MD03G1247900.v1.1

Integral membrane protein DUF92

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008669
Physical Location & Seq
Reverse (-)
33504899 .. 33516960
12062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1247900.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGGACTTTTCCAAAATAATACTTTTTCTTCACAGACACTCAAACTCACATTCAAATCACACCAAACCCCCTCCAATTCCTTCCTTATTCCAATCTCAATCCCACCGCTCCTCCTCCTCCTCCCTCCTCATCCTCCCCATAAAATTCCTAATCCCAATCCACCCTCACCGCCACAATCGCTCCAAACAAACAATGAACAGGCCTCCCGCAGCCAGTGTGCAGAGTGCTGTGTCCCAGGCAATGAGCCTGATCCAATCGTCGCCTCCGACTTGGCAGTCCGCCGTCCTCAGCAACGTCCTCATATTTCTGCTGGGTTTTCCAATCCTGGTCTCCGGACTCTCTGCCTCTGGCATTGGAGCTGCTTTCTTGCTCGGTACTCTCACTTGGCGCGCTTTTGGGTCTTCTGGGTTTCTCCTGGTGGCCACGTACTTCGTCATTGGGACGGCAGTGACAAAGGTGAGAATGAAACAGAAGGAGGCTGAGGGGGTAGCCGAGAAGAGGAAAGGACGGAGAGGCCCGGGCAGTGTCATTGGATCCAGTGCTGCTGGCTGTGTTTGTGCTTTCCTCACAATACTTGGAGTAGGAGGAAGCGGATATTCTCGGCTTTGGCAACTAGGGTTTGTTGCCAGCTTCTGCACTAAGTTAAGTGATACTGTCTCGAGTGAGATAGGAAAGGCATATGGCAAAACCACGTACTTAGTTACAACATTGAAGGTAGTCCCAAGGGGAACAGAGGGGGCTATGAGTGTTGAGGGAACCTTTTCTGGACTTTTGGCCGCTATCGTTCTTGCATTTGTTGGTTGTGCCATGGGTGAGATAAGTGCACCTGAAGCAATGATATGCGTAATAGCTTCCCAAATAGCTAATCTTGGTGAGAGCATTATAGGTGCTGCTCTTCAAGAGAAGGAAGGATTTCGATGGCTCAACAACGATGCTGTGAATGTCATAAACATATCGATGGGCAGCATTTTGGCAGTCTTGATGCAGCAGGTTTTACTCCAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF92

Protein Analysis

336

Amino Acids

35.49

Weight (kDa)

9.86

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF92 PF01940 100 - 321 1.2e-56 Integral membrane protein DUF92
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 274
Acc36I ACCTGC 1 cut(s) 979
AccBSI CCGCTC 1 cut(s) 108
AccII CGCG 1 cut(s) 390
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 6 cut(s) 106, 169, 207, 279, 591, 777
AclWI GGATC 3 cut(s) 244, 528, 541
AcoI YGGCCR 2 cut(s) 420, 774
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 3 cut(s) 376, 428, 695
AfiI CCNNNNNNNGG 1 cut(s) 498
AgsI TTSAA 3 cut(s) 54, 712, 899
AjnI CCWGG 3 cut(s) 234, 324, 414
AluBI AGCT 4 cut(s) 359, 630, 851, 863
AluI AGCT 4 cut(s) 359, 630, 851, 863
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 2 cut(s) 334, 661
Alw44I GTGCAC 1 cut(s) 822
AlwI GGATC 3 cut(s) 244, 528, 541
AlwNI CAGNNNCTG 1 cut(s) 633
Ama87I CYCGRG 2 cut(s) 517, 658
Aor13HI TCCGGA 1 cut(s) 332
AoxI GGCC 4 cut(s) 200, 420, 514, 774
ApaLI GTGCAC 1 cut(s) 822
ApeKI GCWGC 6 cut(s) 209, 359, 542, 890, 963, 985
ApoI RAATTY 1 cut(s) 143
Asp700I GAANNNNTTC 2 cut(s) 760, 912
AspLEI GCGC 2 cut(s) 390, 392
AspS9I GGNCC 1 cut(s) 515
AsuC2I CCSGG 2 cut(s) 518, 519
AsuHPI GGTGA 4 cut(s) 158, 469, 824, 884
AvaI CYCGRG 2 cut(s) 517, 658
BaeGI GKGCMC 1 cut(s) 826
BalI TGGCCA 1 cut(s) 422
BamHI GGATCC 1 cut(s) 533
BbsI GAAGAC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 826
BbvCI CCTCAGC 2 cut(s) 287, 480
BbvI GCAGC 6 cut(s) 221, 346, 529, 877, 975, 997
BccI CCATC 2 cut(s) 912, 952
BceAI ACGGC 2 cut(s) 266, 459
BciT130I CCWGG 3 cut(s) 236, 326, 416
BcnI CCSGG 2 cut(s) 518, 519
BcoDI GTCTC 2 cut(s) 334, 661
BfaI CTAG 1 cut(s) 614
BfuAI ACCTGC 1 cut(s) 979
BisI GCNGC 7 cut(s) 210, 360, 543, 777, 891, 964, 986
BlsI GCNGC 7 cut(s) 211, 361, 544, 778, 892, 965, 987
Bme1390I CCNGG 5 cut(s) 236, 326, 416, 518, 519
BmeT110I CYCGRG 2 cut(s) 517, 658
BmgT120I GGNCC 1 cut(s) 515
BmiI GGNNCC 2 cut(s) 535, 757
BmrFI CCNGG 5 cut(s) 236, 326, 416, 518, 519
BmsI GCATC 2 cut(s) 922, 972
BpiI GAAGAC 1 cut(s) 393
BpmI CTGGAG 1 cut(s) 983
Bpu10I CCTNAGC 2 cut(s) 287, 480
BpuMI CCSGG 2 cut(s) 518, 519
Bsa29I ATCGAT 1 cut(s) 956
BsaAI YACGTR 2 cut(s) 426, 693
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 4 cut(s) 234, 517, 722, 807
BsaWI WCCGGW 1 cut(s) 332
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 164, 194
Bsc4I CCNNNNNNNGG 1 cut(s) 498
Bse1I ACTGG 2 cut(s) 213, 537
Bse3DI GCAATG 2 cut(s) 246, 840
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 3 cut(s) 236, 326, 416
BseCI ATCGAT 1 cut(s) 956
BseDI CCNNGG 4 cut(s) 234, 517, 722, 807
BseGI GGATG 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 498
BseMI GCAATG 2 cut(s) 246, 840
BseMII CTCAG 2 cut(s) 301, 471
BseNI ACTGG 2 cut(s) 213, 537
BsePI GCGCGC 1 cut(s) 388
BseRI GAGGAG 5 cut(s) 100, 103, 106, 109, 116
BseSI GKGCMC 1 cut(s) 826
BseXI GCAGC 6 cut(s) 221, 346, 529, 877, 975, 997
BseYI CCCAGC 1 cut(s) 310
BsgI GTGCAG 2 cut(s) 239, 619
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 4 cut(s) 202, 422, 516, 776
BshVI ATCGAT 1 cut(s) 956
BsiHKAI GWGCWC 1 cut(s) 826
BsiHKCI CYCGRG 2 cut(s) 517, 658
BsiSI CCGG 2 cut(s) 333, 518
BslFI GGGAC 3 cut(s) 217, 454, 704
BslI CCNNNNNNNGG 1 cut(s) 498
BsmAI GTCTC 2 cut(s) 334, 661
BsmFI GGGAC 3 cut(s) 217, 454, 704
BsnI GGCC 4 cut(s) 202, 422, 516, 776
Bso31I GGTCTC 1 cut(s) 334
BsoBI CYCGRG 2 cut(s) 517, 658
Bsp1286I GDGCHC 1 cut(s) 826
Bsp13I TCCGGA 1 cut(s) 332
Bsp143I GATC 2 cut(s) 249, 533
Bsp19I CCATGG 1 cut(s) 807
BspACI CCGC 6 cut(s) 106, 169, 207, 279, 591, 777
BspANI GGCC 4 cut(s) 202, 422, 516, 776
BspCNI CTCAG 2 cut(s) 300, 472
BspDI ATCGAT 1 cut(s) 956
BspEI TCCGGA 1 cut(s) 332
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 535, 757
BspMI ACCTGC 1 cut(s) 979
BspPI GGATC 3 cut(s) 244, 528, 541
BspQI GCTCTTC 1 cut(s) 900
BspTNI GGTCTC 1 cut(s) 334
BsrBI CCGCTC 1 cut(s) 108
BsrDI GCAATG 2 cut(s) 246, 840
BsrI ACTGG 2 cut(s) 213, 537
BssECI CCNNGG 4 cut(s) 234, 517, 722, 807
BssHII GCGCGC 1 cut(s) 388
BssMI GATC 2 cut(s) 249, 533
BssT1I CCWWGG 2 cut(s) 722, 807
Bst2UI CCWGG 3 cut(s) 236, 326, 416
Bst4CI ACNGT 1 cut(s) 655
Bst6I CTCTTC 2 cut(s) 491, 900
BstBAI YACGTR 2 cut(s) 426, 693
BstC8I GCNNGC 3 cut(s) 390, 547, 628
BstDEI CTNAG 4 cut(s) 287, 480, 639, 697
BstDSI CCRYGG 1 cut(s) 807
BstF5I GGATG 1 cut(s) 129
BstFNI CGCG 1 cut(s) 390
BstHHI GCGC 2 cut(s) 390, 392
BstKTI GATC 2 cut(s) 252, 536
BstMAI GTCTC 2 cut(s) 334, 661
BstMBI GATC 2 cut(s) 249, 533
BstMWI GCNNNNNNNGC 2 cut(s) 177, 830
BstNI CCWGG 3 cut(s) 236, 326, 416
BstSCI CCNGG 5 cut(s) 234, 324, 414, 516, 517
BstSLI GKGCMC 1 cut(s) 826
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 6 cut(s) 221, 346, 529, 877, 975, 997
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 533
BstYI RGATCY 1 cut(s) 533
Bsu15I ATCGAT 1 cut(s) 956
BsuRI GGCC 4 cut(s) 202, 422, 516, 776
BsuTUI ATCGAT 1 cut(s) 956
BtgI CCRYGG 1 cut(s) 807
BtsCI GGATG 1 cut(s) 129
BtsI GCAGTG 2 cut(s) 453, 529
BtsIMutI CAGTG 4 cut(s) 220, 453, 529, 544
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 3 cut(s) 390, 547, 628
CaiI CAGNNNCTG 1 cut(s) 633
CfoI GCGC 2 cut(s) 390, 392
Cfr13I GGNCC 1 cut(s) 515
Cfr9I CCCGGG 1 cut(s) 517
ClaI ATCGAT 1 cut(s) 956
Csp6I GTAC 3 cut(s) 375, 427, 694
CviAII CATG 1 cut(s) 808
CviQI GTAC 3 cut(s) 375, 427, 694
DdeI CTNAG 4 cut(s) 287, 480, 639, 697
DpnI GATC 2 cut(s) 251, 535
DpnII GATC 2 cut(s) 249, 533
DrdI GACNNNNNNGTC 1 cut(s) 274
DseDI GACNNNNNNGTC 1 cut(s) 274
EaeI YGGCCR 2 cut(s) 420, 774
Eam1104I CTCTTC 2 cut(s) 491, 900
EarI CTCTTC 2 cut(s) 491, 900
EciI GGCGGA 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 722, 807
Eco147I AGGCCT 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 849
Eco88I CYCGRG 2 cut(s) 517, 658
EcoRII CCWGG 3 cut(s) 234, 324, 414
EcoT14I CCWWGG 2 cut(s) 722, 807
ErhI CCWWGG 2 cut(s) 722, 807
FaeI CATG 1 cut(s) 811
FaqI GGGAC 3 cut(s) 217, 454, 704
FatI CATG 1 cut(s) 807
FauI CCCGC 1 cut(s) 214
FauNDI CATATG 1 cut(s) 679
Fnu4HI GCNGC 7 cut(s) 210, 360, 543, 777, 891, 964, 986
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 7 cut(s) 210, 360, 543, 777, 891, 964, 986
FspBI CTAG 1 cut(s) 614
GlaI GCGC 2 cut(s) 389, 391
GluI GCNGC 7 cut(s) 210, 360, 543, 777, 891, 964, 986
GsaI CCCAGC 1 cut(s) 314
GsuI CTGGAG 1 cut(s) 983
HaeIII GGCC 4 cut(s) 202, 422, 516, 776
HapII CCGG 2 cut(s) 333, 518
HhaI GCGC 2 cut(s) 390, 392
Hin1II CATG 1 cut(s) 811
Hin6I GCGC 2 cut(s) 388, 390
HinP1I GCGC 2 cut(s) 388, 390
HinfI GANTC 1 cut(s) 336
HpaII CCGG 2 cut(s) 333, 518
HphI GGTGA 4 cut(s) 158, 469, 824, 884
Hpy166II GTNNAC 1 cut(s) 824
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 6 cut(s) 333, 658, 765, 899, 979, 1000
Hpy8I GTNNAC 1 cut(s) 824
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 5 cut(s) 90, 466, 706, 898, 902
HpyCH4III ACNGT 1 cut(s) 655
HpyCH4IV ACGT 3 cut(s) 294, 425, 692
HpyCH4V TGCA 5 cut(s) 220, 636, 791, 824, 985
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 830
HpyF3I CTNAG 4 cut(s) 287, 480, 639, 697
HpySE526I ACGT 3 cut(s) 294, 425, 692
Hsp92II CATG 1 cut(s) 811
HspAI GCGC 2 cut(s) 388, 390
Kpn2I TCCGGA 1 cut(s) 332
Kzo9I GATC 2 cut(s) 249, 533
LguI GCTCTTC 1 cut(s) 900
LmnI GCTCC 3 cut(s) 113, 185, 356
Lsp1109I GCAGC 6 cut(s) 221, 346, 529, 877, 975, 997
LweI GCATC 2 cut(s) 922, 972
MaeI CTAG 1 cut(s) 614
MaeII ACGT 3 cut(s) 294, 425, 692
MaeIII GTNAC 2 cut(s) 448, 700
MalI GATC 2 cut(s) 251, 535
MbiI CCGCTC 1 cut(s) 108
MboI GATC 2 cut(s) 249, 533
MboII GAAGA 4 cut(s) 20, 393, 508, 887
MflI RGATCY 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 826
MlsI TGGCCA 1 cut(s) 422
MluCI AATT 2 cut(s) 75, 143
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 1 cut(s) 330
MmeI TCCRAC 1 cut(s) 290
Mox20I TGGCCA 1 cut(s) 422
MroI TCCGGA 1 cut(s) 332
MroXI GAANNNNTTC 2 cut(s) 760, 912
MscI TGGCCA 1 cut(s) 422
MseI TTAA 1 cut(s) 644
MslI CAYNNNNRTG 1 cut(s) 956
Msp20I TGGCCA 1 cut(s) 422
MspI CCGG 2 cut(s) 333, 518
MspR9I CCNGG 5 cut(s) 236, 326, 416, 518, 519
MvaI CCWGG 3 cut(s) 236, 326, 416
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 177, 830
NciI CCSGG 2 cut(s) 518, 519
NcoI CCATGG 1 cut(s) 807
NdeI CATATG 1 cut(s) 679
NdeII GATC 2 cut(s) 249, 533
NlaIII CATG 1 cut(s) 811
NlaIV GGNNCC 2 cut(s) 535, 757
NmeAIII GCCGAG 2 cut(s) 517, 580
NmuCI GTSAC 1 cut(s) 448
PaeR7I CTCGAG 1 cut(s) 658
PauI GCGCGC 1 cut(s) 388
PceI AGGCCT 1 cut(s) 202
PciSI GCTCTTC 1 cut(s) 900
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PdmI GAANNNNTTC 2 cut(s) 760, 912
PkrI GCNGC 7 cut(s) 211, 361, 544, 778, 892, 965, 987
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
Ppu21I YACGTR 2 cut(s) 426, 693
Psp6I CCWGG 3 cut(s) 234, 324, 414
PspFI CCCAGC 1 cut(s) 310
PspGI CCWGG 3 cut(s) 234, 324, 414
PspN4I GGNNCC 2 cut(s) 535, 757
PspPI GGNCC 1 cut(s) 515
PstNI CAGNNNCTG 1 cut(s) 633
PsuI RGATCY 1 cut(s) 533
PteI GCGCGC 1 cut(s) 388
RsaI GTAC 3 cut(s) 376, 428, 695
RsaNI GTAC 3 cut(s) 375, 427, 694
RseI CAYNNNNRTG 1 cut(s) 956
SapI GCTCTTC 1 cut(s) 900
SaqAI TTAA 1 cut(s) 644
SatI GCNGC 7 cut(s) 210, 360, 543, 777, 891, 964, 986
Sau3AI GATC 2 cut(s) 249, 533
Sau96I GGNCC 1 cut(s) 515
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 5 cut(s) 236, 326, 416, 518, 519
SduI GDGCHC 1 cut(s) 826
SfaNI GCATC 2 cut(s) 922, 972
Sfr274I CTCGAG 1 cut(s) 658
SlaI CTCGAG 1 cut(s) 658
SmaI CCCGGG 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 1 cut(s) 658
SmoI CTYRAG 1 cut(s) 658
SrfI GCCCGGGC 1 cut(s) 519
Sse9I AATT 2 cut(s) 75, 143
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 6 cut(s) 106, 169, 207, 279, 591, 777
SspMI CTAG 1 cut(s) 614
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 5 cut(s) 234, 324, 414, 516, 517
StyI CCWWGG 2 cut(s) 722, 807
TaaI ACNGT 1 cut(s) 655
TaiI ACGT 3 cut(s) 297, 428, 695
TaqI TCGA 3 cut(s) 659, 916, 956
TasI AATT 2 cut(s) 75, 143
TauI GCSGC 1 cut(s) 779
Tru1I TTAA 1 cut(s) 644
Tru9I TTAA 1 cut(s) 644
TscAI CASTG 4 cut(s) 220, 453, 529, 544
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 6 cut(s) 209, 359, 542, 890, 963, 985
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 2 cut(s) 209, 479
TspGWI ACGGA 1 cut(s) 523
TspMI CCCGGG 1 cut(s) 517
TspRI CASTG 4 cut(s) 220, 453, 529, 544
VneI GTGCAC 1 cut(s) 822
XapI RAATTY 1 cut(s) 143
XhoI CTCGAG 1 cut(s) 658
XmaI CCCGGG 1 cut(s) 517
XmnI GAANNNNTTC 2 cut(s) 760, 912
XspI CTAG 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.