Rh5CG250800

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013014
Physical Location & Seq
Reverse (-)
26390465 .. 26393500
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG250800.1

Sequence Viewer

Length: 660 bp
ATGTCAGAGGGACAAGATAGGAGTCTTTCCTCACCATATGAAATTCTGAATTTTGAACAGGTGAAGGAGATTGAAAATGCTGCTGTTGAATTGCTGGCTACTTGTGAACACTGTATGCATCTCTCATCAGCAATTCAATATGTTGGAGATAACTACGAACCCCCTCCAGAGCCTAGTCAGCTTACTGATTTTGAGAAGTTGCTCAAAAATGAGGTTGCAAAGCAAAAGGCTAAATCATCTTCAACTACAGTAAACCATTCTATGATACGTCAATTCCGGGAAGCTGTCTGGAATGTTCATCATGCAGGACAGCCTATGCCAGGTGACGACCAGGAGGACATCGTAATGACTAGTACTGATGGCAATCTTCGGAATACCACTTGCCCAGTAAGTGGAAAGGCTATCACGGATTTGGAAGATCCAGTCTGCAGTGTTGACTGCAAGCATATATATCAAACAAAGGAGATTATGCATTACTTGCAGTTAAAGGGGGGACGTGGACCATGCCCAATTGCAGGTTGTCCTAAGATGTTGCAGGCGGACAAATTGGTTCGTGATCCATTGCTACTAGTTGAAATTCAAGAATTGCGCGATATGAGGAAGCACCAGACCGCTGATACTAACGTGATAGAGGATTTCACTGTTCTGGATGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

219

Amino Acids

24.6

Weight (kDa)

4.88

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Nse PF11789 113 - 174 1.7e-08 Zinc-finger of the MIZ type in Nse subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 506
AccB7I CCANNNNNTGG 1 cut(s) 392
AccII CGCG 1 cut(s) 591
AciI CCGC 2 cut(s) 539, 612
AclWI GGATC 2 cut(s) 413, 551
AcsI RAATTY 3 cut(s) 42, 49, 576
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 3 cut(s) 320, 392, 515
AgsI TTSAA 7 cut(s) 56, 74, 89, 137, 243, 575, 581
AhlI ACTAGT 2 cut(s) 350, 568
AjiI CACGTC 1 cut(s) 497
AjnI CCWGG 2 cut(s) 319, 330
AluBI AGCT 2 cut(s) 181, 284
AluI AGCT 2 cut(s) 181, 284
AlwI GGATC 2 cut(s) 413, 551
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 3 cut(s) 42, 49, 576
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 1 cut(s) 500
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 3 cut(s) 24, 73, 335
AvaII GGWCC 1 cut(s) 500
BaeI ACNNNNGTAYC 2 cut(s) 609, 642
BbvI GCAGC 1 cut(s) 67
BccI CCATC 1 cut(s) 353
BciT130I CCWGG 2 cut(s) 321, 332
BcnI CCSGG 1 cut(s) 278
BcuI ACTAGT 2 cut(s) 350, 568
BfaI CTAG 3 cut(s) 174, 351, 569
BfmI CTRYAG 2 cut(s) 246, 427
BfuAI ACCTGC 1 cut(s) 506
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
BmcAI AGTACT 1 cut(s) 355
Bme1390I CCNGG 3 cut(s) 278, 321, 332
Bme18I GGWCC 1 cut(s) 500
BmgBI CACGTC 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 500
BmrFI CCNGG 3 cut(s) 278, 321, 332
BmrI ACTGGG 1 cut(s) 380
BmsI GCATC 1 cut(s) 127
BmuI ACTGGG 1 cut(s) 380
BpmI CTGGAG 1 cut(s) 150
BpuMI CCSGG 1 cut(s) 278
BsaBI GATNNNNATC 1 cut(s) 363
Bsc4I CCNNNNNNNGG 3 cut(s) 320, 392, 515
Bse1I ACTGG 2 cut(s) 386, 422
Bse3DI GCAATG 1 cut(s) 560
Bse8I GATNNNNATC 1 cut(s) 363
BseBI CCWGG 2 cut(s) 321, 332
BseGI GGATG 1 cut(s) 655
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 3 cut(s) 320, 392, 515
BseMI GCAATG 1 cut(s) 560
BseNI ACTGG 2 cut(s) 386, 422
BseXI GCAGC 1 cut(s) 67
Bsh1236I CGCG 1 cut(s) 591
BsiSI CCGG 1 cut(s) 277
BslFI GGGAC 2 cut(s) 24, 507
BslI CCNNNNNNNGG 3 cut(s) 320, 392, 515
BsmFI GGGAC 2 cut(s) 24, 507
Bsp143I GATC 2 cut(s) 418, 556
BspACI CCGC 2 cut(s) 539, 612
BspFNI CGCG 1 cut(s) 591
BspMAI CTGCAG 1 cut(s) 431
BspMI ACCTGC 1 cut(s) 506
BspPI GGATC 2 cut(s) 413, 551
BsrDI GCAATG 1 cut(s) 560
BsrI ACTGG 2 cut(s) 386, 422
BssMI GATC 2 cut(s) 418, 556
Bst2UI CCWGG 2 cut(s) 321, 332
Bst4CI ACNGT 3 cut(s) 113, 250, 643
BstAPI GCANNNNNTGC 1 cut(s) 478
BstC8I GCNNGC 3 cut(s) 96, 443, 537
BstDEI CTNAG 1 cut(s) 525
BstENI CCTNNNNNAGG 1 cut(s) 318
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 1 cut(s) 591
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 2 cut(s) 421, 559
BstMBI GATC 2 cut(s) 418, 556
BstMWI GCNNNNNNNGC 2 cut(s) 178, 478
BstNI CCWGG 2 cut(s) 321, 332
BstSCI CCNGG 3 cut(s) 276, 319, 330
BstSFI CTRYAG 2 cut(s) 246, 427
BstUI CGCG 1 cut(s) 591
BstV1I GCAGC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BtrI CACGTC 1 cut(s) 497
BtsCI GGATG 1 cut(s) 655
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 109, 436, 639
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 3 cut(s) 96, 443, 537
CfoI GCGC 1 cut(s) 591
Cfr13I GGNCC 1 cut(s) 500
Csp6I GTAC 1 cut(s) 354
CviAII CATG 2 cut(s) 302, 504
CviJI RGCY 7 cut(s) 98, 172, 181, 230, 284, 313, 401
CviKI_1 RGCY 7 cut(s) 98, 172, 181, 230, 284, 313, 401
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 1 cut(s) 525
DpnI GATC 2 cut(s) 420, 558
DpnII GATC 2 cut(s) 418, 556
EciI GGCGGA 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 500
EcoNI CCTNNNNNAGG 1 cut(s) 318
EcoRII CCWGG 2 cut(s) 319, 330
EcoT22I ATGCAT 2 cut(s) 120, 474
FaeI CATG 2 cut(s) 305, 507
FaqI GGGAC 2 cut(s) 24, 507
FatI CATG 2 cut(s) 301, 503
FauNDI CATATG 1 cut(s) 37
Fnu4HI GCNGC 1 cut(s) 81
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 3 cut(s) 174, 351, 569
GlaI GCGC 1 cut(s) 590
GluI GCNGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 150
HapII CCGG 1 cut(s) 277
HhaI GCGC 1 cut(s) 591
Hin1II CATG 2 cut(s) 305, 507
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 1 cut(s) 22
HpaII CCGG 1 cut(s) 277
HphI GGTGA 3 cut(s) 24, 73, 335
Hpy166II GTNNAC 4 cut(s) 107, 253, 436, 500
Hpy188I TCNGA 3 cut(s) 7, 48, 372
Hpy188III TCNNGA 5 cut(s) 167, 289, 554, 581, 647
Hpy8I GTNNAC 4 cut(s) 107, 253, 436, 500
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 3 cut(s) 113, 250, 643
HpyCH4IV ACGT 3 cut(s) 268, 496, 624
HpyCH4V TGCA 9 cut(s) 118, 218, 305, 429, 441, 472, 481, 515, 535
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 478
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 3 cut(s) 268, 496, 624
Hsp92II CATG 2 cut(s) 305, 507
HspAI GCGC 1 cut(s) 589
Kzo9I GATC 2 cut(s) 418, 556
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 1 cut(s) 127
MaeI CTAG 3 cut(s) 174, 351, 569
MaeII ACGT 3 cut(s) 268, 496, 624
MaeIII GTNAC 1 cut(s) 323
MalI GATC 2 cut(s) 420, 558
MboI GATC 2 cut(s) 418, 556
MboII GAAGA 3 cut(s) 231, 359, 428
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 1 cut(s) 418
MluCI AATT 9 cut(s) 42, 49, 89, 132, 272, 510, 545, 576, 584
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 1 cut(s) 124
MnlI CCTC 6 cut(s) 40, 174, 205, 328, 591, 625
Mph1103I ATGCAT 2 cut(s) 120, 474
MseI TTAA 1 cut(s) 485
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 1 cut(s) 277
MspR9I CCNGG 3 cut(s) 278, 321, 332
MunI CAATTG 1 cut(s) 510
MvaI CCWGG 2 cut(s) 321, 332
MvnI CGCG 1 cut(s) 591
MwoI GCNNNNNNNGC 2 cut(s) 178, 478
NciI CCSGG 1 cut(s) 278
NdeI CATATG 1 cut(s) 37
NdeII GATC 2 cut(s) 418, 556
NlaIII CATG 2 cut(s) 305, 507
NmuCI GTSAC 1 cut(s) 323
NsiI ATGCAT 2 cut(s) 120, 474
PflMI CCANNNNNTGG 1 cut(s) 392
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
Psp6I CCWGG 2 cut(s) 319, 330
PspGI CCWGG 2 cut(s) 319, 330
PspPI GGNCC 1 cut(s) 500
PstI CTGCAG 1 cut(s) 431
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 2 cut(s) 418, 556
Sau96I GGNCC 1 cut(s) 500
ScaI AGTACT 1 cut(s) 355
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 3 cut(s) 278, 321, 332
SetI ASST 9 cut(s) 63, 183, 216, 271, 286, 325, 499, 520, 627
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 2 cut(s) 246, 427
SinI GGWCC 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 344
SpeI ACTAGT 2 cut(s) 350, 568
Sse9I AATT 9 cut(s) 42, 49, 89, 132, 272, 510, 545, 576, 584
SsiI CCGC 2 cut(s) 539, 612
SspMI CTAG 3 cut(s) 174, 351, 569
StyD4I CCNGG 3 cut(s) 276, 319, 330
TaaI ACNGT 3 cut(s) 113, 250, 643
TaiI ACGT 3 cut(s) 271, 499, 627
TasI AATT 9 cut(s) 42, 49, 89, 132, 272, 510, 545, 576, 584
TatI WGTACW 1 cut(s) 353
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 3 cut(s) 116, 436, 646
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 2 cut(s) 54, 287
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 3 cut(s) 116, 436, 646
Van91I CCANNNNNTGG 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 500
XagI CCTNNNNNAGG 1 cut(s) 318
XapI RAATTY 3 cut(s) 42, 49, 576
XspI CTAG 3 cut(s) 174, 351, 569
ZrmI AGTACT 1 cut(s) 355
Zsp2I ATGCAT 2 cut(s) 120, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.