Rh5DG230000

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013014
Physical Location & Seq
Reverse (-)
26051661 .. 26055443
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG230000.1

Sequence Viewer

Length: 648 bp
ATGGCCTCGACCTCCTCTCGGACTCATGGCATCGCCGGTCGGATCCGAACCGCCGCAACCAATCTTTACTCCGACAACCAGTCCCTGCTCGGCGATATTCGAAAATCTTTCAATGTGTTGAAGGAAGTTGCTGTTGATTTGGAGAGAACTAATCAGTTTGACAAGGTGAAGGAGATTGAAAATGCTGCTGTTGAATTGCTGGCTACTTGTGAACACTGTATGCATCTCTCATCAGCAATTCAATATGTTGGAGATAACTACGAACCCCCTCCAGAGCCTAATCATCTTACTGATTTTGAGAAGTTGCTCAAAAATGAGGTTGCAAAGCAAAAGGCTAAATCATCTTCAACTACAGTAAACCATTCTATGATACGTCAATTCTGGGAAGCTGTCTGGAATGTTCATCATGCAGGACAGCCTATGCCAGGTGACGACCAGGAGGACATCGTAATGACTAGTACTGATGGCAATCTTCGGAATACCACTTGCCCAGTAAGTGGAAAGGCTATCACGGATTTGGAAGATCCAGTCTGCAGGTGCTGTACAAGTCCCTTCAAACGCTTCTTTGTCTGTTTTCACCTTTCAGAGAACTTGAAGTTTTTTTCTCTTCTCAGACAAAACAAAAACATTATGGTTATATTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006275 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0008284 GO:0009653 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009967 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010646 GO:0010647 GO:0010948 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022622 GO:0023051 GO:0023056 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032446 GO:0032501 GO:0032502 GO:0032875 GO:0032876 GO:0033554 GO:0036211 GO:0040008 GO:0042127 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045786 GO:0045787 GO:0045931 GO:0045934 GO:0048364 GO:0048507 GO:0048509 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051716 GO:0051726 GO:0060249 GO:0060250 GO:0060255 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0080036 GO:0080038 GO:0080090 GO:0090329 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000026 GO:2000104 GO:2000112 GO:2000113 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Nse

Protein Analysis

215

Amino Acids

24.22

Weight (kDa)

6.24

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15150
fragaria_vesca FvH4_3g19260
malus_domestica MD03G1235300.v1.1 MD11G1255800.v1.1
prunus_persica Prupe.4G174000_v2.0.a1
pyrus_communis pycom03g18180 pycom11g22610
rosa_chinensis RchiOBHm_Chr5g0032331
rosa_laevigata RLG00000033386
rosa_multiflora Rmu_sc0002290.1_g000006
rosa_roxburghii Rroxscaffold_1G00048090
rosa_rugosa Rorug05G0131000 Rorug05G0131100
rosa_samantha Rh5AG224400 Rh5BG225300 Rh5CG250800 Rh5DG230000
rosa_wichuraiana Rw5G020500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 525
AccB7I CCANNNNNTGG 1 cut(s) 497
AciI CCGC 2 cut(s) 51, 54
AclWI GGATC 3 cut(s) 37, 50, 518
AfaI GTAC 2 cut(s) 460, 544
AfiI CCNNNNNNNGG 3 cut(s) 18, 425, 497
AgsI TTSAA 8 cut(s) 112, 121, 179, 194, 242, 348, 556, 595
AhdI GACNNNNNGTC 1 cut(s) 79
AhlI ACTAGT 1 cut(s) 455
AjnI CCWGG 2 cut(s) 424, 435
AluBI AGCT 1 cut(s) 389
AluI AGCT 1 cut(s) 389
AlwI GGATC 3 cut(s) 37, 50, 518
AlwNI CAGNNNCTG 2 cut(s) 85, 540
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 178, 440, 569
AsuII TTCGAA 1 cut(s) 100
BamHI GGATCC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 172
BccI CCATC 1 cut(s) 458
BciT130I CCWGG 2 cut(s) 426, 437
BcuI ACTAGT 1 cut(s) 455
BfaI CTAG 1 cut(s) 456
BfmI CTRYAG 2 cut(s) 351, 532
BfuAI ACCTGC 1 cut(s) 525
BisI GCNGC 2 cut(s) 54, 186
BlsI GCNGC 2 cut(s) 55, 187
BmcAI AGTACT 1 cut(s) 460
Bme1390I CCNGG 2 cut(s) 426, 437
BmeRI GACNNNNNGTC 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 2 cut(s) 426, 437
BmrI ACTGGG 1 cut(s) 485
BmsI GCATC 2 cut(s) 39, 232
BmuI ACTGGG 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 255
Bpu14I TTCGAA 1 cut(s) 100
BsaBI GATNNNNATC 1 cut(s) 468
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 425, 497
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 3 cut(s) 79, 491, 527
Bse8I GATNNNNATC 1 cut(s) 468
BseBI CCWGG 2 cut(s) 426, 437
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 3 cut(s) 18, 425, 497
BseMII CTCAG 1 cut(s) 625
BseNI ACTGG 3 cut(s) 79, 491, 527
BseRI GAGGAG 1 cut(s) 4
BseXI GCAGC 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 2 cut(s) 67, 534
BslI CCNNNNNNNGG 3 cut(s) 18, 425, 497
BsmFI GGGAC 2 cut(s) 67, 534
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 100
Bsp1407I TGTACA 1 cut(s) 542
Bsp143I GATC 2 cut(s) 42, 523
BspACI CCGC 2 cut(s) 51, 54
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 624
BspLI GGNNCC 1 cut(s) 44
BspMAI CTGCAG 1 cut(s) 536
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 3 cut(s) 37, 50, 518
BspT104I TTCGAA 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 35
BsrGI TGTACA 1 cut(s) 542
BsrI ACTGG 3 cut(s) 79, 491, 527
BssAI RCCGGY 1 cut(s) 35
BssMI GATC 2 cut(s) 42, 523
Bst2UI CCWGG 2 cut(s) 426, 437
Bst4CI ACNGT 2 cut(s) 218, 355
Bst6I CTCTTC 1 cut(s) 612
BstAUI TGTACA 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 611
BstENI CCTNNNNNAGG 1 cut(s) 423
BstKTI GATC 2 cut(s) 45, 526
BstMBI GATC 2 cut(s) 42, 523
BstMCI CGRYCG 1 cut(s) 40
BstNI CCWGG 2 cut(s) 426, 437
BstSCI CCNGG 2 cut(s) 424, 435
BstSFI CTRYAG 2 cut(s) 351, 532
BstV1I GCAGC 1 cut(s) 172
BstX2I RGATCY 2 cut(s) 42, 523
BstYI RGATCY 2 cut(s) 42, 523
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 214
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 1 cut(s) 201
CaiI CAGNNNCTG 2 cut(s) 85, 540
Cfr10I RCCGGY 1 cut(s) 35
Csp6I GTAC 2 cut(s) 459, 543
CviAII CATG 2 cut(s) 26, 407
CviJI RGCY 7 cut(s) 5, 203, 277, 335, 389, 418, 506
CviKI_1 RGCY 7 cut(s) 5, 203, 277, 335, 389, 418, 506
CviQI GTAC 2 cut(s) 459, 543
DdeI CTNAG 1 cut(s) 611
DpnI GATC 2 cut(s) 44, 525
DpnII GATC 2 cut(s) 42, 523
DriI GACNNNNNGTC 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 612
Eam1105I GACNNNNNGTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 612
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoRII CCWGG 2 cut(s) 424, 435
EcoT22I ATGCAT 1 cut(s) 225
FaeI CATG 2 cut(s) 29, 410
FaiI YATR 8 cut(s) 27, 221, 246, 368, 408, 422, 632, 638
FaqI GGGAC 2 cut(s) 67, 534
FatI CATG 2 cut(s) 25, 406
Fnu4HI GCNGC 2 cut(s) 54, 186
Fsp4HI GCNGC 2 cut(s) 54, 186
FspBI CTAG 1 cut(s) 456
GluI GCNGC 2 cut(s) 54, 186
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 36
Hin1II CATG 2 cut(s) 29, 410
HinfI GANTC 1 cut(s) 22
HpaII CCGG 1 cut(s) 36
HphI GGTGA 3 cut(s) 178, 440, 569
Hpy166II GTNNAC 2 cut(s) 212, 358
Hpy188I TCNGA 7 cut(s) 21, 42, 47, 73, 477, 586, 614
Hpy188III TCNNGA 2 cut(s) 272, 394
Hpy8I GTNNAC 2 cut(s) 212, 358
HpyAV CCTTC 3 cut(s) 115, 163, 562
HpyCH4III ACNGT 2 cut(s) 218, 355
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 5 cut(s) 223, 323, 410, 534, 643
HpyF3I CTNAG 1 cut(s) 611
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 2 cut(s) 29, 410
Kzo9I GATC 2 cut(s) 42, 523
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 2 cut(s) 39, 232
MaeI CTAG 1 cut(s) 456
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 428
MalI GATC 2 cut(s) 44, 525
MboI GATC 2 cut(s) 42, 523
MboII GAAGA 4 cut(s) 336, 464, 533, 599
MflI RGATCY 2 cut(s) 42, 523
MluCI AATT 3 cut(s) 194, 237, 377
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 3 cut(s) 20, 96, 229
MnlI CCTC 6 cut(s) 16, 22, 25, 279, 310, 433
Mph1103I ATGCAT 1 cut(s) 225
MslI CAYNNNNRTG 1 cut(s) 449
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 426, 437
MvaI CCWGG 2 cut(s) 426, 437
NdeII GATC 2 cut(s) 42, 523
NlaIII CATG 2 cut(s) 29, 410
NlaIV GGNNCC 1 cut(s) 44
NmeAIII GCCGAG 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 428
NsiI ATGCAT 1 cut(s) 225
NspV TTCGAA 1 cut(s) 100
PaqCI CACCTGC 1 cut(s) 525
PflMI CCANNNNNTGG 1 cut(s) 497
PkrI GCNGC 2 cut(s) 55, 187
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 2 cut(s) 424, 435
PspGI CCWGG 2 cut(s) 424, 435
PspN4I GGNNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 536
PstNI CAGNNNCTG 2 cut(s) 85, 540
PsuI RGATCY 2 cut(s) 42, 523
RsaI GTAC 2 cut(s) 460, 544
RsaNI GTAC 2 cut(s) 459, 543
RseI CAYNNNNRTG 1 cut(s) 449
SatI GCNGC 2 cut(s) 54, 186
Sau3AI GATC 2 cut(s) 42, 523
ScaI AGTACT 1 cut(s) 460
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 426, 437
SetI ASST 8 cut(s) 14, 168, 321, 376, 391, 430, 539, 582
SfaNI GCATC 2 cut(s) 39, 232
SfcI CTRYAG 2 cut(s) 351, 532
SfuI TTCGAA 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 449
SpeI ACTAGT 1 cut(s) 455
Sse9I AATT 3 cut(s) 194, 237, 377
SsiI CCGC 2 cut(s) 51, 54
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 424, 435
TaaI ACNGT 2 cut(s) 218, 355
TaiI ACGT 1 cut(s) 376
TaqI TCGA 2 cut(s) 8, 100
TasI AATT 3 cut(s) 194, 237, 377
TatI WGTACW 2 cut(s) 458, 542
TauI GCSGC 1 cut(s) 56
TscAI CASTG 1 cut(s) 221
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 1 cut(s) 392
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 497
XagI CCTNNNNNAGG 1 cut(s) 423
XspI CTAG 1 cut(s) 456
ZrmI AGTACT 1 cut(s) 460
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.