 1090
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 4 cut(s) 257, 1063, 1225, 1261
BseDI CCNNGG 4 cut(s) 482, 737, 976, 1223
BseGI GGATG 3 cut(s) 387, 472, 555
BseLI CCNNNNNNNGG 2 cut(s) 251, 1229
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 3 cut(s) 295, 701, 1065
BseNI ACTGG 2 cut(s) 52, 1090
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 5 cut(s) 353, 379, 762, 871, 1133
BseYI CCCAGC 2 cut(s) 587, 961
BshFI GGCC 7 cut(s) 5, 177, 255, 481, 736, 1171, 1285
BsiHKCI CYCGRG 1 cut(s) 975
BsiSI CCGG 3 cut(s) 178, 482, 1024
BslFI GGGAC 1 cut(s) 978
BslI CCNNNNNNNGG 2 cut(s) 251, 1229
BsmAI GTCTC 3 cut(s) 367, 708, 1296
BsmFI GGGAC 1 cut(s) 978
BsmI GAATGC 2 cut(s) 585, 1188
BsnI GGCC 7 cut(s) 5, 177, 255, 481, 736, 1171, 1285
BsoBI CYCGRG 1 cut(s) 975
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 7 cut(s) 100, 292, 519, 572, 670, 761, 1263
Bsp1720I GCTNAGC 1 cut(s) 710
BspACI CCGC 4 cut(s) 161, 953, 1144, 1286
BspANI GGCC 7 cut(s) 5, 177, 255, 481, 736, 1171, 1285
BspCNI CTCAG 3 cut(s) 294, 702, 1066
BspHI TCATGA 1 cut(s) 1174
BspLI GGNNCC 2 cut(s) 966, 967
BspMAI CTGCAG 1 cut(s) 564
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 4 cut(s) 95, 287, 527, 1271
BsrBI CCGCTC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 186
BsrI ACTGG 2 cut(s) 52, 1090
BssECI CCNNGG 4 cut(s) 482, 737, 976, 1223
BssMI GATC 7 cut(s) 100, 292, 519, 572, 670, 761, 1263
BssSI CACGAG 1 cut(s) 895
BssT1I CCWWGG 1 cut(s) 737
Bst2BI CACGAG 1 cut(s) 895
Bst2UI CCWGG 4 cut(s) 257, 1063, 1225, 1261
Bst4CI ACNGT 1 cut(s) 442
BstC8I GCNNGC 2 cut(s) 544, 585
BstDEI CTNAG 4 cut(s) 281, 460, 710, 1074
BstF5I GGATG 3 cut(s) 387, 472, 555
BstHHI GCGC 1 cut(s) 1034
BstKTI GATC 7 cut(s) 103, 295, 522, 575, 673, 764, 1266
BstMAI GTCTC 3 cut(s) 367, 708, 1296
BstMBI GATC 7 cut(s) 100, 292, 519, 572, 670, 761, 1263
BstMWI GCNNNNNNNGC 6 cut(s) 46, 733, 742, 784, 1143, 1285
BstNI CCWGG 4 cut(s) 257, 1063, 1225, 1261
BstSCI CCNGG 5 cut(s) 255, 481, 1061, 1223, 1259
BstSFI CTRYAG 2 cut(s) 560, 1108
BstSLI GKGCMC 1 cut(s) 246
BstV1I GCAGC 5 cut(s) 353, 379, 762, 871, 1133
BstV2I GAAGAC 1 cut(s) 615
BstXI CCANNNNNNTGG 1 cut(s) 112
BsuRI GGCC 7 cut(s) 5, 177, 255, 481, 736, 1171, 1285
BtsCI GGATG 3 cut(s) 387, 472, 555
BtsIMutI CAGTG 2 cut(s) 1034, 1083
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 2 cut(s) 544, 585
CciI TCATGA 1 cut(s) 1174
CfoI GCGC 1 cut(s) 1034
Cfr13I GGNCC 2 cut(s) 734, 965
Csp6I GTAC 1 cut(s) 1021
CviAII CATG 8 cut(s) 169, 271, 328, 917, 995, 1175, 1229, 1237
CviQI GTAC 1 cut(s) 1021
DdeI CTNAG 4 cut(s) 281, 460, 710, 1074
DpnI GATC 7 cut(s) 102, 294, 521, 574, 672, 763, 1265
DpnII GATC 7 cut(s) 100, 292, 519, 572, 670, 761, 1263
EaeI YGGCCR 4 cut(s) 3, 175, 253, 479
Eco130I CCWWGG 1 cut(s) 737
Eco147I AGGCCT 1 cut(s) 1171
Eco47I GGWCC 1 cut(s) 965
Eco88I CYCGRG 1 cut(s) 975
EcoO109I RGGNCCY 1 cut(s) 965
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 4 cut(s) 255, 1061, 1223, 1259
EcoT14I CCWWGG 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 737
FaeI CATG 8 cut(s) 172, 274, 331, 920, 998, 1178, 1232, 1240
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FaqI GGGAC 1 cut(s) 978
FatI CATG 8 cut(s) 168, 270, 327, 916, 994, 1174, 1228, 1236
FbaI TGATCA 1 cut(s) 670
FblI GTMKAC 1 cut(s) 1101
Fnu4HI GCNGC 7 cut(s) 162, 342, 368, 776, 885, 1147, 1286
FokI GGATG 3 cut(s) 394, 479, 542
Fsp4HI GCNGC 7 cut(s) 162, 342, 368, 776, 885, 1147, 1286
FspBI CTAG 3 cut(s) 750, 1167, 1200
GlaI GCGC 1 cut(s) 1033
GluI GCNGC 7 cut(s) 162, 342, 368, 776, 885, 1147, 1286
GsaI CCCAGC 2 cut(s) 591, 965
GsuI CTGGAG 2 cut(s) 164, 719
HaeIII GGCC 7 cut(s) 5, 177, 255, 481, 736, 1171, 1285
HapII CCGG 3 cut(s) 178, 482, 1024
HhaI GCGC 1 cut(s) 1034
Hin1II CATG 8 cut(s) 172, 274, 331, 920, 998, 1178, 1232, 1240
Hin6I GCGC 1 cut(s) 1032
HinP1I GCGC 1 cut(s) 1032
HinfI GANTC 9 cut(s) 88, 139, 200, 592, 701, 984, 990, 1039, 1053
HpaII CCGG 3 cut(s) 178, 482, 1024
HphI GGTGA 6 cut(s) 61, 365, 403, 859, 958, 967
Hpy166II GTNNAC 2 cut(s) 1083, 1102
Hpy188I TCNGA 8 cut(s) 121, 205, 284, 304, 434, 675, 989, 1044
Hpy188III TCNNGA 7 cut(s) 143, 576, 656, 750, 895, 975, 1175
Hpy8I GTNNAC 2 cut(s) 1083, 1102
HpyAV CCTTC 3 cut(s) 28, 623, 1060
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 2 cut(s) 525, 1119
HpyCH4V TGCA 8 cut(s) 274, 445, 562, 811, 887, 1149, 1236, 1279
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 733, 742, 784, 1143, 1285
HpyF3I CTNAG 4 cut(s) 281, 460, 710, 1074
HpySE526I ACGT 2 cut(s) 525, 1119
Hsp92II CATG 8 cut(s) 172, 274, 331, 920, 998, 1178, 1232, 1240
HspAI GCGC 1 cut(s) 1032
KflI GGGWCCC 1 cut(s) 965
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 7 cut(s) 100, 292, 519, 572, 670, 761, 1263
LmnI GCTCC 3 cut(s) 37, 180, 784
Lsp1109I GCAGC 5 cut(s) 353, 379, 762, 871, 1133
LweI GCATC 4 cut(s) 379, 454, 457, 798
MaeI CTAG 3 cut(s) 750, 1167, 1200
MaeII ACGT 2 cut(s) 525, 1119
MaeIII GTNAC 1 cut(s) 1162
MalI GATC 7 cut(s) 102, 294, 521, 574, 672, 763, 1265
MbiI CCGCTC 1 cut(s) 161
MboI GATC 7 cut(s) 100, 292, 519, 572, 670, 761, 1263
MboII GAAGA 4 cut(s) 206, 436, 620, 1135
MhlI GDGCHC 1 cut(s) 246
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 6 cut(s) 317, 551, 622, 650, 683, 1213
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 5 cut(s) 209, 601, 710, 984, 993
MmeI TCCRAC 1 cut(s) 979
MnlI CCTC 8 cut(s) 117, 255, 674, 922, 971, 995, 1182, 1233
Mox20I TGGCCA 1 cut(s) 255
MroXI GAANNNNTTC 2 cut(s) 428, 612
MscI TGGCCA 1 cut(s) 255
MseI TTAA 3 cut(s) 57, 152, 567
MslI CAYNNNNRTG 1 cut(s) 899
Msp20I TGGCCA 1 cut(s) 255
MspA1I CMGCKG 2 cut(s) 587, 1146
MspI CCGG 3 cut(s) 178, 482, 1024
MspR9I CCNGG 5 cut(s) 257, 483, 1063, 1225, 1261
Mva1269I GAATGC 2 cut(s) 585, 1188
MvaI CCWGG 4 cut(s) 257, 1063, 1225, 1261
MwoI GCNNNNNNNGC 6 cut(s) 46, 733, 742, 784, 1143, 1285
NciI CCSGG 1 cut(s) 483
NdeII GATC 7 cut(s) 100, 292, 519, 572, 670, 761, 1263
NlaIII CATG 8 cut(s) 172, 274, 331, 920, 998, 1178, 1232, 1240
NlaIV GGNNCC 2 cut(s) 966, 967
OliI CACNNNNGTG 1 cut(s) 899
PagI TCATGA 1 cut(s) 1174
PceI AGGCCT 1 cut(s) 1171
PctI GAATGC 2 cut(s) 585, 1188
PdmI GAANNNNTTC 2 cut(s) 428, 612
PfeI GAWTC 4 cut(s) 88, 139, 1039, 1053
PkrI GCNGC 7 cut(s) 163, 343, 369, 777, 886, 1148, 1287
PleI GAGTC 5 cut(s) 208, 600, 709, 984, 992
PpsI GAGTC 5 cut(s) 208, 600, 709, 984, 992
PpuMI RGGWCCY 1 cut(s) 965
PsiI TTATAA 1 cut(s) 377
Psp1406I AACGTT 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 965
Psp6I CCWGG 4 cut(s) 255, 1061, 1223, 1259
PspFI CCCAGC 2 cut(s) 587, 961
PspGI CCWGG 4 cut(s) 255, 1061, 1223, 1259
PspN4I GGNNCC 2 cut(s) 966, 967
PspPI GGNCC 2 cut(s) 734, 965
PspPPI RGGWCCY 1 cut(s) 965
PstI CTGCAG 1 cut(s) 564
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 1 cut(s) 1022
RsaNI GTAC 1 cut(s) 1021
RseI CAYNNNNRTG 1 cut(s) 899
SaqAI TTAA 3 cut(s) 57, 152, 567
SatI GCNGC 7 cut(s) 162, 342, 368, 776, 885, 1147, 1286
Sau3AI GATC 7 cut(s) 100, 292, 519, 572, 670, 761, 1263
Sau96I GGNCC 2 cut(s) 734, 965
SbfI CCTGCAGG 1 cut(s) 564
SchI GAGTC 5 cut(s) 209, 601, 710, 984, 993
ScrFI CCNGG 5 cut(s) 257, 483, 1063, 1225, 1261
SdaI CCTGCAGG 1 cut(s) 564
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 4 cut(s) 379, 454, 457, 798
SfcI CTRYAG 2 cut(s) 560, 1108
SinI GGWCC 1 cut(s) 965
SmiMI CAYNNNNRTG 1 cut(s) 899
Sse8387I CCTGCAGG 1 cut(s) 564
Sse9I AATT 6 cut(s) 317, 551, 622, 650, 683, 1213
SseBI AGGCCT 1 cut(s) 1171
SsiI CCGC 4 cut(s) 161, 953, 1144, 1286
SspI AATATT 2 cut(s) 497, 820
SspMI CTAG 3 cut(s) 750, 1167, 1200
StuI AGGCCT 1 cut(s) 1171
StyD4I CCNGG 5 cut(s) 255, 481, 1061, 1223, 1259
StyI CCWWGG 1 cut(s) 737
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 2 cut(s) 528, 1122
TasI AATT 6 cut(s) 317, 551, 622, 650, 683, 1213
TauI GCSGC 2 cut(s) 164, 1288
TfiI GAWTC 4 cut(s) 88, 139, 1039, 1053
Tru1I TTAA 3 cut(s) 57, 152, 567
Tru9I TTAA 3 cut(s) 57, 152, 567
TscAI CASTG 2 cut(s) 1041, 1090
TseI GCWGC 5 cut(s) 341, 367, 775, 884, 1146
TspDTI ATGAA 7 cut(s) 259, 344, 410, 437, 544, 663, 1195
TspRI CASTG 2 cut(s) 1041, 1090
VpaK11BI GGWCC 1 cut(s) 965
XapI RAATTY 4 cut(s) 551, 622, 650, 683
XbaI TCTAGA 1 cut(s) 749
XmiI GTMKAC 1 cut(s) 1101
XmnI GAANNNNTTC 2 cut(s) 428, 612
XspI CTAG 3 cut(s) 750, 1167, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.