Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004064

Physical Location & Seq

Genomic Coordinates Reverse (-)

33493546 .. 33496029

Gene Structure 2484 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1247600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 918 bp

ATGAGATGCAATGCGTGTTGGCGAGAACTGGAAGGGCGAGCTGTTTCTACCACATGTGGCCACCTTTTGTGCACAGAGGATGCGGGCAAGATCCTTGGCAGTGACGGAGCGTGTCCAGTTTGTGATCAAGTGCTCTCTAAGAGTCATATGAAGGCTTTTGATATCAATCCAAATGACGAATGGATAAGTATGGCCATGATTGGAGTGTCTCCACAGATATTAATGAAGAGTGCACACAAAAGTGTCATGTTCTACATTGGCCAAAGGGAATTGGAAATGCAGTTCAAGATGAACAGAATACAAGCTCAGTATCGGCAGAAATTTGAAGCAATGCAAGAGAAGTTTGGTGAGAAAATGGAACAACTGCATGCTACATACCAGAAAATGGCGAAAAGGTGCCAAATTATGGAACAAGAAATTGAGAGCTTGTCCAAGGATAAGCAGGAGCTGCAGGAAAAGTTTTCAGAGAAATCCAGACAGAAGAGAAAATTAGATGAAATGTATGATCAATTGAGGAGTGAGTACGAGTCAGTTAAACGATCTGCCATCCAACCTTCGACCAATTTCTTTTCAAGAAACGAACCTGATATTTTCTCAAACCAAGCTAACATTATGATGGATAACAGAGAAGCTGTTAGAAAAGATTGGCCGGTTCTCACTCCTGAAACTCCAGGGCCGAAGGAGGATATATGGCCGGCGAGACAGAATAGTTCAAACTCTGGGGGACCTTTTGACATCACGCTTGAATCACCAGCAAAACAAGCTGCTATTCCAGTTGATGCTGGGGACAGAAGGGCTGGTGGTCACACTATGTTTGGAAACGGAGCGAGCAACCCCTCAATGACTCTAAGGAACATGATAATCTCCCCAATAAAGAGGCCTCAGTTCTCCCGCCGTCCCCAGATGTTCACGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000793 GO:0000794 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005712 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0034641 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048285 GO:0051026 GO:0051276 GO:0051321 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_5

Protein Analysis

306

Amino Acids

34.94

Weight (kDa)

8.6

Isoelectric Point (pI)

63.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_5	PF14634	2 - 43	1.2e-06	zinc-RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004064)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53490 AT1G53490
fragaria_vesca	FvH4_3g17740 FvH4_3g17740 FvH4_3g17740
malus_domestica	MD03G1247600.v1.1 MD11G1269900.v1.1
prunus_persica	Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1 Prupe.4G156500_v2.0.a1
pyrus_communis	pycom03g19630 pycom03g19720 pycom11g23840
rosa_chinensis	RchiOBHm_Chr5g0029521
rosa_laevigata	RLG00000033175
rosa_multiflora	Rmu_sc0004181.1_g000010
rosa_roxburghii	Rroxscaffold_1G00050450
rosa_rugosa	Rorug05G0114400 Rorug05G0114500 Rorug05G0114600
rosa_samantha	Rh5AG206600 Rh5BG205300 Rh5CG227300 Rh5DG208900
rosa_wichuraiana	Rw5G018790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	396
AccB7I	CCANNNNNTGG	2 cut(s)	385, 406
AciI	CCGC	2 cut(s)	83, 892
AclWI	GGATC	1 cut(s)	85
AcoI	YGGCCR	5 cut(s)	58, 192, 259, 647, 692
AcsI	RAATTY	1 cut(s)	320
AfaI	GTAC	1 cut(s)	524
AfiI	CCNNNNNNNGG	2 cut(s)	385, 406
AflIII	ACRYGT	1 cut(s)	53
AgsI	TTSAA	5 cut(s)	286, 326, 573, 714, 746
AjnI	CCWGG	1 cut(s)	670
AleI	CACNNNNGTG	1 cut(s)	240
AloI	GAACNNNNNNTCC	2 cut(s)	266, 298
AluBI	AGCT	7 cut(s)	41, 305, 426, 448, 605, 632, 764
AluI	AGCT	7 cut(s)	41, 305, 426, 448, 605, 632, 764
Alw21I	GWGCWC	3 cut(s)	74, 135, 235
Alw26I	GTCTC	2 cut(s)	213, 694
Alw44I	GTGCAC	2 cut(s)	70, 231
AlwI	GGATC	1 cut(s)	85
AlwNI	CAGNNNCTG	1 cut(s)	448
AoxI	GGCC	7 cut(s)	58, 192, 259, 647, 674, 692, 878
ApaLI	GTGCAC	2 cut(s)	70, 231
ApeKI	GCWGC	2 cut(s)	448, 764
ApoI	RAATTY	1 cut(s)	320
AseI	ATTAAT	1 cut(s)	221
AspS9I	GGNCC	2 cut(s)	674, 725
AsuHPI	GGTGA	2 cut(s)	359, 741
AvaII	GGWCC	1 cut(s)	725
BaeGI	GKGCMC	2 cut(s)	74, 235
BalI	TGGCCA	3 cut(s)	60, 194, 261
BanI	GGYRCC	1 cut(s)	396
Bbv12I	GWGCWC	3 cut(s)	74, 135, 235
BbvI	GCAGC	2 cut(s)	435, 751
BccI	CCATC	2 cut(s)	554, 610
BceAI	ACGGC	1 cut(s)	879
BciT130I	CCWGG	1 cut(s)	672
BclI	TGATCA	2 cut(s)	124, 505
BcoDI	GTCTC	2 cut(s)	213, 694
BfaI	CTAG	1 cut(s)	916
BfmI	CTRYAG	1 cut(s)	449
BisI	GCNGC	2 cut(s)	449, 765
BlsI	GCNGC	2 cut(s)	450, 766
Bme1390I	CCNGG	1 cut(s)	672
Bme18I	GGWCC	1 cut(s)	725
BmgT120I	GGNCC	2 cut(s)	674, 725
BmiI	GGNNCC	2 cut(s)	398, 726
BmrFI	CCNGG	1 cut(s)	672
BmsI	GCATC	2 cut(s)	70, 769
BpmI	CTGGAG	1 cut(s)	654
BsaBI	GATNNNNATC	1 cut(s)	165
BsaJI	CCNNGG	3 cut(s)	94, 432, 671
Bsc4I	CCNNNNNNNGG	2 cut(s)	385, 406
Bse118I	RCCGGY	2 cut(s)	649, 694
Bse1I	ACTGG	3 cut(s)	33, 116, 773
Bse3DI	GCAATG	2 cut(s)	16, 336
Bse8I	GATNNNNATC	1 cut(s)	165
BseBI	CCWGG	1 cut(s)	672
BseDI	CCNNGG	3 cut(s)	94, 432, 671
BseGI	GGATG	2 cut(s)	85, 546
BseJI	GATNNNNATC	1 cut(s)	165
BseLI	CCNNNNNNNGG	2 cut(s)	385, 406
BseMI	GCAATG	2 cut(s)	16, 336
BseMII	CTCAG	2 cut(s)	320, 896
BseNI	ACTGG	3 cut(s)	33, 116, 773
BseRI	GAGGAG	1 cut(s)	529
BseSI	GKGCMC	2 cut(s)	74, 235
BseXI	GCAGC	2 cut(s)	435, 751
BseYI	CCCAGC	1 cut(s)	782
BshFI	GGCC	7 cut(s)	60, 194, 261, 649, 676, 694, 880
BshNI	GGYRCC	1 cut(s)	396
BsiHKAI	GWGCWC	3 cut(s)	74, 135, 235
BsiSI	CCGG	2 cut(s)	650, 695
BslFI	GGGAC	3 cut(s)	738, 800, 882
BslI	CCNNNNNNNGG	2 cut(s)	385, 406
BsmAI	GTCTC	2 cut(s)	213, 694
BsmFI	GGGAC	3 cut(s)	738, 800, 882
BsnI	GGCC	7 cut(s)	60, 194, 261, 649, 676, 694, 880
Bsp1286I	GDGCHC	3 cut(s)	74, 135, 235
Bsp143I	GATC	4 cut(s)	90, 124, 505, 539
BspACI	CCGC	2 cut(s)	83, 892
BspANI	GGCC	7 cut(s)	60, 194, 261, 649, 676, 694, 880
BspCNI	CTCAG	2 cut(s)	319, 895
BspLI	GGNNCC	2 cut(s)	398, 726
BspMAI	CTGCAG	1 cut(s)	453
BspPI	GGATC	1 cut(s)	85
BspT107I	GGYRCC	1 cut(s)	396
BsrDI	GCAATG	2 cut(s)	16, 336
BsrFI	RCCGGY	2 cut(s)	649, 694
BsrI	ACTGG	3 cut(s)	33, 116, 773
BssAI	RCCGGY	2 cut(s)	649, 694
BssECI	CCNNGG	3 cut(s)	94, 432, 671
BssMI	GATC	4 cut(s)	90, 124, 505, 539
BssT1I	CCWWGG	2 cut(s)	94, 432
Bst2UI	CCWGG	1 cut(s)	672
Bst6I	CTCTTC	2 cut(s)	221, 476
BstAPI	GCANNNNNTGC	1 cut(s)	448
BstC8I	GCNNGC	5 cut(s)	39, 85, 369, 696, 829
BstDEI	CTNAG	4 cut(s)	138, 306, 848, 882
BstF5I	GGATG	2 cut(s)	85, 546
BstKTI	GATC	4 cut(s)	93, 127, 508, 542
BstMAI	GTCTC	2 cut(s)	213, 694
BstMBI	GATC	4 cut(s)	90, 124, 505, 539
BstMWI	GCNNNNNNNGC	2 cut(s)	448, 761
BstNI	CCWGG	1 cut(s)	672
BstNSI	RCATGY	2 cut(s)	57, 371
BstSCI	CCNGG	1 cut(s)	670
BstSFI	CTRYAG	1 cut(s)	449
BstSLI	GKGCMC	2 cut(s)	74, 235
BstV1I	GCAGC	2 cut(s)	435, 751
BstX2I	RGATCY	1 cut(s)	90
BstYI	RGATCY	1 cut(s)	90
BsuRI	GGCC	7 cut(s)	60, 194, 261, 649, 676, 694, 880
BtsCI	GGATG	2 cut(s)	85, 546
BtsI	GCAGTG	1 cut(s)	106
BtsIMutI	CAGTG	1 cut(s)	106
Cac8I	GCNNGC	5 cut(s)	39, 85, 369, 696, 829
CaiI	CAGNNNCTG	1 cut(s)	448
Cfr10I	RCCGGY	2 cut(s)	649, 694
Cfr13I	GGNCC	2 cut(s)	674, 725
Csp6I	GTAC	1 cut(s)	523
CviAII	CATG	5 cut(s)	54, 196, 247, 368, 856
CviQI	GTAC	1 cut(s)	523
DdeI	CTNAG	4 cut(s)	138, 306, 848, 882
DpnI	GATC	4 cut(s)	92, 126, 507, 541
DpnII	GATC	4 cut(s)	90, 124, 505, 539
EaeI	YGGCCR	5 cut(s)	58, 192, 259, 647, 692
Eam1104I	CTCTTC	2 cut(s)	221, 476
EarI	CTCTTC	2 cut(s)	221, 476
Eco130I	CCWWGG	2 cut(s)	94, 432
Eco147I	AGGCCT	1 cut(s)	880
Eco32I	GATATC	1 cut(s)	163
Eco47I	GGWCC	1 cut(s)	725
EcoO109I	RGGNCCY	1 cut(s)	725
EcoRII	CCWGG	1 cut(s)	670
EcoRV	GATATC	1 cut(s)	163
EcoT14I	CCWWGG	2 cut(s)	94, 432
ErhI	CCWWGG	2 cut(s)	94, 432
FaeI	CATG	5 cut(s)	57, 199, 250, 371, 859
FaqI	GGGAC	3 cut(s)	738, 800, 882
FatI	CATG	5 cut(s)	53, 195, 246, 367, 855
FauI	CCCGC	2 cut(s)	76, 899
FauNDI	CATATG	1 cut(s)	147
FbaI	TGATCA	2 cut(s)	124, 505
Fnu4HI	GCNGC	2 cut(s)	449, 765
FokI	GGATG	2 cut(s)	92, 533
Fsp4HI	GCNGC	2 cut(s)	449, 765
FspBI	CTAG	1 cut(s)	916
GluI	GCNGC	2 cut(s)	449, 765
GsaI	CCCAGC	1 cut(s)	786
GsuI	CTGGAG	1 cut(s)	654
HaeIII	GGCC	7 cut(s)	60, 194, 261, 649, 676, 694, 880
HapII	CCGG	2 cut(s)	650, 695
Hin1II	CATG	5 cut(s)	57, 199, 250, 371, 859
HinfI	GANTC	4 cut(s)	142, 527, 746, 844
HpaII	CCGG	2 cut(s)	650, 695
HphI	GGTGA	2 cut(s)	359, 741
Hpy166II	GTNNAC	3 cut(s)	72, 233, 909
Hpy188I	TCNGA	1 cut(s)	466
Hpy188III	TCNNGA	4 cut(s)	286, 474, 573, 662
Hpy8I	GTNNAC	3 cut(s)	72, 233, 909
HpyAV	CCTTC	5 cut(s)	26, 145, 564, 673, 786
HpyCH4IV	ACGT	1 cut(s)	911
HpyCH4V	TGCA	7 cut(s)	9, 72, 233, 280, 334, 367, 451
HpyF10VI	GCNNNNNNNGC	2 cut(s)	448, 761
HpyF3I	CTNAG	4 cut(s)	138, 306, 848, 882
HpySE526I	ACGT	1 cut(s)	911
Hsp92II	CATG	5 cut(s)	57, 199, 250, 371, 859
KroI	GCCGGC	1 cut(s)	694
KroNI	GCCGGC	1 cut(s)	696
Ksp22I	TGATCA	2 cut(s)	124, 505
Kzo9I	GATC	4 cut(s)	90, 124, 505, 539
LmnI	GCTCC	3 cut(s)	107, 445, 824
Lsp1109I	GCAGC	2 cut(s)	435, 751
LweI	GCATC	2 cut(s)	70, 769
MaeI	CTAG	1 cut(s)	916
MaeII	ACGT	1 cut(s)	911
MaeIII	GTNAC	2 cut(s)	101, 803
MalI	GATC	4 cut(s)	92, 126, 507, 541
MboI	GATC	4 cut(s)	90, 124, 505, 539
MboII	GAAGA	2 cut(s)	238, 493
MfeI	CAATTG	1 cut(s)	509
MflI	RGATCY	1 cut(s)	90
MhlI	GDGCHC	3 cut(s)	74, 135, 235
MlsI	TGGCCA	3 cut(s)	60, 194, 261
MluCI	AATT	7 cut(s)	269, 320, 402, 417, 488, 509, 562
MluNI	TGGCCA	3 cut(s)	60, 194, 261
MlyI	GAGTC	3 cut(s)	151, 536, 838
MmeI	TCCRAC	1 cut(s)	574
MnlI	CCTC	6 cut(s)	70, 507, 676, 847, 870, 891
Mox20I	TGGCCA	3 cut(s)	60, 194, 261
MroNI	GCCGGC	1 cut(s)	694
MscI	TGGCCA	3 cut(s)	60, 194, 261
MseI	TTAA	2 cut(s)	221, 534
MslI	CAYNNNNRTG	2 cut(s)	240, 614
Msp20I	TGGCCA	3 cut(s)	60, 194, 261
MspI	CCGG	2 cut(s)	650, 695
MspR9I	CCNGG	1 cut(s)	672
MunI	CAATTG	1 cut(s)	509
MvaI	CCWGG	1 cut(s)	672
MwoI	GCNNNNNNNGC	2 cut(s)	448, 761
NaeI	GCCGGC	1 cut(s)	696
NdeI	CATATG	1 cut(s)	147
NdeII	GATC	4 cut(s)	90, 124, 505, 539
NgoMIV	GCCGGC	1 cut(s)	694
NlaIII	CATG	5 cut(s)	57, 199, 250, 371, 859
NlaIV	GGNNCC	2 cut(s)	398, 726
NmuCI	GTSAC	2 cut(s)	101, 803
NspI	RCATGY	2 cut(s)	57, 371
OliI	CACNNNNGTG	1 cut(s)	240
PaeI	GCATGC	1 cut(s)	371
PceI	AGGCCT	1 cut(s)	880
PciI	ACATGT	1 cut(s)	53
PdiI	GCCGGC	1 cut(s)	696
PfeI	GAWTC	1 cut(s)	746
PflMI	CCANNNNNTGG	2 cut(s)	385, 406
PkrI	GCNGC	2 cut(s)	450, 766
PleI	GAGTC	3 cut(s)	150, 535, 838
PpsI	GAGTC	3 cut(s)	150, 535, 838
PpuMI	RGGWCCY	1 cut(s)	725
PscI	ACATGT	1 cut(s)	53
PshBI	ATTAAT	1 cut(s)	221
Psp5II	RGGWCCY	1 cut(s)	725
Psp6I	CCWGG	1 cut(s)	670
PspFI	CCCAGC	1 cut(s)	782
PspGI	CCWGG	1 cut(s)	670
PspN4I	GGNNCC	2 cut(s)	398, 726
PspPI	GGNCC	2 cut(s)	674, 725
PspPPI	RGGWCCY	1 cut(s)	725
PstI	CTGCAG	1 cut(s)	453
PstNI	CAGNNNCTG	1 cut(s)	448
PsuI	RGATCY	1 cut(s)	90
RsaI	GTAC	1 cut(s)	524
RsaNI	GTAC	1 cut(s)	523
RseI	CAYNNNNRTG	2 cut(s)	240, 614
SaqAI	TTAA	2 cut(s)	221, 534
SatI	GCNGC	2 cut(s)	449, 765
Sau3AI	GATC	4 cut(s)	90, 124, 505, 539
Sau96I	GGNCC	2 cut(s)	674, 725
SchI	GAGTC	3 cut(s)	151, 536, 838
ScrFI	CCNGG	1 cut(s)	672
SduI	GDGCHC	3 cut(s)	74, 135, 235
SfaNI	GCATC	2 cut(s)	70, 769
SfcI	CTRYAG	1 cut(s)	449
SinI	GGWCC	1 cut(s)	725
SmiMI	CAYNNNNRTG	2 cut(s)	240, 614
SphI	GCATGC	1 cut(s)	371
Sse9I	AATT	7 cut(s)	269, 320, 402, 417, 488, 509, 562
SseBI	AGGCCT	1 cut(s)	880
SsiI	CCGC	2 cut(s)	83, 892
SspMI	CTAG	1 cut(s)	916
StuI	AGGCCT	1 cut(s)	880
StyD4I	CCNGG	1 cut(s)	670
StyI	CCWWGG	2 cut(s)	94, 432
TaiI	ACGT	1 cut(s)	914
TaqI	TCGA	1 cut(s)	557
TasI	AATT	7 cut(s)	269, 320, 402, 417, 488, 509, 562
TfiI	GAWTC	1 cut(s)	746
Tru1I	TTAA	2 cut(s)	221, 534
Tru9I	TTAA	2 cut(s)	221, 534
TscAI	CASTG	1 cut(s)	106
TseFI	GTSAC	2 cut(s)	101, 803
TseI	GCWGC	2 cut(s)	448, 764
Tsp45I	GTSAC	2 cut(s)	101, 803
TspDTI	ATGAA	4 cut(s)	164, 239, 305, 510
TspGWI	ACGGA	2 cut(s)	120, 837
TspRI	CASTG	1 cut(s)	106
Van91I	CCANNNNNTGG	2 cut(s)	385, 406
VneI	GTGCAC	2 cut(s)	70, 231
VpaK11BI	GGWCC	1 cut(s)	725
VspI	ATTAAT	1 cut(s)	221
XapI	RAATTY	1 cut(s)	320
XceI	RCATGY	2 cut(s)	57, 371
XcmI	CCANNNNNNNNNTGG	1 cut(s)	177
XspI	CTAG	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.