MD03G1242500.v1.1

mitochondrial gene expression

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012232
Physical Location & Seq
Reverse (-)
32713669 .. 32718925
5257 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1242500.v1.1.491

Sequence Viewer

Length: 597 bp
ATGAACGAATACATCGGTGATGAAACAAACAATGCACAAGAACAAAAGGAACATGAAATAGAAGACCCCGAGCTGTGTAGACTTCTTGTACCAAAAGCAGAAGATCTGCCTCTCATCCCTCCCTCTTCTGTTGAATCCAACTTTGTCTCTTATTTCGCTCCAGATTTTATGAAGCCAGGACATGACCAGTACATTTATCGCCATGCCAATGGATTGTGTGTGATTGGCTTGGCTCCAACACATGTGGCTCTTAAGGAAGAAGGGGGTATCACAGCTGTTGATTTCAATGTTGGGAAATCTGATCGTAGTGGGATAAAGGTTACTGGAAAACGCAAGAAGAATGCACAACACTTGGAGTCCAACTCAGCCTTGTGTAAAGTTTGCACCAATGATGCTACTTATATTGTGAGGTGTTGCGTAAAAGGTTCACTATTGGAAGTGAATGATAGATTAATCAAGCAGCCAGATTTGCTTAATTCAGCAGCAGATAGAGAAGGATATATTGCCATTATCATGCCAAAGCCAGCAGATTGGCTCAAAGTCAAGGATTCGCTGTTAAGCCTTGAAGATTACAAAAAGCTGAGGGAAGTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCV_H

Protein Analysis

199

Amino Acids

22.07

Weight (kDa)

5.59

Isoelectric Point (pI)

25.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_H PF01597 89 - 194 5.3e-06 Glycine cleavage H-protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012232)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75980 AT1G75980
fragaria_vesca FvH4_3g18360
malus_domestica MD03G1242500.v1.1
prunus_persica Prupe.4G165400_v2.0.a1
pyrus_communis pycom11g23170
rosa_chinensis RchiOBHm_Chr5g0031011
rosa_laevigata RLG00000033280
rosa_multiflora Rmu_sc0001523.1_g000003
rosa_roxburghii Rroxscaffold_1G00049220 Rroxscaffold_1G00071310
rosa_rugosa Rorug05G0122300
rosa_samantha Rh4DG170500 Rh5BG214600 Rh5CG238400 Rh5DG219100
rosa_wichuraiana Rw3G027050 Rw5G019710 Rw6G016970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 79
AfaI GTAC 2 cut(s) 90, 191
AflII CTTAAG 1 cut(s) 251
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 3 cut(s) 134, 286, 566
AjnI CCWGG 1 cut(s) 175
AluBI AGCT 3 cut(s) 73, 275, 580
AluI AGCT 3 cut(s) 73, 275, 580
Alw26I GTCTC 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 68
ApeKI GCWGC 2 cut(s) 460, 482
AseI ATTAAT 1 cut(s) 452
AsuHPI GGTGA 1 cut(s) 29
AvaI CYCGRG 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 69
BbvCI CCTCAGC 1 cut(s) 581
BbvI GCAGC 2 cut(s) 472, 494
BciT130I CCWGG 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 151
BfrI CTTAAG 1 cut(s) 251
BglII AGATCT 1 cut(s) 103
BisI GCNGC 2 cut(s) 461, 483
BlsI GCNGC 2 cut(s) 462, 484
Bme1390I CCNGG 1 cut(s) 177
BmeT110I CYCGRG 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 382
BpiI GAAGAC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 581
Bse1I ACTGG 2 cut(s) 187, 328
BseBI CCWGG 1 cut(s) 177
BseGI GGATG 1 cut(s) 114
BseMII CTCAG 2 cut(s) 378, 572
BseNI ACTGG 2 cut(s) 187, 328
BseXI GCAGC 2 cut(s) 472, 494
BsiHKCI CYCGRG 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 346
BsoBI CYCGRG 1 cut(s) 68
Bsp143I GATC 2 cut(s) 103, 301
BspCNI CTCAG 2 cut(s) 377, 573
BspHI TCATGA 1 cut(s) 593
BspLI GGNNCC 1 cut(s) 234
BspTI CTTAAG 1 cut(s) 251
BsrI ACTGG 2 cut(s) 187, 328
BssMI GATC 2 cut(s) 103, 301
Bst2UI CCWGG 1 cut(s) 177
Bst6I CTCTTC 1 cut(s) 130
BstAFI CTTAAG 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 2 cut(s) 364, 581
BstF5I GGATG 1 cut(s) 114
BstKTI GATC 2 cut(s) 106, 304
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 2 cut(s) 103, 301
BstMWI GCNNNNNNNGC 1 cut(s) 469
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 472, 494
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 103
BstXI CCANNNNNNTGG 2 cut(s) 209, 531
BstYI RGATCY 1 cut(s) 103
BtsCI GGATG 1 cut(s) 114
Cac8I GCNNGC 1 cut(s) 525
CciI TCATGA 1 cut(s) 593
Csp6I GTAC 2 cut(s) 89, 190
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviAII CATG 6 cut(s) 53, 182, 203, 242, 514, 594
CviQI GTAC 2 cut(s) 89, 190
DdeI CTNAG 2 cut(s) 364, 581
DpnI GATC 2 cut(s) 105, 303
DpnII GATC 2 cut(s) 103, 301
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 175
FaeI CATG 6 cut(s) 56, 185, 206, 245, 517, 597
FaiI YATR 9 cut(s) 54, 170, 183, 204, 243, 402, 501, 515, 595
FatI CATG 6 cut(s) 52, 181, 202, 241, 513, 593
FblI GTMKAC 1 cut(s) 79
Fnu4HI GCNGC 2 cut(s) 461, 483
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 461, 483
GluI GCNGC 2 cut(s) 461, 483
GsuI CTGGAG 1 cut(s) 144
Hin1II CATG 6 cut(s) 56, 185, 206, 245, 517, 597
HinfI GANTC 3 cut(s) 134, 356, 548
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 2 cut(s) 80, 428
Hpy188I TCNGA 1 cut(s) 301
Hpy188III TCNNGA 2 cut(s) 161, 594
Hpy8I GTNNAC 2 cut(s) 80, 428
HpyAV CCTTC 2 cut(s) 254, 488
HpyCH4V TGCA 3 cut(s) 35, 344, 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 469
HpyF3I CTNAG 2 cut(s) 364, 581
Hsp92II CATG 6 cut(s) 56, 185, 206, 245, 517, 597
Kzo9I GATC 2 cut(s) 103, 301
LmnI GCTCC 2 cut(s) 163, 238
LpnPI CCDG 7 cut(s) 162, 174, 189, 200, 309, 477, 537
Lsp1109I GCAGC 2 cut(s) 472, 494
LweI GCATC 1 cut(s) 382
MaeIII GTNAC 1 cut(s) 319
MalI GATC 2 cut(s) 105, 303
MboI GATC 2 cut(s) 103, 301
MboII GAAGA 6 cut(s) 74, 113, 117, 269, 349, 578
MflI RGATCY 1 cut(s) 103
MluCI AATT 1 cut(s) 475
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 3 cut(s) 162, 260, 384
MnlI CCTC 5 cut(s) 120, 129, 133, 402, 576
MseI TTAA 4 cut(s) 252, 452, 474, 557
MslI CAYNNNNRTG 2 cut(s) 207, 512
MspA1I CMGCKG 1 cut(s) 275
MspCI CTTAAG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 469
NdeII GATC 2 cut(s) 103, 301
NlaIII CATG 6 cut(s) 56, 185, 206, 245, 517, 597
NlaIV GGNNCC 1 cut(s) 234
NspI RCATGY 1 cut(s) 245
PagI TCATGA 1 cut(s) 593
PciI ACATGT 1 cut(s) 241
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 2 cut(s) 134, 548
PkrI GCNGC 2 cut(s) 462, 484
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PscI ACATGT 1 cut(s) 241
PshBI ATTAAT 1 cut(s) 452
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 1 cut(s) 234
PsuI RGATCY 1 cut(s) 103
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 2 cut(s) 90, 191
RsaNI GTAC 2 cut(s) 89, 190
RseI CAYNNNNRTG 2 cut(s) 207, 512
SaqAI TTAA 4 cut(s) 252, 452, 474, 557
SatI GCNGC 2 cut(s) 461, 483
Sau3AI GATC 2 cut(s) 103, 301
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 1 cut(s) 177
SetI ASST 6 cut(s) 75, 277, 321, 413, 427, 582
SfaNI GCATC 1 cut(s) 382
SmiMI CAYNNNNRTG 2 cut(s) 207, 512
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 1 cut(s) 475
StyD4I CCNGG 1 cut(s) 175
TasI AATT 1 cut(s) 475
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 2 cut(s) 134, 548
Tru1I TTAA 4 cut(s) 252, 452, 474, 557
Tru9I TTAA 4 cut(s) 252, 452, 474, 557
TseI GCWGC 2 cut(s) 460, 482
TspDTI ATGAA 5 cut(s) 17, 36, 69, 185, 582
Vha464I CTTAAG 1 cut(s) 251
VspI ATTAAT 1 cut(s) 452
XceI RCATGY 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.