MD11G1255800.v1.1

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013014
Physical Location & Seq
Forward (+)
36870235 .. 36873427
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1255800.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGCCTTGACCTCTCGCACTCATGGCGTCACTGGACGGATCCGAACCGCCACATCCAATCTCCACGAGGACAACCAGTCTCTTATCTCCGATATTGGGAAAGCTTTCAGTTTGATGAAGGAAATTGCAGTTGATTTTGAAAGGGCTAAGCAGTTTGAGATGGTGAAAGAGCTCGAAAATGCCGTTGTTGAATTGGTGGATACTTATGAAGACTGTTCGCATTTTTCGTCGGCAATTGAATCTGTTGGAGTTATATACCAACCTGGGCCAGAGCTTACGGATTTCGATAAGTTGTTCAAGAATGAGGTTGCACAGCTGAAGGCAAACTCATCTTCAGTTCCCCAAAACCATCCACTAATGCGTCAATTCCGGGAAGGTGTCTGGAAAGTTCATCATGCAGGAGAGCCTATGCCAGGTGAAGAGCAGGAAGACATTATAATGACGAGCAACCAAAGCACTATTTTGAATGTCACTTGCCCATTGAGTGGAAAGCCTGTCATTGAACTGGAGCATCCAGTTCGGAGTATCATATGCAAGCATGTTTATGACAAAGGGGCAATCACCAGTACTTACGGGGAAACAACACACGCTGCCCTGTTGCAGCTTGCCCTGCGAAATTGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

209

Amino Acids

23.11

Weight (kDa)

5.45

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Nse PF11789 144 - 191 4.8e-07 Zinc-finger of the MIZ type in Nse subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 437
AccB7I CCANNNNNTGG 1 cut(s) 485
AciI CCGC 1 cut(s) 48
AclWI GGATC 2 cut(s) 34, 47
AcuI CTGAAG 2 cut(s) 318, 338
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 1 cut(s) 568
AfiI CCNNNNNNNGG 3 cut(s) 96, 413, 485
AgsI TTSAA 6 cut(s) 140, 191, 239, 298, 466, 503
AhdI GACNNNNNGTC 1 cut(s) 76
AjnI CCWGG 2 cut(s) 262, 412
AluBI AGCT 6 cut(s) 104, 172, 274, 316, 604, 624
AluI AGCT 6 cut(s) 104, 172, 274, 316, 604, 624
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 2 cut(s) 34, 47
AoxI GGCC 2 cut(s) 3, 266
ApeKI GCWGC 3 cut(s) 590, 601, 621
Asp700I GAANNNNTTC 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 266
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 3 cut(s) 175, 428, 553
BamHI GGATCC 1 cut(s) 39
BanII GRGCYC 1 cut(s) 174
BauI CACGAG 1 cut(s) 65
BbsI GAAGAC 2 cut(s) 216, 435
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 577, 613
BccI CCATC 2 cut(s) 154, 357
BceAI ACGGC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 2 cut(s) 264, 414
BciVI GTATCC 1 cut(s) 193
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 84
BfaI CTAG 1 cut(s) 625
BfuI GTATCC 1 cut(s) 193
BisI GCNGC 3 cut(s) 591, 602, 622
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 3 cut(s) 592, 603, 623
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 3 cut(s) 264, 371, 414
BmeRI GACNNNNNGTC 1 cut(s) 76
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 1 cut(s) 41
BmrFI CCNGG 3 cut(s) 264, 371, 414
BmsI GCATC 1 cut(s) 520
BpiI GAAGAC 2 cut(s) 216, 435
BpmI CTGGAG 1 cut(s) 527
Bpu1102I GCTNAGC 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 263
Bsc4I CCNNNNNNNGG 3 cut(s) 96, 413, 485
Bse1I ACTGG 5 cut(s) 37, 76, 510, 515, 564
BseBI CCWGG 2 cut(s) 264, 414
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 3 cut(s) 53, 349, 511
BseLI CCNNNNNNNGG 3 cut(s) 96, 413, 485
BseNI ACTGG 5 cut(s) 37, 76, 510, 515, 564
BseXI GCAGC 2 cut(s) 577, 613
BshFI GGCC 2 cut(s) 5, 268
BsiHKAI GWGCWC 1 cut(s) 174
BsiSI CCGG 1 cut(s) 370
BslI CCNNNNNNNGG 3 cut(s) 96, 413, 485
BsmAI GTCTC 1 cut(s) 84
BsnI GGCC 2 cut(s) 5, 268
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 1 cut(s) 39
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 1 cut(s) 48
BspANI GGCC 2 cut(s) 5, 268
BspLI GGNNCC 1 cut(s) 41
BspPI GGATC 2 cut(s) 34, 47
BspQI GCTCTTC 1 cut(s) 414
BsrI ACTGG 5 cut(s) 37, 76, 510, 515, 564
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 1 cut(s) 39
BssNI GRCGYC 1 cut(s) 27
BssSI CACGAG 1 cut(s) 65
Bst2BI CACGAG 1 cut(s) 65
Bst2UI CCWGG 2 cut(s) 264, 414
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 1 cut(s) 414
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 536, 606
BstDEI CTNAG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 411
BstF5I GGATG 3 cut(s) 53, 349, 511
BstKTI GATC 1 cut(s) 42
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 1 cut(s) 39
BstMWI GCNNNNNNNGC 3 cut(s) 24, 453, 610
BstNI CCWGG 2 cut(s) 264, 414
BstNSI RCATGY 1 cut(s) 542
BstSCI CCNGG 3 cut(s) 262, 369, 412
BstV1I GCAGC 2 cut(s) 577, 613
BstV2I GAAGAC 2 cut(s) 216, 435
BstX2I RGATCY 1 cut(s) 39
BstYI RGATCY 1 cut(s) 39
BsuI GTATCC 1 cut(s) 193
BsuRI GGCC 2 cut(s) 5, 268
BtsCI GGATG 3 cut(s) 53, 349, 511
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 536, 606
Cfr13I GGNCC 1 cut(s) 266
CseI GACGC 2 cut(s) 16, 350
Csp6I GTAC 1 cut(s) 567
CviAII CATG 3 cut(s) 23, 395, 539
CviQI GTAC 1 cut(s) 567
DdeI CTNAG 1 cut(s) 147
DpnI GATC 1 cut(s) 41
DpnII GATC 1 cut(s) 39
DriI GACNNNNNGTC 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 414
Eam1105I GACNNNNNGTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 414
Ecl136II GAGCTC 1 cut(s) 172
Eco24I GRGCYC 1 cut(s) 174
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 318, 338
EcoICRI GAGCTC 1 cut(s) 172
EcoNI CCTNNNNNAGG 1 cut(s) 411
EcoRII CCWGG 2 cut(s) 262, 412
EcoT38I GRGCYC 1 cut(s) 174
FaeI CATG 3 cut(s) 26, 398, 542
FatI CATG 3 cut(s) 22, 394, 538
FauNDI CATATG 1 cut(s) 530
Fnu4HI GCNGC 3 cut(s) 591, 602, 622
FokI GGATG 3 cut(s) 40, 336, 498
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 3 cut(s) 591, 602, 622
FspBI CTAG 1 cut(s) 625
GluI GCNGC 3 cut(s) 591, 602, 622
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 2 cut(s) 5, 268
HapII CCGG 1 cut(s) 370
HgaI GACGC 2 cut(s) 16, 350
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 3 cut(s) 26, 398, 542
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 1 cut(s) 239
HpaII CCGG 1 cut(s) 370
HphI GGTGA 3 cut(s) 175, 428, 553
Hpy188I TCNGA 3 cut(s) 44, 91, 522
Hpy188III TCNNGA 2 cut(s) 298, 382
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 3 cut(s) 112, 313, 368
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4V TGCA 6 cut(s) 128, 311, 398, 534, 601, 621
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 453, 610
HpyF3I CTNAG 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 3 cut(s) 26, 398, 542
Kzo9I GATC 1 cut(s) 39
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 508
Lsp1109I GCAGC 2 cut(s) 577, 613
LweI GCATC 1 cut(s) 520
MaeI CTAG 1 cut(s) 625
MaeIII GTNAC 2 cut(s) 28, 469
MalI GATC 1 cut(s) 41
MboI GATC 1 cut(s) 39
MboII GAAGA 4 cut(s) 221, 324, 431, 440
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 5 cut(s) 123, 191, 234, 365, 616
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 3 cut(s) 22, 61, 298
MroXI GAANNNNTTC 1 cut(s) 104
MslI CAYNNNNRTG 2 cut(s) 437, 543
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 3 cut(s) 264, 371, 414
MunI CAATTG 1 cut(s) 234
MvaI CCWGG 2 cut(s) 264, 414
MwoI GCNNNNNNNGC 3 cut(s) 24, 453, 610
NciI CCSGG 1 cut(s) 371
NdeI CATATG 1 cut(s) 530
NdeII GATC 1 cut(s) 39
NlaIII CATG 3 cut(s) 26, 398, 542
NlaIV GGNNCC 1 cut(s) 41
NmuCI GTSAC 2 cut(s) 28, 469
NspI RCATGY 1 cut(s) 542
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 224
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 239
PflMI CCANNNNNTGG 1 cut(s) 485
PfoI TCCNGGA 1 cut(s) 369
PkrI GCNGC 3 cut(s) 592, 603, 623
PsiI TTATAA 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 262, 412
PspGI CCWGG 2 cut(s) 262, 412
PspN4I GGNNCC 1 cut(s) 41
PspPI GGNCC 1 cut(s) 266
PsuI RGATCY 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 1 cut(s) 568
RsaNI GTAC 1 cut(s) 567
RseI CAYNNNNRTG 2 cut(s) 437, 543
SacI GAGCTC 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 414
SatI GCNGC 3 cut(s) 591, 602, 622
Sau3AI GATC 1 cut(s) 39
Sau96I GGNCC 1 cut(s) 266
ScaI AGTACT 1 cut(s) 568
ScrFI CCNGG 3 cut(s) 264, 371, 414
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 1 cut(s) 520
SmiMI CAYNNNNRTG 2 cut(s) 437, 543
Sse9I AATT 5 cut(s) 123, 191, 234, 365, 616
SsiI CCGC 1 cut(s) 48
SspMI CTAG 1 cut(s) 625
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 262, 369, 412
TaaI ACNGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 174, 285
TasI AATT 5 cut(s) 123, 191, 234, 365, 616
TatI WGTACW 1 cut(s) 566
TfiI GAWTC 1 cut(s) 239
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 2 cut(s) 28, 469
TseI GCWGC 3 cut(s) 590, 601, 621
Tsp45I GTSAC 2 cut(s) 28, 469
TspDTI ATGAA 3 cut(s) 131, 222, 380
TspGWI ACGGA 2 cut(s) 52, 293
TspRI CASTG 1 cut(s) 37
Van91I CCANNNNNTGG 1 cut(s) 485
XagI CCTNNNNNAGG 1 cut(s) 411
XceI RCATGY 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 1 cut(s) 625
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.