RLG00000033386

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013014
Physical Location & Seq
Reverse (-)
26508101 .. 26511404
3304 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033386

Sequence Viewer

Length: 765 bp
ATGGCCTCGACCTCCTCTCGGACTCATGGCATCGCCGGTCGGATCCGAACCGCCGCAGCCAATCTTTACTCCGACAACCAGTCCCTGCTCGGCGATATTCGAAAATCTTTCAATGTGTTGAAGGAAGTTGCTGTTGATTTGGAGAGAGCTAATCAGTTTGACAAGGTGAAGGAGATTGAAAATGCTGCTGTTGAATTGCTGGCTACTTGTGAACACTGTATGCATCTCTCATCAGCAATTCAATATGTTGGAGATAACTACGAACCTCCTCCAGAGCCTAGTCAGCTTACCGATTTTGAGAAGTTGCTTAAAAATGAGGTTGCAAAGCAAAAGGCTAAATCATCTTCAACTACAGTAAACCAGTCTATGATACGTCAATTCAGGGAAGCTGTCTGGAATGTTCATCATGCAGGACAGCCTATGCCAGGTGACGACCAGGAGGACATCGTAATGACTAGTACTGATGGCAATCTTCGGAATACCACTTGCCCAGTAAGTGGAAAGGCTATCACGGATTTGGAAGATCCAGTCTGCAGTGTTGACTGCAAGCATATATATCAAACAAAGGAGATTATGCATTACTTGCAGTTAAAGGGGGGACGTGGACCATGCCCAATTGCAGGTTGTCCTAAGATGTTGCAGGTGGACAAATTGGTTCGTGATCCATTGCTACTAGTTGAAATTCAAGAATTGCGCGATATGAGGAAGCACCAGACCGCTGATACTAACGTGATAGAGGATTTCACTGAGCTGGATGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

255

Amino Acids

28.35

Weight (kDa)

5.22

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Nse PF11789 148 - 209 2.2e-08 Zinc-finger of the MIZ type in Nse subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 631
Acc36I ACCTGC 2 cut(s) 611, 631
AccB7I CCANNNNNTGG 1 cut(s) 497
AccII CGCG 1 cut(s) 696
AciI CCGC 3 cut(s) 51, 54, 717
AclWI GGATC 4 cut(s) 37, 50, 518, 656
AcsI RAATTY 1 cut(s) 681
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 4 cut(s) 18, 425, 497, 620
AgsI TTSAA 8 cut(s) 112, 121, 179, 194, 242, 348, 680, 686
AhdI GACNNNNNGTC 1 cut(s) 79
AhlI ACTAGT 2 cut(s) 455, 673
AjiI CACGTC 1 cut(s) 602
AjnI CCWGG 2 cut(s) 424, 435
AluBI AGCT 4 cut(s) 149, 286, 389, 751
AluI AGCT 4 cut(s) 149, 286, 389, 751
AlwI GGATC 4 cut(s) 37, 50, 518, 656
AlwNI CAGNNNCTG 1 cut(s) 85
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 56, 185
ApoI RAATTY 1 cut(s) 681
AspLEI GCGC 1 cut(s) 696
AspS9I GGNCC 1 cut(s) 605
AsuHPI GGTGA 2 cut(s) 178, 440
AsuII TTCGAA 1 cut(s) 100
AvaII GGWCC 1 cut(s) 605
BaeI ACNNNNGTAYC 2 cut(s) 714, 747
BamHI GGATCC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 68, 172
BccI CCATC 1 cut(s) 458
BciT130I CCWGG 2 cut(s) 426, 437
BcuI ACTAGT 2 cut(s) 455, 673
BfaI CTAG 3 cut(s) 279, 456, 674
BfmI CTRYAG 2 cut(s) 351, 532
BfuAI ACCTGC 2 cut(s) 611, 631
BisI GCNGC 3 cut(s) 54, 57, 186
BlsI GCNGC 3 cut(s) 55, 58, 187
BmcAI AGTACT 1 cut(s) 460
Bme1390I CCNGG 2 cut(s) 426, 437
Bme18I GGWCC 1 cut(s) 605
BmeRI GACNNNNNGTC 1 cut(s) 79
BmgBI CACGTC 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 2 cut(s) 426, 437
BmrI ACTGGG 1 cut(s) 485
BmsI GCATC 2 cut(s) 39, 232
BmuI ACTGGG 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 255
Bpu14I TTCGAA 1 cut(s) 100
BsaBI GATNNNNATC 1 cut(s) 468
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 425, 497, 620
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 4 cut(s) 79, 361, 491, 527
Bse3DI GCAATG 1 cut(s) 665
Bse8I GATNNNNATC 1 cut(s) 468
BseBI CCWGG 2 cut(s) 426, 437
BseGI GGATG 1 cut(s) 760
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 4 cut(s) 18, 425, 497, 620
BseMI GCAATG 1 cut(s) 665
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 4 cut(s) 79, 361, 491, 527
BseRI GAGGAG 2 cut(s) 4, 258
BseXI GCAGC 2 cut(s) 68, 172
Bsh1236I CGCG 1 cut(s) 696
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 2 cut(s) 67, 612
BslI CCNNNNNNNGG 4 cut(s) 18, 425, 497, 620
BsmFI GGGAC 2 cut(s) 67, 612
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 100
Bsp143I GATC 3 cut(s) 42, 523, 661
BspACI CCGC 3 cut(s) 51, 54, 717
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 739
BspFNI CGCG 1 cut(s) 696
BspLI GGNNCC 1 cut(s) 44
BspMAI CTGCAG 1 cut(s) 536
BspMI ACCTGC 2 cut(s) 611, 631
BspPI GGATC 4 cut(s) 37, 50, 518, 656
BspT104I TTCGAA 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 665
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 4 cut(s) 79, 361, 491, 527
BssAI RCCGGY 1 cut(s) 35
BssMI GATC 3 cut(s) 42, 523, 661
Bst2UI CCWGG 2 cut(s) 426, 437
Bst4CI ACNGT 2 cut(s) 218, 355
BstAPI GCANNNNNTGC 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 100
BstC8I GCNNGC 2 cut(s) 201, 548
BstDEI CTNAG 2 cut(s) 630, 747
BstENI CCTNNNNNAGG 1 cut(s) 423
BstF5I GGATG 1 cut(s) 760
BstFNI CGCG 1 cut(s) 696
BstHHI GCGC 1 cut(s) 696
BstKTI GATC 3 cut(s) 45, 526, 664
BstMBI GATC 3 cut(s) 42, 523, 661
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 283, 583
BstNI CCWGG 2 cut(s) 426, 437
BstSCI CCNGG 2 cut(s) 424, 435
BstSFI CTRYAG 2 cut(s) 351, 532
BstUI CGCG 1 cut(s) 696
BstV1I GCAGC 2 cut(s) 68, 172
BstX2I RGATCY 2 cut(s) 42, 523
BstYI RGATCY 2 cut(s) 42, 523
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 16
BtrI CACGTC 1 cut(s) 602
BtsCI GGATG 1 cut(s) 760
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 3 cut(s) 214, 541, 744
BveI ACCTGC 2 cut(s) 611, 631
Cac8I GCNNGC 2 cut(s) 201, 548
CaiI CAGNNNCTG 1 cut(s) 85
CfoI GCGC 1 cut(s) 696
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 605
Csp6I GTAC 1 cut(s) 459
CviAII CATG 3 cut(s) 26, 407, 609
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 2 cut(s) 630, 747
DpnI GATC 3 cut(s) 44, 525, 663
DpnII GATC 3 cut(s) 42, 523, 661
DriI GACNNNNNGTC 1 cut(s) 79
Eam1105I GACNNNNNGTC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 605
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoRII CCWGG 2 cut(s) 424, 435
EcoT22I ATGCAT 2 cut(s) 225, 579
FaeI CATG 3 cut(s) 29, 410, 612
FaqI GGGAC 2 cut(s) 67, 612
FatI CATG 3 cut(s) 25, 406, 608
Fnu4HI GCNGC 3 cut(s) 54, 57, 186
Fsp4HI GCNGC 3 cut(s) 54, 57, 186
FspBI CTAG 3 cut(s) 279, 456, 674
GlaI GCGC 1 cut(s) 695
GluI GCNGC 3 cut(s) 54, 57, 186
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 696
Hin1II CATG 3 cut(s) 29, 410, 612
Hin6I GCGC 1 cut(s) 694
HinP1I GCGC 1 cut(s) 694
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 1 cut(s) 22
HpaII CCGG 1 cut(s) 36
HphI GGTGA 2 cut(s) 178, 440
Hpy166II GTNNAC 5 cut(s) 212, 358, 541, 605, 646
Hpy188I TCNGA 5 cut(s) 21, 42, 47, 73, 477
Hpy188III TCNNGA 4 cut(s) 272, 394, 659, 686
Hpy8I GTNNAC 5 cut(s) 212, 358, 541, 605, 646
HpyAV CCTTC 2 cut(s) 115, 163
HpyCH4III ACNGT 2 cut(s) 218, 355
HpyCH4IV ACGT 3 cut(s) 373, 601, 729
HpyCH4V TGCA 9 cut(s) 223, 323, 410, 534, 546, 577, 586, 620, 640
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 583
HpyF3I CTNAG 2 cut(s) 630, 747
HpySE526I ACGT 3 cut(s) 373, 601, 729
Hsp92II CATG 3 cut(s) 29, 410, 612
HspAI GCGC 1 cut(s) 694
Kzo9I GATC 3 cut(s) 42, 523, 661
Lsp1109I GCAGC 2 cut(s) 68, 172
LweI GCATC 2 cut(s) 39, 232
MaeI CTAG 3 cut(s) 279, 456, 674
MaeII ACGT 3 cut(s) 373, 601, 729
MaeIII GTNAC 1 cut(s) 428
MalI GATC 3 cut(s) 44, 525, 663
MboI GATC 3 cut(s) 42, 523, 661
MboII GAAGA 3 cut(s) 336, 464, 533
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 2 cut(s) 42, 523
MluCI AATT 7 cut(s) 194, 237, 377, 615, 650, 681, 689
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 3 cut(s) 20, 96, 229
MnlI CCTC 9 cut(s) 16, 22, 25, 276, 279, 310, 433, 696, 730
Mph1103I ATGCAT 2 cut(s) 225, 579
MseI TTAA 2 cut(s) 309, 590
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 426, 437
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 2 cut(s) 426, 437
MvnI CGCG 1 cut(s) 696
MwoI GCNNNNNNNGC 2 cut(s) 283, 583
NdeII GATC 3 cut(s) 42, 523, 661
NlaIII CATG 3 cut(s) 29, 410, 612
NlaIV GGNNCC 1 cut(s) 44
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 428
NsiI ATGCAT 2 cut(s) 225, 579
NspV TTCGAA 1 cut(s) 100
PaqCI CACCTGC 1 cut(s) 631
PflMI CCANNNNNTGG 1 cut(s) 497
PkrI GCNGC 3 cut(s) 55, 58, 187
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 2 cut(s) 424, 435
PspGI CCWGG 2 cut(s) 424, 435
PspN4I GGNNCC 1 cut(s) 44
PspPI GGNCC 1 cut(s) 605
PstI CTGCAG 1 cut(s) 536
PstNI CAGNNNCTG 1 cut(s) 85
PsuI RGATCY 2 cut(s) 42, 523
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 2 cut(s) 309, 590
SatI GCNGC 3 cut(s) 54, 57, 186
Sau3AI GATC 3 cut(s) 42, 523, 661
Sau96I GGNCC 1 cut(s) 605
ScaI AGTACT 1 cut(s) 460
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 426, 437
SfaNI GCATC 2 cut(s) 39, 232
SfcI CTRYAG 2 cut(s) 351, 532
SfuI TTCGAA 1 cut(s) 100
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 1 cut(s) 449
SpeI ACTAGT 2 cut(s) 455, 673
Sse9I AATT 7 cut(s) 194, 237, 377, 615, 650, 681, 689
SsiI CCGC 3 cut(s) 51, 54, 717
SspMI CTAG 3 cut(s) 279, 456, 674
StyD4I CCNGG 2 cut(s) 424, 435
TaaI ACNGT 2 cut(s) 218, 355
TaiI ACGT 3 cut(s) 376, 604, 732
TaqI TCGA 2 cut(s) 8, 100
TasI AATT 7 cut(s) 194, 237, 377, 615, 650, 681, 689
TatI WGTACW 1 cut(s) 458
TauI GCSGC 1 cut(s) 56
Tru1I TTAA 2 cut(s) 309, 590
Tru9I TTAA 2 cut(s) 309, 590
TscAI CASTG 3 cut(s) 221, 541, 751
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 2 cut(s) 56, 185
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 1 cut(s) 392
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 3 cut(s) 221, 541, 751
Van91I CCANNNNNTGG 1 cut(s) 497
VpaK11BI GGWCC 1 cut(s) 605
XagI CCTNNNNNAGG 1 cut(s) 423
XapI RAATTY 1 cut(s) 681
XspI CTAG 3 cut(s) 279, 456, 674
ZrmI AGTACT 1 cut(s) 460
Zsp2I ATGCAT 2 cut(s) 225, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.