Rh5BG225300

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013014
Physical Location & Seq
Reverse (-)
26166704 .. 26170076
3373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG225300.1

Sequence Viewer

Length: 711 bp
ATGCTGCTGTTGAATTGCTGGCTACTTGTGAACACTGTATGCATCTCTCATCAGCAATTCAATATGTTGGAGATAACTACGAACCCCCTCCAGAGGTCAGGTTCTTGCATTTTTATTCATCGTGCAAAGTTTTGGTTCAGTCACTTAAGTGACTCTGCTTATTCATGTGCTAATCTGTACTCCATCAAACTGATATGTTTATCTGGTATTCAATATCAGCCTAATCATCTTACTGATTTTGAGAAGTTGCTCAAAAATGAGGTTGCAAAGCAAAAGGCTAAATCATCTTCAACTACAGTAAACCATTCTATGATACGTCAATTCCGGGAAGCTGTCTGGAATGTTCATCATGCAGGACAGCCTATGCCAGGTGACGACCAGGAGGACATCGTAATGACTAGTACTGATGGCAATCTTCGGAATACCACTTGCCCAGTTAGTGGAAAGGCTATCACGGATTTGGAAGATCCAGTCTGCAGTGTTGACTGCAAGCATATATATCAAACAAAGGAGATTATGCATTACTTGCAGTTAAAGGGGGGACGTGGACCATGCCCAATTGCAGGTTGTCCTAAGATGTTGCAGGTGGACAAATTGGTTCGCGATCCATTGCTACTAGTTGAAATTCAAGAATTGCGTGATATGAGGAAGCACCAGACCGCTGATACTAACGTGATAGAGGATTTCACTGAGCTGGATGAAGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

236

Amino Acids

26.82

Weight (kDa)

5.91

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Nse PF11789 129 - 190 1.9e-08 Zinc-finger of the MIZ type in Nse subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 574
Acc36I ACCTGC 2 cut(s) 554, 574
AccB7I CCANNNNNTGG 1 cut(s) 440
AccII CGCG 1 cut(s) 603
AciI CCGC 1 cut(s) 660
AclWI GGATC 2 cut(s) 461, 599
AcsI RAATTY 1 cut(s) 624
AfaI GTAC 2 cut(s) 179, 403
AfiI CCNNNNNNNGG 4 cut(s) 93, 368, 440, 563
AflII CTTAAG 1 cut(s) 145
AgsI TTSAA 6 cut(s) 13, 61, 212, 291, 623, 629
AhlI ACTAGT 2 cut(s) 398, 616
AjiI CACGTC 1 cut(s) 545
AjnI CCWGG 2 cut(s) 367, 378
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 2 cut(s) 332, 694
AluI AGCT 2 cut(s) 332, 694
AlwI GGATC 2 cut(s) 461, 599
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 624
AspS9I GGNCC 1 cut(s) 548
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 383
AvaII GGWCC 1 cut(s) 548
BaeI ACNNNNGTAYC 2 cut(s) 657, 690
BccI CCATC 2 cut(s) 191, 401
BciT130I CCWGG 2 cut(s) 369, 380
BcnI CCSGG 1 cut(s) 326
BcuI ACTAGT 2 cut(s) 398, 616
BfaI CTAG 2 cut(s) 399, 617
BfmI CTRYAG 2 cut(s) 294, 475
BfrI CTTAAG 1 cut(s) 145
BfuAI ACCTGC 2 cut(s) 554, 574
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 3 cut(s) 326, 369, 380
Bme18I GGWCC 1 cut(s) 548
BmgBI CACGTC 1 cut(s) 545
BmgT120I GGNCC 1 cut(s) 548
BmrFI CCNGG 3 cut(s) 326, 369, 380
BmrI ACTGGG 1 cut(s) 428
BmsI GCATC 1 cut(s) 51
BmuI ACTGGG 1 cut(s) 428
BpmI CTGGAG 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 326
BsaBI GATNNNNATC 1 cut(s) 411
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 368, 440, 563
Bse1I ACTGG 2 cut(s) 434, 470
Bse3DI GCAATG 1 cut(s) 608
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 2 cut(s) 369, 380
BseGI GGATG 1 cut(s) 703
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 4 cut(s) 93, 368, 440, 563
BseMI GCAATG 1 cut(s) 608
BseMII CTCAG 1 cut(s) 681
BseNI ACTGG 2 cut(s) 434, 470
Bsh1236I CGCG 1 cut(s) 603
BsiSI CCGG 1 cut(s) 325
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 4 cut(s) 93, 368, 440, 563
BsmFI GGGAC 1 cut(s) 555
Bsp143I GATC 2 cut(s) 466, 604
Bsp68I TCGCGA 1 cut(s) 603
BspACI CCGC 1 cut(s) 660
BspCNI CTCAG 1 cut(s) 682
BspFNI CGCG 1 cut(s) 603
BspMAI CTGCAG 1 cut(s) 479
BspMI ACCTGC 2 cut(s) 554, 574
BspPI GGATC 2 cut(s) 461, 599
BspTI CTTAAG 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 608
BsrI ACTGG 2 cut(s) 434, 470
BssMI GATC 2 cut(s) 466, 604
Bst2UI CCWGG 2 cut(s) 369, 380
Bst4CI ACNGT 2 cut(s) 37, 298
BstAFI CTTAAG 1 cut(s) 145
BstAPI GCANNNNNTGC 1 cut(s) 526
BstC8I GCNNGC 2 cut(s) 20, 491
BstDEI CTNAG 2 cut(s) 573, 690
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 703
BstFNI CGCG 1 cut(s) 603
BstKTI GATC 2 cut(s) 469, 607
BstMBI GATC 2 cut(s) 466, 604
BstMWI GCNNNNNNNGC 1 cut(s) 526
BstNI CCWGG 2 cut(s) 369, 380
BstSCI CCNGG 3 cut(s) 324, 367, 378
BstSFI CTRYAG 2 cut(s) 294, 475
BstUI CGCG 1 cut(s) 603
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BtrI CACGTC 1 cut(s) 545
BtsCI GGATG 1 cut(s) 703
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 3 cut(s) 33, 484, 687
BtuMI TCGCGA 1 cut(s) 603
BveI ACCTGC 2 cut(s) 554, 574
Cac8I GCNNGC 2 cut(s) 20, 491
Cfr13I GGNCC 1 cut(s) 548
Csp6I GTAC 2 cut(s) 178, 402
CviAII CATG 3 cut(s) 165, 350, 552
CviJI RGCY 7 cut(s) 22, 220, 278, 332, 361, 449, 694
CviKI_1 RGCY 7 cut(s) 22, 220, 278, 332, 361, 449, 694
CviQI GTAC 2 cut(s) 178, 402
DdeI CTNAG 2 cut(s) 573, 690
DpnI GATC 2 cut(s) 468, 606
DpnII GATC 2 cut(s) 466, 604
Eco47I GGWCC 1 cut(s) 548
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoRII CCWGG 2 cut(s) 367, 378
EcoT22I ATGCAT 2 cut(s) 44, 522
FaeI CATG 3 cut(s) 168, 353, 555
FaqI GGGAC 1 cut(s) 555
FatI CATG 3 cut(s) 164, 349, 551
Fnu4HI GCNGC 1 cut(s) 5
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 2 cut(s) 399, 617
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 1 cut(s) 74
HapII CCGG 1 cut(s) 325
Hin1II CATG 3 cut(s) 168, 353, 555
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 1 cut(s) 152
HpaII CCGG 1 cut(s) 325
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 5 cut(s) 31, 301, 484, 548, 589
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 4 cut(s) 91, 337, 602, 629
Hpy8I GTNNAC 5 cut(s) 31, 301, 484, 548, 589
HpyCH4III ACNGT 2 cut(s) 37, 298
HpyCH4IV ACGT 3 cut(s) 316, 544, 672
HpyF10VI GCNNNNNNNGC 1 cut(s) 526
HpyF3I CTNAG 2 cut(s) 573, 690
HpySE526I ACGT 3 cut(s) 316, 544, 672
Hsp92II CATG 3 cut(s) 168, 353, 555
Kzo9I GATC 2 cut(s) 466, 604
LweI GCATC 1 cut(s) 51
MaeI CTAG 2 cut(s) 399, 617
MaeII ACGT 3 cut(s) 316, 544, 672
MaeIII GTNAC 3 cut(s) 140, 149, 371
MalI GATC 2 cut(s) 468, 606
MboI GATC 2 cut(s) 466, 604
MboII GAAGA 3 cut(s) 279, 407, 476
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 1 cut(s) 466
MluCI AATT 7 cut(s) 13, 56, 320, 558, 593, 624, 632
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 6 cut(s) 87, 98, 253, 376, 639, 673
Mph1103I ATGCAT 2 cut(s) 44, 522
MseI TTAA 3 cut(s) 146, 533, 709
MslI CAYNNNNRTG 2 cut(s) 147, 392
MspA1I CMGCKG 1 cut(s) 662
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 1 cut(s) 325
MspR9I CCNGG 3 cut(s) 326, 369, 380
MunI CAATTG 1 cut(s) 558
MvaI CCWGG 2 cut(s) 369, 380
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 1 cut(s) 526
NciI CCSGG 1 cut(s) 326
NdeII GATC 2 cut(s) 466, 604
NlaIII CATG 3 cut(s) 168, 353, 555
NmuCI GTSAC 3 cut(s) 140, 149, 371
NruI TCGCGA 1 cut(s) 603
NsiI ATGCAT 2 cut(s) 44, 522
OliI CACNNNNGTG 1 cut(s) 147
PaqCI CACCTGC 1 cut(s) 574
PflMI CCANNNNNTGG 1 cut(s) 440
PfoI TCCNGGA 1 cut(s) 324
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Psp6I CCWGG 2 cut(s) 367, 378
PspGI CCWGG 2 cut(s) 367, 378
PspPI GGNCC 1 cut(s) 548
PstI CTGCAG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 466
RruI TCGCGA 1 cut(s) 603
RsaI GTAC 2 cut(s) 179, 403
RsaNI GTAC 2 cut(s) 178, 402
RseI CAYNNNNRTG 2 cut(s) 147, 392
SaqAI TTAA 3 cut(s) 146, 533, 709
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 2 cut(s) 466, 604
Sau96I GGNCC 1 cut(s) 548
ScaI AGTACT 1 cut(s) 403
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 3 cut(s) 326, 369, 380
SfaNI GCATC 1 cut(s) 51
SfcI CTRYAG 2 cut(s) 294, 475
SinI GGWCC 1 cut(s) 548
SmiMI CAYNNNNRTG 2 cut(s) 147, 392
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
SpeI ACTAGT 2 cut(s) 398, 616
Sse9I AATT 7 cut(s) 13, 56, 320, 558, 593, 624, 632
SsiI CCGC 1 cut(s) 660
SspMI CTAG 2 cut(s) 399, 617
StyD4I CCNGG 3 cut(s) 324, 367, 378
TaaI ACNGT 2 cut(s) 37, 298
TaiI ACGT 3 cut(s) 319, 547, 675
TasI AATT 7 cut(s) 13, 56, 320, 558, 593, 624, 632
TatI WGTACW 2 cut(s) 177, 401
Tru1I TTAA 3 cut(s) 146, 533, 709
Tru9I TTAA 3 cut(s) 146, 533, 709
TscAI CASTG 3 cut(s) 40, 484, 694
TseFI GTSAC 3 cut(s) 140, 149, 371
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 3 cut(s) 140, 149, 371
TspDTI ATGAA 3 cut(s) 107, 153, 335
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 3 cut(s) 40, 484, 694
Van91I CCANNNNNTGG 1 cut(s) 440
Vha464I CTTAAG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 548
XagI CCTNNNNNAGG 1 cut(s) 366
XapI RAATTY 1 cut(s) 624
XspI CTAG 2 cut(s) 399, 617
ZrmI AGTACT 1 cut(s) 403
Zsp2I ATGCAT 2 cut(s) 44, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.